Aberrant DNA Methylation in Non-Neoplastic Gastric Mucosa of H. Pylori Infected Patients and Effect of Eradication View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-05-17

AUTHORS

Francesco Perri, Rosa Cotugno, Ada Piepoli, Antonio Merla, Michele Quitadamo, Annamaria Gentile, Alberto Pilotto, Vito Annese, Angelo Andriulli

ABSTRACT

BACKGROUND: Gene promoter methylation is an epigenetic event leading to gene silencing. This mechanism is particularly relevant in cancer since it can interfere with the activity of specific "suppressor" genes. AIM: To evaluate promoter methylation of CDH1, p16, APC, MLH1, and COX2 in patients with H. pylori (Hp) infection before and after eradication. METHODS: Fifty-seven dyspeptic outpatients who had never performed previous endoscopy or Hp testing and treatment underwent clinical interview, endoscopy with three paired gastric biopsy specimens from the antrum, angulus, and corpus, and (13)C-urea breath test (UBT). Biopsies were scored for the presence of Hp and intestinal metaplasia (IM). DNA methylation of five tumor-related genes (CDH1, p16, MLH1, APC, and COX2) was evaluated by methylation-specific PCR in each biopsy. Infected patients were given a standard eradicating treatment and, after 1 yr, underwent endoscopy with biopsies and UBT. RESULTS: Hp infection was found in 45 patients. IM was detected in 17 out of 45 (38%) infected patients. Mean number of methylated genes was 0, 1.1 +/- 0.9, and 1.6 +/- 0.9 among the 12 Hp-/IM-, the 28 Hp+/IM-, and the 17 Hp+/IM+ patients, respectively (P < 0.0001). Specifically, promoter hypermethylation of CDH1, p16, APC, MLH1, and COX2 was found in 68%, 25%, 7%, 0%, and 14% of Hp+/IM- patients and in 71%, 29%, 35%, 12%, and 12% of Hp+/IM+ patients. No significant difference was found among the three groups of patients as far as age, smoking, alcohol, meat and vegetable consumption, and family history of gastric cancer were considered. Twenty-three out of 45 (51%) infected patients underwent the 1-yr follow-up endoscopy: 17 out of 23 (74%) were successfully eradicated. After Hp eradication, CDH1, p16, and APC methylation significantly decreased while COX2 methylation completely disappeared. Conversely, MLH1 methylation did not change significantly in patients with IM. CONCLUSION: Hp infection is associated with promoter methylation of genes which are relevant in the initiation and progression of gastric carcinogenesis. While CDH1 methylation seems to be an early event in Hp gastritis, MLH1 methylation occurs late along with IM. Hp eradication is able to significantly reduce gene methylation thus delaying or reversing Hp-induced gastric carcinogenesis. More... »

PAGES

ajg2007274

Identifiers

URI

http://scigraph.springernature.com/pub.10.1111/j.1572-0241.2007.01284.x

DOI

http://dx.doi.org/10.1111/j.1572-0241.2007.01284.x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007347811

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17509026


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adaptor Proteins, Signal Transducing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adult", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aged", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Anti-Bacterial Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, CD", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biopsy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cadherins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cyclooxygenase 2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Methylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Repair", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endoscopy, Gastrointestinal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Follow-Up Studies", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gastric Mucosa", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gastritis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Silencing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, APC", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Tumor Suppressor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, p16", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Helicobacter Infections", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Helicobacter pylori", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Middle Aged", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "MutL Protein Homolog 1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nuclear Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy", 
          "id": "http://www.grid.ac/institutes/grid.413503.0", 
          "name": [
            "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Perri", 
        "givenName": "Francesco", 
        "id": "sg:person.01056040335.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01056040335.70"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy", 
          "id": "http://www.grid.ac/institutes/grid.413503.0", 
          "name": [
            "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cotugno", 
        "givenName": "Rosa", 
        "id": "sg:person.01124153535.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01124153535.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy", 
          "id": "http://www.grid.ac/institutes/grid.413503.0", 
          "name": [
            "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Piepoli", 
        "givenName": "Ada", 
        "id": "sg:person.01140417466.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140417466.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy", 
          "id": "http://www.grid.ac/institutes/grid.413503.0", 
          "name": [
            "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Merla", 
        "givenName": "Antonio", 
        "id": "sg:person.0645773626.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645773626.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy", 
          "id": "http://www.grid.ac/institutes/grid.413503.0", 
          "name": [
            "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Quitadamo", 
        "givenName": "Michele", 
        "id": "sg:person.0745231700.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0745231700.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy", 
          "id": "http://www.grid.ac/institutes/grid.413503.0", 
          "name": [
            "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gentile", 
        "givenName": "Annamaria", 
        "id": "sg:person.01372330747.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01372330747.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departments of Geriatrics, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy", 
          "id": "http://www.grid.ac/institutes/grid.413503.0", 
          "name": [
            "Departments of Geriatrics, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pilotto", 
        "givenName": "Alberto", 
        "id": "sg:person.01002377430.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002377430.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy", 
          "id": "http://www.grid.ac/institutes/grid.413503.0", 
          "name": [
            "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Annese", 
        "givenName": "Vito", 
        "id": "sg:person.0655214656.73", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0655214656.73"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy", 
          "id": "http://www.grid.ac/institutes/grid.413503.0", 
          "name": [
            "Departments of Gastroenterology, \u201cCasa Sollievo della Sofferenza\u201d Hospital, IRCCS, San Giovanni Rotondo, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Andriulli", 
        "givenName": "Angelo", 
        "id": "sg:person.0731565272.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0731565272.40"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1054/bjoc.2001.1888", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001758867", 
          "https://doi.org/10.1054/bjoc.2001.1888"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1203704", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049710108", 
          "https://doi.org/10.1038/sj.onc.1203704"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg1748", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006797620", 
          "https://doi.org/10.1038/nrg1748"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.3780422", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016963046", 
          "https://doi.org/10.1038/labinvest.3780422"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1097/01.lab.0000064704.53132.65", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043417850", 
          "https://doi.org/10.1097/01.lab.0000064704.53132.65"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bjc.6601600", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044192483", 
          "https://doi.org/10.1038/sj.bjc.6601600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/32918", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044664817", 
          "https://doi.org/10.1038/32918"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35006081", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030124366", 
          "https://doi.org/10.1038/35006081"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00535-006-1846-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027560954", 
          "https://doi.org/10.1007/s00535-006-1846-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bjc.6600319", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050650257", 
          "https://doi.org/10.1038/sj.bjc.6600319"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2007-05-17", 
    "datePublishedReg": "2007-05-17", 
    "description": "BACKGROUND: Gene promoter methylation is an epigenetic event leading to gene silencing. This mechanism is particularly relevant in cancer since it can interfere with the activity of specific \"suppressor\" genes.\nAIM: To evaluate promoter methylation of CDH1, p16, APC, MLH1, and COX2 in patients with H. pylori (Hp) infection before and after eradication.\nMETHODS: Fifty-seven dyspeptic outpatients who had never performed previous endoscopy or Hp testing and treatment underwent clinical interview, endoscopy with three paired gastric biopsy specimens from the antrum, angulus, and corpus, and (13)C-urea breath test (UBT). Biopsies were scored for the presence of Hp and intestinal metaplasia (IM). DNA methylation of five tumor-related genes (CDH1, p16, MLH1, APC, and COX2) was evaluated by methylation-specific PCR in each biopsy. Infected patients were given a standard eradicating treatment and, after 1 yr, underwent endoscopy with biopsies and UBT.\nRESULTS: Hp infection was found in 45 patients. IM was detected in 17 out of 45 (38%) infected patients. Mean number of methylated genes was 0, 1.1 +/- 0.9, and 1.6 +/- 0.9 among the 12 Hp-/IM-, the 28 Hp+/IM-, and the 17 Hp+/IM+ patients, respectively (P < 0.0001). Specifically, promoter hypermethylation of CDH1, p16, APC, MLH1, and COX2 was found in 68%, 25%, 7%, 0%, and 14% of Hp+/IM- patients and in 71%, 29%, 35%, 12%, and 12% of Hp+/IM+ patients. No significant difference was found among the three groups of patients as far as age, smoking, alcohol, meat and vegetable consumption, and family history of gastric cancer were considered. Twenty-three out of 45 (51%) infected patients underwent the 1-yr follow-up endoscopy: 17 out of 23 (74%) were successfully eradicated. After Hp eradication, CDH1, p16, and APC methylation significantly decreased while COX2 methylation completely disappeared. Conversely, MLH1 methylation did not change significantly in patients with IM.\nCONCLUSION: Hp infection is associated with promoter methylation of genes which are relevant in the initiation and progression of gastric carcinogenesis. While CDH1 methylation seems to be an early event in Hp gastritis, MLH1 methylation occurs late along with IM. Hp eradication is able to significantly reduce gene methylation thus delaying or reversing Hp-induced gastric carcinogenesis.", 
    "genre": "article", 
    "id": "sg:pub.10.1111/j.1572-0241.2007.01284.x", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1019809", 
        "issn": [
          "0002-9270", 
          "1572-0241"
        ], 
        "name": "The American Journal of Gastroenterology", 
        "publisher": "Wolters Kluwer", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "102"
      }
    ], 
    "keywords": [
      "urea breath test", 
      "intestinal metaplasia", 
      "infected patients", 
      "MLH1 methylation", 
      "promoter methylation", 
      "gastric carcinogenesis", 
      "pylori infected patients", 
      "group of patients", 
      "effect of eradication", 
      "gastric biopsy specimens", 
      "non-neoplastic gastric mucosa", 
      "Hp testing", 
      "previous endoscopy", 
      "underwent endoscopy", 
      "HP eradication", 
      "pylori infection", 
      "gene promoter methylation", 
      "HP gastritis", 
      "biopsy specimens", 
      "methylation-specific PCR", 
      "dyspeptic outpatients", 
      "family history", 
      "gastric cancer", 
      "breath test", 
      "gastric mucosa", 
      "vegetable consumption", 
      "patients", 
      "Clinical Interview", 
      "tumor-related genes", 
      "endoscopy", 
      "biopsy", 
      "infection", 
      "mean number", 
      "promoter hypermethylation", 
      "eradication", 
      "aberrant DNA methylation", 
      "significant differences", 
      "CDH1 methylation", 
      "p16", 
      "presence of HP", 
      "early events", 
      "DNA methylation", 
      "cancer", 
      "gene methylation", 
      "APC methylation", 
      "carcinogenesis", 
      "MLH1", 
      "treatment", 
      "APC", 
      "COX2", 
      "CDH1", 
      "epigenetic events", 
      "smoking", 
      "gastritis", 
      "metaplasia", 
      "outpatients", 
      "antrum", 
      "angulus", 
      "mucosa", 
      "follow", 
      "genes", 
      "progression", 
      "methylation", 
      "age", 
      "hypermethylation", 
      "events", 
      "PCR", 
      "group", 
      "suppressor", 
      "initiation", 
      "differences", 
      "yr", 
      "testing", 
      "activity", 
      "history", 
      "specimens", 
      "meat", 
      "alcohol", 
      "interviews", 
      "test", 
      "HP", 
      "effect", 
      "presence", 
      "mechanism", 
      "number", 
      "consumption", 
      "corpus", 
      "Hp-/IM", 
      "COX2 methylation"
    ], 
    "name": "Aberrant DNA Methylation in Non-Neoplastic Gastric Mucosa of H. Pylori Infected Patients and Effect of Eradication", 
    "pagination": "ajg2007274", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1007347811"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1111/j.1572-0241.2007.01284.x"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "17509026"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1111/j.1572-0241.2007.01284.x", 
      "https://app.dimensions.ai/details/publication/pub.1007347811"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:18", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_452.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1111/j.1572-0241.2007.01284.x"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1111/j.1572-0241.2007.01284.x'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1111/j.1572-0241.2007.01284.x'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1111/j.1572-0241.2007.01284.x'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1111/j.1572-0241.2007.01284.x'


 

This table displays all metadata directly associated to this object as RDF triples.

365 TRIPLES      22 PREDICATES      154 URIs      136 LITERALS      36 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1111/j.1572-0241.2007.01284.x schema:about N00a4f106b35840f5be42d62af3d4eec8
2 N0e2cf2e6ddb84363b13eac104bc9b50f
3 N2876f8a42db74f8889ba28228631fff6
4 N3b0651326be94250bc3a032a0c8a0c40
5 N4c1abfb4237e436fbe7272abf46f8747
6 N4c488370fcad4b658a1030a8868f7471
7 N52e531767aa54a71b040bf6bc575f495
8 N5ca385f1d5624ff3bac11f728d06f720
9 N65cf3df21dcc4672ab3a3011eb61d53e
10 N68962a7d2f3c4ee4b45db7ebcc5d7698
11 N6ab4f00ebb0f41369abda9ce639ea43e
12 N6f2bd8c7fb2e45439152f043e55ca371
13 N734f63561eff454ba7b55b8f7ec85b4e
14 N8014dc5b3ff14eeda21a75272f8f5a23
15 N91884c01bd0840159e63e2e5f90d9818
16 N9725ce0f392841a092606a8601e9ad06
17 Na156a126259e46bf8caa2c758f2009a9
18 Na2995decdc334d0298cfd94f5777e59d
19 Nba612384f52c4968b65d35cf22ed726e
20 Nbef429487b85409bbf5b85dff1dbd9f5
21 Nc5c7dea006ec4b349f413693e425acc5
22 Nce5fbc88a212487c91fd4f4bdfd86b4a
23 Ncec05f8291b04db582439711ca0526e1
24 Nd271da9cd622480f809b5c64a78ca7e4
25 Nddb77aa2d0ae417db5ad34f217d2766b
26 Ne31ef815a7204b85a0ea7b8fc5ab2aa3
27 Ne7e4841a9b3e4eae826148f0c2e5a7a4
28 Ne8252bd059c14dda968c42e211e73986
29 Ne859385a140b43e6832f98b514dec87e
30 anzsrc-for:11
31 anzsrc-for:1103
32 schema:author N8122504665864777a4d3c8994d720b48
33 schema:citation sg:pub.10.1007/s00535-006-1846-6
34 sg:pub.10.1038/32918
35 sg:pub.10.1038/35006081
36 sg:pub.10.1038/labinvest.3780422
37 sg:pub.10.1038/nrg1748
38 sg:pub.10.1038/sj.bjc.6600319
39 sg:pub.10.1038/sj.bjc.6601600
40 sg:pub.10.1038/sj.onc.1203704
41 sg:pub.10.1054/bjoc.2001.1888
42 sg:pub.10.1097/01.lab.0000064704.53132.65
43 schema:datePublished 2007-05-17
44 schema:datePublishedReg 2007-05-17
45 schema:description BACKGROUND: Gene promoter methylation is an epigenetic event leading to gene silencing. This mechanism is particularly relevant in cancer since it can interfere with the activity of specific "suppressor" genes. AIM: To evaluate promoter methylation of CDH1, p16, APC, MLH1, and COX2 in patients with H. pylori (Hp) infection before and after eradication. METHODS: Fifty-seven dyspeptic outpatients who had never performed previous endoscopy or Hp testing and treatment underwent clinical interview, endoscopy with three paired gastric biopsy specimens from the antrum, angulus, and corpus, and (13)C-urea breath test (UBT). Biopsies were scored for the presence of Hp and intestinal metaplasia (IM). DNA methylation of five tumor-related genes (CDH1, p16, MLH1, APC, and COX2) was evaluated by methylation-specific PCR in each biopsy. Infected patients were given a standard eradicating treatment and, after 1 yr, underwent endoscopy with biopsies and UBT. RESULTS: Hp infection was found in 45 patients. IM was detected in 17 out of 45 (38%) infected patients. Mean number of methylated genes was 0, 1.1 +/- 0.9, and 1.6 +/- 0.9 among the 12 Hp-/IM-, the 28 Hp+/IM-, and the 17 Hp+/IM+ patients, respectively (P < 0.0001). Specifically, promoter hypermethylation of CDH1, p16, APC, MLH1, and COX2 was found in 68%, 25%, 7%, 0%, and 14% of Hp+/IM- patients and in 71%, 29%, 35%, 12%, and 12% of Hp+/IM+ patients. No significant difference was found among the three groups of patients as far as age, smoking, alcohol, meat and vegetable consumption, and family history of gastric cancer were considered. Twenty-three out of 45 (51%) infected patients underwent the 1-yr follow-up endoscopy: 17 out of 23 (74%) were successfully eradicated. After Hp eradication, CDH1, p16, and APC methylation significantly decreased while COX2 methylation completely disappeared. Conversely, MLH1 methylation did not change significantly in patients with IM. CONCLUSION: Hp infection is associated with promoter methylation of genes which are relevant in the initiation and progression of gastric carcinogenesis. While CDH1 methylation seems to be an early event in Hp gastritis, MLH1 methylation occurs late along with IM. Hp eradication is able to significantly reduce gene methylation thus delaying or reversing Hp-induced gastric carcinogenesis.
46 schema:genre article
47 schema:inLanguage en
48 schema:isAccessibleForFree false
49 schema:isPartOf N72ee89e8bdb34a38818238b371623768
50 N98f1468381a84ca09d2ffcba84dfa6a4
51 sg:journal.1019809
52 schema:keywords APC
53 APC methylation
54 CDH1
55 CDH1 methylation
56 COX2
57 COX2 methylation
58 Clinical Interview
59 DNA methylation
60 HP
61 HP eradication
62 HP gastritis
63 Hp testing
64 Hp-/IM
65 MLH1
66 MLH1 methylation
67 PCR
68 aberrant DNA methylation
69 activity
70 age
71 alcohol
72 angulus
73 antrum
74 biopsy
75 biopsy specimens
76 breath test
77 cancer
78 carcinogenesis
79 consumption
80 corpus
81 differences
82 dyspeptic outpatients
83 early events
84 effect
85 effect of eradication
86 endoscopy
87 epigenetic events
88 eradication
89 events
90 family history
91 follow
92 gastric biopsy specimens
93 gastric cancer
94 gastric carcinogenesis
95 gastric mucosa
96 gastritis
97 gene methylation
98 gene promoter methylation
99 genes
100 group
101 group of patients
102 history
103 hypermethylation
104 infected patients
105 infection
106 initiation
107 interviews
108 intestinal metaplasia
109 mean number
110 meat
111 mechanism
112 metaplasia
113 methylation
114 methylation-specific PCR
115 mucosa
116 non-neoplastic gastric mucosa
117 number
118 outpatients
119 p16
120 patients
121 presence
122 presence of HP
123 previous endoscopy
124 progression
125 promoter hypermethylation
126 promoter methylation
127 pylori infected patients
128 pylori infection
129 significant differences
130 smoking
131 specimens
132 suppressor
133 test
134 testing
135 treatment
136 tumor-related genes
137 underwent endoscopy
138 urea breath test
139 vegetable consumption
140 yr
141 schema:name Aberrant DNA Methylation in Non-Neoplastic Gastric Mucosa of H. Pylori Infected Patients and Effect of Eradication
142 schema:pagination ajg2007274
143 schema:productId N3dac050182ce4884b38f0b697c140235
144 Nac7a775d6b044bdea8fc0202d3d5fda3
145 Nea0c6da85799454cbe018b32af68510f
146 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007347811
147 https://doi.org/10.1111/j.1572-0241.2007.01284.x
148 schema:sdDatePublished 2022-01-01T18:18
149 schema:sdLicense https://scigraph.springernature.com/explorer/license/
150 schema:sdPublisher N6ea4c1644ce84e4bb92431d61a352c2d
151 schema:url https://doi.org/10.1111/j.1572-0241.2007.01284.x
152 sgo:license sg:explorer/license/
153 sgo:sdDataset articles
154 rdf:type schema:ScholarlyArticle
155 N00a4f106b35840f5be42d62af3d4eec8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Male
157 rdf:type schema:DefinedTerm
158 N02aa2e8c68434a56b48cd35428b9fd31 rdf:first sg:person.01002377430.05
159 rdf:rest Ne7a2b27b3fdb409ea439277addf31a21
160 N0e2cf2e6ddb84363b13eac104bc9b50f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Polymerase Chain Reaction
162 rdf:type schema:DefinedTerm
163 N1568b16753a34608bbd750f617c453b4 rdf:first sg:person.01140417466.74
164 rdf:rest Ne599f78e66b746aaaf31750ce754ab28
165 N2876f8a42db74f8889ba28228631fff6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Genes, Tumor Suppressor
167 rdf:type schema:DefinedTerm
168 N3444a30510c64be1bfca55bfde32075f rdf:first sg:person.0731565272.40
169 rdf:rest rdf:nil
170 N3b0651326be94250bc3a032a0c8a0c40 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name Female
172 rdf:type schema:DefinedTerm
173 N3dac050182ce4884b38f0b697c140235 schema:name doi
174 schema:value 10.1111/j.1572-0241.2007.01284.x
175 rdf:type schema:PropertyValue
176 N4c1abfb4237e436fbe7272abf46f8747 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
177 schema:name Endoscopy, Gastrointestinal
178 rdf:type schema:DefinedTerm
179 N4c488370fcad4b658a1030a8868f7471 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
180 schema:name DNA
181 rdf:type schema:DefinedTerm
182 N4d84bd231a754f94991cfadc41f0fb74 rdf:first sg:person.01372330747.77
183 rdf:rest N02aa2e8c68434a56b48cd35428b9fd31
184 N52e531767aa54a71b040bf6bc575f495 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
185 schema:name Follow-Up Studies
186 rdf:type schema:DefinedTerm
187 N5ca385f1d5624ff3bac11f728d06f720 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
188 schema:name DNA Methylation
189 rdf:type schema:DefinedTerm
190 N65cf3df21dcc4672ab3a3011eb61d53e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
191 schema:name Membrane Proteins
192 rdf:type schema:DefinedTerm
193 N68962a7d2f3c4ee4b45db7ebcc5d7698 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
194 schema:name Cyclooxygenase 2
195 rdf:type schema:DefinedTerm
196 N6ab4f00ebb0f41369abda9ce639ea43e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
197 schema:name Gene Silencing
198 rdf:type schema:DefinedTerm
199 N6ea4c1644ce84e4bb92431d61a352c2d schema:name Springer Nature - SN SciGraph project
200 rdf:type schema:Organization
201 N6f2bd8c7fb2e45439152f043e55ca371 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
202 schema:name Nuclear Proteins
203 rdf:type schema:DefinedTerm
204 N72ee89e8bdb34a38818238b371623768 schema:issueNumber 7
205 rdf:type schema:PublicationIssue
206 N734f63561eff454ba7b55b8f7ec85b4e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
207 schema:name Gastritis
208 rdf:type schema:DefinedTerm
209 N8014dc5b3ff14eeda21a75272f8f5a23 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
210 schema:name Aged
211 rdf:type schema:DefinedTerm
212 N8122504665864777a4d3c8994d720b48 rdf:first sg:person.01056040335.70
213 rdf:rest Nf1eb76c5d72648bb82595bac728b1208
214 N91884c01bd0840159e63e2e5f90d9818 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
215 schema:name Genes, APC
216 rdf:type schema:DefinedTerm
217 N9725ce0f392841a092606a8601e9ad06 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
218 schema:name Antigens, CD
219 rdf:type schema:DefinedTerm
220 N98f1468381a84ca09d2ffcba84dfa6a4 schema:volumeNumber 102
221 rdf:type schema:PublicationVolume
222 Na156a126259e46bf8caa2c758f2009a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
223 schema:name Cadherins
224 rdf:type schema:DefinedTerm
225 Na2995decdc334d0298cfd94f5777e59d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
226 schema:name Gastric Mucosa
227 rdf:type schema:DefinedTerm
228 Nac7a775d6b044bdea8fc0202d3d5fda3 schema:name pubmed_id
229 schema:value 17509026
230 rdf:type schema:PropertyValue
231 Nb44b509691a4483ba6fd8b81497796d2 rdf:first sg:person.0745231700.97
232 rdf:rest N4d84bd231a754f94991cfadc41f0fb74
233 Nba612384f52c4968b65d35cf22ed726e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
234 schema:name Helicobacter Infections
235 rdf:type schema:DefinedTerm
236 Nbef429487b85409bbf5b85dff1dbd9f5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
237 schema:name DNA Repair
238 rdf:type schema:DefinedTerm
239 Nc5c7dea006ec4b349f413693e425acc5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
240 schema:name Genes, p16
241 rdf:type schema:DefinedTerm
242 Nce5fbc88a212487c91fd4f4bdfd86b4a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
243 schema:name MutL Protein Homolog 1
244 rdf:type schema:DefinedTerm
245 Ncec05f8291b04db582439711ca0526e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
246 schema:name Anti-Bacterial Agents
247 rdf:type schema:DefinedTerm
248 Nd271da9cd622480f809b5c64a78ca7e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
249 schema:name Helicobacter pylori
250 rdf:type schema:DefinedTerm
251 Nddb77aa2d0ae417db5ad34f217d2766b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
252 schema:name Humans
253 rdf:type schema:DefinedTerm
254 Ne31ef815a7204b85a0ea7b8fc5ab2aa3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
255 schema:name Adaptor Proteins, Signal Transducing
256 rdf:type schema:DefinedTerm
257 Ne599f78e66b746aaaf31750ce754ab28 rdf:first sg:person.0645773626.19
258 rdf:rest Nb44b509691a4483ba6fd8b81497796d2
259 Ne7a2b27b3fdb409ea439277addf31a21 rdf:first sg:person.0655214656.73
260 rdf:rest N3444a30510c64be1bfca55bfde32075f
261 Ne7e4841a9b3e4eae826148f0c2e5a7a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
262 schema:name Biopsy
263 rdf:type schema:DefinedTerm
264 Ne8252bd059c14dda968c42e211e73986 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
265 schema:name Adult
266 rdf:type schema:DefinedTerm
267 Ne859385a140b43e6832f98b514dec87e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
268 schema:name Middle Aged
269 rdf:type schema:DefinedTerm
270 Nea0c6da85799454cbe018b32af68510f schema:name dimensions_id
271 schema:value pub.1007347811
272 rdf:type schema:PropertyValue
273 Nf1eb76c5d72648bb82595bac728b1208 rdf:first sg:person.01124153535.15
274 rdf:rest N1568b16753a34608bbd750f617c453b4
275 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
276 schema:name Medical and Health Sciences
277 rdf:type schema:DefinedTerm
278 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
279 schema:name Clinical Sciences
280 rdf:type schema:DefinedTerm
281 sg:journal.1019809 schema:issn 0002-9270
282 1572-0241
283 schema:name The American Journal of Gastroenterology
284 schema:publisher Wolters Kluwer
285 rdf:type schema:Periodical
286 sg:person.01002377430.05 schema:affiliation grid-institutes:grid.413503.0
287 schema:familyName Pilotto
288 schema:givenName Alberto
289 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002377430.05
290 rdf:type schema:Person
291 sg:person.01056040335.70 schema:affiliation grid-institutes:grid.413503.0
292 schema:familyName Perri
293 schema:givenName Francesco
294 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01056040335.70
295 rdf:type schema:Person
296 sg:person.01124153535.15 schema:affiliation grid-institutes:grid.413503.0
297 schema:familyName Cotugno
298 schema:givenName Rosa
299 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01124153535.15
300 rdf:type schema:Person
301 sg:person.01140417466.74 schema:affiliation grid-institutes:grid.413503.0
302 schema:familyName Piepoli
303 schema:givenName Ada
304 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140417466.74
305 rdf:type schema:Person
306 sg:person.01372330747.77 schema:affiliation grid-institutes:grid.413503.0
307 schema:familyName Gentile
308 schema:givenName Annamaria
309 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01372330747.77
310 rdf:type schema:Person
311 sg:person.0645773626.19 schema:affiliation grid-institutes:grid.413503.0
312 schema:familyName Merla
313 schema:givenName Antonio
314 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0645773626.19
315 rdf:type schema:Person
316 sg:person.0655214656.73 schema:affiliation grid-institutes:grid.413503.0
317 schema:familyName Annese
318 schema:givenName Vito
319 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0655214656.73
320 rdf:type schema:Person
321 sg:person.0731565272.40 schema:affiliation grid-institutes:grid.413503.0
322 schema:familyName Andriulli
323 schema:givenName Angelo
324 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0731565272.40
325 rdf:type schema:Person
326 sg:person.0745231700.97 schema:affiliation grid-institutes:grid.413503.0
327 schema:familyName Quitadamo
328 schema:givenName Michele
329 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0745231700.97
330 rdf:type schema:Person
331 sg:pub.10.1007/s00535-006-1846-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027560954
332 https://doi.org/10.1007/s00535-006-1846-6
333 rdf:type schema:CreativeWork
334 sg:pub.10.1038/32918 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044664817
335 https://doi.org/10.1038/32918
336 rdf:type schema:CreativeWork
337 sg:pub.10.1038/35006081 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030124366
338 https://doi.org/10.1038/35006081
339 rdf:type schema:CreativeWork
340 sg:pub.10.1038/labinvest.3780422 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016963046
341 https://doi.org/10.1038/labinvest.3780422
342 rdf:type schema:CreativeWork
343 sg:pub.10.1038/nrg1748 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006797620
344 https://doi.org/10.1038/nrg1748
345 rdf:type schema:CreativeWork
346 sg:pub.10.1038/sj.bjc.6600319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050650257
347 https://doi.org/10.1038/sj.bjc.6600319
348 rdf:type schema:CreativeWork
349 sg:pub.10.1038/sj.bjc.6601600 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044192483
350 https://doi.org/10.1038/sj.bjc.6601600
351 rdf:type schema:CreativeWork
352 sg:pub.10.1038/sj.onc.1203704 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049710108
353 https://doi.org/10.1038/sj.onc.1203704
354 rdf:type schema:CreativeWork
355 sg:pub.10.1054/bjoc.2001.1888 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001758867
356 https://doi.org/10.1054/bjoc.2001.1888
357 rdf:type schema:CreativeWork
358 sg:pub.10.1097/01.lab.0000064704.53132.65 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043417850
359 https://doi.org/10.1097/01.lab.0000064704.53132.65
360 rdf:type schema:CreativeWork
361 grid-institutes:grid.413503.0 schema:alternateName Departments of Gastroenterology, “Casa Sollievo della Sofferenza” Hospital, IRCCS, San Giovanni Rotondo, Italy
362 Departments of Geriatrics, “Casa Sollievo della Sofferenza” Hospital, IRCCS, San Giovanni Rotondo, Italy
363 schema:name Departments of Gastroenterology, “Casa Sollievo della Sofferenza” Hospital, IRCCS, San Giovanni Rotondo, Italy
364 Departments of Geriatrics, “Casa Sollievo della Sofferenza” Hospital, IRCCS, San Giovanni Rotondo, Italy
365 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...