Dystroglycan Expression in Hepatic Stellate Cells: Role in Liver Fibrosis View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2002-08

AUTHORS

Pierre Bedossa, Sophie Ferlicot, Valérie Paradis, Delphine Dargère, Frank Bonvoust, Michel Vidaud

ABSTRACT

Dystroglycan is a membrane component of the dystrophin-glycoprotein transmembrane complex. Its expression is required for the spatial organization of laminin on the cell surface and for basement membrane assembly. In view of the constitution of a perisinusoidal basement membrane during liver fibrosis, we studied dystroglycan expression in liver fibrosis. Dystroglycan mRNA and protein expression were investigated by immunofluorescence, Western blot, and quantitative RT-PCR (TaqMan) in normal human and rat liver and in isolated rat hepatic stellate cells. On Western blot, a 43-kd band corresponding to beta-dystroglycan was observed in protein extracted from normal and fibrotic human and rat livers. The specific 43-kd protein was also detected in lysates from rat hepatic stellate cells but not from hepatocytes. By immunofluorescence, patchy deposits of beta-dystroglycan were detected on membrane of hepatic stellate cells in culture. On Western blot and quantitative RT-PCR, an up-regulation of beta-dystroglycan was shown during spontaneous activation of hepatic stellate cells in culture. Direct evidence for the role of dystroglycan in laminin-hepatic stellate cell interaction was shown because specific antibody directed against alpha-dystroglycan inhibited partially hepatic stellate cell adhesion on laminin-coated plates. This mechanism was calcium dependent because EDTA inhibited cell/laminin adhesion, an effect reversed by addition of Ca(2+). This study shows that dystroglycan is expressed on the membrane of hepatic stellate cells and is up-regulated during liver fibrosis. Dystroglycan interaction with laminin should be implicated in the concentration of pericellular laminin and in the constitution of a perisinusoidal basement membrane during liver fibrosis. More... »

PAGES

3780507

Identifiers

URI

http://scigraph.springernature.com/pub.10.1097/01.lab.0000024429.73158.de

DOI

http://dx.doi.org/10.1097/01.lab.0000024429.73158.de

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046976103

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12177244


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curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1097/01.lab.0000024429.73158.de'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1097/01.lab.0000024429.73158.de'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1097/01.lab.0000024429.73158.de'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1097/01.lab.0000024429.73158.de'


 

This table displays all metadata directly associated to this object as RDF triples.

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