Identification of methicillin-resistant Staphylococcus aureus-specific peptides for targeted photoantimicrobial chemotherapy View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-04

AUTHORS

Rebecca V. Vince, Leigh A. Madden, Cristina M. A. Alonso, Huguette Savoie, Ross W. Boyle, Martin Todman, Tim Paget, John Greenman

ABSTRACT

The increasing prevalence of multi-drug resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), necessitates development of alternative modes of bacterial targeting which are not hindered by antibiotic resistance and minimise collateral damage. To achieve this, the FliTrxTM bacterially-displayed peptide library was panned against MRSA and randomly selected clones (n = 20) were DNA sequenced. One selected peptide was synthesised as both cyclic and linear constructs. Binding of the cyclic construct was observed by flow cytometry against isolates of MRSA whilst the linear construct showed low affinity. Low reactivity was observed with other Staphylococcal sp., Gram-negative bacteria and human keratinocytes. The selected peptide was also cloned in-frame, within the thioredoxin gene into the pPROTet.E 6xHN vector for protein expression. A porphyrin photosensitiser (5-(4-isothiocyanatophenyl)-10,15,20-tris(4-N-methylpyridiniumyl)porphyrin trichloride) was conjugated to the recombinant protein and the in vitro cytotoxic effect of the resulting bioconjugate was determined against MRSA and other non-specific bacterial and mammalian cell lines. Photoantimicrobial chemotherapy (PACT) using the bioconjugate showed a 66% reduction in MRSA growth in comparison with non-irradiated cells. This work demonstrates the potential to isolate peptides with binding specificity against MRSA that can be used for targeted PACT, providing an effective alternative to antibody targeting. More... »

PAGES

515-522

Identifiers

URI

http://scigraph.springernature.com/pub.10.1039/c0pp00267d

DOI

http://dx.doi.org/10.1039/c0pp00267d

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050163459

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21180705


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Anti-Bacterial Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Keratinocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Methicillin-Resistant Staphylococcus aureus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Photosensitizing Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Porphyrins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thioredoxins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Postgraduate Medical Institute, The University of Hull, Room 512, Wolfson building, Cottingham Road, HU6 7RX, Hull, UK", 
          "id": "http://www.grid.ac/institutes/grid.9481.4", 
          "name": [
            "Postgraduate Medical Institute, The University of Hull, Room 512, Wolfson building, Cottingham Road, HU6 7RX, Hull, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vince", 
        "givenName": "Rebecca V.", 
        "id": "sg:person.01170622731.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170622731.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Postgraduate Medical Institute, The University of Hull, Room 512, Wolfson building, Cottingham Road, HU6 7RX, Hull, UK", 
          "id": "http://www.grid.ac/institutes/grid.9481.4", 
          "name": [
            "Postgraduate Medical Institute, The University of Hull, Room 512, Wolfson building, Cottingham Road, HU6 7RX, Hull, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Madden", 
        "givenName": "Leigh A.", 
        "id": "sg:person.01006261131.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01006261131.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Chemistry, The University of Hull, HU6 7RX, UK", 
          "id": "http://www.grid.ac/institutes/grid.9481.4", 
          "name": [
            "Department of Chemistry, The University of Hull, HU6 7RX, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Alonso", 
        "givenName": "Cristina M. A.", 
        "id": "sg:person.0663342455.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663342455.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Chemistry, The University of Hull, HU6 7RX, UK", 
          "id": "http://www.grid.ac/institutes/grid.9481.4", 
          "name": [
            "Department of Chemistry, The University of Hull, HU6 7RX, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Savoie", 
        "givenName": "Huguette", 
        "id": "sg:person.01154124213.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154124213.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Chemistry, The University of Hull, HU6 7RX, UK", 
          "id": "http://www.grid.ac/institutes/grid.9481.4", 
          "name": [
            "Department of Chemistry, The University of Hull, HU6 7RX, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Boyle", 
        "givenName": "Ross W.", 
        "id": "sg:person.01270352613.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270352613.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Smith & Nephew Research Centre, YO10 5DF, York, UK", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Smith & Nephew Research Centre, YO10 5DF, York, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Todman", 
        "givenName": "Martin", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Medway School of Pharmacy, The University of Kent, ME4 4TB, UK", 
          "id": "http://www.grid.ac/institutes/grid.9759.2", 
          "name": [
            "Medway School of Pharmacy, The University of Kent, ME4 4TB, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Paget", 
        "givenName": "Tim", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Postgraduate Medical Institute, The University of Hull, Room 512, Wolfson building, Cottingham Road, HU6 7RX, Hull, UK", 
          "id": "http://www.grid.ac/institutes/grid.9481.4", 
          "name": [
            "Postgraduate Medical Institute, The University of Hull, Room 512, Wolfson building, Cottingham Road, HU6 7RX, Hull, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Greenman", 
        "givenName": "John", 
        "id": "sg:person.0754170773.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754170773.23"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1186/1471-2180-9-27", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045409023", 
          "https://doi.org/10.1186/1471-2180-9-27"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s101030050021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033947620", 
          "https://doi.org/10.1007/s101030050021"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10103-006-0409-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044366929", 
          "https://doi.org/10.1007/s10103-006-0409-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0794-703", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040927659", 
          "https://doi.org/10.1038/nbt0794-703"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bjc.6602517", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016860593", 
          "https://doi.org/10.1038/sj.bjc.6602517"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10434-000-0743-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006319679", 
          "https://doi.org/10.1007/s10434-000-0743-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0495-366", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031929455", 
          "https://doi.org/10.1038/nbt0495-366"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1039/b311900a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035001576", 
          "https://doi.org/10.1039/b311900a"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-04", 
    "datePublishedReg": "2011-04-01", 
    "description": "The increasing prevalence of multi-drug resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), necessitates development of alternative modes of bacterial targeting which are not hindered by antibiotic resistance and minimise collateral damage. To achieve this, the FliTrxTM bacterially-displayed peptide library was panned against MRSA and randomly selected clones (n = 20) were DNA sequenced. One selected peptide was synthesised as both cyclic and linear constructs. Binding of the cyclic construct was observed by flow cytometry against isolates of MRSA whilst the linear construct showed low affinity. Low reactivity was observed with other Staphylococcal sp., Gram-negative bacteria and human keratinocytes. The selected peptide was also cloned in-frame, within the thioredoxin gene into the pPROTet.E 6xHN vector for protein expression. A porphyrin photosensitiser (5-(4-isothiocyanatophenyl)-10,15,20-tris(4-N-methylpyridiniumyl)porphyrin trichloride) was conjugated to the recombinant protein and the in vitro cytotoxic effect of the resulting bioconjugate was determined against MRSA and other non-specific bacterial and mammalian cell lines. Photoantimicrobial chemotherapy (PACT) using the bioconjugate showed a 66% reduction in MRSA growth in comparison with non-irradiated cells. This work demonstrates the potential to isolate peptides with binding specificity against MRSA that can be used for targeted PACT, providing an effective alternative to antibody targeting.", 
    "genre": "article", 
    "id": "sg:pub.10.1039/c0pp00267d", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1029729", 
        "issn": [
          "1474-905X", 
          "1474-9092"
        ], 
        "name": "Photochemical & Photobiological Sciences", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "keywords": [
      "methicillin-resistant Staphylococcus aureus", 
      "isolates of MRSA", 
      "minimise collateral damage", 
      "multi-drug resistant bacteria", 
      "photoantimicrobial chemotherapy", 
      "MRSA growth", 
      "flow cytometry", 
      "non-irradiated cells", 
      "Staphylococcal sp", 
      "protein expression", 
      "bacterial targeting", 
      "antibiotic resistance", 
      "cytotoxic effects", 
      "human keratinocytes", 
      "chemotherapy", 
      "Staphylococcus aureus", 
      "cell lines", 
      "effective alternative", 
      "resistant bacteria", 
      "porphyrin photosensitiser", 
      "low affinity", 
      "peptides", 
      "collateral damage", 
      "prevalence", 
      "Gram-negative bacteria", 
      "antibodies", 
      "cytometry", 
      "keratinocytes", 
      "mammalian cell lines", 
      "aureus", 
      "peptide libraries", 
      "cells", 
      "isolates", 
      "expression", 
      "recombinant proteins", 
      "targeting", 
      "damage", 
      "specificity", 
      "bacteria", 
      "alternative modes", 
      "cyclic constructs", 
      "protein", 
      "clones", 
      "genes", 
      "reduction", 
      "resistance", 
      "reactivity", 
      "photosensitiser", 
      "effect", 
      "constructs", 
      "affinity", 
      "development", 
      "DNA", 
      "identification", 
      "linear constructs", 
      "lines", 
      "alternative", 
      "low reactivity", 
      "potential", 
      "comparison", 
      "growth", 
      "thioredoxin gene", 
      "bioconjugates", 
      "library", 
      "vector", 
      "sp", 
      "mode", 
      "frame", 
      "work", 
      "FliTrxTM", 
      "pPROTet", 
      "targeted PACT", 
      "methicillin-resistant Staphylococcus aureus-specific peptides", 
      "Staphylococcus aureus-specific peptides", 
      "aureus-specific peptides"
    ], 
    "name": "Identification of methicillin-resistant Staphylococcus aureus-specific peptides for targeted photoantimicrobial chemotherapy", 
    "pagination": "515-522", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1050163459"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1039/c0pp00267d"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "21180705"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1039/c0pp00267d", 
      "https://app.dimensions.ai/details/publication/pub.1050163459"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:25", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_535.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1039/c0pp00267d"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1039/c0pp00267d'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1039/c0pp00267d'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1039/c0pp00267d'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1039/c0pp00267d'


 

This table displays all metadata directly associated to this object as RDF triples.

264 TRIPLES      22 PREDICATES      120 URIs      104 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1039/c0pp00267d schema:about N03700448e44845f9ab52d08afe6c08cc
2 N79204e6ad0c7452d91cedf91558ed224
3 N8ca3cfd867934218b1f170f638962494
4 N975d5f7f005a4294adf9688f488733fb
5 Na8d24f0583a64bc286aad20c26a05032
6 Na9830d9efacc4b12bcca0e7ea5d69452
7 Ne3d8f2545da74bdc81189b340859f588
8 Ne5367ca0f0454643a09339072e350a87
9 Nee478eba83b14c56a1bd002e48c26b97
10 Nf00076b0956545398af28eebe82eb481
11 anzsrc-for:06
12 anzsrc-for:0604
13 schema:author N31cb49f52b3f4ca59a641654ef8a4af4
14 schema:citation sg:pub.10.1007/s10103-006-0409-7
15 sg:pub.10.1007/s101030050021
16 sg:pub.10.1007/s10434-000-0743-0
17 sg:pub.10.1038/nbt0495-366
18 sg:pub.10.1038/nbt0794-703
19 sg:pub.10.1038/sj.bjc.6602517
20 sg:pub.10.1039/b311900a
21 sg:pub.10.1186/1471-2180-9-27
22 schema:datePublished 2011-04
23 schema:datePublishedReg 2011-04-01
24 schema:description The increasing prevalence of multi-drug resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), necessitates development of alternative modes of bacterial targeting which are not hindered by antibiotic resistance and minimise collateral damage. To achieve this, the FliTrxTM bacterially-displayed peptide library was panned against MRSA and randomly selected clones (n = 20) were DNA sequenced. One selected peptide was synthesised as both cyclic and linear constructs. Binding of the cyclic construct was observed by flow cytometry against isolates of MRSA whilst the linear construct showed low affinity. Low reactivity was observed with other Staphylococcal sp., Gram-negative bacteria and human keratinocytes. The selected peptide was also cloned in-frame, within the thioredoxin gene into the pPROTet.E 6xHN vector for protein expression. A porphyrin photosensitiser (5-(4-isothiocyanatophenyl)-10,15,20-tris(4-N-methylpyridiniumyl)porphyrin trichloride) was conjugated to the recombinant protein and the in vitro cytotoxic effect of the resulting bioconjugate was determined against MRSA and other non-specific bacterial and mammalian cell lines. Photoantimicrobial chemotherapy (PACT) using the bioconjugate showed a 66% reduction in MRSA growth in comparison with non-irradiated cells. This work demonstrates the potential to isolate peptides with binding specificity against MRSA that can be used for targeted PACT, providing an effective alternative to antibody targeting.
25 schema:genre article
26 schema:inLanguage en
27 schema:isAccessibleForFree false
28 schema:isPartOf N5c6cffb7c4da4cf081f108fcfd448bb7
29 N970405fe7f7b44caaf9f377dd620a7c5
30 sg:journal.1029729
31 schema:keywords DNA
32 FliTrxTM
33 Gram-negative bacteria
34 MRSA growth
35 Staphylococcal sp
36 Staphylococcus aureus
37 Staphylococcus aureus-specific peptides
38 affinity
39 alternative
40 alternative modes
41 antibiotic resistance
42 antibodies
43 aureus
44 aureus-specific peptides
45 bacteria
46 bacterial targeting
47 bioconjugates
48 cell lines
49 cells
50 chemotherapy
51 clones
52 collateral damage
53 comparison
54 constructs
55 cyclic constructs
56 cytometry
57 cytotoxic effects
58 damage
59 development
60 effect
61 effective alternative
62 expression
63 flow cytometry
64 frame
65 genes
66 growth
67 human keratinocytes
68 identification
69 isolates
70 isolates of MRSA
71 keratinocytes
72 library
73 linear constructs
74 lines
75 low affinity
76 low reactivity
77 mammalian cell lines
78 methicillin-resistant Staphylococcus aureus
79 methicillin-resistant Staphylococcus aureus-specific peptides
80 minimise collateral damage
81 mode
82 multi-drug resistant bacteria
83 non-irradiated cells
84 pPROTet
85 peptide libraries
86 peptides
87 photoantimicrobial chemotherapy
88 photosensitiser
89 porphyrin photosensitiser
90 potential
91 prevalence
92 protein
93 protein expression
94 reactivity
95 recombinant proteins
96 reduction
97 resistance
98 resistant bacteria
99 sp
100 specificity
101 targeted PACT
102 targeting
103 thioredoxin gene
104 vector
105 work
106 schema:name Identification of methicillin-resistant Staphylococcus aureus-specific peptides for targeted photoantimicrobial chemotherapy
107 schema:pagination 515-522
108 schema:productId Nbe8b15da40be462e8d47b6a912eb844c
109 Nf15ec19e887c4e16ae31d4c8522c2caf
110 Nfb93eff99d23494ca3ae819f4d5d49f7
111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050163459
112 https://doi.org/10.1039/c0pp00267d
113 schema:sdDatePublished 2022-01-01T18:25
114 schema:sdLicense https://scigraph.springernature.com/explorer/license/
115 schema:sdPublisher N83cb040f56944f70bb02195a5bce2a8b
116 schema:url https://doi.org/10.1039/c0pp00267d
117 sgo:license sg:explorer/license/
118 sgo:sdDataset articles
119 rdf:type schema:ScholarlyArticle
120 N03700448e44845f9ab52d08afe6c08cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Humans
122 rdf:type schema:DefinedTerm
123 N110c017425d6444d9353b1bb6bacadc9 rdf:first sg:person.01154124213.03
124 rdf:rest N2968cfa238e84c78bd7f0d0672250400
125 N23a60efaf4c347b48036f5c352517464 schema:affiliation grid-institutes:None
126 schema:familyName Todman
127 schema:givenName Martin
128 rdf:type schema:Person
129 N27c7eff3a353444d958d0e08217ff55b rdf:first sg:person.0754170773.23
130 rdf:rest rdf:nil
131 N2968cfa238e84c78bd7f0d0672250400 rdf:first sg:person.01270352613.45
132 rdf:rest Neec6610034b4452484b5c79638aab3e8
133 N31cb49f52b3f4ca59a641654ef8a4af4 rdf:first sg:person.01170622731.99
134 rdf:rest Ndb757bb16c81492aa5c60460f2cdc1fa
135 N5c6cffb7c4da4cf081f108fcfd448bb7 schema:volumeNumber 10
136 rdf:type schema:PublicationVolume
137 N6c1030d0f97f43348efcaed8483220ad rdf:first sg:person.0663342455.10
138 rdf:rest N110c017425d6444d9353b1bb6bacadc9
139 N79204e6ad0c7452d91cedf91558ed224 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Thioredoxins
141 rdf:type schema:DefinedTerm
142 N79a81843d99c444a8e06d053276a5177 schema:affiliation grid-institutes:grid.9759.2
143 schema:familyName Paget
144 schema:givenName Tim
145 rdf:type schema:Person
146 N83cb040f56944f70bb02195a5bce2a8b schema:name Springer Nature - SN SciGraph project
147 rdf:type schema:Organization
148 N8ca3cfd867934218b1f170f638962494 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Peptides
150 rdf:type schema:DefinedTerm
151 N970405fe7f7b44caaf9f377dd620a7c5 schema:issueNumber 4
152 rdf:type schema:PublicationIssue
153 N975d5f7f005a4294adf9688f488733fb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Recombinant Proteins
155 rdf:type schema:DefinedTerm
156 Na8d24f0583a64bc286aad20c26a05032 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Porphyrins
158 rdf:type schema:DefinedTerm
159 Na9830d9efacc4b12bcca0e7ea5d69452 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Cell Line
161 rdf:type schema:DefinedTerm
162 Nbe8b15da40be462e8d47b6a912eb844c schema:name doi
163 schema:value 10.1039/c0pp00267d
164 rdf:type schema:PropertyValue
165 Nc83385f22d29480387ba82e5152c0363 rdf:first N79a81843d99c444a8e06d053276a5177
166 rdf:rest N27c7eff3a353444d958d0e08217ff55b
167 Ndb757bb16c81492aa5c60460f2cdc1fa rdf:first sg:person.01006261131.17
168 rdf:rest N6c1030d0f97f43348efcaed8483220ad
169 Ne3d8f2545da74bdc81189b340859f588 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Keratinocytes
171 rdf:type schema:DefinedTerm
172 Ne5367ca0f0454643a09339072e350a87 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Photosensitizing Agents
174 rdf:type schema:DefinedTerm
175 Nee478eba83b14c56a1bd002e48c26b97 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Anti-Bacterial Agents
177 rdf:type schema:DefinedTerm
178 Neec6610034b4452484b5c79638aab3e8 rdf:first N23a60efaf4c347b48036f5c352517464
179 rdf:rest Nc83385f22d29480387ba82e5152c0363
180 Nf00076b0956545398af28eebe82eb481 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Methicillin-Resistant Staphylococcus aureus
182 rdf:type schema:DefinedTerm
183 Nf15ec19e887c4e16ae31d4c8522c2caf schema:name pubmed_id
184 schema:value 21180705
185 rdf:type schema:PropertyValue
186 Nfb93eff99d23494ca3ae819f4d5d49f7 schema:name dimensions_id
187 schema:value pub.1050163459
188 rdf:type schema:PropertyValue
189 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
190 schema:name Biological Sciences
191 rdf:type schema:DefinedTerm
192 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
193 schema:name Genetics
194 rdf:type schema:DefinedTerm
195 sg:journal.1029729 schema:issn 1474-905X
196 1474-9092
197 schema:name Photochemical & Photobiological Sciences
198 schema:publisher Springer Nature
199 rdf:type schema:Periodical
200 sg:person.01006261131.17 schema:affiliation grid-institutes:grid.9481.4
201 schema:familyName Madden
202 schema:givenName Leigh A.
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01006261131.17
204 rdf:type schema:Person
205 sg:person.01154124213.03 schema:affiliation grid-institutes:grid.9481.4
206 schema:familyName Savoie
207 schema:givenName Huguette
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154124213.03
209 rdf:type schema:Person
210 sg:person.01170622731.99 schema:affiliation grid-institutes:grid.9481.4
211 schema:familyName Vince
212 schema:givenName Rebecca V.
213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170622731.99
214 rdf:type schema:Person
215 sg:person.01270352613.45 schema:affiliation grid-institutes:grid.9481.4
216 schema:familyName Boyle
217 schema:givenName Ross W.
218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270352613.45
219 rdf:type schema:Person
220 sg:person.0663342455.10 schema:affiliation grid-institutes:grid.9481.4
221 schema:familyName Alonso
222 schema:givenName Cristina M. A.
223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663342455.10
224 rdf:type schema:Person
225 sg:person.0754170773.23 schema:affiliation grid-institutes:grid.9481.4
226 schema:familyName Greenman
227 schema:givenName John
228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754170773.23
229 rdf:type schema:Person
230 sg:pub.10.1007/s10103-006-0409-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044366929
231 https://doi.org/10.1007/s10103-006-0409-7
232 rdf:type schema:CreativeWork
233 sg:pub.10.1007/s101030050021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033947620
234 https://doi.org/10.1007/s101030050021
235 rdf:type schema:CreativeWork
236 sg:pub.10.1007/s10434-000-0743-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006319679
237 https://doi.org/10.1007/s10434-000-0743-0
238 rdf:type schema:CreativeWork
239 sg:pub.10.1038/nbt0495-366 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031929455
240 https://doi.org/10.1038/nbt0495-366
241 rdf:type schema:CreativeWork
242 sg:pub.10.1038/nbt0794-703 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040927659
243 https://doi.org/10.1038/nbt0794-703
244 rdf:type schema:CreativeWork
245 sg:pub.10.1038/sj.bjc.6602517 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016860593
246 https://doi.org/10.1038/sj.bjc.6602517
247 rdf:type schema:CreativeWork
248 sg:pub.10.1039/b311900a schema:sameAs https://app.dimensions.ai/details/publication/pub.1035001576
249 https://doi.org/10.1039/b311900a
250 rdf:type schema:CreativeWork
251 sg:pub.10.1186/1471-2180-9-27 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045409023
252 https://doi.org/10.1186/1471-2180-9-27
253 rdf:type schema:CreativeWork
254 grid-institutes:None schema:alternateName Smith & Nephew Research Centre, YO10 5DF, York, UK
255 schema:name Smith & Nephew Research Centre, YO10 5DF, York, UK
256 rdf:type schema:Organization
257 grid-institutes:grid.9481.4 schema:alternateName Department of Chemistry, The University of Hull, HU6 7RX, UK
258 Postgraduate Medical Institute, The University of Hull, Room 512, Wolfson building, Cottingham Road, HU6 7RX, Hull, UK
259 schema:name Department of Chemistry, The University of Hull, HU6 7RX, UK
260 Postgraduate Medical Institute, The University of Hull, Room 512, Wolfson building, Cottingham Road, HU6 7RX, Hull, UK
261 rdf:type schema:Organization
262 grid-institutes:grid.9759.2 schema:alternateName Medway School of Pharmacy, The University of Kent, ME4 4TB, UK
263 schema:name Medway School of Pharmacy, The University of Kent, ME4 4TB, UK
264 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...