Inhibition of heparin-binding epidermal growth factor-like growth factor increases albuminuria in puromycin aminonucleoside nephrosis View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2000-09

AUTHORS

T F Khong, S Fraser, M Katerelos, K Paizis, P A Hill, D A Power

ABSTRACT

BACKGROUND: Our previous work in the acute puromycin aminonucleoside nephrosis (PAN) model has demonstrated up-regulation of heparin-binding epidermal growth factor-like growth factor (HB-EGF) mRNA and protein within glomerular epithelial cells (GECs) prior to the onset of proteinuria. METHODS: To determine whether increased HB-EGF expression in the acute PAN model contributes to the pathogenesis of proteinuria, a monoclonal antibody (DE10) was produced against recombinant human HB-EGF. RESULTS: The specificity of DE10 for human HB-EGF was confirmed by enzyme-linked immunosorbent assay, immunohistochemical staining, and flow cytometry of transfected cells expressing human and rat HB-EGF, and inhibition of cell proliferation. DE10 also reacted with cells transfected with rat HB-EGF cDNA. Administration of 0.5 mg affinity-purified DE10 to normal rats did not cause significant albuminuria compared with controls. Five days after the induction of the acute PAN model, albuminuria was significantly greater in animals treated with 0.5 mg DE10 than a control mAb (162.6 +/- 32.4 vs. 64.8 +/- 10.2 mg/day, respectively, P < 0.01). Rats treated with DE10 had an earlier onset of severe albuminuria, but no increase in maximal albuminuria at later time points. Electron microscopy showed marked podocyte effacement in both DE10-treated and control animals, but no obvious difference between groups. However, adhesion of the human GEC line 56/10 A1 to laminin and fibronectin, but not to collagens I or IV, was reduced by DE10. CONCLUSIONS: This study suggests that HB-EGF contributes to the integrity of the glomerular filtration barrier, particularly when the podocyte has been injured. Following podocyte injury, adhesion to laminin in the glomerular basement membrane by HB-EGF may be important in reducing albuminuria. More... »

PAGES

1098-1107

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1046/j.1523-1755.2000.00267.x

DOI

http://dx.doi.org/10.1046/j.1523-1755.2000.00267.x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026715967

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10972674


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "3T3 Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Actins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Albuminuria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibiotics, Antineoplastic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Monoclonal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibody Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CHO Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "COS Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Adhesion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cricetinae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epidermal Growth Factor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flow Cytometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glomerular Filtration Rate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Heparin-binding EGF-like Growth Factor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Integrin alpha3beta1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Integrins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Intercellular Signaling Peptides and Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kidney", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred BALB C", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Confocal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Electron", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nephrosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neutralization Tests", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Puromycin Aminonucleoside", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "St Vincent's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.413105.2", 
          "name": [
            "Immunology Research Center and the Department of Anatomical Pathology, St. Vincent's Hospital, Fitzroy, Victoria, Australia."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Khong", 
        "givenName": "T F", 
        "id": "sg:person.01335455776.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335455776.72"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Fraser", 
        "givenName": "S", 
        "id": "sg:person.01053227266.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01053227266.83"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Katerelos", 
        "givenName": "M", 
        "id": "sg:person.01121342466.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01121342466.28"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Paizis", 
        "givenName": "K", 
        "id": "sg:person.01120617545.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01120617545.34"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Hill", 
        "givenName": "P A", 
        "id": "sg:person.01366067302.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366067302.35"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Power", 
        "givenName": "D A", 
        "id": "sg:person.01050643265.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050643265.26"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1083/jcb.129.6.1691", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000066738"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.49.31036", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000821988"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci118087", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019526766"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci112195", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022201302"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.175.3.623", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022868307"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ki.1991.292", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035046471", 
          "https://doi.org/10.1038/ki.1991.292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0019-2791(71)90454-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037949893"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0019-2791(71)90454-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037949893"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ki.1975.105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041896677", 
          "https://doi.org/10.1038/ki.1975.105"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0304-419x(97)00024-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042389470"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci119386", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043332173"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1046/j.1523-1755.1998.00846.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048426004", 
          "https://doi.org/10.1046/j.1523-1755.1998.00846.x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0161-5890(71)90063-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054552919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0161-5890(71)90063-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054552919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajprenal.1992.262.5.f697", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077054659"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajprenal.1992.262.5.f770", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077054668"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078449340", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079723092", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079973751", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083011386", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083343820", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2000-09", 
    "datePublishedReg": "2000-09-01", 
    "description": "BACKGROUND: Our previous work in the acute puromycin aminonucleoside nephrosis (PAN) model has demonstrated up-regulation of heparin-binding epidermal growth factor-like growth factor (HB-EGF) mRNA and protein within glomerular epithelial cells (GECs) prior to the onset of proteinuria.\nMETHODS: To determine whether increased HB-EGF expression in the acute PAN model contributes to the pathogenesis of proteinuria, a monoclonal antibody (DE10) was produced against recombinant human HB-EGF.\nRESULTS: The specificity of DE10 for human HB-EGF was confirmed by enzyme-linked immunosorbent assay, immunohistochemical staining, and flow cytometry of transfected cells expressing human and rat HB-EGF, and inhibition of cell proliferation. DE10 also reacted with cells transfected with rat HB-EGF cDNA. Administration of 0.5 mg affinity-purified DE10 to normal rats did not cause significant albuminuria compared with controls. Five days after the induction of the acute PAN model, albuminuria was significantly greater in animals treated with 0.5 mg DE10 than a control mAb (162.6 +/- 32.4 vs. 64.8 +/- 10.2 mg/day, respectively, P < 0.01). Rats treated with DE10 had an earlier onset of severe albuminuria, but no increase in maximal albuminuria at later time points. Electron microscopy showed marked podocyte effacement in both DE10-treated and control animals, but no obvious difference between groups. However, adhesion of the human GEC line 56/10 A1 to laminin and fibronectin, but not to collagens I or IV, was reduced by DE10.\nCONCLUSIONS: This study suggests that HB-EGF contributes to the integrity of the glomerular filtration barrier, particularly when the podocyte has been injured. Following podocyte injury, adhesion to laminin in the glomerular basement membrane by HB-EGF may be important in reducing albuminuria.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1046/j.1523-1755.2000.00267.x", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1015319", 
        "issn": [
          "0085-2538", 
          "1523-1755"
        ], 
        "name": "Kidney International", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "58"
      }
    ], 
    "name": "Inhibition of heparin-binding epidermal growth factor-like growth factor increases albuminuria in puromycin aminonucleoside nephrosis", 
    "pagination": "1098-1107", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "7f45ce86b0c2f1abb97c54203018857cdf59cc55384331fb70acf145e20a5ec3"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10972674"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0323470"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1046/j.1523-1755.2000.00267.x"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1026715967"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1046/j.1523-1755.2000.00267.x", 
      "https://app.dimensions.ai/details/publication/pub.1026715967"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T13:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000424.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/ki/journal/v58/n3/full/4491787a.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1046/j.1523-1755.2000.00267.x'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1046/j.1523-1755.2000.00267.x'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1046/j.1523-1755.2000.00267.x'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1046/j.1523-1755.2000.00267.x'


 

This table displays all metadata directly associated to this object as RDF triples.

274 TRIPLES      21 PREDICATES      78 URIs      51 LITERALS      39 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1046/j.1523-1755.2000.00267.x schema:about N016ffc061c894183a9a17848d68d8563
2 N03008018cdf34055a1764069b5ffa93d
3 N045d77dd5dcb409bb8bc4c63ab165e73
4 N06a14cfd7d5a4352b8339e3396dca039
5 N0db8668209f24fd4ac5fa64d1aac7fec
6 N0fbf01e92cc7420d8eb3daf145484a4c
7 N147cdaea74104b2394eedef94913a315
8 N176d53c0a7a14e39b57b5a48248d265b
9 N206e746fde044b2a8f9ecaf7220a9c4b
10 N237a1dbb76104cf0aa4122e4f877a798
11 N2f35db78e4964cc780cb506bcde38f38
12 N31503b69e126490298b9e1af1a64ad12
13 N435d74c35e7d4bb58a7f63d4b2dcd07c
14 N4980e30a1e7f4d13a17cae15518f71f2
15 N53dc9cea34cd4842906dc3d0396083c6
16 N56510b9b4cae44b49ed9ac3b96fbc827
17 N607d8ddd6da94153bcacccbe001f0b1b
18 N610f82b07c994f5b8aa4ce40e83761b3
19 N62d31f8febc44f5393052c6408ab232e
20 N6da7030ff4484c14b19c2f74f4ede66e
21 N75283e7746c04ed9a59bb2b363a27855
22 N85400f8b93774cd6a48001477ade0d0f
23 N8e8b55583c8d4236bfcc321c7f58dbf7
24 Na4e7e058bf164e37b8832717e9a62e7c
25 Nc30838d6ab79435c8027aeefcea294d0
26 Ne5df2c753397474fa69b0b4b9df29f0f
27 Ne5ecaf63641a415b8da842fa6ab619e3
28 Neba73dac742b4fef9e30361b82cda14a
29 Nf1d4d9777cec424887a374a7d8391b72
30 Nfb447fa037524d859891ec53ddcb842e
31 anzsrc-for:11
32 anzsrc-for:1103
33 schema:author Naf6cd199dea442359d8562da8ae4a9e9
34 schema:citation sg:pub.10.1038/ki.1975.105
35 sg:pub.10.1038/ki.1991.292
36 sg:pub.10.1046/j.1523-1755.1998.00846.x
37 https://app.dimensions.ai/details/publication/pub.1078449340
38 https://app.dimensions.ai/details/publication/pub.1079723092
39 https://app.dimensions.ai/details/publication/pub.1079973751
40 https://app.dimensions.ai/details/publication/pub.1083011386
41 https://app.dimensions.ai/details/publication/pub.1083343820
42 https://doi.org/10.1016/0019-2791(71)90454-x
43 https://doi.org/10.1016/0161-5890(71)90063-0
44 https://doi.org/10.1016/s0304-419x(97)00024-3
45 https://doi.org/10.1074/jbc.272.49.31036
46 https://doi.org/10.1083/jcb.129.6.1691
47 https://doi.org/10.1084/jem.175.3.623
48 https://doi.org/10.1152/ajprenal.1992.262.5.f697
49 https://doi.org/10.1152/ajprenal.1992.262.5.f770
50 https://doi.org/10.1172/jci112195
51 https://doi.org/10.1172/jci118087
52 https://doi.org/10.1172/jci119386
53 schema:datePublished 2000-09
54 schema:datePublishedReg 2000-09-01
55 schema:description BACKGROUND: Our previous work in the acute puromycin aminonucleoside nephrosis (PAN) model has demonstrated up-regulation of heparin-binding epidermal growth factor-like growth factor (HB-EGF) mRNA and protein within glomerular epithelial cells (GECs) prior to the onset of proteinuria. METHODS: To determine whether increased HB-EGF expression in the acute PAN model contributes to the pathogenesis of proteinuria, a monoclonal antibody (DE10) was produced against recombinant human HB-EGF. RESULTS: The specificity of DE10 for human HB-EGF was confirmed by enzyme-linked immunosorbent assay, immunohistochemical staining, and flow cytometry of transfected cells expressing human and rat HB-EGF, and inhibition of cell proliferation. DE10 also reacted with cells transfected with rat HB-EGF cDNA. Administration of 0.5 mg affinity-purified DE10 to normal rats did not cause significant albuminuria compared with controls. Five days after the induction of the acute PAN model, albuminuria was significantly greater in animals treated with 0.5 mg DE10 than a control mAb (162.6 +/- 32.4 vs. 64.8 +/- 10.2 mg/day, respectively, P < 0.01). Rats treated with DE10 had an earlier onset of severe albuminuria, but no increase in maximal albuminuria at later time points. Electron microscopy showed marked podocyte effacement in both DE10-treated and control animals, but no obvious difference between groups. However, adhesion of the human GEC line 56/10 A1 to laminin and fibronectin, but not to collagens I or IV, was reduced by DE10. CONCLUSIONS: This study suggests that HB-EGF contributes to the integrity of the glomerular filtration barrier, particularly when the podocyte has been injured. Following podocyte injury, adhesion to laminin in the glomerular basement membrane by HB-EGF may be important in reducing albuminuria.
56 schema:genre research_article
57 schema:inLanguage en
58 schema:isAccessibleForFree true
59 schema:isPartOf N3ce7451158104332877e7b2bbcc88a10
60 N7ecc438e7a5e4ee494156e829cda85c4
61 sg:journal.1015319
62 schema:name Inhibition of heparin-binding epidermal growth factor-like growth factor increases albuminuria in puromycin aminonucleoside nephrosis
63 schema:pagination 1098-1107
64 schema:productId N68880315be284a7987eead4682106428
65 N7332260919814806b8a5632a7977702f
66 N7d89a48b31dc414f87c70ff20b0247b1
67 N98867152b23c4cb7b19ba231e11f6839
68 Nf9d6d89690bd4c9293a3af225f916e17
69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026715967
70 https://doi.org/10.1046/j.1523-1755.2000.00267.x
71 schema:sdDatePublished 2019-04-10T13:55
72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
73 schema:sdPublisher N35a8411bc1c140e4a7077c4e9ff8a146
74 schema:url http://www.nature.com/ki/journal/v58/n3/full/4491787a.html
75 sgo:license sg:explorer/license/
76 sgo:sdDataset articles
77 rdf:type schema:ScholarlyArticle
78 N016ffc061c894183a9a17848d68d8563 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Nephrosis
80 rdf:type schema:DefinedTerm
81 N03008018cdf34055a1764069b5ffa93d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Rats
83 rdf:type schema:DefinedTerm
84 N045d77dd5dcb409bb8bc4c63ab165e73 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Animals
86 rdf:type schema:DefinedTerm
87 N06a14cfd7d5a4352b8339e3396dca039 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Flow Cytometry
89 rdf:type schema:DefinedTerm
90 N0db8668209f24fd4ac5fa64d1aac7fec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Intercellular Signaling Peptides and Proteins
92 rdf:type schema:DefinedTerm
93 N0fbf01e92cc7420d8eb3daf145484a4c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Actins
95 rdf:type schema:DefinedTerm
96 N147cdaea74104b2394eedef94913a315 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name CHO Cells
98 rdf:type schema:DefinedTerm
99 N176d53c0a7a14e39b57b5a48248d265b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Microscopy, Electron
101 rdf:type schema:DefinedTerm
102 N206e746fde044b2a8f9ecaf7220a9c4b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name COS Cells
104 rdf:type schema:DefinedTerm
105 N237a1dbb76104cf0aa4122e4f877a798 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Antibody Specificity
107 rdf:type schema:DefinedTerm
108 N2f35db78e4964cc780cb506bcde38f38 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Integrins
110 rdf:type schema:DefinedTerm
111 N31503b69e126490298b9e1af1a64ad12 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Neutralization Tests
113 rdf:type schema:DefinedTerm
114 N35a8411bc1c140e4a7077c4e9ff8a146 schema:name Springer Nature - SN SciGraph project
115 rdf:type schema:Organization
116 N3ce7451158104332877e7b2bbcc88a10 schema:issueNumber 3
117 rdf:type schema:PublicationIssue
118 N435d74c35e7d4bb58a7f63d4b2dcd07c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Mice, Inbred BALB C
120 rdf:type schema:DefinedTerm
121 N4980e30a1e7f4d13a17cae15518f71f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name 3T3 Cells
123 rdf:type schema:DefinedTerm
124 N53dc9cea34cd4842906dc3d0396083c6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Epidermal Growth Factor
126 rdf:type schema:DefinedTerm
127 N56510b9b4cae44b49ed9ac3b96fbc827 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Heparin-binding EGF-like Growth Factor
129 rdf:type schema:DefinedTerm
130 N607d8ddd6da94153bcacccbe001f0b1b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Microscopy, Confocal
132 rdf:type schema:DefinedTerm
133 N610f82b07c994f5b8aa4ce40e83761b3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Kidney
135 rdf:type schema:DefinedTerm
136 N62d31f8febc44f5393052c6408ab232e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Antibodies, Monoclonal
138 rdf:type schema:DefinedTerm
139 N68880315be284a7987eead4682106428 schema:name dimensions_id
140 schema:value pub.1026715967
141 rdf:type schema:PropertyValue
142 N6da7030ff4484c14b19c2f74f4ede66e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Cell Adhesion
144 rdf:type schema:DefinedTerm
145 N7332260919814806b8a5632a7977702f schema:name readcube_id
146 schema:value 7f45ce86b0c2f1abb97c54203018857cdf59cc55384331fb70acf145e20a5ec3
147 rdf:type schema:PropertyValue
148 N75283e7746c04ed9a59bb2b363a27855 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Glomerular Filtration Rate
150 rdf:type schema:DefinedTerm
151 N7d89a48b31dc414f87c70ff20b0247b1 schema:name doi
152 schema:value 10.1046/j.1523-1755.2000.00267.x
153 rdf:type schema:PropertyValue
154 N7ecc438e7a5e4ee494156e829cda85c4 schema:volumeNumber 58
155 rdf:type schema:PublicationVolume
156 N83d710589f454637a79433b122be4161 rdf:first sg:person.01366067302.35
157 rdf:rest Ndd7aa22247c24bd09cac3e439d04b8e3
158 N83e64f17c3d549ce99512e17d8d5ab6e rdf:first sg:person.01121342466.28
159 rdf:rest Nc8bc04d02de0459599174beb1feb7f2f
160 N85400f8b93774cd6a48001477ade0d0f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Albuminuria
162 rdf:type schema:DefinedTerm
163 N8e8b55583c8d4236bfcc321c7f58dbf7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Humans
165 rdf:type schema:DefinedTerm
166 N8f3259a742234573993a2d3a7f69a60a rdf:first sg:person.01053227266.83
167 rdf:rest N83e64f17c3d549ce99512e17d8d5ab6e
168 N98867152b23c4cb7b19ba231e11f6839 schema:name pubmed_id
169 schema:value 10972674
170 rdf:type schema:PropertyValue
171 Na4e7e058bf164e37b8832717e9a62e7c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Transfection
173 rdf:type schema:DefinedTerm
174 Naf6cd199dea442359d8562da8ae4a9e9 rdf:first sg:person.01335455776.72
175 rdf:rest N8f3259a742234573993a2d3a7f69a60a
176 Nc30838d6ab79435c8027aeefcea294d0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
177 schema:name Integrin alpha3beta1
178 rdf:type schema:DefinedTerm
179 Nc8bc04d02de0459599174beb1feb7f2f rdf:first sg:person.01120617545.34
180 rdf:rest N83d710589f454637a79433b122be4161
181 Ndd7aa22247c24bd09cac3e439d04b8e3 rdf:first sg:person.01050643265.26
182 rdf:rest rdf:nil
183 Ne5df2c753397474fa69b0b4b9df29f0f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Antibiotics, Antineoplastic
185 rdf:type schema:DefinedTerm
186 Ne5ecaf63641a415b8da842fa6ab619e3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
187 schema:name Puromycin Aminonucleoside
188 rdf:type schema:DefinedTerm
189 Neba73dac742b4fef9e30361b82cda14a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
190 schema:name Female
191 rdf:type schema:DefinedTerm
192 Nf1d4d9777cec424887a374a7d8391b72 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
193 schema:name Mice
194 rdf:type schema:DefinedTerm
195 Nf9d6d89690bd4c9293a3af225f916e17 schema:name nlm_unique_id
196 schema:value 0323470
197 rdf:type schema:PropertyValue
198 Nfb447fa037524d859891ec53ddcb842e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
199 schema:name Cricetinae
200 rdf:type schema:DefinedTerm
201 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
202 schema:name Medical and Health Sciences
203 rdf:type schema:DefinedTerm
204 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
205 schema:name Clinical Sciences
206 rdf:type schema:DefinedTerm
207 sg:journal.1015319 schema:issn 0085-2538
208 1523-1755
209 schema:name Kidney International
210 rdf:type schema:Periodical
211 sg:person.01050643265.26 schema:familyName Power
212 schema:givenName D A
213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050643265.26
214 rdf:type schema:Person
215 sg:person.01053227266.83 schema:familyName Fraser
216 schema:givenName S
217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01053227266.83
218 rdf:type schema:Person
219 sg:person.01120617545.34 schema:familyName Paizis
220 schema:givenName K
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01120617545.34
222 rdf:type schema:Person
223 sg:person.01121342466.28 schema:familyName Katerelos
224 schema:givenName M
225 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01121342466.28
226 rdf:type schema:Person
227 sg:person.01335455776.72 schema:affiliation https://www.grid.ac/institutes/grid.413105.2
228 schema:familyName Khong
229 schema:givenName T F
230 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335455776.72
231 rdf:type schema:Person
232 sg:person.01366067302.35 schema:familyName Hill
233 schema:givenName P A
234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366067302.35
235 rdf:type schema:Person
236 sg:pub.10.1038/ki.1975.105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041896677
237 https://doi.org/10.1038/ki.1975.105
238 rdf:type schema:CreativeWork
239 sg:pub.10.1038/ki.1991.292 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035046471
240 https://doi.org/10.1038/ki.1991.292
241 rdf:type schema:CreativeWork
242 sg:pub.10.1046/j.1523-1755.1998.00846.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1048426004
243 https://doi.org/10.1046/j.1523-1755.1998.00846.x
244 rdf:type schema:CreativeWork
245 https://app.dimensions.ai/details/publication/pub.1078449340 schema:CreativeWork
246 https://app.dimensions.ai/details/publication/pub.1079723092 schema:CreativeWork
247 https://app.dimensions.ai/details/publication/pub.1079973751 schema:CreativeWork
248 https://app.dimensions.ai/details/publication/pub.1083011386 schema:CreativeWork
249 https://app.dimensions.ai/details/publication/pub.1083343820 schema:CreativeWork
250 https://doi.org/10.1016/0019-2791(71)90454-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1037949893
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/0161-5890(71)90063-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054552919
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/s0304-419x(97)00024-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042389470
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1074/jbc.272.49.31036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000821988
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1083/jcb.129.6.1691 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000066738
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1084/jem.175.3.623 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022868307
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1152/ajprenal.1992.262.5.f697 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077054659
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1152/ajprenal.1992.262.5.f770 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077054668
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1172/jci112195 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022201302
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1172/jci118087 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019526766
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1172/jci119386 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043332173
271 rdf:type schema:CreativeWork
272 https://www.grid.ac/institutes/grid.413105.2 schema:alternateName St Vincent's Hospital
273 schema:name Immunology Research Center and the Department of Anatomical Pathology, St. Vincent's Hospital, Fitzroy, Victoria, Australia.
274 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...