Enrichment of Epidermal Langerhans Cells: Studies with a Monolayer Technique and Flow Cytometry Sorting View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1982-06

AUTHORS

Annika Scheynius, Lars Klareskog, Urban Forsum, Per Matsson, Leif Karlsson, Per A Peterson, Christer Sundström

ABSTRACT

Langerhans cells were enriched from epidermal cell suspensions by a monolayer technique based on the association of Langerhans cells with solid matrix-bound anti-Ia antibodies or by flow cytometry sorting of fluorescein-isothiocyanate labeled anti-Ia reactive cells. The monolayer technique gave a moderate enrichment (15-37%) whereas considerably higher purity (73-87%) was obtained by flow cytometry sorting. The identity of the enriched anti-Ia reactive cells as Langerhans cells was established by histochemical techniques or electron microscopy. The monolayer-enriched Langerhans cells could function as stimulating cells in the mixed leukocyte culture reaction and as antigen-presenting cells. More... »

PAGES

452-455

Identifiers

URI

http://scigraph.springernature.com/pub.10.1111/1523-1747.ep12510125

DOI

http://dx.doi.org/10.1111/1523-1747.ep12510125

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011398969

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6211496


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