Promotional mechanism of high glycerol productivity in the aerobic batch fermentation of Candida glycerinogenes after feeding several amino acids View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-05

AUTHORS

T. Xie, H. Y. Fang, B. Zhuge, J. Zhuge

ABSTRACT

To disclose the addition of some strong promotional amino acids (namely glycine, glutamate, lysine and aspartic acid) is how to improve the glycerol productivity of Candida glycerinogenes. An amino acid addition strategy based on dynamic enzyme activity was applied to improve glycerol productivity and decrease the byproducts formation in a fermentation of C. glycerinogenes in a 7-1 bioreactor. Compared with the control, after feeding glycine, glutamate, lysine and aspartic acid, glycerol productivity obtained an increase of 22.3, 25.6, 23.5 and 28.6%, respectively; meanwhile, the amounts of ethanol, acetic acid and pyruvate decreased largely. Whichever glycine, lysine, glutamate or aspartic acid was fed could elevate the activities of glucose-6-phosphate dehydrogenase (G6PDH), citrate synthase (CIT), triosephosphate isomerase (TPI) and cytoplasmic NAD+AEAAKw-dependent glycerol-3-phosphate dehydrogenase (ctGPD), and reduce the activities of pyruvate kinase (PYK), phosphofructokinase (PFK) and alcohol dehydrogenase (ADH). The reason of glycerol overproduction by the yeasts after feeding glycine, glutamate, lysine or aspartic acid is that the anaplerosis of intermediate metabolites in TCA cycle for amino acid degradation can decrease the flux from Embden-Meyerhof-Parnas (EMP) pathway to TCA cycle and enhance the flux through glycerol biosynthesis pathway. Above all, not only the high active hexose monophosphate (HMP) pathway but also the high dihydroxyacetone phosphate (DHAP) level plays an important role in the high glycerol productivity of C. glycerinogenes. The strategy of amino acid supplement is significant and can be economically implemented by an online process control strategy for higher yield of glycerol in industrial scale. More... »

PAGES

303-308

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s0003683809030119

DOI

http://dx.doi.org/10.1134/s0003683809030119

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1025444846


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Hunan Institute of Engineering", 
          "id": "https://www.grid.ac/institutes/grid.459468.2", 
          "name": [
            "Department of Chemical Engineering, Hunan institute of Engineering, Donghu Road no. 18, 411104, Xiangtan, Hunan Province, China", 
            "School of Biotechnology, Southern Yangtz University, Huihe Road no. 170, 214036, Wuxi, Jiangsu Province, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xie", 
        "givenName": "T.", 
        "id": "sg:person.01234767617.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01234767617.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "School of Biotechnology, Southern Yangtz University, Huihe Road no. 170, 214036, Wuxi, Jiangsu Province, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fang", 
        "givenName": "H. Y.", 
        "id": "sg:person.010214417532.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010214417532.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "School of Biotechnology, Southern Yangtz University, Huihe Road no. 170, 214036, Wuxi, Jiangsu Province, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhuge", 
        "givenName": "B.", 
        "id": "sg:person.01363472303.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363472303.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "School of Biotechnology, Southern Yangtz University, Huihe Road no. 170, 214036, Wuxi, Jiangsu Province, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhuge", 
        "givenName": "J.", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1128/aem.68.6.2814-2821.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001270145"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002530100596", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007745540", 
          "https://doi.org/10.1007/s002530100596"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0141-0229(00)00164-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007902623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0141-0229(00)00164-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007902623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/10242429809003617", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010769063"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(99)01456-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013240658"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1139/m57-061", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014807654"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1022349401575", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016308123", 
          "https://doi.org/10.1023/a:1022349401575"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bp960043c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027718662"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1096-4959(03)00166-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041087594"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1096-4959(03)00166-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041087594"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1020451523561", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049862164", 
          "https://doi.org/10.1023/a:1020451523561"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075225857", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077214551", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009-05", 
    "datePublishedReg": "2009-05-01", 
    "description": "To disclose the addition of some strong promotional amino acids (namely glycine, glutamate, lysine and aspartic acid) is how to improve the glycerol productivity of Candida glycerinogenes. An amino acid addition strategy based on dynamic enzyme activity was applied to improve glycerol productivity and decrease the byproducts formation in a fermentation of C. glycerinogenes in a 7-1 bioreactor. Compared with the control, after feeding glycine, glutamate, lysine and aspartic acid, glycerol productivity obtained an increase of 22.3, 25.6, 23.5 and 28.6%, respectively; meanwhile, the amounts of ethanol, acetic acid and pyruvate decreased largely. Whichever glycine, lysine, glutamate or aspartic acid was fed could elevate the activities of glucose-6-phosphate dehydrogenase (G6PDH), citrate synthase (CIT), triosephosphate isomerase (TPI) and cytoplasmic NAD+AEAAKw-dependent glycerol-3-phosphate dehydrogenase (ctGPD), and reduce the activities of pyruvate kinase (PYK), phosphofructokinase (PFK) and alcohol dehydrogenase (ADH). The reason of glycerol overproduction by the yeasts after feeding glycine, glutamate, lysine or aspartic acid is that the anaplerosis of intermediate metabolites in TCA cycle for amino acid degradation can decrease the flux from Embden-Meyerhof-Parnas (EMP) pathway to TCA cycle and enhance the flux through glycerol biosynthesis pathway. Above all, not only the high active hexose monophosphate (HMP) pathway but also the high dihydroxyacetone phosphate (DHAP) level plays an important role in the high glycerol productivity of C. glycerinogenes. The strategy of amino acid supplement is significant and can be economically implemented by an online process control strategy for higher yield of glycerol in industrial scale.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1134/s0003683809030119", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1285764", 
        "issn": [
          "0555-1099", 
          "1608-3024"
        ], 
        "name": "Applied Biochemistry and Microbiology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "45"
      }
    ], 
    "name": "Promotional mechanism of high glycerol productivity in the aerobic batch fermentation of Candida glycerinogenes after feeding several amino acids", 
    "pagination": "303-308", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "7938d3b9e38f589cb5a2d7a9dbf2f82dbce973d79f58ab9116236089127ec059"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1134/s0003683809030119"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1025444846"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1134/s0003683809030119", 
      "https://app.dimensions.ai/details/publication/pub.1025444846"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:13", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000338_0000000338/records_47991_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1134/S0003683809030119"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1134/s0003683809030119'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1134/s0003683809030119'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1134/s0003683809030119'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1134/s0003683809030119'


 

This table displays all metadata directly associated to this object as RDF triples.

128 TRIPLES      21 PREDICATES      40 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1134/s0003683809030119 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author Nda4c5b0c61f94b548c0e669f52009526
4 schema:citation sg:pub.10.1007/s002530100596
5 sg:pub.10.1023/a:1020451523561
6 sg:pub.10.1023/a:1022349401575
7 https://app.dimensions.ai/details/publication/pub.1075225857
8 https://app.dimensions.ai/details/publication/pub.1077214551
9 https://doi.org/10.1016/0003-2697(76)90527-3
10 https://doi.org/10.1016/s0014-5793(99)01456-8
11 https://doi.org/10.1016/s0141-0229(00)00164-2
12 https://doi.org/10.1016/s1096-4959(03)00166-0
13 https://doi.org/10.1021/bp960043c
14 https://doi.org/10.1128/aem.68.6.2814-2821.2002
15 https://doi.org/10.1139/m57-061
16 https://doi.org/10.3109/10242429809003617
17 schema:datePublished 2009-05
18 schema:datePublishedReg 2009-05-01
19 schema:description To disclose the addition of some strong promotional amino acids (namely glycine, glutamate, lysine and aspartic acid) is how to improve the glycerol productivity of Candida glycerinogenes. An amino acid addition strategy based on dynamic enzyme activity was applied to improve glycerol productivity and decrease the byproducts formation in a fermentation of C. glycerinogenes in a 7-1 bioreactor. Compared with the control, after feeding glycine, glutamate, lysine and aspartic acid, glycerol productivity obtained an increase of 22.3, 25.6, 23.5 and 28.6%, respectively; meanwhile, the amounts of ethanol, acetic acid and pyruvate decreased largely. Whichever glycine, lysine, glutamate or aspartic acid was fed could elevate the activities of glucose-6-phosphate dehydrogenase (G6PDH), citrate synthase (CIT), triosephosphate isomerase (TPI) and cytoplasmic NAD+AEAAKw-dependent glycerol-3-phosphate dehydrogenase (ctGPD), and reduce the activities of pyruvate kinase (PYK), phosphofructokinase (PFK) and alcohol dehydrogenase (ADH). The reason of glycerol overproduction by the yeasts after feeding glycine, glutamate, lysine or aspartic acid is that the anaplerosis of intermediate metabolites in TCA cycle for amino acid degradation can decrease the flux from Embden-Meyerhof-Parnas (EMP) pathway to TCA cycle and enhance the flux through glycerol biosynthesis pathway. Above all, not only the high active hexose monophosphate (HMP) pathway but also the high dihydroxyacetone phosphate (DHAP) level plays an important role in the high glycerol productivity of C. glycerinogenes. The strategy of amino acid supplement is significant and can be economically implemented by an online process control strategy for higher yield of glycerol in industrial scale.
20 schema:genre research_article
21 schema:inLanguage en
22 schema:isAccessibleForFree false
23 schema:isPartOf N56f2101f593c477fab34a0f9a10f1e46
24 N8eb3d06da44146a587e96871985066c9
25 sg:journal.1285764
26 schema:name Promotional mechanism of high glycerol productivity in the aerobic batch fermentation of Candida glycerinogenes after feeding several amino acids
27 schema:pagination 303-308
28 schema:productId N38049b638e58446a8f4f6392b24eb31f
29 Nb74c3fec33f84d58948afabb3040600a
30 Nddbbfb4d72344721aa91cc7ae155415a
31 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025444846
32 https://doi.org/10.1134/s0003683809030119
33 schema:sdDatePublished 2019-04-11T09:13
34 schema:sdLicense https://scigraph.springernature.com/explorer/license/
35 schema:sdPublisher Nd404de0ffabb4015854358b74320d76a
36 schema:url http://link.springer.com/10.1134/S0003683809030119
37 sgo:license sg:explorer/license/
38 sgo:sdDataset articles
39 rdf:type schema:ScholarlyArticle
40 N38049b638e58446a8f4f6392b24eb31f schema:name dimensions_id
41 schema:value pub.1025444846
42 rdf:type schema:PropertyValue
43 N56f2101f593c477fab34a0f9a10f1e46 schema:issueNumber 3
44 rdf:type schema:PublicationIssue
45 N8eb3d06da44146a587e96871985066c9 schema:volumeNumber 45
46 rdf:type schema:PublicationVolume
47 N8fba6b115cba4e5ca9ac884a23d09d5f rdf:first sg:person.010214417532.67
48 rdf:rest Na1b5c2e2326446f3a02b3376d5714350
49 N988791ac01f44e4293eefd06da5cbf8e schema:affiliation Ndc05a12627424460a81998cb568dad9d
50 schema:familyName Zhuge
51 schema:givenName J.
52 rdf:type schema:Person
53 Na1b5c2e2326446f3a02b3376d5714350 rdf:first sg:person.01363472303.13
54 rdf:rest Ncff99508b3da46c5ab36166683ae5aee
55 Nb74c3fec33f84d58948afabb3040600a schema:name readcube_id
56 schema:value 7938d3b9e38f589cb5a2d7a9dbf2f82dbce973d79f58ab9116236089127ec059
57 rdf:type schema:PropertyValue
58 Ncff99508b3da46c5ab36166683ae5aee rdf:first N988791ac01f44e4293eefd06da5cbf8e
59 rdf:rest rdf:nil
60 Nd1a39d8bfdca4c539740448e5ddc37a1 schema:name School of Biotechnology, Southern Yangtz University, Huihe Road no. 170, 214036, Wuxi, Jiangsu Province, China
61 rdf:type schema:Organization
62 Nd404de0ffabb4015854358b74320d76a schema:name Springer Nature - SN SciGraph project
63 rdf:type schema:Organization
64 Nda4c5b0c61f94b548c0e669f52009526 rdf:first sg:person.01234767617.36
65 rdf:rest N8fba6b115cba4e5ca9ac884a23d09d5f
66 Ndc05a12627424460a81998cb568dad9d schema:name School of Biotechnology, Southern Yangtz University, Huihe Road no. 170, 214036, Wuxi, Jiangsu Province, China
67 rdf:type schema:Organization
68 Nddbbfb4d72344721aa91cc7ae155415a schema:name doi
69 schema:value 10.1134/s0003683809030119
70 rdf:type schema:PropertyValue
71 Nfe77afbd1fd840a89867e77d5a108225 schema:name School of Biotechnology, Southern Yangtz University, Huihe Road no. 170, 214036, Wuxi, Jiangsu Province, China
72 rdf:type schema:Organization
73 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
74 schema:name Biological Sciences
75 rdf:type schema:DefinedTerm
76 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
77 schema:name Biochemistry and Cell Biology
78 rdf:type schema:DefinedTerm
79 sg:journal.1285764 schema:issn 0555-1099
80 1608-3024
81 schema:name Applied Biochemistry and Microbiology
82 rdf:type schema:Periodical
83 sg:person.010214417532.67 schema:affiliation Nd1a39d8bfdca4c539740448e5ddc37a1
84 schema:familyName Fang
85 schema:givenName H. Y.
86 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010214417532.67
87 rdf:type schema:Person
88 sg:person.01234767617.36 schema:affiliation https://www.grid.ac/institutes/grid.459468.2
89 schema:familyName Xie
90 schema:givenName T.
91 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01234767617.36
92 rdf:type schema:Person
93 sg:person.01363472303.13 schema:affiliation Nfe77afbd1fd840a89867e77d5a108225
94 schema:familyName Zhuge
95 schema:givenName B.
96 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363472303.13
97 rdf:type schema:Person
98 sg:pub.10.1007/s002530100596 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007745540
99 https://doi.org/10.1007/s002530100596
100 rdf:type schema:CreativeWork
101 sg:pub.10.1023/a:1020451523561 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049862164
102 https://doi.org/10.1023/a:1020451523561
103 rdf:type schema:CreativeWork
104 sg:pub.10.1023/a:1022349401575 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016308123
105 https://doi.org/10.1023/a:1022349401575
106 rdf:type schema:CreativeWork
107 https://app.dimensions.ai/details/publication/pub.1075225857 schema:CreativeWork
108 https://app.dimensions.ai/details/publication/pub.1077214551 schema:CreativeWork
109 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
110 rdf:type schema:CreativeWork
111 https://doi.org/10.1016/s0014-5793(99)01456-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013240658
112 rdf:type schema:CreativeWork
113 https://doi.org/10.1016/s0141-0229(00)00164-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007902623
114 rdf:type schema:CreativeWork
115 https://doi.org/10.1016/s1096-4959(03)00166-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041087594
116 rdf:type schema:CreativeWork
117 https://doi.org/10.1021/bp960043c schema:sameAs https://app.dimensions.ai/details/publication/pub.1027718662
118 rdf:type schema:CreativeWork
119 https://doi.org/10.1128/aem.68.6.2814-2821.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001270145
120 rdf:type schema:CreativeWork
121 https://doi.org/10.1139/m57-061 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014807654
122 rdf:type schema:CreativeWork
123 https://doi.org/10.3109/10242429809003617 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010769063
124 rdf:type schema:CreativeWork
125 https://www.grid.ac/institutes/grid.459468.2 schema:alternateName Hunan Institute of Engineering
126 schema:name Department of Chemical Engineering, Hunan institute of Engineering, Donghu Road no. 18, 411104, Xiangtan, Hunan Province, China
127 School of Biotechnology, Southern Yangtz University, Huihe Road no. 170, 214036, Wuxi, Jiangsu Province, China
128 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...