Topography of Mammalian Connexins in Human Skin View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1994-08

AUTHORS

D Salomon, E Masgrau, S Vischer, S Ullrich, E Dupont, P Sappino, J H Saurat, P Meda

ABSTRACT

We have explored the expression of gap junction proteins in normal human skin by immunostaining cryostat sections (indirect immunofluorescence) or lyophilized epidermis (Western blotting) with antibodies against four mammalian connexins Cx26, Cx32, Cx40, Cx43; and by hybridizing total epidermal RNA (Northern blotting) with cRNA probes for Cx26, Cx32, and Cx43. We found that epidermal keratinocytes express Cx43 but not Cx26, Cx32, or Cx40. This expression was minimal in the basal layer, much higher in the spinous layer, reduced in the granular layer, and absent in the stratum corneum. Immunostaining for Cx43 was also observed in sebaceous glands, hairs, and eccrine sweat ducts. The two latter epidermal adnexae were also markedly labeled by antibodies against Cx26, a gap junction protein that was undetectable by immunofluorescence in interfollicular keratinocytes. Immunoblots of polyacrylamide gel electrophoresis-separated epidermal proteins and hybridization of epidermal RNA confirmed the presence of Cx43 in epidermis. These observations indicate that 1) Cx43 and Cx26 are components of human keratinocyte gap junctions; 2) these two proteins are differentially expressed in the interfollicular epidermis and the skin adnexae; 3) in interfollicular epidermis, Cx43 is a predominant gap junction protein, mostly expressed by the differentiating spinous cells; 4) Cx43 distribution is in accordance with the extensive dye coupling previously observed in this epidermal compartment. More... »

PAGES

240-247

Identifiers

URI

http://scigraph.springernature.com/pub.10.1111/1523-1747.ep12393218

DOI

http://dx.doi.org/10.1111/1523-1747.ep12393218

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038363245

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7518858


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12393218'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12393218'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12393218'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12393218'


 

This table displays all metadata directly associated to this object as RDF triples.

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309 schema:name Clinique de Dermatologie, Hôpital Cantonal Universitaire, Genève, Switzerland.
310 rdf:type schema:Organization
 




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