7α-Hydroxylation of steroid 5-olefins by mold fungi View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-01-06

AUTHORS

V. A. Andryushina, A. V. Druzhinina, V. V. Yaderets, T. S. Stytsenko, N. E. Voishvillo

ABSTRACT

Hydroxylation activity of the mold fungi belonging to the orders Dothideales, Hypocreales, and Mucorales towards Δ5-3β-hydroxysteroids was studied. The fungi Bipolaris sorokiniana, Fusarium sp., and Rhizopus nigricans were able to introduce hydroxy group at position 7α; however, this ability was detected only at a low substrate load and with a low yield. A 7α-hydroxylase activity of the Curvularia lunata VKPM F-981 culture was shown for the first time. It was demonstrated that the studied strain was capable of stereo- and regioselective transformations of androstane 5-olefins at a load not less than 2 g/1. Conversion of pregnane steroids by this culture yielded both 7α- and 11β-hydroxy derivatives. The introduction of 7α-hydroxy group by this strain occurred concurrently with enzymatic hydrolysis of ester groups, which proceeded under mild conditions to give the corresponding alcohols in the cases of both 3-acetate of Δ5-androstenes and mono- and triacetates of Δ5-pregnenes. More... »

PAGES

69-74

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s0003683810010126

DOI

http://dx.doi.org/10.1134/s0003683810010126

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014734113


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