A study of steroid hydroxylation activity of Curvularia lunata mycelium View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-11

AUTHORS

V. V. Yaderets, V. A. Andryushina, Yu. E. Bartoshevich, A. G. Domracheva, M. I. Novak, T. S. Stytsenko, N. E. Voishvillo

ABSTRACT

It has been demonstrated that the mycelium of Curvularia lunata at the end of the logarithmic growth phase displays a maximal 11β-hydroxylase activity towards cortexolone (4–6 g/l) used for transformation as a microcrystalline suspension in phosphate buffer. The mycelium at a later stage of fungal growth displays an elevated 14α-hydroxylase activity, necessary for generation of 14α-hydroxyandrostenedione. The effects of different forms of substrate added to the reaction mixture, age and concentration of mycelium, and fungal clones tolerant to salts of heavy metals (0.35–0.5%) were studied to remove the side 14α-hydroxylation, accompanying the main cortexolone transformation. Mycelia of the fungal clones tolerant to Co2+ and Cu2+ displayed a weak hydroxylase activity or its complete absence and an elevated content of melanin, the biosynthesis of which is intensified under adverse conditions. The results obtained suggest that the transformation of steroids by the studied C. lunata strain is a detoxication of foreign compounds. More... »

PAGES

620-624

Identifiers

URI

http://scigraph.springernature.com/pub.10.1134/s0003683807060099

DOI

http://dx.doi.org/10.1134/s0003683807060099

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024344789


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