In vitro regeneration of Echinacea purpurea L.: Enhancement of somatic embryogenesis by indolebutyric acid and dark pre-incubation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-11

AUTHORS

S. M. A. Zobayed, P. K. Saxena

ABSTRACT

The embryogenic potential of different Echinacea purpurea tissues, viz. leaf, cotyledon, and root, was investigated. Maximum embryo-induction was achieved from leaf dises cultured on Murashige and Skoog medium supplemented with benzylaminopurine (5.0 μM) and indolebutyric acid (2.5 μM) where 95% of the explants responded, yielding an average of 83 embryos per explant within 4 wk of culture. Incubation of cultures in the dark for an initial period of 14 d significantly increased the frequency of somatic embryogenesis (6–8-fold in leaf explants). Exposure of the abaxial surface of leaves to the medium significantly increased the number of embryos. Transfer of somatic embryos to a medium devoid of growth regulators resulted in 80% germination within 7 d. Over 73% of the somatic embryos developed roots within 28 d of culture on a medium containing naphthaleneacetic acid (10 μM) with a maximum root number of 9.8 per plantlet. Transplanting ex vitro and acclimatization for a period of 7 d were sufficient to promote establishment of plants in the greenhouse, and more than 90% of the regenerated plants survived. More... »

PAGES

605-612

References to SciGraph publications

  • 1993-05. Thidiazuron: a potent cytokinin for woody plant tissue culture in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2002-03. Somatic embryogenesis and plant regeneration from immature embryos of saw palmetto, an important landscape and medicinal plant in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2002-04. In vitro regeneration of Echinacea purpurea from leaf explants in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2000-01. In vitro regeneration of Echinacea purpurea L.: Direct somatic embryogenesis and indirect shoot organogenesis in petiole culture in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 2000-01. Light Mediates Endogenous Plant Growth Substances in Thidiazuron-induced Somatic Embryogenesis in Geranium Hypocotyl Cultures in JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1999. Chemistry, analysis and immunological investigations of Echinacea phytopharmaceuticals in IMMUNOMODULATORY AGENTS FROM PLANTS
  • 2000-09. Preface in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1993-04. The use of thidiazuron in tissue culture in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 1998-10. Thidiazuron: A potent regulator ofin vitro plant morphogenesis in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1079/ivp2003434

    DOI

    http://dx.doi.org/10.1079/ivp2003434

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024434459


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