Evaluation of the Illumigene Malaria LAMP: A Robust Molecular Diagnostic Tool for Malaria Parasites View Full Text


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Article Info

DATE

2016-12

AUTHORS

Naomi W. Lucchi, Marie Gaye, Mammadou Alpha Diallo, Ira F. Goldman, Dragan Ljolje, Awa Bineta Deme, Aida Badiane, Yaye Die Ndiaye, John W. Barnwell, Venkatachalam Udhayakumar, Daouda Ndiaye

ABSTRACT

Isothermal nucleic acid amplification assays such as the loop mediated isothermal amplification (LAMP), are well suited for field use as they do not require thermal cyclers to amplify the DNA. To further facilitate the use of LAMP assays in remote settings, simpler sample preparation methods and lyophilized reagents are required. The performance of a commercial malaria LAMP assay (Illumigene Malaria LAMP) was evaluated using two sample preparation workflows (simple filtration prep (SFP)) and gravity-driven filtration prep (GFP)) and pre-dispensed lyophilized reagents. Laboratory and clinical samples were tested in a field laboratory in Senegal and the results independently confirmed in a reference laboratory in the U.S.A. The Illumigene Malaria LAMP assay was easily implemented in the clinical laboratory and gave similar results to a real-time PCR reference test with limits of detection of ≤2.0 parasites/μl depending on the sample preparation method used. This assay reliably detected Plasmodium sp. parasites in a simple low-tech format, providing a much needed alternative to the more complex molecular tests for malaria diagnosis. More... »

PAGES

36808

References to SciGraph publications

  • 2015-12. Loop-mediated isothermal amplification (LAMP) for point-of-care detection of asymptomatic low-density malaria parasite carriers in Zanzibar in MALARIA JOURNAL
  • 2014-12. Field evaluation of the photo-induced electron transfer fluorogenic primers (PET) real-time PCR for the detection of Plasmodium falciparum in Tanzania in MALARIA JOURNAL
  • 2013-12. Detection of a substantial number of sub-microscopic Plasmodium falciparum infections by polymerase chain reaction: a potential threat to malaria control and diagnosis in Ethiopia in MALARIA JOURNAL
  • 2010-12. A large proportion of asymptomatic Plasmodium infections with low and sub-microscopic parasite densities in the low transmission setting of Temotu Province, Solomon Islands: challenges for malaria diagnostics in an elimination setting in MALARIA JOURNAL
  • 2012-01. Factors determining the occurrence of submicroscopic malaria infections and their relevance for control in NATURE COMMUNICATIONS
  • 2015-12. Evaluation of non-instrumented nucleic acid amplification by loop-mediated isothermal amplification (NINA-LAMP) for the diagnosis of malaria in Northwest Ethiopia in MALARIA JOURNAL
  • 2011-12. Specific, sensitive and rapid detection of human plasmodium knowlesi infection by loop-mediated isothermal amplification (LAMP) in blood samples in MALARIA JOURNAL
  • 2015-12. Field deployment of loop-mediated isothermal amplification for centralized mass-screening of asymptomatic malaria in Zanzibar: a pre-elimination setting in MALARIA JOURNAL
  • 2015-12. A simple, high-throughput, colourimetric, field applicable loop-mediated isothermal amplification (HtLAMP) assay for malaria elimination in MALARIA JOURNAL
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/srep36808

    DOI

    http://dx.doi.org/10.1038/srep36808

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1039404213

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27827432


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