Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-12

AUTHORS

Wei Cui, Xiangpeng Dai, Chelsea Marcho, Zhengbin Han, Kun Zhang, Kimberly D Tremblay, Jesse Mager

ABSTRACT

With readily available transcriptome-wide data, understanding the role of each expressed gene is an essential next step. Although RNAi technologies allow for genome-wide screens in cell culture, these approaches cannot replace strategies for discovery in the embryo. Here we present, for the first time, a knockdown screen in mouse preimplantation embryos. Early mammalian development encompasses dynamic cellular, molecular and epigenetic events that are largely conserved from mouse to man. We assayed 712 genes for requirements during preimplantation. We identified 59 genes required for successful development or outgrowth and implantation. We have characterized each phenotype and revealed cellular, molecular, and lineage specific defects following knockdown of transcript. Induced network analyses demonstrate this as a valid approach to identify networks of genes that play important roles during preimplantation. Our approach provides a robust and efficient strategy towards identification of novel phenotypes during mouse preimplantation and facilitates functional annotation of the mammalian transcriptome. More... »

PAGES

37396

References to SciGraph publications

  • 2014-09. The catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes in CELL RESEARCH
  • 2009-02. Making a commitment: cell lineage allocation and axis patterning in the early mouse embryo in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2013-09. An ENU mutagenesis screen identifies novel and known genes involved in epigenetic processes in the mouse in GENOME BIOLOGY
  • 2013-09. Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2015-11. Dynamic expression of chromatin modifiers during developmental transitions in mouse preimplantation embryos in SCIENTIFIC REPORTS
  • 2016-07. HDAC1 and HDAC2 in mouse oocytes and preimplantation embryos: Specificity versus compensation in CELL DEATH & DIFFERENTIATION
  • 1999-10. Disruption of the mouse necdin gene results in early post-natal lethality in NATURE GENETICS
  • 1995-11. Altered Hox expression and segmental identity in Mll-mutant mice in NATURE
  • 2013-03. Gene silencing in tick cell lines using small interfering or long double-stranded RNA in EXPERIMENTAL AND APPLIED ACAROLOGY
  • 2000-02. Specific interference with gene function by double-stranded RNA in early mouse development in NATURE CELL BIOLOGY
  • 2012. Cell Lineage Allocation Within the Inner Cell Mass of the Mouse Blastocyst in MOUSE DEVELOPMENT
  • 2009-07. Making a firm decision: multifaceted regulation of cell fate in the early mouse embryo in NATURE REVIEWS GENETICS
  • 2014-11. Chromatin dynamics in the regulation of cell fate allocation during early embryogenesis in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2008-12. BatchPrimer3: A high throughput web application for PCR and sequencing primer design in BMC BIOINFORMATICS
  • 2015-08. Live imaging RNAi screen reveals genes essential for meiosis in mammalian oocytes in NATURE
  • 2007-12. Mouse preimplantation embryo responses to culture medium osmolarity include increased expression of CCM2 and p38 MAPK activation in BMC DEVELOPMENTAL BIOLOGY
  • 2016-05. DNA methylation dynamics in mouse preimplantation embryos revealed by mass spectrometry in SCIENTIFIC REPORTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/srep37396

    DOI

    http://dx.doi.org/10.1038/srep37396

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1010321426

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27869233


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