A smart sealed nucleic acid biosensor based on endogenous reference gene detection to screen and identify mammals on site View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-12

AUTHORS

Yuancong Xu, Wenjin Xiang, Qin Wang, Nan Cheng, Li Zhang, Kunlun Huang, Wentao Xu

ABSTRACT

The identification of meat adulteration is a hotspot for food research worldwide. In this paper, a smart and sealed biosensor that combines loop-mediated isothermal amplification (LAMP) with a lateral flow device (LFD) was developed, resulting in the universal mammalian assessment on site. First, the highly specific chromosomal Glucagon gene (Gcg) was chosen as the endogenous reference gene, and the LAMP approach provided double-labeled duplex DNA products using FITC- and BIO- modified primers. Then, an LFD strategy was used for specific signal recognition through an immunoassay. Meanwhile, LFD-LAMP was compared to LAMP and real-time LAMP, the results showed consistent high specificity and sensitivity but in a more convenient and easy-to-use system. In addition, the detection limit was as low as 10 pg, which was equivalent to 3~5 copies in mammals. All of the reactions were performed in a sealed system regardless of the amplification process or products recognized. Therefore, the smart design demonstrated significantly high specificity and the ability to detect trace amounts of DNA in complex and processed foods with mammalian meat. As a universal and specific platform for the detection of mammalian DNA, this smart biosensor is an excellent prospect for species identification and meat adulteration. More... »

PAGES

43453

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep43453

DOI

http://dx.doi.org/10.1038/srep43453

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1083863162

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28233849


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