Development of functional human oral mucosal epithelial stem/progenitor cell sheets using a feeder-free and serum-free culture system for ocular surface ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-11-14

AUTHORS

Takahiro Nakamura, Seiichi Yokoo, Adams J. Bentley, Maho Nagata, Nigel J. Fullwood, Tsutomu Inatomi, Chie Sotozono, Satoru Yamagami, Shigeru Kinoshita

ABSTRACT

Ocular surface reconstruction (OSR) using tissue-engineered cultivated oral mucosal epithelial cell sheets (COMECS) is a promising newly developed treatment for patients with severe ocular surface disease. Until now, this technique has used exogenic and undefined components such as mouse-derived 3T3 feeder cells and fetal bovine serum. To minimize associated risks of zoonotic infection or transmission of unknown pathogens and so establish a safe and effective protocol for the next generation of treatment modality, we developed a novel technique for the COMECS protocol, using a feeder-free and serum-free (FFSF) culture system. Following this new protocol, COMECS exhibited 4–5 layers of well-stratified and differentiated cells, and we successfully produced functional COMECS that included holoclone-type stem cells. Immunohistochemistry confirmed the presence of markers for cell junction (ZO1, Desmoplakin), basement membrane assembly (Collagen 7, Laminin 5), differentiation (K13, K3), proliferation (Ki67) and stem/progenitor cells (p75) in the FFSF COMECS. When transplanted to the ocular surfaces of rabbits, the tissue survived for up to 2 weeks. This study represents a first step toward assessing the development of functional FFSF COMECS for safe and ideal OSR. More... »

PAGES

37173

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep37173

DOI

http://dx.doi.org/10.1038/srep37173

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1053693382

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27841343


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