Strain improvement for enhanced production of cellulase in Trichoderma viride View Full Text


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Article Info

DATE

2011-01

AUTHORS

F. Xu, J. Wang, S. Chen, W. Qin, Z. Yu, H. Zhao, X. Xing, H. Li

ABSTRACT

The filamentous fungi Trichoderma species produce extracellular cellulase. The current study was carried out to obtain an industrial strain with hyperproduction of cellulase. The wild-type strain, Trichoderma viride TL-124, was subjected to successive mutagenic treatments with UV irradiation, low-energy ion beam implantation, atmospheric pressure non-equilibrium discharge plasma (APNEDP), and N-methyl-N′-nitro-N-nitrosoguanidine to generate about 3000 mutants. Among these mutants, T. viride N879 strain exhibited the greatest relevant activity: 2.38-fold filter paper activity and 2.61-fold carboxymethyl cellulase, 2.18-fold β-glucosidase, and 2.27-fold cellobiohydrolase activities, compared with the respective wild-type activities, under solid-state fermentation using the inexpensive raw material wheat straw as a substrate. This work represents the first application of APNEDP in eukaryotic microorganisms. More... »

PAGES

53-58

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s0003683811010212

DOI

http://dx.doi.org/10.1134/s0003683811010212

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036387782


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