Structural principles of the wide substrate specificity of Thermoactinomyces vulgaris carboxypeptidase T. reconstruction of the carboxypeptidase B primary specificity pocket View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-04

AUTHORS

V. Kh. Akparov, A. M. Grishin, M. P. Yusupova, N. M. Ivanova, G. G. Chestukhina

ABSTRACT

Site-directed mutagenesis in the active site of Thermoactinomyces vulgaris carboxypeptidase T (CpT), which is capable of hydrolyzing both hydrophobic and positively charged substrates, resulted in five mutants: CpT1 (A243G), CpT2 (D253G/T255D), CpT3 (A243G/D253G/T255D), CpT4 (G207S/A243G/D253G/T255D), and CpT5 (G207S/A243G/T250A/D253G/T255D). These mutants step-by-step reconstruct the primary specificity pocket of carboxypeptidase B (CpB), which is capable of cleaving only positively charged C-terminal residues. All of the mutants retained the substrate specificity of the wild-type CpT. Based on comparison of three-dimensional structures of CpB and the CpT5 model, it was suggested that the lower affinity of CpT5 for positively charged substrates than the affinity of CpB could be caused by differences in nature and spatial location of Leu247 and Ile247 and of His68 and Asp65 residues in CpT and CpB, respectively, and also in location of the water molecule bound with Ala250. An additional hydrophobic region was detected in the CpT active site formed by Tyr248, Leu247, Leu203, Ala243, CH3-group of Thr250, and CO-groups of Tyr248 and Ala243, which could be responsible for binding hydrophobic substrates. Thus, notwithstanding the considerable structural similarity of CpT and pancreatic carboxypeptidases, the mechanisms underlying their substrate specificities are different. More... »

PAGES

416-423

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s0006297907040086

DOI

http://dx.doi.org/10.1134/s0006297907040086

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048915459

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17511606


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Substitution", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carboxypeptidase B", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carboxypeptidases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrophobic and Hydrophilic Interactions", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Micromonosporaceae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Substrate Specificity", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Genetika", 
          "id": "https://www.grid.ac/institutes/grid.418697.5", 
          "name": [
            "Stepanov Laboratory of Protein Chemistry, Institute of Genetics and Selection of Industrial Microorganisms, 1-yi Dorozhnyi Proezd 1, 117545, Moscow, Russia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Akparov", 
        "givenName": "V. Kh.", 
        "id": "sg:person.01302405151.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01302405151.95"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Genetika", 
          "id": "https://www.grid.ac/institutes/grid.418697.5", 
          "name": [
            "Stepanov Laboratory of Protein Chemistry, Institute of Genetics and Selection of Industrial Microorganisms, 1-yi Dorozhnyi Proezd 1, 117545, Moscow, Russia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Grishin", 
        "givenName": "A. M.", 
        "id": "sg:person.01225521163.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01225521163.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Genetika", 
          "id": "https://www.grid.ac/institutes/grid.418697.5", 
          "name": [
            "Stepanov Laboratory of Protein Chemistry, Institute of Genetics and Selection of Industrial Microorganisms, 1-yi Dorozhnyi Proezd 1, 117545, Moscow, Russia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yusupova", 
        "givenName": "M. P.", 
        "id": "sg:person.01350253452.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350253452.46"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Genetika", 
          "id": "https://www.grid.ac/institutes/grid.418697.5", 
          "name": [
            "Stepanov Laboratory of Protein Chemistry, Institute of Genetics and Selection of Industrial Microorganisms, 1-yi Dorozhnyi Proezd 1, 117545, Moscow, Russia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ivanova", 
        "givenName": "N. M.", 
        "id": "sg:person.01171655057.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171655057.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Genetika", 
          "id": "https://www.grid.ac/institutes/grid.418697.5", 
          "name": [
            "Stepanov Laboratory of Protein Chemistry, Institute of Genetics and Selection of Industrial Microorganisms, 1-yi Dorozhnyi Proezd 1, 117545, Moscow, Russia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chestukhina", 
        "givenName": "G. G.", 
        "id": "sg:person.076337663.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.076337663.93"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1093/nar/16.15.7351", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002118380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1992.tb17184.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002170258"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.25.15804", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003882141"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0263-7855(96)00018-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005898005"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(91)81107-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009372765"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(92)90581-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011951571"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01025034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012337655", 
          "https://doi.org/10.1007/bf01025034"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m300803200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018442096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m011457200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026856923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.1993.1412", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027398230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.2004.04113.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031870891"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/328496a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033580843", 
          "https://doi.org/10.1038/328496a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pep.2004.10.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038648271"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444993007267", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045038817"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/protein/11.12.1229", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047747438"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/elps.1150181505", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049714182"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/elps.1150181505", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049714182"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.1995.0420", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050417714"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(92)90562-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051193976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/314235a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053524488", 
          "https://doi.org/10.1038/314235a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ar00158a003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055149358"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00195a017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055164734"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0501941", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055200199"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0501941", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055200199"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1546324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062490331"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075270560", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1991.tb07914.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077981954"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082475243", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2007-04", 
    "datePublishedReg": "2007-04-01", 
    "description": "Site-directed mutagenesis in the active site of Thermoactinomyces vulgaris carboxypeptidase T (CpT), which is capable of hydrolyzing both hydrophobic and positively charged substrates, resulted in five mutants: CpT1 (A243G), CpT2 (D253G/T255D), CpT3 (A243G/D253G/T255D), CpT4 (G207S/A243G/D253G/T255D), and CpT5 (G207S/A243G/T250A/D253G/T255D). These mutants step-by-step reconstruct the primary specificity pocket of carboxypeptidase B (CpB), which is capable of cleaving only positively charged C-terminal residues. All of the mutants retained the substrate specificity of the wild-type CpT. Based on comparison of three-dimensional structures of CpB and the CpT5 model, it was suggested that the lower affinity of CpT5 for positively charged substrates than the affinity of CpB could be caused by differences in nature and spatial location of Leu247 and Ile247 and of His68 and Asp65 residues in CpT and CpB, respectively, and also in location of the water molecule bound with Ala250. An additional hydrophobic region was detected in the CpT active site formed by Tyr248, Leu247, Leu203, Ala243, CH3-group of Thr250, and CO-groups of Tyr248 and Ala243, which could be responsible for binding hydrophobic substrates. Thus, notwithstanding the considerable structural similarity of CpT and pancreatic carboxypeptidases, the mechanisms underlying their substrate specificities are different.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1134/s0006297907040086", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1297322", 
        "issn": [
          "0006-2979", 
          "1608-3040"
        ], 
        "name": "Biochemistry (Moscow)", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "72"
      }
    ], 
    "name": "Structural principles of the wide substrate specificity of Thermoactinomyces vulgaris carboxypeptidase T. reconstruction of the carboxypeptidase B primary specificity pocket", 
    "pagination": "416-423", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "e90602a665820215fa8509c65a6a61363a6defa67219925a10e98658de6af6e8"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "17511606"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0376536"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1134/s0006297907040086"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1048915459"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1134/s0006297907040086", 
      "https://app.dimensions.ai/details/publication/pub.1048915459"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:56", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000537.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1134%2FS0006297907040086"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1134/s0006297907040086'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1134/s0006297907040086'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1134/s0006297907040086'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1134/s0006297907040086'


 

This table displays all metadata directly associated to this object as RDF triples.

215 TRIPLES      21 PREDICATES      65 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1134/s0006297907040086 schema:about N2bfefd4c972543d1a52a20cc7328f76d
2 N2cfdd33f8bd34b0f97a961b54360ec82
3 N3087df9223914b258dc7115410b08eb5
4 N32003eb9fbc148e396b32eff64ddfc03
5 N33b80cb6a7ea46a5bcb3a2aa62cdfa46
6 N666c2797be814c059ed83bc45a3f8f21
7 N6a51ada4c1cd46c38daf470b87f6bd8a
8 Nb9bb128ecc72473698079091940792af
9 anzsrc-for:06
10 anzsrc-for:0601
11 schema:author N9c9391e367e947b9a6ec5d6ce7c9000c
12 schema:citation sg:pub.10.1007/bf01025034
13 sg:pub.10.1038/227680a0
14 sg:pub.10.1038/314235a0
15 sg:pub.10.1038/328496a0
16 https://app.dimensions.ai/details/publication/pub.1075270560
17 https://app.dimensions.ai/details/publication/pub.1082475243
18 https://doi.org/10.1002/elps.1150181505
19 https://doi.org/10.1002/j.1460-2075.1991.tb07914.x
20 https://doi.org/10.1006/jmbi.1993.1412
21 https://doi.org/10.1006/jmbi.1995.0420
22 https://doi.org/10.1016/0003-2697(76)90527-3
23 https://doi.org/10.1016/0014-5793(91)81107-j
24 https://doi.org/10.1016/0022-2836(92)90562-x
25 https://doi.org/10.1016/0022-2836(92)90581-4
26 https://doi.org/10.1016/0263-7855(96)00018-5
27 https://doi.org/10.1016/j.pep.2004.10.020
28 https://doi.org/10.1021/ar00158a003
29 https://doi.org/10.1021/bi00195a017
30 https://doi.org/10.1021/bi0501941
31 https://doi.org/10.1074/jbc.272.25.15804
32 https://doi.org/10.1074/jbc.m011457200
33 https://doi.org/10.1074/jbc.m300803200
34 https://doi.org/10.1093/nar/16.15.7351
35 https://doi.org/10.1093/protein/11.12.1229
36 https://doi.org/10.1107/s0907444993007267
37 https://doi.org/10.1111/j.1432-1033.1992.tb17184.x
38 https://doi.org/10.1111/j.1432-1033.2004.04113.x
39 https://doi.org/10.1126/science.1546324
40 schema:datePublished 2007-04
41 schema:datePublishedReg 2007-04-01
42 schema:description Site-directed mutagenesis in the active site of Thermoactinomyces vulgaris carboxypeptidase T (CpT), which is capable of hydrolyzing both hydrophobic and positively charged substrates, resulted in five mutants: CpT1 (A243G), CpT2 (D253G/T255D), CpT3 (A243G/D253G/T255D), CpT4 (G207S/A243G/D253G/T255D), and CpT5 (G207S/A243G/T250A/D253G/T255D). These mutants step-by-step reconstruct the primary specificity pocket of carboxypeptidase B (CpB), which is capable of cleaving only positively charged C-terminal residues. All of the mutants retained the substrate specificity of the wild-type CpT. Based on comparison of three-dimensional structures of CpB and the CpT5 model, it was suggested that the lower affinity of CpT5 for positively charged substrates than the affinity of CpB could be caused by differences in nature and spatial location of Leu247 and Ile247 and of His68 and Asp65 residues in CpT and CpB, respectively, and also in location of the water molecule bound with Ala250. An additional hydrophobic region was detected in the CpT active site formed by Tyr248, Leu247, Leu203, Ala243, CH3-group of Thr250, and CO-groups of Tyr248 and Ala243, which could be responsible for binding hydrophobic substrates. Thus, notwithstanding the considerable structural similarity of CpT and pancreatic carboxypeptidases, the mechanisms underlying their substrate specificities are different.
43 schema:genre research_article
44 schema:inLanguage en
45 schema:isAccessibleForFree false
46 schema:isPartOf Nf24708f4de6945babaf9437d2243ff61
47 Nf6079294b20547c696c9e4ce5945b02b
48 sg:journal.1297322
49 schema:name Structural principles of the wide substrate specificity of Thermoactinomyces vulgaris carboxypeptidase T. reconstruction of the carboxypeptidase B primary specificity pocket
50 schema:pagination 416-423
51 schema:productId N293669a0fedc44b2bc3056fa8f307808
52 Nae02de1e039a433f87265c84e12e5cfa
53 Nb9e856d195854bcbb338b7fc7b558f6c
54 Nf053e920674a4871b1f3398fbaad33fd
55 Nf4695892e2b341e099506ed744c91cc4
56 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048915459
57 https://doi.org/10.1134/s0006297907040086
58 schema:sdDatePublished 2019-04-10T15:56
59 schema:sdLicense https://scigraph.springernature.com/explorer/license/
60 schema:sdPublisher Naae44dc9a240447eb1e2bc15f962330e
61 schema:url http://link.springer.com/10.1134%2FS0006297907040086
62 sgo:license sg:explorer/license/
63 sgo:sdDataset articles
64 rdf:type schema:ScholarlyArticle
65 N293669a0fedc44b2bc3056fa8f307808 schema:name dimensions_id
66 schema:value pub.1048915459
67 rdf:type schema:PropertyValue
68 N2bfefd4c972543d1a52a20cc7328f76d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Carboxypeptidase B
70 rdf:type schema:DefinedTerm
71 N2cfdd33f8bd34b0f97a961b54360ec82 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Bacterial Proteins
73 rdf:type schema:DefinedTerm
74 N3087df9223914b258dc7115410b08eb5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Amino Acid Substitution
76 rdf:type schema:DefinedTerm
77 N32003eb9fbc148e396b32eff64ddfc03 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name Hydrophobic and Hydrophilic Interactions
79 rdf:type schema:DefinedTerm
80 N33b80cb6a7ea46a5bcb3a2aa62cdfa46 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Models, Molecular
82 rdf:type schema:DefinedTerm
83 N420b2c98f65648c89045b2ce5e6470ae rdf:first sg:person.01225521163.11
84 rdf:rest Na50af4aaf0fd44679321e67773136b8c
85 N666c2797be814c059ed83bc45a3f8f21 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Carboxypeptidases
87 rdf:type schema:DefinedTerm
88 N6a51ada4c1cd46c38daf470b87f6bd8a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Substrate Specificity
90 rdf:type schema:DefinedTerm
91 N9c9391e367e947b9a6ec5d6ce7c9000c rdf:first sg:person.01302405151.95
92 rdf:rest N420b2c98f65648c89045b2ce5e6470ae
93 Na50af4aaf0fd44679321e67773136b8c rdf:first sg:person.01350253452.46
94 rdf:rest Nc03c5ba26838430896efba393ef6006b
95 Naae44dc9a240447eb1e2bc15f962330e schema:name Springer Nature - SN SciGraph project
96 rdf:type schema:Organization
97 Nae02de1e039a433f87265c84e12e5cfa schema:name nlm_unique_id
98 schema:value 0376536
99 rdf:type schema:PropertyValue
100 Nb9bb128ecc72473698079091940792af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Micromonosporaceae
102 rdf:type schema:DefinedTerm
103 Nb9e856d195854bcbb338b7fc7b558f6c schema:name doi
104 schema:value 10.1134/s0006297907040086
105 rdf:type schema:PropertyValue
106 Nc03c5ba26838430896efba393ef6006b rdf:first sg:person.01171655057.09
107 rdf:rest Ncd65bc027b3743ec96153ad2efcd2731
108 Ncd65bc027b3743ec96153ad2efcd2731 rdf:first sg:person.076337663.93
109 rdf:rest rdf:nil
110 Nf053e920674a4871b1f3398fbaad33fd schema:name pubmed_id
111 schema:value 17511606
112 rdf:type schema:PropertyValue
113 Nf24708f4de6945babaf9437d2243ff61 schema:issueNumber 4
114 rdf:type schema:PublicationIssue
115 Nf4695892e2b341e099506ed744c91cc4 schema:name readcube_id
116 schema:value e90602a665820215fa8509c65a6a61363a6defa67219925a10e98658de6af6e8
117 rdf:type schema:PropertyValue
118 Nf6079294b20547c696c9e4ce5945b02b schema:volumeNumber 72
119 rdf:type schema:PublicationVolume
120 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
121 schema:name Biological Sciences
122 rdf:type schema:DefinedTerm
123 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
124 schema:name Biochemistry and Cell Biology
125 rdf:type schema:DefinedTerm
126 sg:journal.1297322 schema:issn 0006-2979
127 1608-3040
128 schema:name Biochemistry (Moscow)
129 rdf:type schema:Periodical
130 sg:person.01171655057.09 schema:affiliation https://www.grid.ac/institutes/grid.418697.5
131 schema:familyName Ivanova
132 schema:givenName N. M.
133 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171655057.09
134 rdf:type schema:Person
135 sg:person.01225521163.11 schema:affiliation https://www.grid.ac/institutes/grid.418697.5
136 schema:familyName Grishin
137 schema:givenName A. M.
138 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01225521163.11
139 rdf:type schema:Person
140 sg:person.01302405151.95 schema:affiliation https://www.grid.ac/institutes/grid.418697.5
141 schema:familyName Akparov
142 schema:givenName V. Kh.
143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01302405151.95
144 rdf:type schema:Person
145 sg:person.01350253452.46 schema:affiliation https://www.grid.ac/institutes/grid.418697.5
146 schema:familyName Yusupova
147 schema:givenName M. P.
148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350253452.46
149 rdf:type schema:Person
150 sg:person.076337663.93 schema:affiliation https://www.grid.ac/institutes/grid.418697.5
151 schema:familyName Chestukhina
152 schema:givenName G. G.
153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.076337663.93
154 rdf:type schema:Person
155 sg:pub.10.1007/bf01025034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012337655
156 https://doi.org/10.1007/bf01025034
157 rdf:type schema:CreativeWork
158 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
159 https://doi.org/10.1038/227680a0
160 rdf:type schema:CreativeWork
161 sg:pub.10.1038/314235a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053524488
162 https://doi.org/10.1038/314235a0
163 rdf:type schema:CreativeWork
164 sg:pub.10.1038/328496a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033580843
165 https://doi.org/10.1038/328496a0
166 rdf:type schema:CreativeWork
167 https://app.dimensions.ai/details/publication/pub.1075270560 schema:CreativeWork
168 https://app.dimensions.ai/details/publication/pub.1082475243 schema:CreativeWork
169 https://doi.org/10.1002/elps.1150181505 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049714182
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1002/j.1460-2075.1991.tb07914.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077981954
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1006/jmbi.1993.1412 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027398230
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1006/jmbi.1995.0420 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050417714
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1016/0014-5793(91)81107-j schema:sameAs https://app.dimensions.ai/details/publication/pub.1009372765
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1016/0022-2836(92)90562-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1051193976
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1016/0022-2836(92)90581-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011951571
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/0263-7855(96)00018-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005898005
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/j.pep.2004.10.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038648271
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1021/ar00158a003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055149358
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1021/bi00195a017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055164734
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1021/bi0501941 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055200199
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1074/jbc.272.25.15804 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003882141
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1074/jbc.m011457200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026856923
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1074/jbc.m300803200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018442096
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1093/nar/16.15.7351 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002118380
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1093/protein/11.12.1229 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047747438
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1107/s0907444993007267 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045038817
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1111/j.1432-1033.1992.tb17184.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1002170258
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1111/j.1432-1033.2004.04113.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1031870891
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1126/science.1546324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062490331
212 rdf:type schema:CreativeWork
213 https://www.grid.ac/institutes/grid.418697.5 schema:alternateName Genetika
214 schema:name Stepanov Laboratory of Protein Chemistry, Institute of Genetics and Selection of Industrial Microorganisms, 1-yi Dorozhnyi Proezd 1, 117545, Moscow, Russia
215 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...