Differential Expression of Connexins During Stratification of Human Keratinocytes View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2000-08

AUTHORS

Ludovic Wiszniewski, Alain Limat, Jean-Hilaire Saurat, Paolo Meda, Denis Salomon

ABSTRACT

To assess whether gap junctions and connexins change during keratinocyte differentiation, we have studied epidermal equivalents obtained in organotypic cultures of keratinocytes from the outer root sheath of human hair follicles. These reconstituted tissues exhibit a number of differentiation and proliferation markers of human epidermis, including gap junctions, connexins, and K6 and Ki67 proteins. Immunostaining and northern blots showed that gap junctions of the epidermal equivalents were made of Cx26 and Cx43. Cx26 was expressed in all keratinocyte layers, throughout the development of the epidermal equivalents. In contrast, Cx43 was initially observed only in the basal layer of keratinocytes and became detectable in the stratum spinosum and granulosum only after the epidermal equivalents had thickened. The levels of Cx26 and its transcript markedly increased as a function of stratification of the epidermal equivalents, whereas those of Cx43 remained almost constant. Microinjection of Lucifer Yellow into individual keratinocytes showed that gap junctions were similarly permeable at all stages of development of the epidermal equivalents. The data show that epidermal equivalents (i) feature a pattern of connexins typical of an actively renewing human interfollicular epidermis, and (ii) provide a model that reproduces the tridimensional organization of intact epidermis and that is amenable for experimentally testing the function of junctional communication between human keratinocytes. More... »

PAGES

278-285

References to SciGraph publications

  • 1998-11. Functional defects of Cx26 resulting from a heterozygous missense mutation in a family with dominant deaf-mutism and palmoplantar keratoderma in HUMAN GENETICS
  • 1997-05. Connexin 26 mutations in hereditary non-syndromic sensorineural deafness in NATURE
  • 1992-10. Gap-Junctional Protein Connexin 43 Is Expressed in Dermis and Epidermis of Human Skin: Differential Modulation by Retinoids in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1996-07. Successful Treatment of Chronic Leg Ulcers with Epidermal Equivalents Generated from Cultured Autologous Outer Root Sheath Cells in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1998-12. Mutations in the human connexin gene GJB3 cause erythrokeratodermia variabilis in NATURE GENETICS
  • 1999-11. Selective transfer of endogenous metabolites through gap junctions composed of different connexins in NATURE CELL BIOLOGY
  • 1998-07. Upregulation of Connexin 26 Between Keratinocytes of Psoriatic Lesions in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1999-06. Human melanocytes grown in epidermal equivalents transfer their melanin to follicular outer root sheath keratinocytes in ARCHIVES OF DERMATOLOGICAL RESEARCH
  • 1999-03. Upregulation of Connexin 26 is a Feature of Keratinocyte Differentiation in Hyperproliferative Epidermis, Vaginal Epithelium, and Buccal Epithelium in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1994-08. Topography of Mammalian Connexins in Human Skin in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1996-10. Upregulation of gap junction protein connexin43 in alveolar epithelial cells of rats with radiation-induced pulmonary fibrosis in HISTOCHEMISTRY AND CELL BIOLOGY
  • 1997-02. Identification of Aberrantly Regulated Genes in Diseased Skin Using the cDNA Differential Display Technique in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1046/j.1523-1747.2000.00043.x

    DOI

    http://dx.doi.org/10.1046/j.1523-1747.2000.00043.x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024926176

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10951247


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1115", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Pharmacology and Pharmaceutical Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Differentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Division", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Cultured", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Connexin 26", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Connexin 43", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Connexins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Epidermis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gap Junctions", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hair Follicle", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Ion Channels", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Keratinocytes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Permeability", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Geneva", 
              "id": "https://www.grid.ac/institutes/grid.8591.5", 
              "name": [
                "Department of Dermatology (DHURDV), Switzerland", 
                "Department of Morphology, University of Geneva, Switzerland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wiszniewski", 
            "givenName": "Ludovic", 
            "id": "sg:person.01021461644.18", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021461644.18"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Department of Dermatology (DHURDV), Switzerland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Limat", 
            "givenName": "Alain", 
            "id": "sg:person.01342517334.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342517334.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Department of Dermatology (DHURDV), Switzerland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Saurat", 
            "givenName": "Jean-Hilaire", 
            "id": "sg:person.01134015727.07", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01134015727.07"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Meda", 
            "givenName": "Paolo", 
            "id": "sg:person.01045054306.23", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01045054306.23"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Department of Dermatology (DHURDV), Switzerland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Salomon", 
            "givenName": "Denis", 
            "id": "sg:person.01200741554.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200741554.04"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1111/j.1365-2133.1986.tb02778.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000603136"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1098-2744(199610)17:2<49::aid-mc1>3.0.co;2-o", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001338049"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/b978-0-444-89871-5.50037-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001785751"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s004030050417", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002721831", 
              "https://doi.org/10.1007/s004030050417"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.91.14.6453", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007876030"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1097-0177(199712)210:4<417::aid-aja6>3.0.co;2-j", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008665641"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.111.2.589", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009237125"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1091/mbc.6.11.1491", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009900194"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/387080a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017615074", 
              "https://doi.org/10.1038/387080a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.103.5.1945", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019775501"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/aja.1002000102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019927520"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/15693", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022453752", 
              "https://doi.org/10.1038/15693"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/15693", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022453752", 
              "https://doi.org/10.1038/15693"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1046/j.1523-1747.1999.00512.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022576938", 
              "https://doi.org/10.1046/j.1523-1747.1999.00512.x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1172/jci113578", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023715213"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1111/1523-1747.ep12616154", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024984077", 
              "https://doi.org/10.1111/1523-1747.ep12616154"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s004390050839", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028237051", 
              "https://doi.org/10.1007/s004390050839"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1111/1523-1747.ep12334217", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028270677", 
              "https://doi.org/10.1111/1523-1747.ep12334217"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.118.5.1213", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030840766"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.109.6.3027", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037466582"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1111/1523-1747.ep12393218", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038363245", 
              "https://doi.org/10.1111/1523-1747.ep12393218"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/hmg/8.7.1237", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041262595"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1046/j.1523-1747.1998.00248.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042019756", 
              "https://doi.org/10.1046/j.1523-1747.1998.00248.x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/ijc.2910510404", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043570447"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1111/1523-1747.ep12298415", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044507255", 
              "https://doi.org/10.1111/1523-1747.ep12298415"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0092-8674(00)81282-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047804901"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/3840", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048391612", 
              "https://doi.org/10.1038/3840"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/3840", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048391612", 
              "https://doi.org/10.1038/3840"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/hmg/6.9.1605", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048855394"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/002215549704500904", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050815111"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/002215549704500904", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050815111"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02473301", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053275802", 
              "https://doi.org/10.1007/bf02473301"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02473301", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053275802", 
              "https://doi.org/10.1007/bf02473301"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1136/jmg.37.1.50", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053413422"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1210/endo.133.5.8404689", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1064278826"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075324278", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077003214", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079542171", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083254212", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2000-08", 
        "datePublishedReg": "2000-08-01", 
        "description": "To assess whether gap junctions and connexins change during keratinocyte differentiation, we have studied epidermal equivalents obtained in organotypic cultures of keratinocytes from the outer root sheath of human hair follicles. These reconstituted tissues exhibit a number of differentiation and proliferation markers of human epidermis, including gap junctions, connexins, and K6 and Ki67 proteins. Immunostaining and northern blots showed that gap junctions of the epidermal equivalents were made of Cx26 and Cx43. Cx26 was expressed in all keratinocyte layers, throughout the development of the epidermal equivalents. In contrast, Cx43 was initially observed only in the basal layer of keratinocytes and became detectable in the stratum spinosum and granulosum only after the epidermal equivalents had thickened. The levels of Cx26 and its transcript markedly increased as a function of stratification of the epidermal equivalents, whereas those of Cx43 remained almost constant. Microinjection of Lucifer Yellow into individual keratinocytes showed that gap junctions were similarly permeable at all stages of development of the epidermal equivalents. The data show that epidermal equivalents (i) feature a pattern of connexins typical of an actively renewing human interfollicular epidermis, and (ii) provide a model that reproduces the tridimensional organization of intact epidermis and that is amenable for experimentally testing the function of junctional communication between human keratinocytes.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1046/j.1523-1747.2000.00043.x", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1020153", 
            "issn": [
              "0022-202X", 
              "1523-1747"
            ], 
            "name": "Journal of Investigative Dermatology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "2", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "115"
          }
        ], 
        "name": "Differential Expression of Connexins During Stratification of Human Keratinocytes", 
        "pagination": "278-285", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "76bf98d9435983badcc6daf34c964b71c5d0b064056481e81fc4e190b234d84d"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "10951247"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0426720"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1046/j.1523-1747.2000.00043.x"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1024926176"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1046/j.1523-1747.2000.00043.x", 
          "https://app.dimensions.ai/details/publication/pub.1024926176"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T16:29", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000424.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/jid/journal/v115/n2/full/5600784a.html"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1046/j.1523-1747.2000.00043.x'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1046/j.1523-1747.2000.00043.x'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1046/j.1523-1747.2000.00043.x'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1046/j.1523-1747.2000.00043.x'


     

    This table displays all metadata directly associated to this object as RDF triples.

    268 TRIPLES      21 PREDICATES      77 URIs      34 LITERALS      22 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1046/j.1523-1747.2000.00043.x schema:about N01e34fec7b2c4e27bacec4b72356418d
    2 N1b5b8960bccf4953a923b16cf6c6737d
    3 N34bb5523ffba4f0aa631d02faf1fc12c
    4 N5217e28ec6eb44f9acda9bf8d1389083
    5 N557e3f87ba014bbeb3154766f98b7e8e
    6 N66c8dbb606a34fed9d178e39acf50995
    7 N827868ffd46343fa93b6a97804bc1c00
    8 Na30f7ccba2594066bad6f97531643148
    9 Ncd1a1d53e6f44569a06b6fd4675f0186
    10 Nd147e45e3c514ba29745c278917f4689
    11 Ne4e7b469dce44701a8fc0a18afc6100a
    12 Nefe493dd239b41d7bd1fcfd972bd24a6
    13 Nf6adb59bba764208826d4988e6e5bade
    14 anzsrc-for:11
    15 anzsrc-for:1115
    16 schema:author Nbba53243f05f4ad7b528ce48fb2d48c3
    17 schema:citation sg:pub.10.1007/bf02473301
    18 sg:pub.10.1007/s004030050417
    19 sg:pub.10.1007/s004390050839
    20 sg:pub.10.1038/15693
    21 sg:pub.10.1038/3840
    22 sg:pub.10.1038/387080a0
    23 sg:pub.10.1046/j.1523-1747.1998.00248.x
    24 sg:pub.10.1046/j.1523-1747.1999.00512.x
    25 sg:pub.10.1111/1523-1747.ep12298415
    26 sg:pub.10.1111/1523-1747.ep12334217
    27 sg:pub.10.1111/1523-1747.ep12393218
    28 sg:pub.10.1111/1523-1747.ep12616154
    29 https://app.dimensions.ai/details/publication/pub.1075324278
    30 https://app.dimensions.ai/details/publication/pub.1077003214
    31 https://app.dimensions.ai/details/publication/pub.1079542171
    32 https://app.dimensions.ai/details/publication/pub.1083254212
    33 https://doi.org/10.1002/(sici)1097-0177(199712)210:4<417::aid-aja6>3.0.co;2-j
    34 https://doi.org/10.1002/(sici)1098-2744(199610)17:2<49::aid-mc1>3.0.co;2-o
    35 https://doi.org/10.1002/aja.1002000102
    36 https://doi.org/10.1002/ijc.2910510404
    37 https://doi.org/10.1016/b978-0-444-89871-5.50037-7
    38 https://doi.org/10.1016/s0092-8674(00)81282-9
    39 https://doi.org/10.1073/pnas.91.14.6453
    40 https://doi.org/10.1083/jcb.103.5.1945
    41 https://doi.org/10.1083/jcb.109.6.3027
    42 https://doi.org/10.1083/jcb.111.2.589
    43 https://doi.org/10.1083/jcb.118.5.1213
    44 https://doi.org/10.1091/mbc.6.11.1491
    45 https://doi.org/10.1093/hmg/6.9.1605
    46 https://doi.org/10.1093/hmg/8.7.1237
    47 https://doi.org/10.1111/j.1365-2133.1986.tb02778.x
    48 https://doi.org/10.1136/jmg.37.1.50
    49 https://doi.org/10.1172/jci113578
    50 https://doi.org/10.1177/002215549704500904
    51 https://doi.org/10.1210/endo.133.5.8404689
    52 schema:datePublished 2000-08
    53 schema:datePublishedReg 2000-08-01
    54 schema:description To assess whether gap junctions and connexins change during keratinocyte differentiation, we have studied epidermal equivalents obtained in organotypic cultures of keratinocytes from the outer root sheath of human hair follicles. These reconstituted tissues exhibit a number of differentiation and proliferation markers of human epidermis, including gap junctions, connexins, and K6 and Ki67 proteins. Immunostaining and northern blots showed that gap junctions of the epidermal equivalents were made of Cx26 and Cx43. Cx26 was expressed in all keratinocyte layers, throughout the development of the epidermal equivalents. In contrast, Cx43 was initially observed only in the basal layer of keratinocytes and became detectable in the stratum spinosum and granulosum only after the epidermal equivalents had thickened. The levels of Cx26 and its transcript markedly increased as a function of stratification of the epidermal equivalents, whereas those of Cx43 remained almost constant. Microinjection of Lucifer Yellow into individual keratinocytes showed that gap junctions were similarly permeable at all stages of development of the epidermal equivalents. The data show that epidermal equivalents (i) feature a pattern of connexins typical of an actively renewing human interfollicular epidermis, and (ii) provide a model that reproduces the tridimensional organization of intact epidermis and that is amenable for experimentally testing the function of junctional communication between human keratinocytes.
    55 schema:genre research_article
    56 schema:inLanguage en
    57 schema:isAccessibleForFree true
    58 schema:isPartOf N3daae7b121e64a67973107bceae7b8bd
    59 Na64bd7ea777f4c108439f3fe71afe317
    60 sg:journal.1020153
    61 schema:name Differential Expression of Connexins During Stratification of Human Keratinocytes
    62 schema:pagination 278-285
    63 schema:productId N5da5b71427db4564a13acfe09de772ca
    64 N7239209369c94087912a52234c392eee
    65 N8e22154bff144264a6f4e74b161c20ac
    66 Nae1cd91466da4a73bd9588d3cf45d5a6
    67 Nd3fd6390fb75487e97e8933f1c81671f
    68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024926176
    69 https://doi.org/10.1046/j.1523-1747.2000.00043.x
    70 schema:sdDatePublished 2019-04-10T16:29
    71 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    72 schema:sdPublisher N6a76b32da1ae462989b8e6ab11ce6a65
    73 schema:url http://www.nature.com/jid/journal/v115/n2/full/5600784a.html
    74 sgo:license sg:explorer/license/
    75 sgo:sdDataset articles
    76 rdf:type schema:ScholarlyArticle
    77 N01e34fec7b2c4e27bacec4b72356418d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    78 schema:name Humans
    79 rdf:type schema:DefinedTerm
    80 N1b5b8960bccf4953a923b16cf6c6737d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    81 schema:name Ion Channels
    82 rdf:type schema:DefinedTerm
    83 N34bb5523ffba4f0aa631d02faf1fc12c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    84 schema:name Keratinocytes
    85 rdf:type schema:DefinedTerm
    86 N3daae7b121e64a67973107bceae7b8bd schema:issueNumber 2
    87 rdf:type schema:PublicationIssue
    88 N4b1dfde3597a45878d1ab96520e1fdc8 rdf:first sg:person.01134015727.07
    89 rdf:rest N517d0567f85d4693bec93c1b7ce02421
    90 N517d0567f85d4693bec93c1b7ce02421 rdf:first sg:person.01045054306.23
    91 rdf:rest N755c66ce5fd745a7b003da054357e0db
    92 N5217e28ec6eb44f9acda9bf8d1389083 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Gap Junctions
    94 rdf:type schema:DefinedTerm
    95 N557e3f87ba014bbeb3154766f98b7e8e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Connexin 43
    97 rdf:type schema:DefinedTerm
    98 N5b6bfe05b43046a085a11ea6887c6ce6 schema:name Department of Dermatology (DHURDV), Switzerland
    99 rdf:type schema:Organization
    100 N5da5b71427db4564a13acfe09de772ca schema:name doi
    101 schema:value 10.1046/j.1523-1747.2000.00043.x
    102 rdf:type schema:PropertyValue
    103 N66c8dbb606a34fed9d178e39acf50995 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name Connexins
    105 rdf:type schema:DefinedTerm
    106 N6a76b32da1ae462989b8e6ab11ce6a65 schema:name Springer Nature - SN SciGraph project
    107 rdf:type schema:Organization
    108 N6d43576adf1a4c1dbb566185e084c9c6 schema:name Department of Dermatology (DHURDV), Switzerland
    109 rdf:type schema:Organization
    110 N7239209369c94087912a52234c392eee schema:name pubmed_id
    111 schema:value 10951247
    112 rdf:type schema:PropertyValue
    113 N755c66ce5fd745a7b003da054357e0db rdf:first sg:person.01200741554.04
    114 rdf:rest rdf:nil
    115 N827868ffd46343fa93b6a97804bc1c00 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Cell Division
    117 rdf:type schema:DefinedTerm
    118 N8e22154bff144264a6f4e74b161c20ac schema:name dimensions_id
    119 schema:value pub.1024926176
    120 rdf:type schema:PropertyValue
    121 Na30f7ccba2594066bad6f97531643148 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Permeability
    123 rdf:type schema:DefinedTerm
    124 Na64bd7ea777f4c108439f3fe71afe317 schema:volumeNumber 115
    125 rdf:type schema:PublicationVolume
    126 Na9e358beec654b039f1b2d2963270ef3 schema:name Department of Dermatology (DHURDV), Switzerland
    127 rdf:type schema:Organization
    128 Nae1cd91466da4a73bd9588d3cf45d5a6 schema:name nlm_unique_id
    129 schema:value 0426720
    130 rdf:type schema:PropertyValue
    131 Nbba53243f05f4ad7b528ce48fb2d48c3 rdf:first sg:person.01021461644.18
    132 rdf:rest Ncbbcbf21fc1e43779d6b215d8648d43e
    133 Ncbbcbf21fc1e43779d6b215d8648d43e rdf:first sg:person.01342517334.20
    134 rdf:rest N4b1dfde3597a45878d1ab96520e1fdc8
    135 Ncd1a1d53e6f44569a06b6fd4675f0186 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    136 schema:name Hair Follicle
    137 rdf:type schema:DefinedTerm
    138 Nd147e45e3c514ba29745c278917f4689 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    139 schema:name Cells, Cultured
    140 rdf:type schema:DefinedTerm
    141 Nd3fd6390fb75487e97e8933f1c81671f schema:name readcube_id
    142 schema:value 76bf98d9435983badcc6daf34c964b71c5d0b064056481e81fc4e190b234d84d
    143 rdf:type schema:PropertyValue
    144 Ne4e7b469dce44701a8fc0a18afc6100a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    145 schema:name Connexin 26
    146 rdf:type schema:DefinedTerm
    147 Nefe493dd239b41d7bd1fcfd972bd24a6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    148 schema:name Cell Differentiation
    149 rdf:type schema:DefinedTerm
    150 Nf6adb59bba764208826d4988e6e5bade schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    151 schema:name Epidermis
    152 rdf:type schema:DefinedTerm
    153 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    154 schema:name Medical and Health Sciences
    155 rdf:type schema:DefinedTerm
    156 anzsrc-for:1115 schema:inDefinedTermSet anzsrc-for:
    157 schema:name Pharmacology and Pharmaceutical Sciences
    158 rdf:type schema:DefinedTerm
    159 sg:journal.1020153 schema:issn 0022-202X
    160 1523-1747
    161 schema:name Journal of Investigative Dermatology
    162 rdf:type schema:Periodical
    163 sg:person.01021461644.18 schema:affiliation https://www.grid.ac/institutes/grid.8591.5
    164 schema:familyName Wiszniewski
    165 schema:givenName Ludovic
    166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021461644.18
    167 rdf:type schema:Person
    168 sg:person.01045054306.23 schema:familyName Meda
    169 schema:givenName Paolo
    170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01045054306.23
    171 rdf:type schema:Person
    172 sg:person.01134015727.07 schema:affiliation N6d43576adf1a4c1dbb566185e084c9c6
    173 schema:familyName Saurat
    174 schema:givenName Jean-Hilaire
    175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01134015727.07
    176 rdf:type schema:Person
    177 sg:person.01200741554.04 schema:affiliation Na9e358beec654b039f1b2d2963270ef3
    178 schema:familyName Salomon
    179 schema:givenName Denis
    180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200741554.04
    181 rdf:type schema:Person
    182 sg:person.01342517334.20 schema:affiliation N5b6bfe05b43046a085a11ea6887c6ce6
    183 schema:familyName Limat
    184 schema:givenName Alain
    185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342517334.20
    186 rdf:type schema:Person
    187 sg:pub.10.1007/bf02473301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053275802
    188 https://doi.org/10.1007/bf02473301
    189 rdf:type schema:CreativeWork
    190 sg:pub.10.1007/s004030050417 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002721831
    191 https://doi.org/10.1007/s004030050417
    192 rdf:type schema:CreativeWork
    193 sg:pub.10.1007/s004390050839 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028237051
    194 https://doi.org/10.1007/s004390050839
    195 rdf:type schema:CreativeWork
    196 sg:pub.10.1038/15693 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022453752
    197 https://doi.org/10.1038/15693
    198 rdf:type schema:CreativeWork
    199 sg:pub.10.1038/3840 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048391612
    200 https://doi.org/10.1038/3840
    201 rdf:type schema:CreativeWork
    202 sg:pub.10.1038/387080a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017615074
    203 https://doi.org/10.1038/387080a0
    204 rdf:type schema:CreativeWork
    205 sg:pub.10.1046/j.1523-1747.1998.00248.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1042019756
    206 https://doi.org/10.1046/j.1523-1747.1998.00248.x
    207 rdf:type schema:CreativeWork
    208 sg:pub.10.1046/j.1523-1747.1999.00512.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022576938
    209 https://doi.org/10.1046/j.1523-1747.1999.00512.x
    210 rdf:type schema:CreativeWork
    211 sg:pub.10.1111/1523-1747.ep12298415 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044507255
    212 https://doi.org/10.1111/1523-1747.ep12298415
    213 rdf:type schema:CreativeWork
    214 sg:pub.10.1111/1523-1747.ep12334217 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028270677
    215 https://doi.org/10.1111/1523-1747.ep12334217
    216 rdf:type schema:CreativeWork
    217 sg:pub.10.1111/1523-1747.ep12393218 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038363245
    218 https://doi.org/10.1111/1523-1747.ep12393218
    219 rdf:type schema:CreativeWork
    220 sg:pub.10.1111/1523-1747.ep12616154 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024984077
    221 https://doi.org/10.1111/1523-1747.ep12616154
    222 rdf:type schema:CreativeWork
    223 https://app.dimensions.ai/details/publication/pub.1075324278 schema:CreativeWork
    224 https://app.dimensions.ai/details/publication/pub.1077003214 schema:CreativeWork
    225 https://app.dimensions.ai/details/publication/pub.1079542171 schema:CreativeWork
    226 https://app.dimensions.ai/details/publication/pub.1083254212 schema:CreativeWork
    227 https://doi.org/10.1002/(sici)1097-0177(199712)210:4<417::aid-aja6>3.0.co;2-j schema:sameAs https://app.dimensions.ai/details/publication/pub.1008665641
    228 rdf:type schema:CreativeWork
    229 https://doi.org/10.1002/(sici)1098-2744(199610)17:2<49::aid-mc1>3.0.co;2-o schema:sameAs https://app.dimensions.ai/details/publication/pub.1001338049
    230 rdf:type schema:CreativeWork
    231 https://doi.org/10.1002/aja.1002000102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019927520
    232 rdf:type schema:CreativeWork
    233 https://doi.org/10.1002/ijc.2910510404 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043570447
    234 rdf:type schema:CreativeWork
    235 https://doi.org/10.1016/b978-0-444-89871-5.50037-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001785751
    236 rdf:type schema:CreativeWork
    237 https://doi.org/10.1016/s0092-8674(00)81282-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047804901
    238 rdf:type schema:CreativeWork
    239 https://doi.org/10.1073/pnas.91.14.6453 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007876030
    240 rdf:type schema:CreativeWork
    241 https://doi.org/10.1083/jcb.103.5.1945 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019775501
    242 rdf:type schema:CreativeWork
    243 https://doi.org/10.1083/jcb.109.6.3027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037466582
    244 rdf:type schema:CreativeWork
    245 https://doi.org/10.1083/jcb.111.2.589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009237125
    246 rdf:type schema:CreativeWork
    247 https://doi.org/10.1083/jcb.118.5.1213 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030840766
    248 rdf:type schema:CreativeWork
    249 https://doi.org/10.1091/mbc.6.11.1491 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009900194
    250 rdf:type schema:CreativeWork
    251 https://doi.org/10.1093/hmg/6.9.1605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048855394
    252 rdf:type schema:CreativeWork
    253 https://doi.org/10.1093/hmg/8.7.1237 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041262595
    254 rdf:type schema:CreativeWork
    255 https://doi.org/10.1111/j.1365-2133.1986.tb02778.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1000603136
    256 rdf:type schema:CreativeWork
    257 https://doi.org/10.1136/jmg.37.1.50 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053413422
    258 rdf:type schema:CreativeWork
    259 https://doi.org/10.1172/jci113578 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023715213
    260 rdf:type schema:CreativeWork
    261 https://doi.org/10.1177/002215549704500904 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050815111
    262 rdf:type schema:CreativeWork
    263 https://doi.org/10.1210/endo.133.5.8404689 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064278826
    264 rdf:type schema:CreativeWork
    265 https://www.grid.ac/institutes/grid.8591.5 schema:alternateName University of Geneva
    266 schema:name Department of Dermatology (DHURDV), Switzerland
    267 Department of Morphology, University of Geneva, Switzerland
    268 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...