The use of enzymatic preparation for the production of low molecular-weight chitosan from the king crab hepatopancrease View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-07-10

AUTHORS

A. V. Ilyina, D. S. Zagorskaya, A. N. Levov, A. I. Albulov, N. P. Kovacheva, V. P. Varlamov

ABSTRACT

This article describes the optimal conditions for the enzymatic hydrolysis of chitosan and its chemically-modified derivatives using the preparation extracted from the king crab hepatopancrease possessing pronounced hydrolythic activity. The following preparations were used: chitosan with a molecular weight of 700 kDa and an acetylation level of 0.15, carboxymethyl chitosan 200 kDa witih an extent of replacement of 0.23, and N-succinyl chitosan 390 kDa with an extent of replacement of 0.8. Low molecular-weight samples of chitosan and of its modified derivatives were obtained with the yields of 85, 55, and 80%, respectively. The conditions of the hydrolysis were as follows: an enzyme: substrate ratio of 1: 200, 37°C, and 20 h duration of hydrolysis. More... »

PAGES

374-379

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s0003683809040048

DOI

http://dx.doi.org/10.1134/s0003683809040048

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024130947


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