Specialized activities and expression differences for Clostridium thermocellum biofilm and planktonic cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Alexandru Dumitrache, Dawn M Klingeman, Jace Natzke, Miguel Rodriguez, Richard J Giannone, Robert L Hettich, Brian H Davison, Steven D Brown

ABSTRACT

Clostridium (Ruminiclostridium) thermocellum is a model organism for its ability to deconstruct plant biomass and convert the cellulose into ethanol. The bacterium forms biofilms adherent to lignocellulosic feedstocks in a continuous cell-monolayer in order to efficiently break down and uptake cellulose hydrolysates. We developed a novel bioreactor design to generate separate sessile and planktonic cell populations for omics studies. Sessile cells had significantly greater expression of genes involved in catabolism of carbohydrates by glycolysis and pyruvate fermentation, ATP generation by proton gradient, the anabolism of proteins and lipids and cellular functions critical for cell division consistent with substrate replete conditions. Planktonic cells had notably higher gene expression for flagellar motility and chemotaxis, cellulosomal cellulases and anchoring scaffoldins, and a range of stress induced homeostasis mechanisms such as oxidative stress protection by antioxidants and flavoprotein co-factors, methionine repair, Fe-S cluster assembly and repair in redox proteins, cell growth control through tRNA thiolation, recovery of damaged DNA by nucleotide excision repair and removal of terminal proteins by proteases. This study demonstrates that microbial attachment to cellulose substrate produces widespread gene expression changes for critical functions of this organism and provides physiological insights for two cells populations relevant for engineering of industrially-ready phenotypes. More... »

PAGES

43583

References to SciGraph publications

  • 2012-12. Proteomic analysis of Clostridium thermocellum core metabolism: relative protein expression profiles and growth phase-dependent changes in protein expression in BMC MICROBIOLOGY
  • 2005-03. Hsp70 chaperones: Cellular functions and molecular mechanism in CELLULAR AND MOLECULAR LIFE SCIENCES
  • 2016-09. Biofilms: an emergent form of bacterial life in NATURE REVIEWS MICROBIOLOGY
  • 2011-12. Transcriptomic analysis of Clostridium thermocellumATCC 27405 cellulose fermentation in BMC MICROBIOLOGY
  • 2015-10. A new perspective on lysogeny: prophages as active regulatory switches of bacteria in NATURE REVIEWS MICROBIOLOGY
  • 2012-12. Transcriptomic and proteomic analyses of Desulfovibrio vulgaris biofilms: Carbon and energy flow contribute to the distinct biofilm growth state in BMC GENOMICS
  • 2014-12. Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia in BIOTECHNOLOGY FOR BIOFUELS
  • 2014-12. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 in GENOME BIOLOGY
  • 2013-12. Clostridium thermocellum transcriptomic profiles after exposure to furfural or heat stress in BIOTECHNOLOGY FOR BIOFUELS
  • 2010-10. Indole Affects Biofilm Formation in Bacteria in INDIAN JOURNAL OF MICROBIOLOGY
  • 2016-12. Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure in BIOTECHNOLOGY FOR BIOFUELS
  • 2013-12. Tracking the cellulolytic activity of Clostridium thermocellum biofilms in BIOTECHNOLOGY FOR BIOFUELS
  • 2012-12. Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress in BMC GENOMICS
  • 2014-03. Biogenesis and functions of bacterial S-layers in NATURE REVIEWS MICROBIOLOGY
  • 2013-12. Global transcriptome analysis of Clostridium thermocellum ATCC 27405 during growth on dilute acid pretreated Populus and switchgrass in BIOTECHNOLOGY FOR BIOFUELS
  • 2016-09. The Perseus computational platform for comprehensive analysis of (prote)omics data in NATURE METHODS
  • 2015-12. Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation in BIOTECHNOLOGY FOR BIOFUELS
  • 2014-12. Transcriptomic analysis of Clostridium thermocellum Populus hydrolysate-tolerant mutant strain shows increased cellular efficiency in response to Populus hydrolysate compared to the wild type strain in BMC MICROBIOLOGY
  • 2015-12. Elimination of hydrogenase active site assembly blocks H2 production and increases ethanol yield in Clostridium thermocellum in BIOTECHNOLOGY FOR BIOFUELS
  • 2016-07-05. Genomics-informed isolation and characterization of a symbiotic Nanoarchaeota system from a terrestrial geothermal environment in NATURE COMMUNICATIONS
  • 2014-12. The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading in BIOTECHNOLOGY FOR BIOFUELS
  • 2012-05. Signaling-mediated bacterial persister formation in NATURE CHEMICAL BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/srep43583

    DOI

    http://dx.doi.org/10.1038/srep43583

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1084131503

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/28240279


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