Successful Treatment of Chronic Leg Ulcers with Epidermal Equivalents Generated from Cultured Autologous Outer Root Sheath Cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1996-07

AUTHORS

A Limat, D Mauri, T Hunziker

ABSTRACT

The outer root sheath cells of hair follicles can substitute for interfollicular epidermal keratinocytes, as during healing of skin wounds when these cells migrate onto the denuded area and contribute to epidermal regeneration. Using improved culture techniques, we generated epidermal equivalents from cultured outer root sheath cells of patients suffering from recalcitrant chronic leg ulcers, primarily of vascular origin. In such epidermal equivalents, tissue organization as well as immunolocalization of epidermal differentiation products (keratin 10, involucrin, filaggrin) and integrins were indistinguishable from normal epidermis. As determined by the number of bromodeoxyuridine-incorporating cells, the basal layer contained a large compartment of proliferative cells irrespective of donor age. FACS analysis of the outer root sheath cells, used to prepare the epidermal equivalents, disclosed a fraction of small cells with enhanced expression of beta1-integrin, a potential stem cell marker. in contrast to acute wounds, a major definitive take of grafted cultured autologous keratinocytes has not been convincingly demonstrated in chronic wounds. In a pilot study, grafting of epidermal equivalents generated in vitro from autologous outer root sheath cells on 11 ulcers in five patients resulted in a definitive take rate of about 80%, with subsequent complete healing within 2 to 3 wk of five out of seven ulcers grafted with densely arranged cultures. This improvement in the treatment of chronic leg ulcers with cultured autologous keratinocytes probably depends on the large compartment of proliferative cells as well as on a well-developed horny layer which prevents disintegration of the grafts. Practical advantages of the new technique are its noninvasiveness, the lack of need for surgical facilities or anesthesia, and a short immobilization period after grafting. More... »

PAGES

128-135

Identifiers

URI

http://scigraph.springernature.com/pub.10.1111/1523-1747.ep12298415

DOI

http://dx.doi.org/10.1111/1523-1747.ep12298415

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044507255

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8752851


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aged", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aged, 80 and over", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Transplantation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chronic Disease", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epidermis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hair Follicle", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leg Ulcer", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reference Values", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transplantation, Autologous", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Treatment Outcome", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Bern", 
          "id": "https://www.grid.ac/institutes/grid.5734.5", 
          "name": [
            "Dermatologic Clinic, University of Bern, Switzerland."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Limat", 
        "givenName": "A", 
        "id": "sg:person.01342517334.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342517334.20"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Mauri", 
        "givenName": "D", 
        "type": "Person"
      }, 
      {
        "familyName": "Hunziker", 
        "givenName": "T", 
        "id": "sg:person.01313076406.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313076406.53"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1097/00006534-198907000-00001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006092701"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00006534-198907000-00001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006092701"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1524-4725.1988.tb03343.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006555064"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1524-4725.1988.tb03343.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006555064"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2230.1986.tb00526.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007509526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02505040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007886478", 
          "https://doi.org/10.1007/bf02505040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02505040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007886478", 
          "https://doi.org/10.1007/bf02505040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2133.1987.tb07491.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008050690"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.156.6.1755", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008388622"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2133.1990.tb01844.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009509343"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(94)90383-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012133473"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(82)90400-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012378105"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0625.1993.tb00033.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012717291"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/1523-1747.ep12371671", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020629358", 
          "https://doi.org/10.1111/1523-1747.ep12371671"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0165-2478(91)90220-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021762448"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0165-2478(91)90220-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021762448"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2230.1993.tb02240.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022406046"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-0436.1986.tb00395.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022830756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0190-9622(91)70015-t", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024234633"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0190-9622(91)70188-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024578573"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/b978-0-12-505263-4.50012-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025367999"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00372010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025755929", 
          "https://doi.org/10.1007/bf00372010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0190-9622(89)70160-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025994533"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0190-9622(90)70197-p", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026208543"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/jid.1955.112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027003559", 
          "https://doi.org/10.1038/jid.1955.112"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1524-4725.1994.tb03715.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027276932"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1524-4725.1994.tb03715.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027276932"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(81)90006-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027678149"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(81)90006-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027678149"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2133.1981.tb01704.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028947010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00372133", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031341458", 
          "https://doi.org/10.1007/bf00372133"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/1523-1747.ep12455548", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031594384", 
          "https://doi.org/10.1111/1523-1747.ep12455548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(93)90251-k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031600813"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/1523-1747.ep12616134", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033239574", 
          "https://doi.org/10.1111/1523-1747.ep12616134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0190-9622(87)70263-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033436731"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.122.2.417", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033701108"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-4827(91)90357-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034538314"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(90)90696-c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041327415"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007890-199504150-00020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044512380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00007890-199504150-00020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044512380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0012-1606(88)90356-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045613282"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-0436.1987.tb00174.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046314841"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0190-9622(87)70199-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046693939"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0002-9610(99)80258-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046728298"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1111/1523-1747.ep12312406", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048170443", 
          "https://doi.org/10.1111/1523-1747.ep12312406"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00000637-199102000-00015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050477884"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00000637-199102000-00015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050477884"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(82)90324-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050794369"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1749-6632.1991.tb24385.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051385654"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0190-9622(86)70048-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052294892"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s001050050297", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054510951", 
          "https://doi.org/10.1007/s001050050297"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s001050050297", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054510951", 
          "https://doi.org/10.1007/s001050050297"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078508531", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078849412", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078890913", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078891035", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079785942", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079807696", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079819534", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082696819", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1996-07", 
    "datePublishedReg": "1996-07-01", 
    "description": "The outer root sheath cells of hair follicles can substitute for interfollicular epidermal keratinocytes, as during healing of skin wounds when these cells migrate onto the denuded area and contribute to epidermal regeneration. Using improved culture techniques, we generated epidermal equivalents from cultured outer root sheath cells of patients suffering from recalcitrant chronic leg ulcers, primarily of vascular origin. In such epidermal equivalents, tissue organization as well as immunolocalization of epidermal differentiation products (keratin 10, involucrin, filaggrin) and integrins were indistinguishable from normal epidermis. As determined by the number of bromodeoxyuridine-incorporating cells, the basal layer contained a large compartment of proliferative cells irrespective of donor age. FACS analysis of the outer root sheath cells, used to prepare the epidermal equivalents, disclosed a fraction of small cells with enhanced expression of beta1-integrin, a potential stem cell marker. in contrast to acute wounds, a major definitive take of grafted cultured autologous keratinocytes has not been convincingly demonstrated in chronic wounds. In a pilot study, grafting of epidermal equivalents generated in vitro from autologous outer root sheath cells on 11 ulcers in five patients resulted in a definitive take rate of about 80%, with subsequent complete healing within 2 to 3 wk of five out of seven ulcers grafted with densely arranged cultures. This improvement in the treatment of chronic leg ulcers with cultured autologous keratinocytes probably depends on the large compartment of proliferative cells as well as on a well-developed horny layer which prevents disintegration of the grafts. Practical advantages of the new technique are its noninvasiveness, the lack of need for surgical facilities or anesthesia, and a short immobilization period after grafting.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1111/1523-1747.ep12298415", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1020153", 
        "issn": [
          "0022-202X", 
          "1523-1747"
        ], 
        "name": "Journal of Investigative Dermatology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "107"
      }
    ], 
    "name": "Successful Treatment of Chronic Leg Ulcers with Epidermal Equivalents Generated from Cultured Autologous Outer Root Sheath Cells", 
    "pagination": "128-135", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d40123eb547bcafda39628394cf643beb0a00374e1246a27e2e2c8a982b1584d"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "8752851"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0426720"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1111/1523-1747.ep12298415"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1044507255"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1111/1523-1747.ep12298415", 
      "https://app.dimensions.ai/details/publication/pub.1044507255"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T18:08", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000426.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/jid/journal/v107/n1/full/5618236a.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12298415'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12298415'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12298415'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1111/1523-1747.ep12298415'


 

This table displays all metadata directly associated to this object as RDF triples.

286 TRIPLES      21 PREDICATES      93 URIs      34 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1111/1523-1747.ep12298415 schema:about N1123200589ae4daba1d88d7317d86b90
2 N177afb98c9fe4a71bc4a8c5c735667b9
3 N3eb4dabef16c41d894b1268b6ee4c656
4 N493c350379a3402ea1cc39ae0fa6ba3f
5 N56d75902f2944460b9b6df5416ff032d
6 N5a2a4b0f5556492aa2cf1a2fa13b18d9
7 N7150bc226073458b9efbf42a94979fb5
8 Nac3b232e22824c2bae53ad159d9170d1
9 Nb4070690b8624624b7a08b964325bc2c
10 Nd4963c26941e4301a4cc525377d58a36
11 Ne74f128edf0d4873afe26007efb26296
12 Nf73188f7aef943b8882ee2685560304e
13 Nfbebac6e81ca4c0a9ecaa92d4b5ac37c
14 anzsrc-for:11
15 anzsrc-for:1103
16 schema:author Nf125209cfbbe452893f4b8ae72fafb15
17 schema:citation sg:pub.10.1007/bf00372010
18 sg:pub.10.1007/bf00372133
19 sg:pub.10.1007/bf02505040
20 sg:pub.10.1007/s001050050297
21 sg:pub.10.1038/jid.1955.112
22 sg:pub.10.1111/1523-1747.ep12312406
23 sg:pub.10.1111/1523-1747.ep12371671
24 sg:pub.10.1111/1523-1747.ep12455548
25 sg:pub.10.1111/1523-1747.ep12616134
26 https://app.dimensions.ai/details/publication/pub.1078508531
27 https://app.dimensions.ai/details/publication/pub.1078849412
28 https://app.dimensions.ai/details/publication/pub.1078890913
29 https://app.dimensions.ai/details/publication/pub.1078891035
30 https://app.dimensions.ai/details/publication/pub.1079785942
31 https://app.dimensions.ai/details/publication/pub.1079807696
32 https://app.dimensions.ai/details/publication/pub.1079819534
33 https://app.dimensions.ai/details/publication/pub.1082696819
34 https://doi.org/10.1016/0012-1606(88)90356-9
35 https://doi.org/10.1016/0014-4827(91)90357-z
36 https://doi.org/10.1016/0092-8674(82)90324-5
37 https://doi.org/10.1016/0092-8674(82)90400-7
38 https://doi.org/10.1016/0092-8674(90)90696-c
39 https://doi.org/10.1016/0092-8674(93)90251-k
40 https://doi.org/10.1016/0092-8674(94)90383-2
41 https://doi.org/10.1016/0165-2478(91)90220-5
42 https://doi.org/10.1016/0190-9622(90)70197-p
43 https://doi.org/10.1016/0190-9622(91)70015-t
44 https://doi.org/10.1016/0190-9622(91)70188-8
45 https://doi.org/10.1016/b978-0-12-505263-4.50012-1
46 https://doi.org/10.1016/s0002-9610(99)80258-x
47 https://doi.org/10.1016/s0140-6736(81)90006-4
48 https://doi.org/10.1016/s0190-9622(86)70048-0
49 https://doi.org/10.1016/s0190-9622(87)70199-6
50 https://doi.org/10.1016/s0190-9622(87)70263-1
51 https://doi.org/10.1016/s0190-9622(89)70160-2
52 https://doi.org/10.1083/jcb.122.2.417
53 https://doi.org/10.1084/jem.156.6.1755
54 https://doi.org/10.1097/00000637-199102000-00015
55 https://doi.org/10.1097/00006534-198907000-00001
56 https://doi.org/10.1097/00007890-199504150-00020
57 https://doi.org/10.1111/j.1365-2133.1981.tb01704.x
58 https://doi.org/10.1111/j.1365-2133.1987.tb07491.x
59 https://doi.org/10.1111/j.1365-2133.1990.tb01844.x
60 https://doi.org/10.1111/j.1365-2230.1986.tb00526.x
61 https://doi.org/10.1111/j.1365-2230.1993.tb02240.x
62 https://doi.org/10.1111/j.1432-0436.1986.tb00395.x
63 https://doi.org/10.1111/j.1432-0436.1987.tb00174.x
64 https://doi.org/10.1111/j.1524-4725.1988.tb03343.x
65 https://doi.org/10.1111/j.1524-4725.1994.tb03715.x
66 https://doi.org/10.1111/j.1600-0625.1993.tb00033.x
67 https://doi.org/10.1111/j.1749-6632.1991.tb24385.x
68 schema:datePublished 1996-07
69 schema:datePublishedReg 1996-07-01
70 schema:description The outer root sheath cells of hair follicles can substitute for interfollicular epidermal keratinocytes, as during healing of skin wounds when these cells migrate onto the denuded area and contribute to epidermal regeneration. Using improved culture techniques, we generated epidermal equivalents from cultured outer root sheath cells of patients suffering from recalcitrant chronic leg ulcers, primarily of vascular origin. In such epidermal equivalents, tissue organization as well as immunolocalization of epidermal differentiation products (keratin 10, involucrin, filaggrin) and integrins were indistinguishable from normal epidermis. As determined by the number of bromodeoxyuridine-incorporating cells, the basal layer contained a large compartment of proliferative cells irrespective of donor age. FACS analysis of the outer root sheath cells, used to prepare the epidermal equivalents, disclosed a fraction of small cells with enhanced expression of beta1-integrin, a potential stem cell marker. in contrast to acute wounds, a major definitive take of grafted cultured autologous keratinocytes has not been convincingly demonstrated in chronic wounds. In a pilot study, grafting of epidermal equivalents generated in vitro from autologous outer root sheath cells on 11 ulcers in five patients resulted in a definitive take rate of about 80%, with subsequent complete healing within 2 to 3 wk of five out of seven ulcers grafted with densely arranged cultures. This improvement in the treatment of chronic leg ulcers with cultured autologous keratinocytes probably depends on the large compartment of proliferative cells as well as on a well-developed horny layer which prevents disintegration of the grafts. Practical advantages of the new technique are its noninvasiveness, the lack of need for surgical facilities or anesthesia, and a short immobilization period after grafting.
71 schema:genre research_article
72 schema:inLanguage en
73 schema:isAccessibleForFree true
74 schema:isPartOf Na56afc03d0b9489499dcb7cab10fc4a5
75 Nfe1bbaa022fe47bc81c361f0e58aca7d
76 sg:journal.1020153
77 schema:name Successful Treatment of Chronic Leg Ulcers with Epidermal Equivalents Generated from Cultured Autologous Outer Root Sheath Cells
78 schema:pagination 128-135
79 schema:productId N0758c57c8d9e415696d2e6c72e9cf8b1
80 N18c7697bfbd1436ab8e5127004ec0552
81 N479ee50ee65e4df58971bed525a09436
82 N7ec3c1f61a224d59a37b6c8e63b7999f
83 N9b652965335947738fc52206377c7a39
84 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044507255
85 https://doi.org/10.1111/1523-1747.ep12298415
86 schema:sdDatePublished 2019-04-10T18:08
87 schema:sdLicense https://scigraph.springernature.com/explorer/license/
88 schema:sdPublisher N2cfe8daaa7d0452baac8e5e37b228829
89 schema:url http://www.nature.com/jid/journal/v107/n1/full/5618236a.html
90 sgo:license sg:explorer/license/
91 sgo:sdDataset articles
92 rdf:type schema:ScholarlyArticle
93 N0758c57c8d9e415696d2e6c72e9cf8b1 schema:name doi
94 schema:value 10.1111/1523-1747.ep12298415
95 rdf:type schema:PropertyValue
96 N1123200589ae4daba1d88d7317d86b90 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Aged
98 rdf:type schema:DefinedTerm
99 N177afb98c9fe4a71bc4a8c5c735667b9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Treatment Outcome
101 rdf:type schema:DefinedTerm
102 N18c7697bfbd1436ab8e5127004ec0552 schema:name pubmed_id
103 schema:value 8752851
104 rdf:type schema:PropertyValue
105 N2cfe8daaa7d0452baac8e5e37b228829 schema:name Springer Nature - SN SciGraph project
106 rdf:type schema:Organization
107 N3607519aa50542e2be09e6a662f454f3 rdf:first sg:person.01313076406.53
108 rdf:rest rdf:nil
109 N3e6059bbac544d3f8a8d0b71fda5e5a9 schema:familyName Mauri
110 schema:givenName D
111 rdf:type schema:Person
112 N3eb4dabef16c41d894b1268b6ee4c656 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Cell Transplantation
114 rdf:type schema:DefinedTerm
115 N479ee50ee65e4df58971bed525a09436 schema:name nlm_unique_id
116 schema:value 0426720
117 rdf:type schema:PropertyValue
118 N493c350379a3402ea1cc39ae0fa6ba3f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Reference Values
120 rdf:type schema:DefinedTerm
121 N548ab1b643444a25b354d73d50c341b2 rdf:first N3e6059bbac544d3f8a8d0b71fda5e5a9
122 rdf:rest N3607519aa50542e2be09e6a662f454f3
123 N56d75902f2944460b9b6df5416ff032d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Cell Differentiation
125 rdf:type schema:DefinedTerm
126 N5a2a4b0f5556492aa2cf1a2fa13b18d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Chronic Disease
128 rdf:type schema:DefinedTerm
129 N7150bc226073458b9efbf42a94979fb5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Leg Ulcer
131 rdf:type schema:DefinedTerm
132 N7ec3c1f61a224d59a37b6c8e63b7999f schema:name dimensions_id
133 schema:value pub.1044507255
134 rdf:type schema:PropertyValue
135 N9b652965335947738fc52206377c7a39 schema:name readcube_id
136 schema:value d40123eb547bcafda39628394cf643beb0a00374e1246a27e2e2c8a982b1584d
137 rdf:type schema:PropertyValue
138 Na56afc03d0b9489499dcb7cab10fc4a5 schema:issueNumber 1
139 rdf:type schema:PublicationIssue
140 Nac3b232e22824c2bae53ad159d9170d1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Aged, 80 and over
142 rdf:type schema:DefinedTerm
143 Nb4070690b8624624b7a08b964325bc2c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Transplantation, Autologous
145 rdf:type schema:DefinedTerm
146 Nd4963c26941e4301a4cc525377d58a36 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Cells, Cultured
148 rdf:type schema:DefinedTerm
149 Ne74f128edf0d4873afe26007efb26296 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Humans
151 rdf:type schema:DefinedTerm
152 Nf125209cfbbe452893f4b8ae72fafb15 rdf:first sg:person.01342517334.20
153 rdf:rest N548ab1b643444a25b354d73d50c341b2
154 Nf73188f7aef943b8882ee2685560304e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Epidermis
156 rdf:type schema:DefinedTerm
157 Nfbebac6e81ca4c0a9ecaa92d4b5ac37c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Hair Follicle
159 rdf:type schema:DefinedTerm
160 Nfe1bbaa022fe47bc81c361f0e58aca7d schema:volumeNumber 107
161 rdf:type schema:PublicationVolume
162 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
163 schema:name Medical and Health Sciences
164 rdf:type schema:DefinedTerm
165 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
166 schema:name Clinical Sciences
167 rdf:type schema:DefinedTerm
168 sg:journal.1020153 schema:issn 0022-202X
169 1523-1747
170 schema:name Journal of Investigative Dermatology
171 rdf:type schema:Periodical
172 sg:person.01313076406.53 schema:familyName Hunziker
173 schema:givenName T
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313076406.53
175 rdf:type schema:Person
176 sg:person.01342517334.20 schema:affiliation https://www.grid.ac/institutes/grid.5734.5
177 schema:familyName Limat
178 schema:givenName A
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342517334.20
180 rdf:type schema:Person
181 sg:pub.10.1007/bf00372010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025755929
182 https://doi.org/10.1007/bf00372010
183 rdf:type schema:CreativeWork
184 sg:pub.10.1007/bf00372133 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031341458
185 https://doi.org/10.1007/bf00372133
186 rdf:type schema:CreativeWork
187 sg:pub.10.1007/bf02505040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007886478
188 https://doi.org/10.1007/bf02505040
189 rdf:type schema:CreativeWork
190 sg:pub.10.1007/s001050050297 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054510951
191 https://doi.org/10.1007/s001050050297
192 rdf:type schema:CreativeWork
193 sg:pub.10.1038/jid.1955.112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027003559
194 https://doi.org/10.1038/jid.1955.112
195 rdf:type schema:CreativeWork
196 sg:pub.10.1111/1523-1747.ep12312406 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048170443
197 https://doi.org/10.1111/1523-1747.ep12312406
198 rdf:type schema:CreativeWork
199 sg:pub.10.1111/1523-1747.ep12371671 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020629358
200 https://doi.org/10.1111/1523-1747.ep12371671
201 rdf:type schema:CreativeWork
202 sg:pub.10.1111/1523-1747.ep12455548 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031594384
203 https://doi.org/10.1111/1523-1747.ep12455548
204 rdf:type schema:CreativeWork
205 sg:pub.10.1111/1523-1747.ep12616134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033239574
206 https://doi.org/10.1111/1523-1747.ep12616134
207 rdf:type schema:CreativeWork
208 https://app.dimensions.ai/details/publication/pub.1078508531 schema:CreativeWork
209 https://app.dimensions.ai/details/publication/pub.1078849412 schema:CreativeWork
210 https://app.dimensions.ai/details/publication/pub.1078890913 schema:CreativeWork
211 https://app.dimensions.ai/details/publication/pub.1078891035 schema:CreativeWork
212 https://app.dimensions.ai/details/publication/pub.1079785942 schema:CreativeWork
213 https://app.dimensions.ai/details/publication/pub.1079807696 schema:CreativeWork
214 https://app.dimensions.ai/details/publication/pub.1079819534 schema:CreativeWork
215 https://app.dimensions.ai/details/publication/pub.1082696819 schema:CreativeWork
216 https://doi.org/10.1016/0012-1606(88)90356-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045613282
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/0014-4827(91)90357-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1034538314
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/0092-8674(82)90324-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050794369
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/0092-8674(82)90400-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012378105
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/0092-8674(90)90696-c schema:sameAs https://app.dimensions.ai/details/publication/pub.1041327415
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/0092-8674(93)90251-k schema:sameAs https://app.dimensions.ai/details/publication/pub.1031600813
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/0092-8674(94)90383-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012133473
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/0165-2478(91)90220-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021762448
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/0190-9622(90)70197-p schema:sameAs https://app.dimensions.ai/details/publication/pub.1026208543
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/0190-9622(91)70015-t schema:sameAs https://app.dimensions.ai/details/publication/pub.1024234633
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/0190-9622(91)70188-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024578573
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/b978-0-12-505263-4.50012-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025367999
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/s0002-9610(99)80258-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1046728298
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/s0140-6736(81)90006-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027678149
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/s0190-9622(86)70048-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052294892
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/s0190-9622(87)70199-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046693939
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/s0190-9622(87)70263-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033436731
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/s0190-9622(89)70160-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025994533
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1083/jcb.122.2.417 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033701108
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1084/jem.156.6.1755 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008388622
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1097/00000637-199102000-00015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050477884
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1097/00006534-198907000-00001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006092701
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1097/00007890-199504150-00020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044512380
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1111/j.1365-2133.1981.tb01704.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1028947010
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1111/j.1365-2133.1987.tb07491.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1008050690
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1111/j.1365-2133.1990.tb01844.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009509343
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1111/j.1365-2230.1986.tb00526.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1007509526
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1111/j.1365-2230.1993.tb02240.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022406046
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1111/j.1432-0436.1986.tb00395.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022830756
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1111/j.1432-0436.1987.tb00174.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1046314841
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1111/j.1524-4725.1988.tb03343.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006555064
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1111/j.1524-4725.1994.tb03715.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1027276932
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1111/j.1600-0625.1993.tb00033.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1012717291
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1111/j.1749-6632.1991.tb24385.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1051385654
283 rdf:type schema:CreativeWork
284 https://www.grid.ac/institutes/grid.5734.5 schema:alternateName University of Bern
285 schema:name Dermatologic Clinic, University of Bern, Switzerland.
286 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...