Expression of B7 Costimulatory Molecule in Cultured Human Epidermal Langerhans Cells Is Regulated at the mRNA Level View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1994-07

AUTHORS

G Girolomoni, G Zambruno, R Manfredini, V Zacchi, S Ferrari, A Cossarizza, A Giannetti

ABSTRACT

Langerhans cells (LC) belong to the dendritic cell lineage and are the principal antigen-presenting cells of squamous epithelia. Short-term cultured LC (cLC) exhibit a marked augmented capacity to stimulate allogeneic T cells and acquire the ability to activate naive T cells, probably in relation to enhanced expression of accessory signals. In this study, we evaluated the expression of B7 costimulatory molecule (CD80) in human freshly isolated (fLC) and cLC at both the protein and mRNA level. Staining of frozen skin sections did not reveal any epidermal dendritic cell reactive with either of two different anti-B7 monoclonal antibodies. fLC in suspension did not exhibit any B7 staining as evaluated by two-color flow-cytometry analysis and immunoelectron microscopy. In contrast, LC that were cultured for 24-72 h displayed strong surface B7 reactivity with a characteristic patchy pattern. Treatment with dispase and trypsin did not reduce B7 staining of cLC. Following warming to 37 degrees C, cLC tagged with anti-B7 monoclonal antibody and gold-conjugated secondary antibody could internalize surface B7 by using the organelles of receptor-mediated endocytosis. B7 mRNA, detected by the reverse-transcriptase polymerase chain reaction technique, was expressed at a low level in purified (> 90% HLA-DR+) fLC but not in LC-depleted epidermal cells, and was markedly upregulated in purified cLC. The results indicate that 1) fLC do not express B7 protein on their surface, but acquire B7 during culture, 2) surface B7 is not sensitive to trypsin, 3) B7 expression is regulated primarily at the mRNA level, and 4) membrane B7 can be internalized within cLC. B7 molecule on CLC may be relevant to their increased antigen-presenting cell potency and ability to stimulate naive T lymphocytes. More... »

PAGES

54-59

References to SciGraph publications

  • 1993-03. Phorbol-12-Myristate-13-Acetate-Treated Human Keratinocytes Express B7-Like Molecules That Serve a Costimulatory Role in T-Cell Activation in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1992-06. Ultraviolet B-Irradiated Antigen-Presenting Cells Display Altered Accessory Signaling for T-Cell Activation: Relevance to Immune Responses Initiated in Skin in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1992-10. Internalization of Ia Molecules into Birbeck Granule-Like Structures in Murine Dendritic Cells in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1993-01. Adhesion of epidermal Langerhans cells to keratinocytes mediated by E-cadherin in NATURE
  • 1993-03. Epidermal Langerhans Cells Are Resistant to the Permeabilizing Effects of Extracellular ATP: In Vitro Evidence Supporting a Protective Role of Membrane ATPase in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1989-11. Cultured Human Langerhans Cells Resemble Lymphoid Dendritic Cells in Phenotype and Function in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1992-07. The immunologic properties of epidermal Langerhans cells as a part of the dendritic cell system in SEMINARS IN IMMUNOPATHOLOGY
  • 1992-09. Cultured Human Langerhans Cells Process and Present Intact Protein Antigens in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1990-06. Internalization and Acidification of Surface HLA-DR Molecules by Epidermal Langerhans Cells: A Paradigm for Antigen Processing in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1993-02. Up-Regulation of α4 Integrin on Activated Langerhans Cells: Analysis of Adhesion Molecules on Langerhans Cells Relating to Their Migration from Skin to Draining Lymph Nodes in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1111/1523-1747.ep12389619

    DOI

    http://dx.doi.org/10.1111/1523-1747.ep12389619

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1048964060

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/7517982


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Immunology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Antibodies, Monoclonal", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "B7-1 Antigen", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Cultured", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Endocytosis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Flow Cytometry", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fluorescent Antibody Technique", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Regulation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "In Situ Hybridization", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Langerhans Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Microscopy, Immunoelectron", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymerase Chain Reaction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA, Messenger", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Up-Regulation", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Modena and Reggio Emilia", 
              "id": "https://www.grid.ac/institutes/grid.7548.e", 
              "name": [
                "Department of Dermatology, University of Modena, Italy."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Girolomoni", 
            "givenName": "G", 
            "id": "sg:person.01252556266.57", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01252556266.57"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Zambruno", 
            "givenName": "G", 
            "id": "sg:person.01315030632.54", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315030632.54"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Manfredini", 
            "givenName": "R", 
            "id": "sg:person.01153321671.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153321671.40"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Zacchi", 
            "givenName": "V", 
            "id": "sg:person.01126305436.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01126305436.02"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Ferrari", 
            "givenName": "S", 
            "id": "sg:person.016710673632.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016710673632.01"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Cossarizza", 
            "givenName": "A", 
            "id": "sg:person.01220110375.49", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220110375.49"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Giannetti", 
            "givenName": "A", 
            "id": "sg:person.01167611267.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167611267.01"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1073/pnas.84.9.2901", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004375900"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1111/1523-1747.ep12319727", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005997357", 
              "https://doi.org/10.1111/1523-1747.ep12319727"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1172/jci115840", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006005619"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1111/1523-1747.ep12874611", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006497117", 
              "https://doi.org/10.1111/1523-1747.ep12874611"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1111/1523-1747.ep12462236", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007703237", 
              "https://doi.org/10.1111/1523-1747.ep12462236"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/eji.1830221207", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007991747"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1084/jem.178.6.1893", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011998739"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1111/1523-1747.ep12616663", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012666866", 
              "https://doi.org/10.1111/1523-1747.ep12616663"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/12.3.1687", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012769682"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1111/1523-1747.ep12616079", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016849724", 
              "https://doi.org/10.1111/1523-1747.ep12616079"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1084/jem.177.1.165", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019400364"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1111/1523-1747.ep12462783", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020896416", 
              "https://doi.org/10.1111/1523-1747.ep12462783"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1084/jem.176.4.1215", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022004115"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1111/1523-1747.ep12469748", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022935908", 
              "https://doi.org/10.1111/1523-1747.ep12469748"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1600-0625.1992.tb00004.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027251796"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00200527", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027657509", 
              "https://doi.org/10.1007/bf00200527"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00200527", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027657509", 
              "https://doi.org/10.1007/bf00200527"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0952-7915(92)90075-p", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030628918"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0952-7915(92)90075-p", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030628918"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1111/1523-1747.ep12469769", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030955715", 
              "https://doi.org/10.1111/1523-1747.ep12469769"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/361082a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032702155", 
              "https://doi.org/10.1038/361082a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1084/jem.175.2.437", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049243831"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1084/jem.175.5.1353", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050670867"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1084/jem.178.2.381", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052243592"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/intimm/5.3.317", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059711896"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.2113314", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062522797"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.7678351", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062648365"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075302032", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075960660", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1076028307", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077604686", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079368590", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079522118", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079834682", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082462857", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082463094", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082463473", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082798254", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082813577", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082826102", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1994-07", 
        "datePublishedReg": "1994-07-01", 
        "description": "Langerhans cells (LC) belong to the dendritic cell lineage and are the principal antigen-presenting cells of squamous epithelia. Short-term cultured LC (cLC) exhibit a marked augmented capacity to stimulate allogeneic T cells and acquire the ability to activate naive T cells, probably in relation to enhanced expression of accessory signals. In this study, we evaluated the expression of B7 costimulatory molecule (CD80) in human freshly isolated (fLC) and cLC at both the protein and mRNA level. Staining of frozen skin sections did not reveal any epidermal dendritic cell reactive with either of two different anti-B7 monoclonal antibodies. fLC in suspension did not exhibit any B7 staining as evaluated by two-color flow-cytometry analysis and immunoelectron microscopy. In contrast, LC that were cultured for 24-72 h displayed strong surface B7 reactivity with a characteristic patchy pattern. Treatment with dispase and trypsin did not reduce B7 staining of cLC. Following warming to 37 degrees C, cLC tagged with anti-B7 monoclonal antibody and gold-conjugated secondary antibody could internalize surface B7 by using the organelles of receptor-mediated endocytosis. B7 mRNA, detected by the reverse-transcriptase polymerase chain reaction technique, was expressed at a low level in purified (> 90% HLA-DR+) fLC but not in LC-depleted epidermal cells, and was markedly upregulated in purified cLC. The results indicate that 1) fLC do not express B7 protein on their surface, but acquire B7 during culture, 2) surface B7 is not sensitive to trypsin, 3) B7 expression is regulated primarily at the mRNA level, and 4) membrane B7 can be internalized within cLC. B7 molecule on CLC may be relevant to their increased antigen-presenting cell potency and ability to stimulate naive T lymphocytes.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1111/1523-1747.ep12389619", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1020153", 
            "issn": [
              "0022-202X", 
              "1523-1747"
            ], 
            "name": "Journal of Investigative Dermatology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "103"
          }
        ], 
        "name": "Expression of B7 Costimulatory Molecule in Cultured Human Epidermal Langerhans Cells Is Regulated at the mRNA Level", 
        "pagination": "54-59", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "e941ea5ca57e26e202d7722c4713fa2bccd05e01d7a66372a80c43af78fa3930"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "7517982"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0426720"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1111/1523-1747.ep12389619"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1048964060"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1111/1523-1747.ep12389619", 
          "https://app.dimensions.ai/details/publication/pub.1048964060"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T19:45", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8681_00000426.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/jid/journal/v103/n1/full/5611188a.html"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12389619'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12389619'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12389619'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12389619'


     

    This table displays all metadata directly associated to this object as RDF triples.

    280 TRIPLES      21 PREDICATES      83 URIs      37 LITERALS      25 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1111/1523-1747.ep12389619 schema:about N05c178578673476d8bf9247ffe899a9d
    2 N106def0c30544b86b502b6ce91f52c19
    3 N30b9c3c5145c4b6c8900ba3d3a0bbc4f
    4 N31c347caf6c94444a4b7b556a3a55f9e
    5 N367d4c3c77194927b73a918d8572f485
    6 N648ac2a03583413f989651a95276bb6d
    7 N74c05d07407d49b2b38f3e73af490191
    8 N853c081d546f4d0a866e1a82fda47512
    9 N94b094d702314875904db0228916b9e6
    10 Nb79650db94394777a0233959f2abc828
    11 Nc4ba0bbf78ec4310b164ab27362bd972
    12 Nc74b32b5552c45b49de80386ea9cb424
    13 Nc9a4fd10c9cb4237899980cf01d96e9c
    14 Nd330c25949864e4c8048216e74a0e8cd
    15 Nec0e4cbe6dcd4413a03cc27481e08aee
    16 Nf329a0eb975d4bc98e128fe550e1758f
    17 anzsrc-for:11
    18 anzsrc-for:1107
    19 schema:author N16346b24e5454539b17c58da54ed81e2
    20 schema:citation sg:pub.10.1007/bf00200527
    21 sg:pub.10.1038/361082a0
    22 sg:pub.10.1111/1523-1747.ep12319727
    23 sg:pub.10.1111/1523-1747.ep12462236
    24 sg:pub.10.1111/1523-1747.ep12462783
    25 sg:pub.10.1111/1523-1747.ep12469748
    26 sg:pub.10.1111/1523-1747.ep12469769
    27 sg:pub.10.1111/1523-1747.ep12616079
    28 sg:pub.10.1111/1523-1747.ep12616663
    29 sg:pub.10.1111/1523-1747.ep12874611
    30 https://app.dimensions.ai/details/publication/pub.1075302032
    31 https://app.dimensions.ai/details/publication/pub.1075960660
    32 https://app.dimensions.ai/details/publication/pub.1076028307
    33 https://app.dimensions.ai/details/publication/pub.1077604686
    34 https://app.dimensions.ai/details/publication/pub.1079368590
    35 https://app.dimensions.ai/details/publication/pub.1079522118
    36 https://app.dimensions.ai/details/publication/pub.1079834682
    37 https://app.dimensions.ai/details/publication/pub.1082462857
    38 https://app.dimensions.ai/details/publication/pub.1082463094
    39 https://app.dimensions.ai/details/publication/pub.1082463473
    40 https://app.dimensions.ai/details/publication/pub.1082798254
    41 https://app.dimensions.ai/details/publication/pub.1082813577
    42 https://app.dimensions.ai/details/publication/pub.1082826102
    43 https://doi.org/10.1002/eji.1830221207
    44 https://doi.org/10.1016/0952-7915(92)90075-p
    45 https://doi.org/10.1073/pnas.84.9.2901
    46 https://doi.org/10.1084/jem.175.2.437
    47 https://doi.org/10.1084/jem.175.5.1353
    48 https://doi.org/10.1084/jem.176.4.1215
    49 https://doi.org/10.1084/jem.177.1.165
    50 https://doi.org/10.1084/jem.178.2.381
    51 https://doi.org/10.1084/jem.178.6.1893
    52 https://doi.org/10.1093/intimm/5.3.317
    53 https://doi.org/10.1093/nar/12.3.1687
    54 https://doi.org/10.1111/j.1600-0625.1992.tb00004.x
    55 https://doi.org/10.1126/science.2113314
    56 https://doi.org/10.1126/science.7678351
    57 https://doi.org/10.1172/jci115840
    58 schema:datePublished 1994-07
    59 schema:datePublishedReg 1994-07-01
    60 schema:description Langerhans cells (LC) belong to the dendritic cell lineage and are the principal antigen-presenting cells of squamous epithelia. Short-term cultured LC (cLC) exhibit a marked augmented capacity to stimulate allogeneic T cells and acquire the ability to activate naive T cells, probably in relation to enhanced expression of accessory signals. In this study, we evaluated the expression of B7 costimulatory molecule (CD80) in human freshly isolated (fLC) and cLC at both the protein and mRNA level. Staining of frozen skin sections did not reveal any epidermal dendritic cell reactive with either of two different anti-B7 monoclonal antibodies. fLC in suspension did not exhibit any B7 staining as evaluated by two-color flow-cytometry analysis and immunoelectron microscopy. In contrast, LC that were cultured for 24-72 h displayed strong surface B7 reactivity with a characteristic patchy pattern. Treatment with dispase and trypsin did not reduce B7 staining of cLC. Following warming to 37 degrees C, cLC tagged with anti-B7 monoclonal antibody and gold-conjugated secondary antibody could internalize surface B7 by using the organelles of receptor-mediated endocytosis. B7 mRNA, detected by the reverse-transcriptase polymerase chain reaction technique, was expressed at a low level in purified (> 90% HLA-DR+) fLC but not in LC-depleted epidermal cells, and was markedly upregulated in purified cLC. The results indicate that 1) fLC do not express B7 protein on their surface, but acquire B7 during culture, 2) surface B7 is not sensitive to trypsin, 3) B7 expression is regulated primarily at the mRNA level, and 4) membrane B7 can be internalized within cLC. B7 molecule on CLC may be relevant to their increased antigen-presenting cell potency and ability to stimulate naive T lymphocytes.
    61 schema:genre research_article
    62 schema:inLanguage en
    63 schema:isAccessibleForFree true
    64 schema:isPartOf N4c96c2978db6431798f6874bf2065b42
    65 Neb9ea437ef7d4c93b5ea1e815307bd3c
    66 sg:journal.1020153
    67 schema:name Expression of B7 Costimulatory Molecule in Cultured Human Epidermal Langerhans Cells Is Regulated at the mRNA Level
    68 schema:pagination 54-59
    69 schema:productId N27c2877112444fdda52391c09adfea35
    70 N45058c15a1764d1e841f571eba187b52
    71 N481f1f7c1f5648cfb5eaf8ecb16553b1
    72 N658b0de49a4b41ccba9176c14f267b51
    73 Nd43e0984308c43aca0e4ecb346eb4431
    74 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048964060
    75 https://doi.org/10.1111/1523-1747.ep12389619
    76 schema:sdDatePublished 2019-04-10T19:45
    77 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    78 schema:sdPublisher N4c600192f5d94616858b37f18a1252b9
    79 schema:url http://www.nature.com/jid/journal/v103/n1/full/5611188a.html
    80 sgo:license sg:explorer/license/
    81 sgo:sdDataset articles
    82 rdf:type schema:ScholarlyArticle
    83 N049b526b67fd4e5298e480932effc940 rdf:first sg:person.01126305436.02
    84 rdf:rest N35e8aeb220844e0a9e63b86cc4e92870
    85 N05c178578673476d8bf9247ffe899a9d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    86 schema:name Antibodies, Monoclonal
    87 rdf:type schema:DefinedTerm
    88 N106def0c30544b86b502b6ce91f52c19 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    89 schema:name RNA, Messenger
    90 rdf:type schema:DefinedTerm
    91 N16346b24e5454539b17c58da54ed81e2 rdf:first sg:person.01252556266.57
    92 rdf:rest N8d560ed597ab4553a3d4de36faeca03a
    93 N27c2877112444fdda52391c09adfea35 schema:name readcube_id
    94 schema:value e941ea5ca57e26e202d7722c4713fa2bccd05e01d7a66372a80c43af78fa3930
    95 rdf:type schema:PropertyValue
    96 N30b9c3c5145c4b6c8900ba3d3a0bbc4f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Endocytosis
    98 rdf:type schema:DefinedTerm
    99 N31c347caf6c94444a4b7b556a3a55f9e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name In Situ Hybridization
    101 rdf:type schema:DefinedTerm
    102 N35e8aeb220844e0a9e63b86cc4e92870 rdf:first sg:person.016710673632.01
    103 rdf:rest N564f0a53408547079f6f66474c7532e6
    104 N367d4c3c77194927b73a918d8572f485 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Up-Regulation
    106 rdf:type schema:DefinedTerm
    107 N45058c15a1764d1e841f571eba187b52 schema:name pubmed_id
    108 schema:value 7517982
    109 rdf:type schema:PropertyValue
    110 N481f1f7c1f5648cfb5eaf8ecb16553b1 schema:name dimensions_id
    111 schema:value pub.1048964060
    112 rdf:type schema:PropertyValue
    113 N4c600192f5d94616858b37f18a1252b9 schema:name Springer Nature - SN SciGraph project
    114 rdf:type schema:Organization
    115 N4c96c2978db6431798f6874bf2065b42 schema:volumeNumber 103
    116 rdf:type schema:PublicationVolume
    117 N564f0a53408547079f6f66474c7532e6 rdf:first sg:person.01220110375.49
    118 rdf:rest Ne7a8b6fef42f416ea2f77db343d83214
    119 N648ac2a03583413f989651a95276bb6d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Cells, Cultured
    121 rdf:type schema:DefinedTerm
    122 N658b0de49a4b41ccba9176c14f267b51 schema:name nlm_unique_id
    123 schema:value 0426720
    124 rdf:type schema:PropertyValue
    125 N74c05d07407d49b2b38f3e73af490191 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Base Sequence
    127 rdf:type schema:DefinedTerm
    128 N853c081d546f4d0a866e1a82fda47512 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Microscopy, Immunoelectron
    130 rdf:type schema:DefinedTerm
    131 N8d560ed597ab4553a3d4de36faeca03a rdf:first sg:person.01315030632.54
    132 rdf:rest Nf1dc78bafb10471fb2e96330260484c3
    133 N94b094d702314875904db0228916b9e6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    134 schema:name Humans
    135 rdf:type schema:DefinedTerm
    136 Nb79650db94394777a0233959f2abc828 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Molecular Sequence Data
    138 rdf:type schema:DefinedTerm
    139 Nc4ba0bbf78ec4310b164ab27362bd972 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    140 schema:name Polymerase Chain Reaction
    141 rdf:type schema:DefinedTerm
    142 Nc74b32b5552c45b49de80386ea9cb424 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    143 schema:name B7-1 Antigen
    144 rdf:type schema:DefinedTerm
    145 Nc9a4fd10c9cb4237899980cf01d96e9c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    146 schema:name Langerhans Cells
    147 rdf:type schema:DefinedTerm
    148 Nd330c25949864e4c8048216e74a0e8cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name Flow Cytometry
    150 rdf:type schema:DefinedTerm
    151 Nd43e0984308c43aca0e4ecb346eb4431 schema:name doi
    152 schema:value 10.1111/1523-1747.ep12389619
    153 rdf:type schema:PropertyValue
    154 Ne7a8b6fef42f416ea2f77db343d83214 rdf:first sg:person.01167611267.01
    155 rdf:rest rdf:nil
    156 Neb9ea437ef7d4c93b5ea1e815307bd3c schema:issueNumber 1
    157 rdf:type schema:PublicationIssue
    158 Nec0e4cbe6dcd4413a03cc27481e08aee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    159 schema:name Fluorescent Antibody Technique
    160 rdf:type schema:DefinedTerm
    161 Nf1dc78bafb10471fb2e96330260484c3 rdf:first sg:person.01153321671.40
    162 rdf:rest N049b526b67fd4e5298e480932effc940
    163 Nf329a0eb975d4bc98e128fe550e1758f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    164 schema:name Gene Expression Regulation
    165 rdf:type schema:DefinedTerm
    166 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    167 schema:name Medical and Health Sciences
    168 rdf:type schema:DefinedTerm
    169 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
    170 schema:name Immunology
    171 rdf:type schema:DefinedTerm
    172 sg:journal.1020153 schema:issn 0022-202X
    173 1523-1747
    174 schema:name Journal of Investigative Dermatology
    175 rdf:type schema:Periodical
    176 sg:person.01126305436.02 schema:familyName Zacchi
    177 schema:givenName V
    178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01126305436.02
    179 rdf:type schema:Person
    180 sg:person.01153321671.40 schema:familyName Manfredini
    181 schema:givenName R
    182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153321671.40
    183 rdf:type schema:Person
    184 sg:person.01167611267.01 schema:familyName Giannetti
    185 schema:givenName A
    186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167611267.01
    187 rdf:type schema:Person
    188 sg:person.01220110375.49 schema:familyName Cossarizza
    189 schema:givenName A
    190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220110375.49
    191 rdf:type schema:Person
    192 sg:person.01252556266.57 schema:affiliation https://www.grid.ac/institutes/grid.7548.e
    193 schema:familyName Girolomoni
    194 schema:givenName G
    195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01252556266.57
    196 rdf:type schema:Person
    197 sg:person.01315030632.54 schema:familyName Zambruno
    198 schema:givenName G
    199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315030632.54
    200 rdf:type schema:Person
    201 sg:person.016710673632.01 schema:familyName Ferrari
    202 schema:givenName S
    203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016710673632.01
    204 rdf:type schema:Person
    205 sg:pub.10.1007/bf00200527 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027657509
    206 https://doi.org/10.1007/bf00200527
    207 rdf:type schema:CreativeWork
    208 sg:pub.10.1038/361082a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032702155
    209 https://doi.org/10.1038/361082a0
    210 rdf:type schema:CreativeWork
    211 sg:pub.10.1111/1523-1747.ep12319727 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005997357
    212 https://doi.org/10.1111/1523-1747.ep12319727
    213 rdf:type schema:CreativeWork
    214 sg:pub.10.1111/1523-1747.ep12462236 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007703237
    215 https://doi.org/10.1111/1523-1747.ep12462236
    216 rdf:type schema:CreativeWork
    217 sg:pub.10.1111/1523-1747.ep12462783 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020896416
    218 https://doi.org/10.1111/1523-1747.ep12462783
    219 rdf:type schema:CreativeWork
    220 sg:pub.10.1111/1523-1747.ep12469748 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022935908
    221 https://doi.org/10.1111/1523-1747.ep12469748
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1111/1523-1747.ep12469769 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030955715
    224 https://doi.org/10.1111/1523-1747.ep12469769
    225 rdf:type schema:CreativeWork
    226 sg:pub.10.1111/1523-1747.ep12616079 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016849724
    227 https://doi.org/10.1111/1523-1747.ep12616079
    228 rdf:type schema:CreativeWork
    229 sg:pub.10.1111/1523-1747.ep12616663 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012666866
    230 https://doi.org/10.1111/1523-1747.ep12616663
    231 rdf:type schema:CreativeWork
    232 sg:pub.10.1111/1523-1747.ep12874611 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006497117
    233 https://doi.org/10.1111/1523-1747.ep12874611
    234 rdf:type schema:CreativeWork
    235 https://app.dimensions.ai/details/publication/pub.1075302032 schema:CreativeWork
    236 https://app.dimensions.ai/details/publication/pub.1075960660 schema:CreativeWork
    237 https://app.dimensions.ai/details/publication/pub.1076028307 schema:CreativeWork
    238 https://app.dimensions.ai/details/publication/pub.1077604686 schema:CreativeWork
    239 https://app.dimensions.ai/details/publication/pub.1079368590 schema:CreativeWork
    240 https://app.dimensions.ai/details/publication/pub.1079522118 schema:CreativeWork
    241 https://app.dimensions.ai/details/publication/pub.1079834682 schema:CreativeWork
    242 https://app.dimensions.ai/details/publication/pub.1082462857 schema:CreativeWork
    243 https://app.dimensions.ai/details/publication/pub.1082463094 schema:CreativeWork
    244 https://app.dimensions.ai/details/publication/pub.1082463473 schema:CreativeWork
    245 https://app.dimensions.ai/details/publication/pub.1082798254 schema:CreativeWork
    246 https://app.dimensions.ai/details/publication/pub.1082813577 schema:CreativeWork
    247 https://app.dimensions.ai/details/publication/pub.1082826102 schema:CreativeWork
    248 https://doi.org/10.1002/eji.1830221207 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007991747
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1016/0952-7915(92)90075-p schema:sameAs https://app.dimensions.ai/details/publication/pub.1030628918
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1073/pnas.84.9.2901 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004375900
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1084/jem.175.2.437 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049243831
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1084/jem.175.5.1353 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050670867
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1084/jem.176.4.1215 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022004115
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1084/jem.177.1.165 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019400364
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1084/jem.178.2.381 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052243592
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1084/jem.178.6.1893 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011998739
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1093/intimm/5.3.317 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059711896
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1093/nar/12.3.1687 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012769682
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1111/j.1600-0625.1992.tb00004.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1027251796
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.1126/science.2113314 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062522797
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.1126/science.7678351 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062648365
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.1172/jci115840 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006005619
    277 rdf:type schema:CreativeWork
    278 https://www.grid.ac/institutes/grid.7548.e schema:alternateName University of Modena and Reggio Emilia
    279 schema:name Department of Dermatology, University of Modena, Italy.
    280 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...