A Tissue Fixative that Protects Macromolecules (DNA, RNA, and Protein) and Histomorphology in Clinical Samples View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2003-10

AUTHORS

Vladimir Vincek, Mehdi Nassiri, Mehrdad Nadji, Azorides R Morales

ABSTRACT

Preservation of macromolecules (DNA, RNA, and proteins) in tissue is traditionally achieved by immediate freezing of the sample. Although isolation of PCR-able RNA has been reported from formalin-fixed, paraffin-embedded tissues, the process has not been shown to be reproducible because high molecular weight RNA is usually degraded. We investigated the potential value of a new universal molecular fixative (UMFIX, Sakura Finetek USA, Inc., Torrance, California) in preservation of macromolecules in paraffin-embedded tissue. Mouse and human tissues were fixed in UMFIX from 1 hour to 8 weeks. They were then processed by a rapid tissue processing (RTP) system, embedded in paraffin, and evaluated for routine histology as well as for the quality and quantity of DNA, RNA, and proteins. Formalin-fixed tissues were processed by RTP and evaluated in a similar manner. Fresh-frozen samples were used as controls. The morphology of UMFIX-exposed tissue was comparable to that fixed in formalin. High molecular weight RNA was preserved in tissue that was immediately fixed in UMFIX and stored from 1 hour to 8 weeks at room temperature. There were no significant differences between UMFIX-exposed and frozen tissues on PCR, RT-PCR, real-time PCR, and expression microarrays. Similarly, physical and antigenic preservation of proteins in UMFIX tissue was similar to fresh state. Both RNA and proteins were substantially degraded in formalin-fixed and similarly processed specimens. We concluded that it is now possible to preserve histomorphology and intact macromolecules in the same archival paraffin-embedded tissue through the use of a novel fixative and a rapid processing system. More... »

PAGES

1427

Identifiers

URI

http://scigraph.springernature.com/pub.10.1097/01.lab.0000090154.55436.d1

DOI

http://dx.doi.org/10.1097/01.lab.0000090154.55436.d1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015864419

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/14563944


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Artifacts", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fixatives", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Liver", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred C57BL", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oligonucleotide Array Sequence Analysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Paraffin Embedding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pathology, Clinical", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reproducibility of Results", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reverse Transcriptase Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tissue Fixation", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Miami", 
          "id": "https://www.grid.ac/institutes/grid.26790.3a", 
          "name": [
            "Department of Pathology, Jackson Memorial Medical Center, University of Miami, Miami, Florida"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vincek", 
        "givenName": "Vladimir", 
        "id": "sg:person.0776675366.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776675366.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Miami", 
          "id": "https://www.grid.ac/institutes/grid.26790.3a", 
          "name": [
            "Department of Pathology, Jackson Memorial Medical Center, University of Miami, Miami, Florida"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nassiri", 
        "givenName": "Mehdi", 
        "id": "sg:person.0603417126.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603417126.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Miami", 
          "id": "https://www.grid.ac/institutes/grid.26790.3a", 
          "name": [
            "Department of Pathology, Jackson Memorial Medical Center, University of Miami, Miami, Florida"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nadji", 
        "givenName": "Mehrdad", 
        "id": "sg:person.01101263473.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101263473.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Miami", 
          "id": "https://www.grid.ac/institutes/grid.26790.3a", 
          "name": [
            "Department of Pathology, Jackson Memorial Medical Center, University of Miami, Miami, Florida"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Morales", 
        "givenName": "Azorides R", 
        "id": "sg:person.0766136747.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766136747.38"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/1096-9896(200109)195:1<66::aid-path921>3.0.co;2-f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002434228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1159/000049777", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006492380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/modpathol.3880267", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007062157", 
          "https://doi.org/10.1038/modpathol.3880267"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/modpathol.3880267", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007062157", 
          "https://doi.org/10.1038/modpathol.3880267"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0002-9440(10)64864-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009297361"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0002-9440(10)63985-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010647402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/27.22.4436", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019725071"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1440-1827.1997.tb04442.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022789548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00000372-199510000-00008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028353638"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00000372-199510000-00008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028353638"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00019606-199808000-00004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036124093"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00019606-199808000-00004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036124093"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1096-9896(200109)195:1<31::aid-path920>3.0.co;2-w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039509066"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1096-9896(200109)195:1<1::aid-path922>3.0.co;2-g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048029344"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.3780023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049101021", 
          "https://doi.org/10.1038/labinvest.3780023"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.3780023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049101021", 
          "https://doi.org/10.1038/labinvest.3780023"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.889", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051466685"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.1711630114", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051588226"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/path.1711630114", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051588226"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0272-2712(18)30092-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074554341"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075035250", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2003-10", 
    "datePublishedReg": "2003-10-01", 
    "description": "Preservation of macromolecules (DNA, RNA, and proteins) in tissue is traditionally achieved by immediate freezing of the sample. Although isolation of PCR-able RNA has been reported from formalin-fixed, paraffin-embedded tissues, the process has not been shown to be reproducible because high molecular weight RNA is usually degraded. We investigated the potential value of a new universal molecular fixative (UMFIX, Sakura Finetek USA, Inc., Torrance, California) in preservation of macromolecules in paraffin-embedded tissue. Mouse and human tissues were fixed in UMFIX from 1 hour to 8 weeks. They were then processed by a rapid tissue processing (RTP) system, embedded in paraffin, and evaluated for routine histology as well as for the quality and quantity of DNA, RNA, and proteins. Formalin-fixed tissues were processed by RTP and evaluated in a similar manner. Fresh-frozen samples were used as controls. The morphology of UMFIX-exposed tissue was comparable to that fixed in formalin. High molecular weight RNA was preserved in tissue that was immediately fixed in UMFIX and stored from 1 hour to 8 weeks at room temperature. There were no significant differences between UMFIX-exposed and frozen tissues on PCR, RT-PCR, real-time PCR, and expression microarrays. Similarly, physical and antigenic preservation of proteins in UMFIX tissue was similar to fresh state. Both RNA and proteins were substantially degraded in formalin-fixed and similarly processed specimens. We concluded that it is now possible to preserve histomorphology and intact macromolecules in the same archival paraffin-embedded tissue through the use of a novel fixative and a rapid processing system.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1097/01.lab.0000090154.55436.d1", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1017964", 
        "issn": [
          "0023-6837", 
          "1530-0307"
        ], 
        "name": "Laboratory Investigation", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "83"
      }
    ], 
    "name": "A Tissue Fixative that Protects Macromolecules (DNA, RNA, and Protein) and Histomorphology in Clinical Samples", 
    "pagination": "1427", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "4b9735e8c404eea371d7f91b2932b2972434ce0b0913e64388260d5943b9f194"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "14563944"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0376617"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1097/01.lab.0000090154.55436.d1"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1015864419"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1097/01.lab.0000090154.55436.d1", 
      "https://app.dimensions.ai/details/publication/pub.1015864419"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T16:50", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000556.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/3780733"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1097/01.lab.0000090154.55436.d1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1097/01.lab.0000090154.55436.d1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1097/01.lab.0000090154.55436.d1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1097/01.lab.0000090154.55436.d1'


 

This table displays all metadata directly associated to this object as RDF triples.

211 TRIPLES      21 PREDICATES      63 URIs      39 LITERALS      27 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1097/01.lab.0000090154.55436.d1 schema:about N08bca4629aef4750818b22859839583f
2 N1c1fb0ebe5d34758b42a794894bbb0fd
3 N357673d5b9ed412999d490a8c88e98eb
4 N537c3a77eb6b48148c3851f617d09cc4
5 N5534d5b111e343bf9fbb0c24a9688be2
6 N57e4280816384b3da06644e832c80f99
7 N6eebe3de935d4627b0da13703ae5073e
8 N844c62f2622c4a709a64d1fd3c1a55ad
9 N88f9c83ea15f4919863815bb7fd13989
10 N8b987b9be5444178bbd0c87edc4d1a9e
11 N8c8aedf4dd63494296d22a17d9a0fc04
12 Na2fcd202d7ad436bbc4db31ee244780c
13 Na3ee4298bc5d453f8fe385245ce02eb6
14 Nb48be301e7a94891bc4c49ba293f7c43
15 Ndceee84835ab4cbcb54006ec7fb7c7c9
16 Ne809be8460d54dbaa34ca5e0ca2c9a5d
17 Nea0d00acd2f34895bf4f3b2e1f5589a0
18 Nffc9aeddd9bf419ebc301c13ab12c229
19 anzsrc-for:06
20 anzsrc-for:0604
21 schema:author N2b427ac60f73460da182104e55236713
22 schema:citation sg:pub.10.1038/labinvest.3780023
23 sg:pub.10.1038/modpathol.3880267
24 https://app.dimensions.ai/details/publication/pub.1075035250
25 https://doi.org/10.1002/1096-9896(200109)195:1<1::aid-path922>3.0.co;2-g
26 https://doi.org/10.1002/1096-9896(200109)195:1<31::aid-path920>3.0.co;2-w
27 https://doi.org/10.1002/1096-9896(200109)195:1<66::aid-path921>3.0.co;2-f
28 https://doi.org/10.1002/path.1711630114
29 https://doi.org/10.1002/path.889
30 https://doi.org/10.1016/s0002-9440(10)63985-5
31 https://doi.org/10.1016/s0002-9440(10)64864-x
32 https://doi.org/10.1016/s0272-2712(18)30092-1
33 https://doi.org/10.1093/nar/27.22.4436
34 https://doi.org/10.1097/00000372-199510000-00008
35 https://doi.org/10.1097/00019606-199808000-00004
36 https://doi.org/10.1111/j.1440-1827.1997.tb04442.x
37 https://doi.org/10.1159/000049777
38 schema:datePublished 2003-10
39 schema:datePublishedReg 2003-10-01
40 schema:description Preservation of macromolecules (DNA, RNA, and proteins) in tissue is traditionally achieved by immediate freezing of the sample. Although isolation of PCR-able RNA has been reported from formalin-fixed, paraffin-embedded tissues, the process has not been shown to be reproducible because high molecular weight RNA is usually degraded. We investigated the potential value of a new universal molecular fixative (UMFIX, Sakura Finetek USA, Inc., Torrance, California) in preservation of macromolecules in paraffin-embedded tissue. Mouse and human tissues were fixed in UMFIX from 1 hour to 8 weeks. They were then processed by a rapid tissue processing (RTP) system, embedded in paraffin, and evaluated for routine histology as well as for the quality and quantity of DNA, RNA, and proteins. Formalin-fixed tissues were processed by RTP and evaluated in a similar manner. Fresh-frozen samples were used as controls. The morphology of UMFIX-exposed tissue was comparable to that fixed in formalin. High molecular weight RNA was preserved in tissue that was immediately fixed in UMFIX and stored from 1 hour to 8 weeks at room temperature. There were no significant differences between UMFIX-exposed and frozen tissues on PCR, RT-PCR, real-time PCR, and expression microarrays. Similarly, physical and antigenic preservation of proteins in UMFIX tissue was similar to fresh state. Both RNA and proteins were substantially degraded in formalin-fixed and similarly processed specimens. We concluded that it is now possible to preserve histomorphology and intact macromolecules in the same archival paraffin-embedded tissue through the use of a novel fixative and a rapid processing system.
41 schema:genre research_article
42 schema:inLanguage en
43 schema:isAccessibleForFree true
44 schema:isPartOf Nd953300cc3fa4df79e6633f3f193dbdf
45 Nf87c7e4b50df4c5ca06ed03ac2e20e86
46 sg:journal.1017964
47 schema:name A Tissue Fixative that Protects Macromolecules (DNA, RNA, and Protein) and Histomorphology in Clinical Samples
48 schema:pagination 1427
49 schema:productId N31727fab13764fa388830b5c30966116
50 N6f718107d92a40b5a887f9e21f74504b
51 N93afc15b971a4a6085724741ea25d66e
52 Na933c51338ea40f4bf0dcbb3d0e73ef6
53 Nceaa98357a184e808bc0bab19ccc1a89
54 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015864419
55 https://doi.org/10.1097/01.lab.0000090154.55436.d1
56 schema:sdDatePublished 2019-04-10T16:50
57 schema:sdLicense https://scigraph.springernature.com/explorer/license/
58 schema:sdPublisher N62a9bac018dd44ed8f5834ea5cf42e81
59 schema:url https://www.nature.com/articles/3780733
60 sgo:license sg:explorer/license/
61 sgo:sdDataset articles
62 rdf:type schema:ScholarlyArticle
63 N08bca4629aef4750818b22859839583f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
64 schema:name Mice
65 rdf:type schema:DefinedTerm
66 N1c1fb0ebe5d34758b42a794894bbb0fd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
67 schema:name Animals
68 rdf:type schema:DefinedTerm
69 N2b427ac60f73460da182104e55236713 rdf:first sg:person.0776675366.33
70 rdf:rest N71076b1d7c954d54a041324a3595d988
71 N31727fab13764fa388830b5c30966116 schema:name pubmed_id
72 schema:value 14563944
73 rdf:type schema:PropertyValue
74 N357673d5b9ed412999d490a8c88e98eb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Mice, Inbred C57BL
76 rdf:type schema:DefinedTerm
77 N3f1ac4082a2146178591978182ab1da1 rdf:first sg:person.0766136747.38
78 rdf:rest rdf:nil
79 N537c3a77eb6b48148c3851f617d09cc4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Reverse Transcriptase Polymerase Chain Reaction
81 rdf:type schema:DefinedTerm
82 N5534d5b111e343bf9fbb0c24a9688be2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Pathology, Clinical
84 rdf:type schema:DefinedTerm
85 N57e4280816384b3da06644e832c80f99 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Artifacts
87 rdf:type schema:DefinedTerm
88 N5f6130ec98c04ccea46307858565b701 rdf:first sg:person.01101263473.14
89 rdf:rest N3f1ac4082a2146178591978182ab1da1
90 N62a9bac018dd44ed8f5834ea5cf42e81 schema:name Springer Nature - SN SciGraph project
91 rdf:type schema:Organization
92 N6eebe3de935d4627b0da13703ae5073e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Reproducibility of Results
94 rdf:type schema:DefinedTerm
95 N6f718107d92a40b5a887f9e21f74504b schema:name doi
96 schema:value 10.1097/01.lab.0000090154.55436.d1
97 rdf:type schema:PropertyValue
98 N71076b1d7c954d54a041324a3595d988 rdf:first sg:person.0603417126.83
99 rdf:rest N5f6130ec98c04ccea46307858565b701
100 N844c62f2622c4a709a64d1fd3c1a55ad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Male
102 rdf:type schema:DefinedTerm
103 N88f9c83ea15f4919863815bb7fd13989 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name RNA
105 rdf:type schema:DefinedTerm
106 N8b987b9be5444178bbd0c87edc4d1a9e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Humans
108 rdf:type schema:DefinedTerm
109 N8c8aedf4dd63494296d22a17d9a0fc04 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name DNA
111 rdf:type schema:DefinedTerm
112 N93afc15b971a4a6085724741ea25d66e schema:name nlm_unique_id
113 schema:value 0376617
114 rdf:type schema:PropertyValue
115 Na2fcd202d7ad436bbc4db31ee244780c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Tissue Fixation
117 rdf:type schema:DefinedTerm
118 Na3ee4298bc5d453f8fe385245ce02eb6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Proteins
120 rdf:type schema:DefinedTerm
121 Na933c51338ea40f4bf0dcbb3d0e73ef6 schema:name readcube_id
122 schema:value 4b9735e8c404eea371d7f91b2932b2972434ce0b0913e64388260d5943b9f194
123 rdf:type schema:PropertyValue
124 Nb48be301e7a94891bc4c49ba293f7c43 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Liver
126 rdf:type schema:DefinedTerm
127 Nceaa98357a184e808bc0bab19ccc1a89 schema:name dimensions_id
128 schema:value pub.1015864419
129 rdf:type schema:PropertyValue
130 Nd953300cc3fa4df79e6633f3f193dbdf schema:issueNumber 10
131 rdf:type schema:PublicationIssue
132 Ndceee84835ab4cbcb54006ec7fb7c7c9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Oligonucleotide Array Sequence Analysis
134 rdf:type schema:DefinedTerm
135 Ne809be8460d54dbaa34ca5e0ca2c9a5d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Paraffin Embedding
137 rdf:type schema:DefinedTerm
138 Nea0d00acd2f34895bf4f3b2e1f5589a0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Fixatives
140 rdf:type schema:DefinedTerm
141 Nf87c7e4b50df4c5ca06ed03ac2e20e86 schema:volumeNumber 83
142 rdf:type schema:PublicationVolume
143 Nffc9aeddd9bf419ebc301c13ab12c229 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Female
145 rdf:type schema:DefinedTerm
146 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
147 schema:name Biological Sciences
148 rdf:type schema:DefinedTerm
149 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
150 schema:name Genetics
151 rdf:type schema:DefinedTerm
152 sg:journal.1017964 schema:issn 0023-6837
153 1530-0307
154 schema:name Laboratory Investigation
155 rdf:type schema:Periodical
156 sg:person.01101263473.14 schema:affiliation https://www.grid.ac/institutes/grid.26790.3a
157 schema:familyName Nadji
158 schema:givenName Mehrdad
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101263473.14
160 rdf:type schema:Person
161 sg:person.0603417126.83 schema:affiliation https://www.grid.ac/institutes/grid.26790.3a
162 schema:familyName Nassiri
163 schema:givenName Mehdi
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603417126.83
165 rdf:type schema:Person
166 sg:person.0766136747.38 schema:affiliation https://www.grid.ac/institutes/grid.26790.3a
167 schema:familyName Morales
168 schema:givenName Azorides R
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766136747.38
170 rdf:type schema:Person
171 sg:person.0776675366.33 schema:affiliation https://www.grid.ac/institutes/grid.26790.3a
172 schema:familyName Vincek
173 schema:givenName Vladimir
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776675366.33
175 rdf:type schema:Person
176 sg:pub.10.1038/labinvest.3780023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049101021
177 https://doi.org/10.1038/labinvest.3780023
178 rdf:type schema:CreativeWork
179 sg:pub.10.1038/modpathol.3880267 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007062157
180 https://doi.org/10.1038/modpathol.3880267
181 rdf:type schema:CreativeWork
182 https://app.dimensions.ai/details/publication/pub.1075035250 schema:CreativeWork
183 https://doi.org/10.1002/1096-9896(200109)195:1<1::aid-path922>3.0.co;2-g schema:sameAs https://app.dimensions.ai/details/publication/pub.1048029344
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1002/1096-9896(200109)195:1<31::aid-path920>3.0.co;2-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1039509066
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1002/1096-9896(200109)195:1<66::aid-path921>3.0.co;2-f schema:sameAs https://app.dimensions.ai/details/publication/pub.1002434228
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1002/path.1711630114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051588226
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1002/path.889 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051466685
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/s0002-9440(10)63985-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010647402
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/s0002-9440(10)64864-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009297361
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/s0272-2712(18)30092-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074554341
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1093/nar/27.22.4436 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019725071
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1097/00000372-199510000-00008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028353638
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1097/00019606-199808000-00004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036124093
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1111/j.1440-1827.1997.tb04442.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022789548
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1159/000049777 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006492380
208 rdf:type schema:CreativeWork
209 https://www.grid.ac/institutes/grid.26790.3a schema:alternateName University of Miami
210 schema:name Department of Pathology, Jackson Memorial Medical Center, University of Miami, Miami, Florida
211 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...