Optimisation of culture conditions for in vitro infection of tomato with the root-knot nematode Meloidogyne javanica View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-06

AUTHORS

P. Hutangura, M. G. K. Jones, T. Heinrich

ABSTRACT

Conditions for in vitro culture of tomato seedlings (Lycopersicon esculentum) were optimised to yield high in vitro rates of infection by the root-knot nematode Meloidogyne javanica. The influence of nutrient salts, gelling agents, sucrose concentration and pH on the development of nematode-induced root galis was investigated. A quarter-strength Murashige and Skoog medium supplemented by 0.5% sucrose and solidified with 0.6% phytagel at pH 6.4 gave most galis on tomato roots. Eggs were sterilised in 2.5% sodium hypochlorite for 4 min followed by 0.2% mercuric chloride for 4 min. Surface-sterilised eggs showed a 16% cumulative hatching rate within 10 days. Tomato seedlings cultivated in vitro for 1 week were inoculated with sterile eggs and the infection process was monitored weekly. After 5 to 7 weeks, sterile egg masses were harvested, second-stage juveniles were hatched in sterile distilled water and used to re-inoculate tomato seedlings without further sterilisation. The culture conditions described gave similar infection results for the related root-knot nematodes M. incognita, M. arenaria and M. hapla. More... »

PAGES

84-89

Journal

TITLE

Australasian Plant Pathology

ISSUE

2

VOLUME

27

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1071/ap98010

DOI

http://dx.doi.org/10.1071/ap98010

DIMENSIONS

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