The introduction of the 9α-hydroxy group into androst-4-en-3,17-dione using a new actinobacterium strain View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-07-10

AUTHORS

N. V. Rodina, V. A. Andryushina, T. S. Stytsenko, T. P. Turova, R. V. Baslerov, A. N. Panteleeva, N. E. Voishvillo

ABSTRACT

A 9α-hydrolase activity of a new actinobacterium strain identified as Rhodococcus erythropolis based on the analysis of a 16S rRNA gene sequence (1417 nucleotides) towards androst-4-en-3,17-dione (AD) was studied. In the presence of glucose in the medium, this strain completely transformed AD (4–20 g/l) into 9α-hydroxy-AD over 20–48 h. This culture was able to grow and perform AD 9α-hydroxylation at a concentration of dimethyl formamide up to 9%. Crystalline 9α-hydroxy-AD was isolated with a yield of over 90%. More... »

PAGES

395-400

References to SciGraph publications

  • 1994-04. Microorganisms as reagents for transformations of 5α-steroids in RUSSIAN CHEMICAL BULLETIN
  • 1983-07. 9α-Hydroxylation of 4-androstene-3,17-dione by gel-entrapped Corynebacterium sp. cells in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2007-02. Transformation of steroids by actinobacteria: A review in APPLIED BIOCHEMISTRY AND MICROBIOLOGY
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    http://scigraph.springernature.com/pub.10.1134/s0003683809040085

    DOI

    http://dx.doi.org/10.1134/s0003683809040085

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