Cloning, purification and characterization of a cellulase-free xylanase from Geobacillus thermodenitrificans AK53 View Full Text


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Article Info

DATE

2016-05-19

AUTHORS

M. Irfan, H. I. Guler, A. O. Belduz, A. A. Shah, S. Canakci

ABSTRACT

Geobacillus thermodenitrificans AK53 xyl gene encoding xylanase was isolated, cloned and expressed in Escherichia coli. After purifying recombinant xylanase from G. thermodenitrificans AK53 (GthAK53Xyl) to homogeneity by ammonium sulfate precipitation and ion exchange chromatography, biochemical properties of the enzyme were determined. The kinetic studies for GthAK53Xyl showed KM value to be 4.34 mg/mL (for D-xylose) and Vmax value to be 2028.9 μmoles mg–1 min–1. The optimal temperature and pH for enzyme activity were found out to be 70°C and 5.0, respectively. The expressed protein showed the highest sequence similarity with the xylanases of G. thermodenitrificans JK1 (JN209933) and G. thermodenitrificans T-2 (EU599644). Metal cations Mg2+ and Mn2+ were found to be required for the enzyme activity, however, Co2+, Hg2+, Fe2+ and Cu2+ ions caused inhibitor effect on it. GthAK53Xyl had no cellulolytic activity and degraded xylan in an endo-fashion. The action of the enzyme on xylan from oat spelt produced xylobiose and xylopentose. The reported results are suggestive of a xylanase exhibiting desirable kinetics, stability parameters and metal resistance required for the efficient production of xylobiose at industrial scale. More... »

PAGES

277-286

References to SciGraph publications

  • 2006-04-11. Characterization of a recombinant thermostable xylanase from deep-sea thermophilic Geobacillus sp. MT-1 in East Pacific in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2012-07. Characterization of the novel xylanase from the thermophilic Geobacillus thermodenitrificans JK1 in MICROBIOLOGY
  • 1992-07. Xylan structure, microbial xylanases, and their mode of action in WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
  • 2009-03-19. A novel endo-glucanase from the thermophilic bacterium Geobacillus sp. 70PC53 with high activity and stability over a broad range of temperatures in EXTREMOPHILES
  • 2013-03-12. Thermostable and Alkalistable Endoxylanase of the Extremely Thermophilic Bacterium Geobacillus thermodenitrificans TSAA1: Cloning, Expression, Characteristics and Its Applicability in Generating Xylooligosaccharides and Fermentable Sugars in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2008-07-25. Secretome analysis of Phanerochaete chrysosporium strain CIRM-BRFM41 grown on softwood in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2006-09-13. Alkali-thermostable and cellulase-free xylanase production by an extreme thermophile Geobacillus thermoleovorans in WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
  • 2001-08. Microbial xylanases and their industrial applications: a review in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2006-11-18. Molecular cloning and heterologous expression of a new xylanase gene from Plectosphaerella cucumerina in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1970-08. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 in NATURE
  • 2000-07. Isolation and characterization of Pichia stipitis mutants with enhanced xylanase activity in WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
  • 2005-01-27. Xylanases from fungi: properties and industrial applications in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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