Serial Cultivation of Single Keratinocytes from the Outer Root Sheath of Human Scalp Hair Follicles View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1986-10

AUTHORS

Alain Limat, Friedrich K Noser

ABSTRACT

A method for the isolation of outer root sheath keratinocytes from plucked human hair follicles and for their subsequent cultivation has been developed. The selective trypsinization of outer root sheath keratinocytes provided a single cell suspension of defined origin within the hair follicle. The 3T3 feeder layer technique supports sustained growth of these cells in that as little as one single plucked hair follicle (yielding approximately 1.5 X 10(4) cells) consistently gave rise to a confluent 35-mm culture dish (with approximately 1.5 X 10(6) cells) within about 2 weeks. The outer root sheath keratinocytes can be serially passaged for up to 3 times and also cryopreserved. More... »

PAGES

485-488

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1111/1523-1747.ep12455548

DOI

http://dx.doi.org/10.1111/1523-1747.ep12455548

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031594384

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3531357


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