Method for rapid DNA extraction from bacterial communities of different soils View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-01

AUTHORS

E. V. Zaporozhenko, N. V. Slobodova, E. S. Boulygina, I. K. Kravchenko, B. B. Kuznetsov

ABSTRACT

A method for indirect DNA extraction from various soils significantly differing in their physicochemical properties has been developed. The proposed method is based on cell desorption from soil particles using a Tris-EDTA (TE) buffer supplemented with polyvinylpolypyrrolydone (PVPP) and sodium dodecylsulfate (SDS). Methods for subsequent cell lysis and purification of DNA preparations based on alkaline lysis followed by chromatography on ion-exchange resins were described by us earlier. The purity of the DNA preparations obtained did not depend on the type of soil. It was shown that the DNA preparations can be used for the amplification of rather large fragments, e.g., sequences spanning the complete 16S rRNA gene. More... »

PAGES

105-111

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s0026261706010188

DOI

http://dx.doi.org/10.1134/s0026261706010188

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031184348


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146 schema:name Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, Bld. 2, 117811, Moscow, Russia
147 rdf:type schema:Organization
 




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