Production of modified magnetosome membrane proteins and analysis of their activity View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-05

AUTHORS

D. S. Grouzdev, M. V. Dziuba, A. S. Gerasimov, B. B. Kuznetsov

ABSTRACT

Genetic constructs coding for the fusion of the Mam12 membrane protein from the magnetotactic bacteria Magnetospirilium magnetotacticum with one B-domain (mb) or two B or Z domains (mbb and mzz, respectively) of Staphylococcus aureus protein A have been synthesized. Expression, fractionation, and purification of fusion proteins have been performed, and the activity of the modified proteins has been characterized. ELISA results showed that all modified proteins displayed immunoglobulin-binding activity. More... »

PAGES

220-226

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s0003683813030095

DOI

http://dx.doi.org/10.1134/s0003683813030095

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027198460


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