Decreased AGO2 and DCR1 in PBMCs from War Veterans with PTSD leads to diminished miRNA resulting in elevated inflammation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-08-29

AUTHORS

M Bam, X Yang, E E Zumbrun, J P Ginsberg, Q Leyden, J Zhang, P S Nagarkatti, M Nagarkatti

ABSTRACT

Chronic inflammation is a characteristic of post-traumatic stress disorder (PTSD). The initiation of inflammation and molecules involved are not yet clearly understood. Here, we provide compelling evidence that the inflammation seen in PTSD may result from the dysregulated miRNA processing pathway. Using microarray analysis with a discovery group of peripheral blood mononuclear cell (PBMC) samples from War Veterans with PTSD, we found 183 significantly downregulated miRNAs, several of which target numerous genes categorized to be pro-inflammatory in nature. This observation was further confirmed in a replicate group by including more samples. Furthermore, employing RNA-sequencing, quantitative real time PCR (qRT-PCR) and in vitro experiments, we found that Argonaute 2 (AGO2) and Dicer1 (DCR1) were downregulated in PTSD and provided convincing evidence that their downregulation affects mature miRNA generation. In addition, we noted that STAT3 transcript was reduced in PTSD and this was possibly responsible for reduced AGO2 and DCR1, which in turn affected miRNA synthesis. Furthermore, we observed that activation of CD4+ T cells or monocytes led to reduced mature miRNA availability. Finally, the inflammation seen in PTSD was associated with downregulated miRNA profile. Altogether, the current study demonstrates that the chronic inflammation seen in PTSD may be a result of dysregulated miRNA biogenesis pathway due to diminished expression of the key molecules like AGO2, DCR1 and STAT3. More... »

PAGES

e1222

References to SciGraph publications

  • 2013-12-03. Long non-coding RNAs: new players in cell differentiation and development in NATURE REVIEWS GENETICS
  • 2003-07. Transcription regulation and animal diversity in NATURE
  • 2014-04-29. The rise of regulatory RNA in NATURE REVIEWS GENETICS
  • 2012-02-15. Modular regulatory principles of large non-coding RNAs in NATURE
  • 2015-09-01. SKA2 Methylation is associated with Decreased Prefrontal Cortical Thickness and Greater PTSD Severity among Trauma-Exposed Veterans in MOLECULAR PSYCHIATRY
  • 2010-05-02. Transcript assembly and abundance estimation from RNA-Seq reveals thousands of new transcripts and switching among isoforms in NATURE BIOTECHNOLOGY
  • 2013-03-05. Structure and function of long noncoding RNAs in epigenetic regulation in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2010-04-27. A dicer-independent miRNA biogenesis pathway that requires Ago catalysis in NATURE
  • 2013-04-25. TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions in GENOME BIOLOGY
  • 2015-11-20. Evidence for Epigenetic Regulation of Pro-Inflammatory Cytokines, Interleukin-12 and Interferon Gamma, in Peripheral Blood Mononuclear Cells from PTSD Patients in JOURNAL OF NEUROIMMUNE PHARMACOLOGY
  • 2015-12-03. DICER1 and microRNA regulation in post-traumatic stress disorder with comorbid depression in NATURE COMMUNICATIONS
  • 2015-03-10. Gene Networks Specific for Innate Immunity Define Post-Traumatic Stress Disorder in MOLECULAR PSYCHIATRY
  • 2014-09-01. DICER1: mutations, microRNAs and mechanisms in NATURE REVIEWS CANCER
  • 2014-10-28. Expression profiles of long non-coding RNAs located in autoimmune disease-associated regions reveal immune cell-type specificity in GENOME MEDICINE
  • 2013-01-29. Posttraumatic stress disorder is associated with an enhanced spontaneous production of pro-inflammatory cytokines by peripheral blood mononuclear cells in BMC PSYCHIATRY
  • 2016-08-11. Dysregulated immune system networks in war veterans with PTSD is an outcome of altered miRNA expression and DNA methylation in SCIENTIFIC REPORTS
  • 2014-07-16. Regulation of microRNA biogenesis in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2007-01-23. Leukocyte glucocorticoid receptor expression and immunoregulation in veterans with and without post-traumatic stress disorder in MOLECULAR PSYCHIATRY
  • 2016-12-14. Microarray analysis of long noncoding RNA and mRNA expression profiles in human macrophages infected with Mycobacterium tuberculosis in SCIENTIFIC REPORTS
  • 2000-12. STAT proteins: novel molecular targets for cancer drug discovery in ONCOGENE
  • 2007-02. The evolution of gene regulation by transcription factors and microRNAs in NATURE REVIEWS GENETICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/tp.2017.185

    DOI

    http://dx.doi.org/10.1038/tp.2017.185

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1091373442

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/28850112


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/17", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Psychology and Cognitive Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Clinical Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1117", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Public Health and Health Services", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1701", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Psychology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Afghan Campaign 2001-", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Argonaute Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Down-Regulation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "GPI-Linked Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gulf War", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Inflammation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Iraq War, 2003-2011", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Leukocytes, Mononuclear", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "MicroRNAs", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Receptors, Tumor Necrosis Factor, Member 10c", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "STAT3 Transcription Factor", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Stress Disorders, Post-Traumatic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Veterans", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA.", 
              "id": "http://www.grid.ac/institutes/grid.254567.7", 
              "name": [
                "Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bam", 
            "givenName": "M", 
            "id": "sg:person.0677044654.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677044654.22"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "William Jennings Bryan Dorn VA Medical Center, Columbia, SC, USA.", 
              "id": "http://www.grid.ac/institutes/grid.417149.e", 
              "name": [
                "Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA.", 
                "William Jennings Bryan Dorn VA Medical Center, Columbia, SC, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yang", 
            "givenName": "X", 
            "id": "sg:person.01233336052.60", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233336052.60"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Division of Virology, USAMRIID, Fort Detrick, MD, USA.", 
              "id": "http://www.grid.ac/institutes/grid.416900.a", 
              "name": [
                "Division of Virology, USAMRIID, Fort Detrick, MD, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zumbrun", 
            "givenName": "E E", 
            "id": "sg:person.01254532346.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254532346.19"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "William Jennings Bryan Dorn VA Medical Center, Columbia, SC, USA.", 
              "id": "http://www.grid.ac/institutes/grid.417149.e", 
              "name": [
                "William Jennings Bryan Dorn VA Medical Center, Columbia, SC, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ginsberg", 
            "givenName": "J P", 
            "id": "sg:person.01001372513.98", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001372513.98"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "William Jennings Bryan Dorn VA Medical Center, Columbia, SC, USA.", 
              "id": "http://www.grid.ac/institutes/grid.417149.e", 
              "name": [
                "William Jennings Bryan Dorn VA Medical Center, Columbia, SC, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Leyden", 
            "givenName": "Q", 
            "id": "sg:person.01143124273.30", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143124273.30"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Epidemiology and Biostatistics, Arnold School of Public Health, University of South Carolina, Columbia, SC, USA.", 
              "id": "http://www.grid.ac/institutes/grid.254567.7", 
              "name": [
                "Department of Epidemiology and Biostatistics, Arnold School of Public Health, University of South Carolina, Columbia, SC, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "J", 
            "id": "sg:person.0674303713.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0674303713.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA.", 
              "id": "http://www.grid.ac/institutes/grid.254567.7", 
              "name": [
                "Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nagarkatti", 
            "givenName": "P S", 
            "id": "sg:person.0734741117.99", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0734741117.99"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA.", 
              "id": "http://www.grid.ac/institutes/grid.254567.7", 
              "name": [
                "Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nagarkatti", 
            "givenName": "M", 
            "id": "sg:person.01231106421.00", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231106421.00"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nrm3838", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014084316", 
              "https://doi.org/10.1038/nrm3838"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.2480", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051164459", 
              "https://doi.org/10.1038/nsmb.2480"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11481-015-9643-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036324869", 
              "https://doi.org/10.1007/s11481-015-9643-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09092", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018277529", 
              "https://doi.org/10.1038/nature09092"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/mp.2015.9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021847979", 
              "https://doi.org/10.1038/mp.2015.9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.1621", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031035095", 
              "https://doi.org/10.1038/nbt.1621"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrg3606", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005007298", 
              "https://doi.org/10.1038/nrg3606"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.onc.1204086", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015007460", 
              "https://doi.org/10.1038/sj.onc.1204086"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc3802", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014129520", 
              "https://doi.org/10.1038/nrc3802"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.mp.4001934", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016852796", 
              "https://doi.org/10.1038/sj.mp.4001934"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature10887", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049083762", 
              "https://doi.org/10.1038/nature10887"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/mp.2015.134", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048339104", 
              "https://doi.org/10.1038/mp.2015.134"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrg1990", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025508802", 
              "https://doi.org/10.1038/nrg1990"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-244x-13-40", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012478069", 
              "https://doi.org/10.1186/1471-244x-13-40"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms10106", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038079653", 
              "https://doi.org/10.1038/ncomms10106"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature01763", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052601820", 
              "https://doi.org/10.1038/nature01763"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrg3722", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015493329", 
              "https://doi.org/10.1038/nrg3722"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/gb-2013-14-4-r36", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015459845", 
              "https://doi.org/10.1186/gb-2013-14-4-r36"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep31209", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013128062", 
              "https://doi.org/10.1038/srep31209"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13073-014-0088-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037120777", 
              "https://doi.org/10.1186/s13073-014-0088-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep38963", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021031981", 
              "https://doi.org/10.1038/srep38963"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2017-08-29", 
        "datePublishedReg": "2017-08-29", 
        "description": "Chronic inflammation is a characteristic of post-traumatic stress disorder (PTSD). The initiation of inflammation and molecules involved are not yet clearly understood. Here, we provide compelling evidence that the inflammation seen in PTSD may result from the dysregulated miRNA processing pathway. Using microarray analysis with a discovery group of peripheral blood mononuclear cell (PBMC) samples from War Veterans with PTSD, we found 183 significantly downregulated miRNAs, several of which target numerous genes categorized to be pro-inflammatory in nature. This observation was further confirmed in a replicate group by including more samples. Furthermore, employing RNA-sequencing, quantitative real time PCR (qRT-PCR) and in vitro experiments, we found that Argonaute 2 (AGO2) and Dicer1 (DCR1) were downregulated in PTSD and provided convincing evidence that their downregulation affects mature miRNA generation. In addition, we noted that STAT3 transcript was reduced in PTSD and this was possibly responsible for reduced AGO2 and DCR1, which in turn affected miRNA synthesis. Furthermore, we observed that activation of CD4+ T cells or monocytes led to reduced mature miRNA availability. Finally, the inflammation seen in PTSD was associated with downregulated miRNA profile. Altogether, the current study demonstrates that the chronic inflammation seen in PTSD may be a result of dysregulated miRNA biogenesis pathway due to diminished expression of the key molecules like AGO2, DCR1 and STAT3.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/tp.2017.185", 
        "inLanguage": "en", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.6460615", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2465760", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2504124", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2435343", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2437944", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2552509", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1045271", 
            "issn": [
              "2158-3188"
            ], 
            "name": "Translational Psychiatry", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "8", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "7"
          }
        ], 
        "keywords": [
          "post-traumatic stress disorder", 
          "chronic inflammation", 
          "peripheral blood mononuclear cell samples", 
          "mononuclear cell samples", 
          "activation of CD4", 
          "initiation of inflammation", 
          "war veterans", 
          "elevated inflammation", 
          "quantitative real-time PCR", 
          "real-time PCR", 
          "inflammation", 
          "stress disorder", 
          "diminished expression", 
          "Argonaute 2", 
          "time PCR", 
          "key molecules", 
          "miRNA profiles", 
          "DICER1", 
          "microarray analysis", 
          "discovery group", 
          "convincing evidence", 
          "replicate groups", 
          "veterans", 
          "RNA sequencing", 
          "cell samples", 
          "current study", 
          "compelling evidence", 
          "STAT3 transcripts", 
          "processing pathway", 
          "CD4", 
          "group", 
          "PBMC", 
          "monocytes", 
          "pathway", 
          "miRNA generation", 
          "miRNA availability", 
          "numerous genes", 
          "disorders", 
          "evidence", 
          "downregulation", 
          "miRNA processing pathway", 
          "STAT3", 
          "activation", 
          "PCR", 
          "cells", 
          "initiation", 
          "expression", 
          "miRNAs", 
          "miRNA biogenesis pathway", 
          "miRNA", 
          "samples", 
          "more samples", 
          "study", 
          "genes", 
          "transcripts", 
          "profile", 
          "molecules", 
          "addition", 
          "biogenesis pathway", 
          "analysis", 
          "miRNA synthesis", 
          "results", 
          "characteristics", 
          "availability", 
          "observations", 
          "synthesis", 
          "generation", 
          "nature", 
          "experiments", 
          "blood mononuclear cell (PBMC) samples", 
          "turn affected miRNA synthesis", 
          "affected miRNA synthesis", 
          "mature miRNA availability", 
          "downregulated miRNA profile", 
          "diminished miRNA"
        ], 
        "name": "Decreased AGO2 and DCR1 in PBMCs from War Veterans with PTSD leads to diminished miRNA resulting in elevated inflammation", 
        "pagination": "e1222", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1091373442"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/tp.2017.185"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "28850112"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/tp.2017.185", 
          "https://app.dimensions.ai/details/publication/pub.1091373442"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-01-01T18:44", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_738.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/tp.2017.185"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/tp.2017.185'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/tp.2017.185'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/tp.2017.185'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/tp.2017.185'


     

    This table displays all metadata directly associated to this object as RDF triples.

    358 TRIPLES      22 PREDICATES      139 URIs      107 LITERALS      21 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/tp.2017.185 schema:about N008e03ed64bf45f1bfa736b3914acebf
    2 N13b977a1d2e84eec92e048c3a0cd1535
    3 N34f2c59b74aa4387954d056437fc5ce1
    4 N37874193b52c49fda1cac7ca238321c3
    5 N3dbf258669a949a6895ab90ac53c19e9
    6 N406ffb1dbb2b4136b58e6a9c9c13d11c
    7 N49e9b5ff375441f884b1cb3bde1d7495
    8 N6094a35e44a147eaad38a8ddc8039b85
    9 N75dfc0007bfc46b499f6c42714646d0c
    10 N80377558bd0a4ea5848356b6f46ee5b4
    11 N9463d33a70ed49eeb029604b9fcef118
    12 Naa9f758f2c384b33bc4f2c7e0c09d483
    13 Nadac31e66bd849f7aa4181909ce48b2f
    14 Nea799d9298104d5aa5e34531bd4a0cca
    15 anzsrc-for:11
    16 anzsrc-for:1103
    17 anzsrc-for:1117
    18 anzsrc-for:17
    19 anzsrc-for:1701
    20 schema:author N00c181d0a38f495ea8c6e252dbb1d29e
    21 schema:citation sg:pub.10.1007/s11481-015-9643-8
    22 sg:pub.10.1038/mp.2015.134
    23 sg:pub.10.1038/mp.2015.9
    24 sg:pub.10.1038/nature01763
    25 sg:pub.10.1038/nature09092
    26 sg:pub.10.1038/nature10887
    27 sg:pub.10.1038/nbt.1621
    28 sg:pub.10.1038/ncomms10106
    29 sg:pub.10.1038/nrc3802
    30 sg:pub.10.1038/nrg1990
    31 sg:pub.10.1038/nrg3606
    32 sg:pub.10.1038/nrg3722
    33 sg:pub.10.1038/nrm3838
    34 sg:pub.10.1038/nsmb.2480
    35 sg:pub.10.1038/sj.mp.4001934
    36 sg:pub.10.1038/sj.onc.1204086
    37 sg:pub.10.1038/srep31209
    38 sg:pub.10.1038/srep38963
    39 sg:pub.10.1186/1471-244x-13-40
    40 sg:pub.10.1186/gb-2013-14-4-r36
    41 sg:pub.10.1186/s13073-014-0088-0
    42 schema:datePublished 2017-08-29
    43 schema:datePublishedReg 2017-08-29
    44 schema:description Chronic inflammation is a characteristic of post-traumatic stress disorder (PTSD). The initiation of inflammation and molecules involved are not yet clearly understood. Here, we provide compelling evidence that the inflammation seen in PTSD may result from the dysregulated miRNA processing pathway. Using microarray analysis with a discovery group of peripheral blood mononuclear cell (PBMC) samples from War Veterans with PTSD, we found 183 significantly downregulated miRNAs, several of which target numerous genes categorized to be pro-inflammatory in nature. This observation was further confirmed in a replicate group by including more samples. Furthermore, employing RNA-sequencing, quantitative real time PCR (qRT-PCR) and in vitro experiments, we found that Argonaute 2 (AGO2) and Dicer1 (DCR1) were downregulated in PTSD and provided convincing evidence that their downregulation affects mature miRNA generation. In addition, we noted that STAT3 transcript was reduced in PTSD and this was possibly responsible for reduced AGO2 and DCR1, which in turn affected miRNA synthesis. Furthermore, we observed that activation of CD4+ T cells or monocytes led to reduced mature miRNA availability. Finally, the inflammation seen in PTSD was associated with downregulated miRNA profile. Altogether, the current study demonstrates that the chronic inflammation seen in PTSD may be a result of dysregulated miRNA biogenesis pathway due to diminished expression of the key molecules like AGO2, DCR1 and STAT3.
    45 schema:genre article
    46 schema:inLanguage en
    47 schema:isAccessibleForFree true
    48 schema:isPartOf N02b663fb00894c10b41d5bfb9f9bfd8d
    49 N47208ba2c4474ae2953a122b50eff674
    50 sg:journal.1045271
    51 schema:keywords Argonaute 2
    52 CD4
    53 DICER1
    54 PBMC
    55 PCR
    56 RNA sequencing
    57 STAT3
    58 STAT3 transcripts
    59 activation
    60 activation of CD4
    61 addition
    62 affected miRNA synthesis
    63 analysis
    64 availability
    65 biogenesis pathway
    66 blood mononuclear cell (PBMC) samples
    67 cell samples
    68 cells
    69 characteristics
    70 chronic inflammation
    71 compelling evidence
    72 convincing evidence
    73 current study
    74 diminished expression
    75 diminished miRNA
    76 discovery group
    77 disorders
    78 downregulated miRNA profile
    79 downregulation
    80 elevated inflammation
    81 evidence
    82 experiments
    83 expression
    84 generation
    85 genes
    86 group
    87 inflammation
    88 initiation
    89 initiation of inflammation
    90 key molecules
    91 mature miRNA availability
    92 miRNA
    93 miRNA availability
    94 miRNA biogenesis pathway
    95 miRNA generation
    96 miRNA processing pathway
    97 miRNA profiles
    98 miRNA synthesis
    99 miRNAs
    100 microarray analysis
    101 molecules
    102 monocytes
    103 mononuclear cell samples
    104 more samples
    105 nature
    106 numerous genes
    107 observations
    108 pathway
    109 peripheral blood mononuclear cell samples
    110 post-traumatic stress disorder
    111 processing pathway
    112 profile
    113 quantitative real-time PCR
    114 real-time PCR
    115 replicate groups
    116 results
    117 samples
    118 stress disorder
    119 study
    120 synthesis
    121 time PCR
    122 transcripts
    123 turn affected miRNA synthesis
    124 veterans
    125 war veterans
    126 schema:name Decreased AGO2 and DCR1 in PBMCs from War Veterans with PTSD leads to diminished miRNA resulting in elevated inflammation
    127 schema:pagination e1222
    128 schema:productId N1864cc50e6444f0ba50ad766384bb0b9
    129 N2c7af837d945474ebd8b8b348b38c8b5
    130 Nd0488fca6d794c8d8caa67d10dc0afee
    131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091373442
    132 https://doi.org/10.1038/tp.2017.185
    133 schema:sdDatePublished 2022-01-01T18:44
    134 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    135 schema:sdPublisher N0fd4aefd7e224ecfae8ef7075763287c
    136 schema:url https://doi.org/10.1038/tp.2017.185
    137 sgo:license sg:explorer/license/
    138 sgo:sdDataset articles
    139 rdf:type schema:ScholarlyArticle
    140 N008e03ed64bf45f1bfa736b3914acebf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Afghan Campaign 2001-
    142 rdf:type schema:DefinedTerm
    143 N00c181d0a38f495ea8c6e252dbb1d29e rdf:first sg:person.0677044654.22
    144 rdf:rest Nff5cc55795544b5c893da72df9483daa
    145 N02b663fb00894c10b41d5bfb9f9bfd8d schema:issueNumber 8
    146 rdf:type schema:PublicationIssue
    147 N0fd4aefd7e224ecfae8ef7075763287c schema:name Springer Nature - SN SciGraph project
    148 rdf:type schema:Organization
    149 N13b977a1d2e84eec92e048c3a0cd1535 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name Down-Regulation
    151 rdf:type schema:DefinedTerm
    152 N1864cc50e6444f0ba50ad766384bb0b9 schema:name pubmed_id
    153 schema:value 28850112
    154 rdf:type schema:PropertyValue
    155 N2253b12eb4f84811b6d1a2f350bdd3f3 rdf:first sg:person.0734741117.99
    156 rdf:rest N44cd6eb001ee4bb08b64e11bde029c88
    157 N2c7af837d945474ebd8b8b348b38c8b5 schema:name dimensions_id
    158 schema:value pub.1091373442
    159 rdf:type schema:PropertyValue
    160 N34f2c59b74aa4387954d056437fc5ce1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    161 schema:name STAT3 Transcription Factor
    162 rdf:type schema:DefinedTerm
    163 N37874193b52c49fda1cac7ca238321c3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    164 schema:name MicroRNAs
    165 rdf:type schema:DefinedTerm
    166 N3dbf258669a949a6895ab90ac53c19e9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    167 schema:name Argonaute Proteins
    168 rdf:type schema:DefinedTerm
    169 N406ffb1dbb2b4136b58e6a9c9c13d11c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    170 schema:name Gulf War
    171 rdf:type schema:DefinedTerm
    172 N44cd6eb001ee4bb08b64e11bde029c88 rdf:first sg:person.01231106421.00
    173 rdf:rest rdf:nil
    174 N46cbbce7f65a4e5bb300b266adf6401a rdf:first sg:person.01143124273.30
    175 rdf:rest N61e0e7036bb84b63a5a2fd4764bb036c
    176 N47208ba2c4474ae2953a122b50eff674 schema:volumeNumber 7
    177 rdf:type schema:PublicationVolume
    178 N49e9b5ff375441f884b1cb3bde1d7495 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    179 schema:name Iraq War, 2003-2011
    180 rdf:type schema:DefinedTerm
    181 N6094a35e44a147eaad38a8ddc8039b85 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    182 schema:name Humans
    183 rdf:type schema:DefinedTerm
    184 N61e0e7036bb84b63a5a2fd4764bb036c rdf:first sg:person.0674303713.31
    185 rdf:rest N2253b12eb4f84811b6d1a2f350bdd3f3
    186 N75dfc0007bfc46b499f6c42714646d0c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    187 schema:name Receptors, Tumor Necrosis Factor, Member 10c
    188 rdf:type schema:DefinedTerm
    189 N7ef43205ba8c4932a81ee4d666d307d8 rdf:first sg:person.01001372513.98
    190 rdf:rest N46cbbce7f65a4e5bb300b266adf6401a
    191 N80377558bd0a4ea5848356b6f46ee5b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    192 schema:name Veterans
    193 rdf:type schema:DefinedTerm
    194 N9463d33a70ed49eeb029604b9fcef118 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    195 schema:name Stress Disorders, Post-Traumatic
    196 rdf:type schema:DefinedTerm
    197 Naa9f758f2c384b33bc4f2c7e0c09d483 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    198 schema:name Inflammation
    199 rdf:type schema:DefinedTerm
    200 Nadac31e66bd849f7aa4181909ce48b2f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    201 schema:name Leukocytes, Mononuclear
    202 rdf:type schema:DefinedTerm
    203 Ncd544b03868d4b4da15bac85dce670f7 rdf:first sg:person.01254532346.19
    204 rdf:rest N7ef43205ba8c4932a81ee4d666d307d8
    205 Nd0488fca6d794c8d8caa67d10dc0afee schema:name doi
    206 schema:value 10.1038/tp.2017.185
    207 rdf:type schema:PropertyValue
    208 Nea799d9298104d5aa5e34531bd4a0cca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    209 schema:name GPI-Linked Proteins
    210 rdf:type schema:DefinedTerm
    211 Nff5cc55795544b5c893da72df9483daa rdf:first sg:person.01233336052.60
    212 rdf:rest Ncd544b03868d4b4da15bac85dce670f7
    213 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    214 schema:name Medical and Health Sciences
    215 rdf:type schema:DefinedTerm
    216 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
    217 schema:name Clinical Sciences
    218 rdf:type schema:DefinedTerm
    219 anzsrc-for:1117 schema:inDefinedTermSet anzsrc-for:
    220 schema:name Public Health and Health Services
    221 rdf:type schema:DefinedTerm
    222 anzsrc-for:17 schema:inDefinedTermSet anzsrc-for:
    223 schema:name Psychology and Cognitive Sciences
    224 rdf:type schema:DefinedTerm
    225 anzsrc-for:1701 schema:inDefinedTermSet anzsrc-for:
    226 schema:name Psychology
    227 rdf:type schema:DefinedTerm
    228 sg:grant.2435343 http://pending.schema.org/fundedItem sg:pub.10.1038/tp.2017.185
    229 rdf:type schema:MonetaryGrant
    230 sg:grant.2437944 http://pending.schema.org/fundedItem sg:pub.10.1038/tp.2017.185
    231 rdf:type schema:MonetaryGrant
    232 sg:grant.2465760 http://pending.schema.org/fundedItem sg:pub.10.1038/tp.2017.185
    233 rdf:type schema:MonetaryGrant
    234 sg:grant.2504124 http://pending.schema.org/fundedItem sg:pub.10.1038/tp.2017.185
    235 rdf:type schema:MonetaryGrant
    236 sg:grant.2552509 http://pending.schema.org/fundedItem sg:pub.10.1038/tp.2017.185
    237 rdf:type schema:MonetaryGrant
    238 sg:grant.6460615 http://pending.schema.org/fundedItem sg:pub.10.1038/tp.2017.185
    239 rdf:type schema:MonetaryGrant
    240 sg:journal.1045271 schema:issn 2158-3188
    241 schema:name Translational Psychiatry
    242 schema:publisher Springer Nature
    243 rdf:type schema:Periodical
    244 sg:person.01001372513.98 schema:affiliation grid-institutes:grid.417149.e
    245 schema:familyName Ginsberg
    246 schema:givenName J P
    247 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001372513.98
    248 rdf:type schema:Person
    249 sg:person.01143124273.30 schema:affiliation grid-institutes:grid.417149.e
    250 schema:familyName Leyden
    251 schema:givenName Q
    252 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143124273.30
    253 rdf:type schema:Person
    254 sg:person.01231106421.00 schema:affiliation grid-institutes:grid.254567.7
    255 schema:familyName Nagarkatti
    256 schema:givenName M
    257 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231106421.00
    258 rdf:type schema:Person
    259 sg:person.01233336052.60 schema:affiliation grid-institutes:grid.417149.e
    260 schema:familyName Yang
    261 schema:givenName X
    262 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233336052.60
    263 rdf:type schema:Person
    264 sg:person.01254532346.19 schema:affiliation grid-institutes:grid.416900.a
    265 schema:familyName Zumbrun
    266 schema:givenName E E
    267 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01254532346.19
    268 rdf:type schema:Person
    269 sg:person.0674303713.31 schema:affiliation grid-institutes:grid.254567.7
    270 schema:familyName Zhang
    271 schema:givenName J
    272 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0674303713.31
    273 rdf:type schema:Person
    274 sg:person.0677044654.22 schema:affiliation grid-institutes:grid.254567.7
    275 schema:familyName Bam
    276 schema:givenName M
    277 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677044654.22
    278 rdf:type schema:Person
    279 sg:person.0734741117.99 schema:affiliation grid-institutes:grid.254567.7
    280 schema:familyName Nagarkatti
    281 schema:givenName P S
    282 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0734741117.99
    283 rdf:type schema:Person
    284 sg:pub.10.1007/s11481-015-9643-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036324869
    285 https://doi.org/10.1007/s11481-015-9643-8
    286 rdf:type schema:CreativeWork
    287 sg:pub.10.1038/mp.2015.134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048339104
    288 https://doi.org/10.1038/mp.2015.134
    289 rdf:type schema:CreativeWork
    290 sg:pub.10.1038/mp.2015.9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021847979
    291 https://doi.org/10.1038/mp.2015.9
    292 rdf:type schema:CreativeWork
    293 sg:pub.10.1038/nature01763 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052601820
    294 https://doi.org/10.1038/nature01763
    295 rdf:type schema:CreativeWork
    296 sg:pub.10.1038/nature09092 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018277529
    297 https://doi.org/10.1038/nature09092
    298 rdf:type schema:CreativeWork
    299 sg:pub.10.1038/nature10887 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049083762
    300 https://doi.org/10.1038/nature10887
    301 rdf:type schema:CreativeWork
    302 sg:pub.10.1038/nbt.1621 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031035095
    303 https://doi.org/10.1038/nbt.1621
    304 rdf:type schema:CreativeWork
    305 sg:pub.10.1038/ncomms10106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038079653
    306 https://doi.org/10.1038/ncomms10106
    307 rdf:type schema:CreativeWork
    308 sg:pub.10.1038/nrc3802 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014129520
    309 https://doi.org/10.1038/nrc3802
    310 rdf:type schema:CreativeWork
    311 sg:pub.10.1038/nrg1990 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025508802
    312 https://doi.org/10.1038/nrg1990
    313 rdf:type schema:CreativeWork
    314 sg:pub.10.1038/nrg3606 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005007298
    315 https://doi.org/10.1038/nrg3606
    316 rdf:type schema:CreativeWork
    317 sg:pub.10.1038/nrg3722 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015493329
    318 https://doi.org/10.1038/nrg3722
    319 rdf:type schema:CreativeWork
    320 sg:pub.10.1038/nrm3838 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014084316
    321 https://doi.org/10.1038/nrm3838
    322 rdf:type schema:CreativeWork
    323 sg:pub.10.1038/nsmb.2480 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051164459
    324 https://doi.org/10.1038/nsmb.2480
    325 rdf:type schema:CreativeWork
    326 sg:pub.10.1038/sj.mp.4001934 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016852796
    327 https://doi.org/10.1038/sj.mp.4001934
    328 rdf:type schema:CreativeWork
    329 sg:pub.10.1038/sj.onc.1204086 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015007460
    330 https://doi.org/10.1038/sj.onc.1204086
    331 rdf:type schema:CreativeWork
    332 sg:pub.10.1038/srep31209 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013128062
    333 https://doi.org/10.1038/srep31209
    334 rdf:type schema:CreativeWork
    335 sg:pub.10.1038/srep38963 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021031981
    336 https://doi.org/10.1038/srep38963
    337 rdf:type schema:CreativeWork
    338 sg:pub.10.1186/1471-244x-13-40 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012478069
    339 https://doi.org/10.1186/1471-244x-13-40
    340 rdf:type schema:CreativeWork
    341 sg:pub.10.1186/gb-2013-14-4-r36 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015459845
    342 https://doi.org/10.1186/gb-2013-14-4-r36
    343 rdf:type schema:CreativeWork
    344 sg:pub.10.1186/s13073-014-0088-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037120777
    345 https://doi.org/10.1186/s13073-014-0088-0
    346 rdf:type schema:CreativeWork
    347 grid-institutes:grid.254567.7 schema:alternateName Department of Epidemiology and Biostatistics, Arnold School of Public Health, University of South Carolina, Columbia, SC, USA.
    348 Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA.
    349 schema:name Department of Epidemiology and Biostatistics, Arnold School of Public Health, University of South Carolina, Columbia, SC, USA.
    350 Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA.
    351 rdf:type schema:Organization
    352 grid-institutes:grid.416900.a schema:alternateName Division of Virology, USAMRIID, Fort Detrick, MD, USA.
    353 schema:name Division of Virology, USAMRIID, Fort Detrick, MD, USA.
    354 rdf:type schema:Organization
    355 grid-institutes:grid.417149.e schema:alternateName William Jennings Bryan Dorn VA Medical Center, Columbia, SC, USA.
    356 schema:name Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, USA.
    357 William Jennings Bryan Dorn VA Medical Center, Columbia, SC, USA.
    358 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...