Differential Expression of Connexins During Stratification of Human Keratinocytes View Full Text


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Article Info

DATE

2000-08

AUTHORS

Ludovic Wiszniewski, Alain Limat, Jean-Hilaire Saurat, Paolo Meda, Denis Salomon

ABSTRACT

To assess whether gap junctions and connexins change during keratinocyte differentiation, we have studied epidermal equivalents obtained in organotypic cultures of keratinocytes from the outer root sheath of human hair follicles. These reconstituted tissues exhibit a number of differentiation and proliferation markers of human epidermis, including gap junctions, connexins, and K6 and Ki67 proteins. Immunostaining and northern blots showed that gap junctions of the epidermal equivalents were made of Cx26 and Cx43. Cx26 was expressed in all keratinocyte layers, throughout the development of the epidermal equivalents. In contrast, Cx43 was initially observed only in the basal layer of keratinocytes and became detectable in the stratum spinosum and granulosum only after the epidermal equivalents had thickened. The levels of Cx26 and its transcript markedly increased as a function of stratification of the epidermal equivalents, whereas those of Cx43 remained almost constant. Microinjection of Lucifer Yellow into individual keratinocytes showed that gap junctions were similarly permeable at all stages of development of the epidermal equivalents. The data show that epidermal equivalents (i) feature a pattern of connexins typical of an actively renewing human interfollicular epidermis, and (ii) provide a model that reproduces the tridimensional organization of intact epidermis and that is amenable for experimentally testing the function of junctional communication between human keratinocytes. More... »

PAGES

278-285

References to SciGraph publications

  • 1998-11. Functional defects of Cx26 resulting from a heterozygous missense mutation in a family with dominant deaf-mutism and palmoplantar keratoderma in HUMAN GENETICS
  • 1997-05. Connexin 26 mutations in hereditary non-syndromic sensorineural deafness in NATURE
  • 1992-10. Gap-Junctional Protein Connexin 43 Is Expressed in Dermis and Epidermis of Human Skin: Differential Modulation by Retinoids in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1996-07. Successful Treatment of Chronic Leg Ulcers with Epidermal Equivalents Generated from Cultured Autologous Outer Root Sheath Cells in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1998-12. Mutations in the human connexin gene GJB3 cause erythrokeratodermia variabilis in NATURE GENETICS
  • 1999-11. Selective transfer of endogenous metabolites through gap junctions composed of different connexins in NATURE CELL BIOLOGY
  • 1998-07. Upregulation of Connexin 26 Between Keratinocytes of Psoriatic Lesions in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1999-06. Human melanocytes grown in epidermal equivalents transfer their melanin to follicular outer root sheath keratinocytes in ARCHIVES OF DERMATOLOGICAL RESEARCH
  • 1999-03. Upregulation of Connexin 26 is a Feature of Keratinocyte Differentiation in Hyperproliferative Epidermis, Vaginal Epithelium, and Buccal Epithelium in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1994-08. Topography of Mammalian Connexins in Human Skin in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1996-10. Upregulation of gap junction protein connexin43 in alveolar epithelial cells of rats with radiation-induced pulmonary fibrosis in HISTOCHEMISTRY AND CELL BIOLOGY
  • 1997-02. Identification of Aberrantly Regulated Genes in Diseased Skin Using the cDNA Differential Display Technique in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1046/j.1523-1747.2000.00043.x

    DOI

    http://dx.doi.org/10.1046/j.1523-1747.2000.00043.x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024926176

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10951247


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