Novel laccase—producing ascomycetes View Full Text


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Article Info

DATE

2017-07

AUTHORS

N. M. Myasoedova, Zh. V. Renfeld, E. V. Podieiablonskaia, A. S. Samoilova, A. M. Chernykh, T. Classen, J. Pietruszka, M. P. Kolomytseva, L. A. Golovleva

ABSTRACT

Screening of ascomycetes producing laccases during growth on agar medium or submerged cultivation in the presence of various natural sources of carbon and energy (grain crops and potato) was carried out. The conditions of submerged cultivation of the most active strains (Myrothecium roridum VKM F-3565, Stachybotrys cylindrospora VKM F-3049, and Ulocladium atrum VKM F-4302) were optimized for the purpose of increasing laccase activity. The pH-optima and substrate selectivity of laccases in the culture liquid of the strains in relation to ABTS and phenolic compounds (2,6-dimethoxyphenol, syringaldazine, ferulic acid, p-coumaryl alcohol, and coniferyl alcohol) were investigated. High laccase activity at neutral pH was shown for the culture liquids of M. roridum VKM F-3565 and S. cylindrospora VKM F-3049 strains that provides prospects for using laccases of these strains in various cell biotechnologies. More... »

PAGES

503-511

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s0026261717030110

DOI

http://dx.doi.org/10.1134/s0026261717030110

DIMENSIONS

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