Bullous Pemphigoid Sera that Contain Antibodies to BPAg2 also Contain Antibodies to LABD97 that Recognize Epitopes Distal to the NC16A ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1999-02

AUTHORS

C A Egan, T B Taylor, L J Meyer, M J Petersen, J J Zone

ABSTRACT

IgG antibodies from the sera of some patients with bullous pemphigoid (BP) react with a 180 kDa protein termed BPAg2. Antibodies in BP are directed to an extracellular noncollagenous domain of this protein termed NC16A. Our group has recently shown that a portion of the extracellular domain of BPAg2 is identical to LABD97 on the basis of amino acid sequencing. We evaluated sera from 33 patients with BP with circulating IgG antibodies on indirect immunofluorescence, which stained the epidermal side of split skin with titers ranging from 1:40 to 1:640. Immunoblotting was performed against (i) two preparations of proteins from epidermal extract, one containing BPAg2 and one containing LABD97, and (ii) the recombinant NC16A domain of the BPAg2 protein. Twelve sera reacted with the BPAg2 protein. Ten of these also reacted strongly with the NC16A domain. Nine of the 12 sera also reacted with the LABD97 antigen. Bound antibodies were eluted from the 97 kDa band and reapplied to split skin where they bound to the epidermal side. The eluted antibodies also reacted to the BPAg2 protein from the epidermal extract, but did not react with the NC16A domain on immunoblot. We conclude that these nine sera react with an epitope present within BPAg2 and LABD97 but not within the NC16A domain. This epitope is therefore distal to the previously described epitopes in BP. In BP, epitope spreading may occur and antibodies may be produced that recognize the distal portion of the BPAg2 molecule identical to LABD97 but that do not involve the NC16A domain. More... »

PAGES

148-152

References to SciGraph publications

  • 1998-03. The 97 kDa Linear IgA Bullous Disease Antigen Is Identical to a Portion of the Extracellular Domain of the 180 kDa Bullous Pemphigoid Antigen, BPAg2 in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1990-06. Bullous Pemphigoid Antigens: Extraction and Degradation of Antigens During Epidermal Preparation in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1997-04. Bullous Pemphigoid and Linear IgA Dermatosis Sera Recognize a Similar 120-kDa Keratinocyte Collagenous Glycoprotein with Antigenic Cross-Reactivity to BP180 in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1989-01. Diagnosis of Adult Linear IgA Dermatosis by Immunoelectronmicroscopy in 16 Patients with Linear IgA Deposits in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1977-12. CIRCULATING IgA ANTI-BASEMENT MEMBRANE ZONE ANTIBODIES IN DERMATITIS HERPETIFORMIS in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1997-09. LAD-1 Is Absent in a Subset of Junctional Epidermolysis Bullosa Patients in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1992-09. Cloning and Primary Structural Analysis of the Bullous Pemphigoid Autoantigen BP180 in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1997-10. Tight Clustering of Extracellular BP180 Epitopes Recognized by Bullous Pemphigoid Autoantibodies in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1976-10. ULTRASTRUCTURAL LOCALIZATION OF IMMUNOGLOBULINS IN SKIN OF PATIENTS WITH DERMATITIS HERPETIFORMIS in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • 1970-08. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 in NATURE
  • 1998-02. Epitope Spreading: Lessons From Autoimmune Skin Diseases in JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1046/j.1523-1747.1999.00490.x

    DOI

    http://dx.doi.org/10.1046/j.1523-1747.1999.00490.x

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9989788


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