Transformation of Δ4-3-ketosteroids by free and immobilized cells of Rhodococcus erythropolis actinobacterium View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-07-05

AUTHORS

N. V. Carpova-Rodina, V. A. Andryushina, V. V. Yaderetz, A. V. Druzhinina, T. S. Stytsenko, B. L. Shaskol’skiy, V. I. Lozinsky, Luu Duc Huy, N. E. Voishvillo

ABSTRACT

Abstarct9α-Hydroxy derivatives were prepared from 11 steroids of androstane and pregnane series using Rhodococcus erythropolis VKPM Ac-1740 culture with 0.5–10 g/l substrate concentration in the reaction mixture. 9α-Monohydroxylation proceeded regardless of the substituent structure at C17. However, the structure of the steroid molecule influenced the time of complete conversion of the substrate and the yield of the transformation product. 9α-Hydroxy-androstenedione was obtained in 35 h in a yield of 85% when the maximum concentration of androstenedione (AD) was 10 g/l. 9α-Hydroxy-AD was also formed by the actinobacterium cells entrapped in poly(vinyl alcohol) cryogel beads. Nine successive transformation cycles were carried out using immobilized cells at 4.0 g/l concentration of AD in the medium. The yield of 9α-hydroxy-AD formed during six cycles (from two to eight with the duration of each cycle for 22–24 h) was 98%. More... »

PAGES

386-392

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s0003683811040041

DOI

http://dx.doi.org/10.1134/s0003683811040041

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021880499


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