HMEC-1: Establishment of an Immortalized Human Microvascular Endothelial Cell Line View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1992-12

AUTHORS

E W Ades, F J Candal, R A Swerlick, V G George, S Summers, D C Bosse, T J Lawley

ABSTRACT

The study of human microvascular endothelial cells has been limited, because these cells are difficult to isolate in pure culture, are fastidious in their in vitro growth requirements, and have a very limited lifespan. In order to overcome these difficulties, we have transfected human dermal microvascular endothelial cells (HMEC) with a PBR-322-based plasmid containing the coding region for the simian virus 40 A gene product, large T antigen, and succeeded in immortalizing them. These cells, termed CDC/EU.HMEC-1 (HMEC-1), have been passaged 95 times to date and show no signs of senescence, whereas normal microvascular endothelial cells undergo senescence at passages 8-10. HMEC-1 exhibit typical cobblestone morphology when grown in monolayer culture, express and secrete von Willebrand's Factor, take up acteylated low-density lipoprotein, and rapidly form tubes when cultured on matrigel. HMEC-1 grow to densities three to seven times higher than microvascular endothelial cells and require much less stringent growth medium. HMEC-1 will grow in the absence of human serum, whereas microvascular endothelial cells require culture medium supplemented with 30% human serum. These cells express other cell-surface molecules typically associated with endothelial cells, including CD31 and CD36 and epitopes identified by monoclonal antibodies EN4 and PAL-E. They also express the cell adhesion molecules ICAM-1 and CD44 and following stimulation with interferon-gamma express major histocompatibility complex class II antigens. HMEC-1 specifically bind lymphocytes in cell adhesion assays. Thus HMEC-1 is the first immortalized human microvascular endothelial cell line that retains the morphologic, phenotypic, and functional characteristics of normal human microvascular endothelial cells. More... »

PAGES

683-690

Identifiers

URI

http://scigraph.springernature.com/pub.10.1111/1523-1747.ep12613748

DOI

http://dx.doi.org/10.1111/1523-1747.ep12613748

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010462004

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1361507


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, Differentiation, Myelomonocytic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, Polyomavirus Transforming", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Adhesion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Adhesion Molecules", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endothelium, Vascular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Intercellular Adhesion Molecule-1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microcirculation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phenotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Platelet Endothelial Cell Adhesion Molecule-1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Centers for Disease Control and Prevention", 
          "id": "https://www.grid.ac/institutes/grid.416738.f", 
          "name": [
            "Biological Products Branch, Centers for Disease Control, Atlanta, Georgia."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ades", 
        "givenName": "E W", 
        "id": "sg:person.01003125537.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003125537.02"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Candal", 
        "givenName": "F J", 
        "id": "sg:person.01340740774.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01340740774.53"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Swerlick", 
        "givenName": "R A", 
        "id": "sg:person.0104127733.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0104127733.06"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "George", 
        "givenName": "V G", 
        "id": "sg:person.01140603676.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140603676.16"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Summers", 
        "givenName": "S", 
        "type": "Person"
      }, 
      {
        "familyName": "Bosse", 
        "givenName": "D C", 
        "id": "sg:person.01255032276.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01255032276.34"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Lawley", 
        "givenName": "T J", 
        "id": "sg:person.01133451070.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01133451070.97"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/jcp.1041410310", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001205193"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.107.4.1589", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005436877"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci111509", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006613254"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(92)90115-s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007440282"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-2952(87)90252-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008215567"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-2952(87)90252-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008215567"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/1523-1747.ep12530941", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008260848", 
          "https://doi.org/10.1111/1523-1747.ep12530941"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jmv.1890250212", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014084523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1041410307", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015284345"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/1523-1747.ep12479643", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017178030", 
          "https://doi.org/10.1111/1523-1747.ep12479643"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02630896", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017403836", 
          "https://doi.org/10.1007/bf02630896"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(73)90341-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017876073"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-065x.1989.tb00010.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018494995"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/349796a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024922902", 
          "https://doi.org/10.1038/349796a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1041340106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026108609"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-291x(91)91221-w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027134334"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1159/000163589", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028386568"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02618597", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029466783", 
          "https://doi.org/10.1007/bf02618597"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02618597", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029466783", 
          "https://doi.org/10.1007/bf02618597"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02624456", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030535764", 
          "https://doi.org/10.1007/bf02624456"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02624456", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030535764", 
          "https://doi.org/10.1007/bf02624456"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.109.2.927", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032831789"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.1041420314", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033267919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/346425a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035127925", 
          "https://doi.org/10.1038/346425a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/0471142735.im0703s00", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035317888"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.76.10.5217", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036710787"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/36.10.2458407", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042041505"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/36.10.2458407", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042041505"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/eji.1830220221", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042993330"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02623586", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044880678", 
          "https://doi.org/10.1007/bf02623586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02623586", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044880678", 
          "https://doi.org/10.1007/bf02623586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(76)90132-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046571961"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/288551a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048791001", 
          "https://doi.org/10.1038/288551a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci111574", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050442699"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci107470", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053383708"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1348-0421.1988.tb01364.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053700408"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/1523-1747.ep12466229", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1061837250"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2588007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062545143"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075946416", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076009629", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077586840", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077844590", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078050172", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078390126", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078408810", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078655729", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078758867", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078879213", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079433846", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079545102", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079701936", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080011649", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081840494", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081987466", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082154406", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1992-12", 
    "datePublishedReg": "1992-12-01", 
    "description": "The study of human microvascular endothelial cells has been limited, because these cells are difficult to isolate in pure culture, are fastidious in their in vitro growth requirements, and have a very limited lifespan. In order to overcome these difficulties, we have transfected human dermal microvascular endothelial cells (HMEC) with a PBR-322-based plasmid containing the coding region for the simian virus 40 A gene product, large T antigen, and succeeded in immortalizing them. These cells, termed CDC/EU.HMEC-1 (HMEC-1), have been passaged 95 times to date and show no signs of senescence, whereas normal microvascular endothelial cells undergo senescence at passages 8-10. HMEC-1 exhibit typical cobblestone morphology when grown in monolayer culture, express and secrete von Willebrand's Factor, take up acteylated low-density lipoprotein, and rapidly form tubes when cultured on matrigel. HMEC-1 grow to densities three to seven times higher than microvascular endothelial cells and require much less stringent growth medium. HMEC-1 will grow in the absence of human serum, whereas microvascular endothelial cells require culture medium supplemented with 30% human serum. These cells express other cell-surface molecules typically associated with endothelial cells, including CD31 and CD36 and epitopes identified by monoclonal antibodies EN4 and PAL-E. They also express the cell adhesion molecules ICAM-1 and CD44 and following stimulation with interferon-gamma express major histocompatibility complex class II antigens. HMEC-1 specifically bind lymphocytes in cell adhesion assays. Thus HMEC-1 is the first immortalized human microvascular endothelial cell line that retains the morphologic, phenotypic, and functional characteristics of normal human microvascular endothelial cells.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1111/1523-1747.ep12613748", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1020153", 
        "issn": [
          "0022-202X", 
          "1523-1747"
        ], 
        "name": "Journal of Investigative Dermatology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "99"
      }
    ], 
    "name": "HMEC-1: Establishment of an Immortalized Human Microvascular Endothelial Cell Line", 
    "pagination": "683-690", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "31d72d0ed2b5b8b2411a13c107649e2e51b0cdd9d63b34f0f34d8fa6d251afb7"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "1361507"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0426720"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1111/1523-1747.ep12613748"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1010462004"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1111/1523-1747.ep12613748", 
      "https://app.dimensions.ai/details/publication/pub.1010462004"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T19:19", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8678_00000582.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/jid/journal/v99/n6/full/5611816a.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12613748'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12613748'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12613748'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12613748'


 

This table displays all metadata directly associated to this object as RDF triples.

302 TRIPLES      21 PREDICATES      93 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1111/1523-1747.ep12613748 schema:about N248a050c1712401c918f0680f4186d66
2 N2780c70985bf402f9416908f948ce177
3 N29f976a3c5254ce493c9beec8489272e
4 N488b778cb31c43e08c70104effe87a65
5 N6b67ff0a2f154834ac71dd69a4ef2b8e
6 N7d78386fc3dd4c2985fac37ad1589209
7 N7e0e394b78b341e0a0854c5fecea3e80
8 N90027c31a1bc44f08b1ffd263700650d
9 N99a031ce508244479e24d36d5fcd6b85
10 Na2e661debff041d8bc04f08e6756cab0
11 Na4fbebad88664236a5ef1f819ea0f670
12 Naf42b38067554523ad95191ec27e106e
13 Neffb5b5949184f2a957c11c83bd21339
14 Nf6083e76ff4441d289c015db4feed4c5
15 anzsrc-for:11
16 anzsrc-for:1107
17 schema:author Ne5be1fd70cf44610a6b952b8602b0885
18 schema:citation sg:pub.10.1007/bf02618597
19 sg:pub.10.1007/bf02623586
20 sg:pub.10.1007/bf02624456
21 sg:pub.10.1007/bf02630896
22 sg:pub.10.1038/288551a0
23 sg:pub.10.1038/346425a0
24 sg:pub.10.1038/349796a0
25 sg:pub.10.1111/1523-1747.ep12479643
26 sg:pub.10.1111/1523-1747.ep12530941
27 https://app.dimensions.ai/details/publication/pub.1075946416
28 https://app.dimensions.ai/details/publication/pub.1076009629
29 https://app.dimensions.ai/details/publication/pub.1077586840
30 https://app.dimensions.ai/details/publication/pub.1077844590
31 https://app.dimensions.ai/details/publication/pub.1078050172
32 https://app.dimensions.ai/details/publication/pub.1078390126
33 https://app.dimensions.ai/details/publication/pub.1078408810
34 https://app.dimensions.ai/details/publication/pub.1078655729
35 https://app.dimensions.ai/details/publication/pub.1078758867
36 https://app.dimensions.ai/details/publication/pub.1078879213
37 https://app.dimensions.ai/details/publication/pub.1079433846
38 https://app.dimensions.ai/details/publication/pub.1079545102
39 https://app.dimensions.ai/details/publication/pub.1079701936
40 https://app.dimensions.ai/details/publication/pub.1080011649
41 https://app.dimensions.ai/details/publication/pub.1081840494
42 https://app.dimensions.ai/details/publication/pub.1081987466
43 https://app.dimensions.ai/details/publication/pub.1082154406
44 https://doi.org/10.1002/0471142735.im0703s00
45 https://doi.org/10.1002/eji.1830220221
46 https://doi.org/10.1002/jcp.1041340106
47 https://doi.org/10.1002/jcp.1041410307
48 https://doi.org/10.1002/jcp.1041410310
49 https://doi.org/10.1002/jcp.1041420314
50 https://doi.org/10.1002/jmv.1890250212
51 https://doi.org/10.1016/0006-291x(91)91221-w
52 https://doi.org/10.1016/0006-2952(87)90252-8
53 https://doi.org/10.1016/0042-6822(73)90341-3
54 https://doi.org/10.1016/0092-8674(76)90132-x
55 https://doi.org/10.1016/0092-8674(92)90115-s
56 https://doi.org/10.1073/pnas.76.10.5217
57 https://doi.org/10.1083/jcb.107.4.1589
58 https://doi.org/10.1083/jcb.109.2.927
59 https://doi.org/10.1111/1523-1747.ep12466229
60 https://doi.org/10.1111/j.1348-0421.1988.tb01364.x
61 https://doi.org/10.1111/j.1600-065x.1989.tb00010.x
62 https://doi.org/10.1126/science.2588007
63 https://doi.org/10.1159/000163589
64 https://doi.org/10.1172/jci107470
65 https://doi.org/10.1172/jci111509
66 https://doi.org/10.1172/jci111574
67 https://doi.org/10.1177/36.10.2458407
68 schema:datePublished 1992-12
69 schema:datePublishedReg 1992-12-01
70 schema:description The study of human microvascular endothelial cells has been limited, because these cells are difficult to isolate in pure culture, are fastidious in their in vitro growth requirements, and have a very limited lifespan. In order to overcome these difficulties, we have transfected human dermal microvascular endothelial cells (HMEC) with a PBR-322-based plasmid containing the coding region for the simian virus 40 A gene product, large T antigen, and succeeded in immortalizing them. These cells, termed CDC/EU.HMEC-1 (HMEC-1), have been passaged 95 times to date and show no signs of senescence, whereas normal microvascular endothelial cells undergo senescence at passages 8-10. HMEC-1 exhibit typical cobblestone morphology when grown in monolayer culture, express and secrete von Willebrand's Factor, take up acteylated low-density lipoprotein, and rapidly form tubes when cultured on matrigel. HMEC-1 grow to densities three to seven times higher than microvascular endothelial cells and require much less stringent growth medium. HMEC-1 will grow in the absence of human serum, whereas microvascular endothelial cells require culture medium supplemented with 30% human serum. These cells express other cell-surface molecules typically associated with endothelial cells, including CD31 and CD36 and epitopes identified by monoclonal antibodies EN4 and PAL-E. They also express the cell adhesion molecules ICAM-1 and CD44 and following stimulation with interferon-gamma express major histocompatibility complex class II antigens. HMEC-1 specifically bind lymphocytes in cell adhesion assays. Thus HMEC-1 is the first immortalized human microvascular endothelial cell line that retains the morphologic, phenotypic, and functional characteristics of normal human microvascular endothelial cells.
71 schema:genre research_article
72 schema:inLanguage en
73 schema:isAccessibleForFree true
74 schema:isPartOf Ned03e19125a04740b622dab2b10e3ba2
75 Nf56906a7bac64bf482437d14e33a4606
76 sg:journal.1020153
77 schema:name HMEC-1: Establishment of an Immortalized Human Microvascular Endothelial Cell Line
78 schema:pagination 683-690
79 schema:productId N53b0c5a94a234061a9165e8c49b441c2
80 N6c05e5c31a1f4ed5845d33a1eeba781a
81 N6e84819a23b643c3b10f052b36dfebc9
82 N81a15e0d1e334a7da67b2c0c6c8b5d39
83 Na3aa82ac49d84459b34f92a86b19850a
84 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010462004
85 https://doi.org/10.1111/1523-1747.ep12613748
86 schema:sdDatePublished 2019-04-10T19:19
87 schema:sdLicense https://scigraph.springernature.com/explorer/license/
88 schema:sdPublisher N4ce52dd1a53045f198204dd678167053
89 schema:url http://www.nature.com/jid/journal/v99/n6/full/5611816a.html
90 sgo:license sg:explorer/license/
91 sgo:sdDataset articles
92 rdf:type schema:ScholarlyArticle
93 N005da898bc8c480b82c8eee81b18a1c0 rdf:first sg:person.0104127733.06
94 rdf:rest N746584c556dc4af6805053eb68478ffc
95 N19cc1fe58a9242ee9d4c02d5f99ad997 rdf:first Nb5c4089ca20d460b8ed2c9e9af8398e5
96 rdf:rest N76fe254658cc4e518a81777b81a9c0f6
97 N248a050c1712401c918f0680f4186d66 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Cell Line
99 rdf:type schema:DefinedTerm
100 N2780c70985bf402f9416908f948ce177 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Intercellular Adhesion Molecule-1
102 rdf:type schema:DefinedTerm
103 N29f976a3c5254ce493c9beec8489272e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name T-Lymphocytes
105 rdf:type schema:DefinedTerm
106 N488b778cb31c43e08c70104effe87a65 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Phenotype
108 rdf:type schema:DefinedTerm
109 N4ce52dd1a53045f198204dd678167053 schema:name Springer Nature - SN SciGraph project
110 rdf:type schema:Organization
111 N53b0c5a94a234061a9165e8c49b441c2 schema:name doi
112 schema:value 10.1111/1523-1747.ep12613748
113 rdf:type schema:PropertyValue
114 N6b67ff0a2f154834ac71dd69a4ef2b8e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Endothelium, Vascular
116 rdf:type schema:DefinedTerm
117 N6c05e5c31a1f4ed5845d33a1eeba781a schema:name dimensions_id
118 schema:value pub.1010462004
119 rdf:type schema:PropertyValue
120 N6e84819a23b643c3b10f052b36dfebc9 schema:name readcube_id
121 schema:value 31d72d0ed2b5b8b2411a13c107649e2e51b0cdd9d63b34f0f34d8fa6d251afb7
122 rdf:type schema:PropertyValue
123 N746584c556dc4af6805053eb68478ffc rdf:first sg:person.01140603676.16
124 rdf:rest N19cc1fe58a9242ee9d4c02d5f99ad997
125 N76fe254658cc4e518a81777b81a9c0f6 rdf:first sg:person.01255032276.34
126 rdf:rest Nc018a448624941cb916a2f28fae87fbf
127 N7d78386fc3dd4c2985fac37ad1589209 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Humans
129 rdf:type schema:DefinedTerm
130 N7e0e394b78b341e0a0854c5fecea3e80 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Male
132 rdf:type schema:DefinedTerm
133 N81a15e0d1e334a7da67b2c0c6c8b5d39 schema:name nlm_unique_id
134 schema:value 0426720
135 rdf:type schema:PropertyValue
136 N8847bad4411c46bb95c1c9727e151323 rdf:first sg:person.01340740774.53
137 rdf:rest N005da898bc8c480b82c8eee81b18a1c0
138 N90027c31a1bc44f08b1ffd263700650d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Transfection
140 rdf:type schema:DefinedTerm
141 N99a031ce508244479e24d36d5fcd6b85 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Cell Adhesion
143 rdf:type schema:DefinedTerm
144 Na2e661debff041d8bc04f08e6756cab0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Cell Adhesion Molecules
146 rdf:type schema:DefinedTerm
147 Na3aa82ac49d84459b34f92a86b19850a schema:name pubmed_id
148 schema:value 1361507
149 rdf:type schema:PropertyValue
150 Na4fbebad88664236a5ef1f819ea0f670 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Platelet Endothelial Cell Adhesion Molecule-1
152 rdf:type schema:DefinedTerm
153 Naf42b38067554523ad95191ec27e106e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Microcirculation
155 rdf:type schema:DefinedTerm
156 Nb5c4089ca20d460b8ed2c9e9af8398e5 schema:familyName Summers
157 schema:givenName S
158 rdf:type schema:Person
159 Nc018a448624941cb916a2f28fae87fbf rdf:first sg:person.01133451070.97
160 rdf:rest rdf:nil
161 Ne5be1fd70cf44610a6b952b8602b0885 rdf:first sg:person.01003125537.02
162 rdf:rest N8847bad4411c46bb95c1c9727e151323
163 Ned03e19125a04740b622dab2b10e3ba2 schema:issueNumber 6
164 rdf:type schema:PublicationIssue
165 Neffb5b5949184f2a957c11c83bd21339 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Antigens, Polyomavirus Transforming
167 rdf:type schema:DefinedTerm
168 Nf56906a7bac64bf482437d14e33a4606 schema:volumeNumber 99
169 rdf:type schema:PublicationVolume
170 Nf6083e76ff4441d289c015db4feed4c5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name Antigens, Differentiation, Myelomonocytic
172 rdf:type schema:DefinedTerm
173 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
174 schema:name Medical and Health Sciences
175 rdf:type schema:DefinedTerm
176 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
177 schema:name Immunology
178 rdf:type schema:DefinedTerm
179 sg:journal.1020153 schema:issn 0022-202X
180 1523-1747
181 schema:name Journal of Investigative Dermatology
182 rdf:type schema:Periodical
183 sg:person.01003125537.02 schema:affiliation https://www.grid.ac/institutes/grid.416738.f
184 schema:familyName Ades
185 schema:givenName E W
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003125537.02
187 rdf:type schema:Person
188 sg:person.0104127733.06 schema:familyName Swerlick
189 schema:givenName R A
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0104127733.06
191 rdf:type schema:Person
192 sg:person.01133451070.97 schema:familyName Lawley
193 schema:givenName T J
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01133451070.97
195 rdf:type schema:Person
196 sg:person.01140603676.16 schema:familyName George
197 schema:givenName V G
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140603676.16
199 rdf:type schema:Person
200 sg:person.01255032276.34 schema:familyName Bosse
201 schema:givenName D C
202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01255032276.34
203 rdf:type schema:Person
204 sg:person.01340740774.53 schema:familyName Candal
205 schema:givenName F J
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01340740774.53
207 rdf:type schema:Person
208 sg:pub.10.1007/bf02618597 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029466783
209 https://doi.org/10.1007/bf02618597
210 rdf:type schema:CreativeWork
211 sg:pub.10.1007/bf02623586 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044880678
212 https://doi.org/10.1007/bf02623586
213 rdf:type schema:CreativeWork
214 sg:pub.10.1007/bf02624456 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030535764
215 https://doi.org/10.1007/bf02624456
216 rdf:type schema:CreativeWork
217 sg:pub.10.1007/bf02630896 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017403836
218 https://doi.org/10.1007/bf02630896
219 rdf:type schema:CreativeWork
220 sg:pub.10.1038/288551a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048791001
221 https://doi.org/10.1038/288551a0
222 rdf:type schema:CreativeWork
223 sg:pub.10.1038/346425a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035127925
224 https://doi.org/10.1038/346425a0
225 rdf:type schema:CreativeWork
226 sg:pub.10.1038/349796a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024922902
227 https://doi.org/10.1038/349796a0
228 rdf:type schema:CreativeWork
229 sg:pub.10.1111/1523-1747.ep12479643 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017178030
230 https://doi.org/10.1111/1523-1747.ep12479643
231 rdf:type schema:CreativeWork
232 sg:pub.10.1111/1523-1747.ep12530941 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008260848
233 https://doi.org/10.1111/1523-1747.ep12530941
234 rdf:type schema:CreativeWork
235 https://app.dimensions.ai/details/publication/pub.1075946416 schema:CreativeWork
236 https://app.dimensions.ai/details/publication/pub.1076009629 schema:CreativeWork
237 https://app.dimensions.ai/details/publication/pub.1077586840 schema:CreativeWork
238 https://app.dimensions.ai/details/publication/pub.1077844590 schema:CreativeWork
239 https://app.dimensions.ai/details/publication/pub.1078050172 schema:CreativeWork
240 https://app.dimensions.ai/details/publication/pub.1078390126 schema:CreativeWork
241 https://app.dimensions.ai/details/publication/pub.1078408810 schema:CreativeWork
242 https://app.dimensions.ai/details/publication/pub.1078655729 schema:CreativeWork
243 https://app.dimensions.ai/details/publication/pub.1078758867 schema:CreativeWork
244 https://app.dimensions.ai/details/publication/pub.1078879213 schema:CreativeWork
245 https://app.dimensions.ai/details/publication/pub.1079433846 schema:CreativeWork
246 https://app.dimensions.ai/details/publication/pub.1079545102 schema:CreativeWork
247 https://app.dimensions.ai/details/publication/pub.1079701936 schema:CreativeWork
248 https://app.dimensions.ai/details/publication/pub.1080011649 schema:CreativeWork
249 https://app.dimensions.ai/details/publication/pub.1081840494 schema:CreativeWork
250 https://app.dimensions.ai/details/publication/pub.1081987466 schema:CreativeWork
251 https://app.dimensions.ai/details/publication/pub.1082154406 schema:CreativeWork
252 https://doi.org/10.1002/0471142735.im0703s00 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035317888
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1002/eji.1830220221 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042993330
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1002/jcp.1041340106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026108609
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1002/jcp.1041410307 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015284345
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1002/jcp.1041410310 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001205193
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1002/jcp.1041420314 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033267919
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1002/jmv.1890250212 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014084523
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1016/0006-291x(91)91221-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1027134334
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1016/0006-2952(87)90252-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008215567
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1016/0042-6822(73)90341-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017876073
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1016/0092-8674(76)90132-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1046571961
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1016/0092-8674(92)90115-s schema:sameAs https://app.dimensions.ai/details/publication/pub.1007440282
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1073/pnas.76.10.5217 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036710787
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1083/jcb.107.4.1589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005436877
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1083/jcb.109.2.927 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032831789
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1111/1523-1747.ep12466229 schema:sameAs https://app.dimensions.ai/details/publication/pub.1061837250
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1111/j.1348-0421.1988.tb01364.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1053700408
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1111/j.1600-065x.1989.tb00010.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1018494995
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1126/science.2588007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062545143
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1159/000163589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028386568
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1172/jci107470 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053383708
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1172/jci111509 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006613254
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1172/jci111574 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050442699
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1177/36.10.2458407 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042041505
299 rdf:type schema:CreativeWork
300 https://www.grid.ac/institutes/grid.416738.f schema:alternateName Centers for Disease Control and Prevention
301 schema:name Biological Products Branch, Centers for Disease Control, Atlanta, Georgia.
302 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...