Identification of Aberrantly Regulated Genes in Diseased Skin Using the cDNA Differential Display Technique View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1997-02

AUTHORS

M V Rivas, E D Jarvis, S Morisaki, H Carbonaro, A B Gottlieb, J G Krueger

ABSTRACT

It is hypothesized that psoriasis may be caused by aberrant gene expression. In an effort to identify and clone psoriasis-specific genes, we compared gene expression in normal, tape-stripped (wounded), and psoriatic skin using the cDNA differential display technique. Four genes not previously described in psoriasis--connexin 26, a gap junction protein; squamous cell carcinoma antigen-1 (SCCA1), a serine protease inhibitor; and mitochondrial NAD subunits 5 and 6--were identified as having very high expression levels in psoriatic skin. In situ hybridizations showed that connexin 26 mRNA was expressed 10-fold higher in psoriatic and 4-fold higher in tape-stripped epidermis than in controls. SCCA1 showed a 40-fold increase in mRNA expression, whereas mitochondrial NAD5 and NAD6 expression was increased 10- and 20-fold, respectively, in psoriatic skin. Northern blots confirmed the increased expression of connexin 26, SCCA1, and NAD6 genes in psoriatic skin. Immunohistochemistry showed that connexin 26 protein was strongly expressed in spinous keratinocytes from psoriatic skin and chronic wounds, but was absent in normal epidermis. These studies demonstrate the usefulness of this approach for identifying genes that are conditionally expressed in growth-activated human skin. More... »

PAGES

188-194

Identifiers

URI

http://scigraph.springernature.com/pub.10.1111/1523-1747.ep12334217

DOI

http://dx.doi.org/10.1111/1523-1747.ep12334217

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028270677

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9008232


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, Neoplasm", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Connexin 26", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Connexins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Complementary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Situ Hybridization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mitochondria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "NAD", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Psoriasis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Serpins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Skin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Wounds and Injuries", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Rockefeller University", 
          "id": "https://www.grid.ac/institutes/grid.134907.8", 
          "name": [
            "Laboratory for Investigative Dermatology, The Rockefeller University, New York, New York 10021-6399, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rivas", 
        "givenName": "M V", 
        "id": "sg:person.0700400040.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700400040.36"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Jarvis", 
        "givenName": "E D", 
        "id": "sg:person.011346222437.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011346222437.64"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Morisaki", 
        "givenName": "S", 
        "type": "Person"
      }, 
      {
        "familyName": "Carbonaro", 
        "givenName": "H", 
        "id": "sg:person.0742016401.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742016401.83"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Gottlieb", 
        "givenName": "A B", 
        "id": "sg:person.0614321162.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614321162.29"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Krueger", 
        "givenName": "J G", 
        "id": "sg:person.012632026177.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012632026177.48"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1385/0-89603-489-5:205", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004353414", 
          "https://doi.org/10.1385/0-89603-489-5:205"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.8.3147", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005581154"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0012-1606(91)90452-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006422442"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2133.1985.tb15258.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006842790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1001/archderm.1991.01680050115015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007914764"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/lm.2.2.62", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007982471"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/1523-1747.ep12471216", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008813460", 
          "https://doi.org/10.1111/1523-1747.ep12471216"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1097-0142(197710)40:4<1621::aid-cncr2820400435>3.0.co;2-i", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009545215"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.6.11.1491", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009900194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(87)90021-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013321498"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(05)80360-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013618994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0190-9622(94)70059-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013752398"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0560.1996.tb01430.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015894246"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/elps.1150110308", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016726318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/elps.1150110308", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016726318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0952-7915(95)80015-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017453053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.110.2.269", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018082861"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0190-9622(95)91286-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022737790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.77.9.5201", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024100683"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0896-6273(88)90146-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026970200"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/dbio.1994.1190", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027503206"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/314592a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029259530", 
          "https://doi.org/10.1038/314592a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.118.5.1213", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030840766"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/1523-1747.ep12505653", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033946416", 
          "https://doi.org/10.1111/1523-1747.ep12505653"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/1523-1747.ep12515095", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035255324", 
          "https://doi.org/10.1111/1523-1747.ep12515095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/1523-1747.ep12395202", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036770145", 
          "https://doi.org/10.1111/1523-1747.ep12395202"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/1523-1747.ep12393218", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038363245", 
          "https://doi.org/10.1111/1523-1747.ep12393218"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/290457a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038487698", 
          "https://doi.org/10.1038/290457a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/neu.480260112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040340377"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81282-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047804901"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0595-442", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049704206", 
          "https://doi.org/10.1038/nm0595-442"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-291x(05)81380-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051862625"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0738-081x(95)93817-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052127599"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1001/archderm.127.6.871", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054098468"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/abio.1987.9999", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054484911"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1354393", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062477287"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.3764430", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062621403"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0733-8635(18)30460-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078546999"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079785538", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1997-02", 
    "datePublishedReg": "1997-02-01", 
    "description": "It is hypothesized that psoriasis may be caused by aberrant gene expression. In an effort to identify and clone psoriasis-specific genes, we compared gene expression in normal, tape-stripped (wounded), and psoriatic skin using the cDNA differential display technique. Four genes not previously described in psoriasis--connexin 26, a gap junction protein; squamous cell carcinoma antigen-1 (SCCA1), a serine protease inhibitor; and mitochondrial NAD subunits 5 and 6--were identified as having very high expression levels in psoriatic skin. In situ hybridizations showed that connexin 26 mRNA was expressed 10-fold higher in psoriatic and 4-fold higher in tape-stripped epidermis than in controls. SCCA1 showed a 40-fold increase in mRNA expression, whereas mitochondrial NAD5 and NAD6 expression was increased 10- and 20-fold, respectively, in psoriatic skin. Northern blots confirmed the increased expression of connexin 26, SCCA1, and NAD6 genes in psoriatic skin. Immunohistochemistry showed that connexin 26 protein was strongly expressed in spinous keratinocytes from psoriatic skin and chronic wounds, but was absent in normal epidermis. These studies demonstrate the usefulness of this approach for identifying genes that are conditionally expressed in growth-activated human skin.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1111/1523-1747.ep12334217", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2512928", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2626152", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2423993", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1020153", 
        "issn": [
          "0022-202X", 
          "1523-1747"
        ], 
        "name": "Journal of Investigative Dermatology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "108"
      }
    ], 
    "name": "Identification of Aberrantly Regulated Genes in Diseased Skin Using the cDNA Differential Display Technique", 
    "pagination": "188-194", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "86acfbf6b47db4c84af17820ac5adf787ace4916da4f47f46d926d3c44fe8b83"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "9008232"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0426720"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1111/1523-1747.ep12334217"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1028270677"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1111/1523-1747.ep12334217", 
      "https://app.dimensions.ai/details/publication/pub.1028270677"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T23:12", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000424.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/jid/journal/v108/n2/full/5610279a.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12334217'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12334217'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12334217'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12334217'


 

This table displays all metadata directly associated to this object as RDF triples.

282 TRIPLES      21 PREDICATES      81 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1111/1523-1747.ep12334217 schema:about N1b81891c74664196adadee826a24cafd
2 N250fa9de24774b3c9a8a9b9fed140840
3 N4cee2ab95c714212a4fce23cf8706196
4 N54ac5c35ca92460cb63c88284ecbe89e
5 N67413c6c73e1401cb99596747ee7df88
6 N6bbb395fac0247c5a527d77cd6a57b08
7 N720317e819e941b780eb4e356a1e71b3
8 N7a526e8abe02488298c74e589912754a
9 Nb029e7ad01dd45a696ce706a30bec2eb
10 Nd5a2be6490234efb8115fd9b911e26d2
11 Nd6eda4476ff244ed93998c387f020bd8
12 Nead8969a33ee47c1a86fa0fb2c47070e
13 Nfb2a9dafa99b46028187a7dd3df92d9f
14 Nfde28ca89539440f96e3994d0302d286
15 anzsrc-for:06
16 anzsrc-for:0604
17 schema:author Nff17ff4286d44a9e8df65c566fe985f2
18 schema:citation sg:pub.10.1038/290457a0
19 sg:pub.10.1038/314592a0
20 sg:pub.10.1038/nm0595-442
21 sg:pub.10.1111/1523-1747.ep12393218
22 sg:pub.10.1111/1523-1747.ep12395202
23 sg:pub.10.1111/1523-1747.ep12471216
24 sg:pub.10.1111/1523-1747.ep12505653
25 sg:pub.10.1111/1523-1747.ep12515095
26 sg:pub.10.1385/0-89603-489-5:205
27 https://app.dimensions.ai/details/publication/pub.1079785538
28 https://doi.org/10.1001/archderm.127.6.871
29 https://doi.org/10.1001/archderm.1991.01680050115015
30 https://doi.org/10.1002/1097-0142(197710)40:4<1621::aid-cncr2820400435>3.0.co;2-i
31 https://doi.org/10.1002/elps.1150110308
32 https://doi.org/10.1002/neu.480260112
33 https://doi.org/10.1006/abio.1987.9999
34 https://doi.org/10.1006/dbio.1994.1190
35 https://doi.org/10.1016/0003-2697(87)90021-2
36 https://doi.org/10.1016/0012-1606(91)90452-9
37 https://doi.org/10.1016/0190-9622(95)91286-x
38 https://doi.org/10.1016/0738-081x(95)93817-8
39 https://doi.org/10.1016/0896-6273(88)90146-8
40 https://doi.org/10.1016/0952-7915(95)80015-8
41 https://doi.org/10.1016/s0006-291x(05)81380-4
42 https://doi.org/10.1016/s0022-2836(05)80360-2
43 https://doi.org/10.1016/s0092-8674(00)81282-9
44 https://doi.org/10.1016/s0190-9622(94)70059-1
45 https://doi.org/10.1016/s0733-8635(18)30460-1
46 https://doi.org/10.1073/pnas.77.9.5201
47 https://doi.org/10.1073/pnas.92.8.3147
48 https://doi.org/10.1083/jcb.110.2.269
49 https://doi.org/10.1083/jcb.118.5.1213
50 https://doi.org/10.1091/mbc.6.11.1491
51 https://doi.org/10.1101/lm.2.2.62
52 https://doi.org/10.1111/j.1365-2133.1985.tb15258.x
53 https://doi.org/10.1111/j.1600-0560.1996.tb01430.x
54 https://doi.org/10.1126/science.1354393
55 https://doi.org/10.1126/science.3764430
56 schema:datePublished 1997-02
57 schema:datePublishedReg 1997-02-01
58 schema:description It is hypothesized that psoriasis may be caused by aberrant gene expression. In an effort to identify and clone psoriasis-specific genes, we compared gene expression in normal, tape-stripped (wounded), and psoriatic skin using the cDNA differential display technique. Four genes not previously described in psoriasis--connexin 26, a gap junction protein; squamous cell carcinoma antigen-1 (SCCA1), a serine protease inhibitor; and mitochondrial NAD subunits 5 and 6--were identified as having very high expression levels in psoriatic skin. In situ hybridizations showed that connexin 26 mRNA was expressed 10-fold higher in psoriatic and 4-fold higher in tape-stripped epidermis than in controls. SCCA1 showed a 40-fold increase in mRNA expression, whereas mitochondrial NAD5 and NAD6 expression was increased 10- and 20-fold, respectively, in psoriatic skin. Northern blots confirmed the increased expression of connexin 26, SCCA1, and NAD6 genes in psoriatic skin. Immunohistochemistry showed that connexin 26 protein was strongly expressed in spinous keratinocytes from psoriatic skin and chronic wounds, but was absent in normal epidermis. These studies demonstrate the usefulness of this approach for identifying genes that are conditionally expressed in growth-activated human skin.
59 schema:genre research_article
60 schema:inLanguage en
61 schema:isAccessibleForFree true
62 schema:isPartOf N1e4e4b78071d4aa5a19fc997e19f4891
63 N9d217bf7df1447f986c91ec332007fa6
64 sg:journal.1020153
65 schema:name Identification of Aberrantly Regulated Genes in Diseased Skin Using the cDNA Differential Display Technique
66 schema:pagination 188-194
67 schema:productId N226fd9d6ecd648f4abf8dd357eac4d6c
68 N4474291b3b4448b790b24cfce3b76ee9
69 N9339475f8baf459cb702c743a1163f08
70 Na68c5b9fd80d48089dd6466b4aed0948
71 Ne15c129936e646279402ee29e81a2a03
72 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028270677
73 https://doi.org/10.1111/1523-1747.ep12334217
74 schema:sdDatePublished 2019-04-10T23:12
75 schema:sdLicense https://scigraph.springernature.com/explorer/license/
76 schema:sdPublisher Nee5a64f9b5d84a209de9dba4de6e8d90
77 schema:url http://www.nature.com/jid/journal/v108/n2/full/5610279a.html
78 sgo:license sg:explorer/license/
79 sgo:sdDataset articles
80 rdf:type schema:ScholarlyArticle
81 N15f46a3072624981adfa95dec4f30a52 schema:familyName Morisaki
82 schema:givenName S
83 rdf:type schema:Person
84 N1b81891c74664196adadee826a24cafd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Mitochondria
86 rdf:type schema:DefinedTerm
87 N1e4e4b78071d4aa5a19fc997e19f4891 schema:volumeNumber 108
88 rdf:type schema:PublicationVolume
89 N226fd9d6ecd648f4abf8dd357eac4d6c schema:name pubmed_id
90 schema:value 9008232
91 rdf:type schema:PropertyValue
92 N250fa9de24774b3c9a8a9b9fed140840 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name In Situ Hybridization
94 rdf:type schema:DefinedTerm
95 N29472cf146be45aeab09b990b54f0dd2 rdf:first sg:person.0742016401.83
96 rdf:rest N8bca2e9ab45a4c6a89b4432bbf60a658
97 N4474291b3b4448b790b24cfce3b76ee9 schema:name readcube_id
98 schema:value 86acfbf6b47db4c84af17820ac5adf787ace4916da4f47f46d926d3c44fe8b83
99 rdf:type schema:PropertyValue
100 N4cee2ab95c714212a4fce23cf8706196 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Gene Expression Regulation
102 rdf:type schema:DefinedTerm
103 N54ac5c35ca92460cb63c88284ecbe89e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Connexin 26
105 rdf:type schema:DefinedTerm
106 N67413c6c73e1401cb99596747ee7df88 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Antigens, Neoplasm
108 rdf:type schema:DefinedTerm
109 N6bbb395fac0247c5a527d77cd6a57b08 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Connexins
111 rdf:type schema:DefinedTerm
112 N720317e819e941b780eb4e356a1e71b3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Serpins
114 rdf:type schema:DefinedTerm
115 N7a526e8abe02488298c74e589912754a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Psoriasis
117 rdf:type schema:DefinedTerm
118 N8bca2e9ab45a4c6a89b4432bbf60a658 rdf:first sg:person.0614321162.29
119 rdf:rest Nc4411c49947748d8b050a54c60292890
120 N9339475f8baf459cb702c743a1163f08 schema:name doi
121 schema:value 10.1111/1523-1747.ep12334217
122 rdf:type schema:PropertyValue
123 N9d217bf7df1447f986c91ec332007fa6 schema:issueNumber 2
124 rdf:type schema:PublicationIssue
125 Na68c5b9fd80d48089dd6466b4aed0948 schema:name nlm_unique_id
126 schema:value 0426720
127 rdf:type schema:PropertyValue
128 Nb029e7ad01dd45a696ce706a30bec2eb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Sequence Analysis
130 rdf:type schema:DefinedTerm
131 Nc1939a31c8fe475d99a97d38af4739f4 rdf:first N15f46a3072624981adfa95dec4f30a52
132 rdf:rest N29472cf146be45aeab09b990b54f0dd2
133 Nc4411c49947748d8b050a54c60292890 rdf:first sg:person.012632026177.48
134 rdf:rest rdf:nil
135 Nd3d27425df5c4ca9a680324a201eff0e rdf:first sg:person.011346222437.64
136 rdf:rest Nc1939a31c8fe475d99a97d38af4739f4
137 Nd5a2be6490234efb8115fd9b911e26d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Humans
139 rdf:type schema:DefinedTerm
140 Nd6eda4476ff244ed93998c387f020bd8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Skin
142 rdf:type schema:DefinedTerm
143 Ne15c129936e646279402ee29e81a2a03 schema:name dimensions_id
144 schema:value pub.1028270677
145 rdf:type schema:PropertyValue
146 Nead8969a33ee47c1a86fa0fb2c47070e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name DNA, Complementary
148 rdf:type schema:DefinedTerm
149 Nee5a64f9b5d84a209de9dba4de6e8d90 schema:name Springer Nature - SN SciGraph project
150 rdf:type schema:Organization
151 Nfb2a9dafa99b46028187a7dd3df92d9f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Wounds and Injuries
153 rdf:type schema:DefinedTerm
154 Nfde28ca89539440f96e3994d0302d286 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name NAD
156 rdf:type schema:DefinedTerm
157 Nff17ff4286d44a9e8df65c566fe985f2 rdf:first sg:person.0700400040.36
158 rdf:rest Nd3d27425df5c4ca9a680324a201eff0e
159 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
160 schema:name Biological Sciences
161 rdf:type schema:DefinedTerm
162 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
163 schema:name Genetics
164 rdf:type schema:DefinedTerm
165 sg:grant.2423993 http://pending.schema.org/fundedItem sg:pub.10.1111/1523-1747.ep12334217
166 rdf:type schema:MonetaryGrant
167 sg:grant.2512928 http://pending.schema.org/fundedItem sg:pub.10.1111/1523-1747.ep12334217
168 rdf:type schema:MonetaryGrant
169 sg:grant.2626152 http://pending.schema.org/fundedItem sg:pub.10.1111/1523-1747.ep12334217
170 rdf:type schema:MonetaryGrant
171 sg:journal.1020153 schema:issn 0022-202X
172 1523-1747
173 schema:name Journal of Investigative Dermatology
174 rdf:type schema:Periodical
175 sg:person.011346222437.64 schema:familyName Jarvis
176 schema:givenName E D
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011346222437.64
178 rdf:type schema:Person
179 sg:person.012632026177.48 schema:familyName Krueger
180 schema:givenName J G
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012632026177.48
182 rdf:type schema:Person
183 sg:person.0614321162.29 schema:familyName Gottlieb
184 schema:givenName A B
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614321162.29
186 rdf:type schema:Person
187 sg:person.0700400040.36 schema:affiliation https://www.grid.ac/institutes/grid.134907.8
188 schema:familyName Rivas
189 schema:givenName M V
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700400040.36
191 rdf:type schema:Person
192 sg:person.0742016401.83 schema:familyName Carbonaro
193 schema:givenName H
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742016401.83
195 rdf:type schema:Person
196 sg:pub.10.1038/290457a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038487698
197 https://doi.org/10.1038/290457a0
198 rdf:type schema:CreativeWork
199 sg:pub.10.1038/314592a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029259530
200 https://doi.org/10.1038/314592a0
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/nm0595-442 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049704206
203 https://doi.org/10.1038/nm0595-442
204 rdf:type schema:CreativeWork
205 sg:pub.10.1111/1523-1747.ep12393218 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038363245
206 https://doi.org/10.1111/1523-1747.ep12393218
207 rdf:type schema:CreativeWork
208 sg:pub.10.1111/1523-1747.ep12395202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036770145
209 https://doi.org/10.1111/1523-1747.ep12395202
210 rdf:type schema:CreativeWork
211 sg:pub.10.1111/1523-1747.ep12471216 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008813460
212 https://doi.org/10.1111/1523-1747.ep12471216
213 rdf:type schema:CreativeWork
214 sg:pub.10.1111/1523-1747.ep12505653 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033946416
215 https://doi.org/10.1111/1523-1747.ep12505653
216 rdf:type schema:CreativeWork
217 sg:pub.10.1111/1523-1747.ep12515095 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035255324
218 https://doi.org/10.1111/1523-1747.ep12515095
219 rdf:type schema:CreativeWork
220 sg:pub.10.1385/0-89603-489-5:205 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004353414
221 https://doi.org/10.1385/0-89603-489-5:205
222 rdf:type schema:CreativeWork
223 https://app.dimensions.ai/details/publication/pub.1079785538 schema:CreativeWork
224 https://doi.org/10.1001/archderm.127.6.871 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054098468
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1001/archderm.1991.01680050115015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007914764
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1002/1097-0142(197710)40:4<1621::aid-cncr2820400435>3.0.co;2-i schema:sameAs https://app.dimensions.ai/details/publication/pub.1009545215
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1002/elps.1150110308 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016726318
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1002/neu.480260112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040340377
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1006/abio.1987.9999 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054484911
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1006/dbio.1994.1190 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027503206
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/0003-2697(87)90021-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013321498
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/0012-1606(91)90452-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006422442
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/0190-9622(95)91286-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022737790
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/0738-081x(95)93817-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052127599
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/0896-6273(88)90146-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026970200
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/0952-7915(95)80015-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017453053
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/s0006-291x(05)81380-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051862625
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/s0022-2836(05)80360-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013618994
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/s0092-8674(00)81282-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047804901
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1016/s0190-9622(94)70059-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013752398
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1016/s0733-8635(18)30460-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078546999
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1073/pnas.77.9.5201 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024100683
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1073/pnas.92.8.3147 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005581154
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1083/jcb.110.2.269 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018082861
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1083/jcb.118.5.1213 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030840766
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1091/mbc.6.11.1491 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009900194
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1101/lm.2.2.62 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007982471
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1111/j.1365-2133.1985.tb15258.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006842790
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1111/j.1600-0560.1996.tb01430.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1015894246
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1126/science.1354393 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062477287
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1126/science.3764430 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062621403
279 rdf:type schema:CreativeWork
280 https://www.grid.ac/institutes/grid.134907.8 schema:alternateName Rockefeller University
281 schema:name Laboratory for Investigative Dermatology, The Rockefeller University, New York, New York 10021-6399, USA.
282 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...