The primary transcriptome of the Escherichia coli O104:H4 pAA plasmid and novel insights into its virulence gene expression and regulation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-12

AUTHORS

Petya Berger, Michael Knödler, Konrad U Förstner, Michael Berger, Christian Bertling, Cynthia M Sharma, Jörg Vogel, Helge Karch, Ulrich Dobrindt, Alexander Mellmann

ABSTRACT

Escherichia coli O104:H4 (E. coli O104:H4), which caused a massive outbreak of acute gastroenteritis and hemolytic uremic syndrome in 2011, carries an aggregative adherence fimbriae I (AAF/I) encoding virulence plasmid, pAA. The importance of pAA in host-pathogen interaction and disease severity has been demonstrated, however, not much is known about its transcriptional organization and gene regulation. Here, we analyzed the pAA primary transcriptome using differential RNA sequencing, which allows for the high-throughput mapping of transcription start site (TSS) and non-coding RNA candidates. We identified 248 TSS candidates in the 74-kb pAA and only 21% of them could be assigned as TSS of annotated genes. We detected TSS for the majority of pAA-encoded virulence factors. Interestingly, we mapped TSS, which could allow for the transcriptional uncoupling of the AAF/I operon, and potentially regulatory antisense RNA candidates against the genes encoding dispersin and the serine protease SepA. Moreover, a computational search for transcription factor binding sites suggested for AggR-mediated activation of SepA expression, which was additionally experimentally validated. This work advances our understanding of the molecular basis of E. coli O104:H4 pathogenicity and provides a valuable resource for further characterization of pAA virulence gene regulation. More... »

PAGES

35307

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep35307

DOI

http://dx.doi.org/10.1038/srep35307

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050851125

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27748404


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Computational Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli O104", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fimbriae, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Operon", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptide Hydrolases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Antisense", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, RNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Trans-Activators", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcriptome", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Virulence", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of M\u00fcnster", 
          "id": "https://www.grid.ac/institutes/grid.5949.1", 
          "name": [
            "Institute of Hygiene, University of M\u00fcnster, M\u00fcnster, Germany."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Berger", 
        "givenName": "Petya", 
        "id": "sg:person.016605031135.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016605031135.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of M\u00fcnster", 
          "id": "https://www.grid.ac/institutes/grid.5949.1", 
          "name": [
            "Institute of Hygiene, University of M\u00fcnster, M\u00fcnster, Germany."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kn\u00f6dler", 
        "givenName": "Michael", 
        "id": "sg:person.012017755221.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012017755221.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of W\u00fcrzburg", 
          "id": "https://www.grid.ac/institutes/grid.8379.5", 
          "name": [
            "Core Unit Systems Medicine, University of W\u00fcrzburg, W\u00fcrzburg, Germany.", 
            "Institute for Molecular Infection Biology, University of W\u00fcrzburg, W\u00fcrzburg, Germany.", 
            "Research Center for Infectious Diseases, University of W\u00fcrzburg, W\u00fcrzburg, Germany."
          ], 
          "type": "Organization"
        }, 
        "familyName": "F\u00f6rstner", 
        "givenName": "Konrad U", 
        "id": "sg:person.01146220117.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01146220117.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of M\u00fcnster", 
          "id": "https://www.grid.ac/institutes/grid.5949.1", 
          "name": [
            "Institute of Hygiene, University of M\u00fcnster, M\u00fcnster, Germany."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Berger", 
        "givenName": "Michael", 
        "id": "sg:person.012615335621.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012615335621.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of M\u00fcnster", 
          "id": "https://www.grid.ac/institutes/grid.5949.1", 
          "name": [
            "Institute of Hygiene, University of M\u00fcnster, M\u00fcnster, Germany."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bertling", 
        "givenName": "Christian", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of W\u00fcrzburg", 
          "id": "https://www.grid.ac/institutes/grid.8379.5", 
          "name": [
            "Research Center for Infectious Diseases, University of W\u00fcrzburg, W\u00fcrzburg, Germany."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sharma", 
        "givenName": "Cynthia M", 
        "id": "sg:person.01235076504.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235076504.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of W\u00fcrzburg", 
          "id": "https://www.grid.ac/institutes/grid.8379.5", 
          "name": [
            "Institute for Molecular Infection Biology, University of W\u00fcrzburg, W\u00fcrzburg, Germany."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vogel", 
        "givenName": "J\u00f6rg", 
        "id": "sg:person.01042743205.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042743205.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of M\u00fcnster", 
          "id": "https://www.grid.ac/institutes/grid.5949.1", 
          "name": [
            "Institute of Hygiene, University of M\u00fcnster, M\u00fcnster, Germany."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Karch", 
        "givenName": "Helge", 
        "id": "sg:person.01333146377.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01333146377.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of M\u00fcnster", 
          "id": "https://www.grid.ac/institutes/grid.5949.1", 
          "name": [
            "Institute of Hygiene, University of M\u00fcnster, M\u00fcnster, Germany."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dobrindt", 
        "givenName": "Ulrich", 
        "id": "sg:person.0601074636.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601074636.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of M\u00fcnster", 
          "id": "https://www.grid.ac/institutes/grid.5949.1", 
          "name": [
            "Institute of Hygiene, University of M\u00fcnster, M\u00fcnster, Germany."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mellmann", 
        "givenName": "Alexander", 
        "id": "sg:person.01120245034.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01120245034.27"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1371/journal.pgen.1003495", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000952529"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(85)90228-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001967292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa1106920", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002495373"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.02096-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005636901"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0048228", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005907292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature06475", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006278851", 
          "https://doi.org/10.1038/nature06475"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.ge.06.120172.001025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008595197"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/iai.00676-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009533271"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkg694", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011438227"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00330058", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012932753", 
          "https://doi.org/10.1007/bf00330058"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/iai.01310-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013315515"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1914", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013995598", 
          "https://doi.org/10.1038/ng1914"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1914", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013995598", 
          "https://doi.org/10.1038/ng1914"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00261153", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014218131", 
          "https://doi.org/10.1007/bf00261153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00261153", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014218131", 
          "https://doi.org/10.1007/bf00261153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.092759.109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020163602"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2007.05985.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020347015"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0022751", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021924349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1473-3099(11)70165-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021936354"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.01223-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022564448"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.ppat.1004153", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023060866"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-695x.2009.00645.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024932067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0101582", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025094496"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-genet-120213-092340", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025359932"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkq1110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025521671"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mib.2014.06.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026715543"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pcbi.1000502", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026790846"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms4080", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027077596", 
          "https://doi.org/10.1038/ncomms4080"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0066717", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028158685"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.111.089441", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030069403"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkq025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031012343"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.1995.mmi_17010123.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032348989"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkj472", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032807085"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/btr064", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034194664"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/iai.69.1.186-193.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034793857"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/btu533", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035816218"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/btp472", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038248406"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2003-4-1-203", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040835857", 
          "https://doi.org/10.1186/gb-2003-4-1-203"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.2000.01957.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041558710"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mbio.01442-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043195533"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m306413200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044660459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/infdis/jiu399", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044818786"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci16172", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044912289"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00203-011-0725-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046599734", 
          "https://doi.org/10.1007/s00203-011-0725-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa1106483", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047284952"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08756", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050182699", 
          "https://doi.org/10.1038/nature08756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08756", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050182699", 
          "https://doi.org/10.1038/nature08756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/emmm.201201662", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050681879"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkn757", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051916274"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.1991.tb01828.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052789397"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.176.15.4691-4699.1994", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062722906"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074270780", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075788131", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-695x.2010.00645.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078045504"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082424111", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-12", 
    "datePublishedReg": "2016-12-01", 
    "description": "Escherichia coli O104:H4 (E. coli O104:H4), which caused a massive outbreak of acute gastroenteritis and hemolytic uremic syndrome in 2011, carries an aggregative adherence fimbriae I (AAF/I) encoding virulence plasmid, pAA. The importance of pAA in host-pathogen interaction and disease severity has been demonstrated, however, not much is known about its transcriptional organization and gene regulation. Here, we analyzed the pAA primary transcriptome using differential RNA sequencing, which allows for the high-throughput mapping of transcription start site (TSS) and non-coding RNA candidates. We identified 248 TSS candidates in the 74-kb pAA and only 21% of them could be assigned as TSS of annotated genes. We detected TSS for the majority of pAA-encoded virulence factors. Interestingly, we mapped TSS, which could allow for the transcriptional uncoupling of the AAF/I operon, and potentially regulatory antisense RNA candidates against the genes encoding dispersin and the serine protease SepA. Moreover, a computational search for transcription factor binding sites suggested for AggR-mediated activation of SepA expression, which was additionally experimentally validated. This work advances our understanding of the molecular basis of E. coli O104:H4 pathogenicity and provides a valuable resource for further characterization of pAA virulence gene regulation.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/srep35307", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3776294", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3789564", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "6"
      }
    ], 
    "name": "The primary transcriptome of the Escherichia coli O104:H4 pAA plasmid and novel insights into its virulence gene expression and regulation", 
    "pagination": "35307", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "f23c3e548bb87c94329553d4f2b34afcc77a62b9734ddec53aff55127b390acc"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "27748404"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/srep35307"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1050851125"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/srep35307", 
      "https://app.dimensions.ai/details/publication/pub.1050851125"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T21:44", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8687_00000551.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/srep/2016/161017/srep35307/full/srep35307.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/srep35307'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/srep35307'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/srep35307'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/srep35307'


 

This table displays all metadata directly associated to this object as RDF triples.

352 TRIPLES      21 PREDICATES      94 URIs      34 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/srep35307 schema:about N01edfe38c91848cfb87c4d42288c2924
2 N0c713a87ec734aa98bdd75f4f696e0e9
3 N3ca8584209604606ab9522eb06b952f0
4 N4815b55f457041c88f20b3c7b0f7de7a
5 N7632b019efa24c3995f8e4fd788a9571
6 N98244f5653c7482898a8d96a959630e4
7 N9f7e9c08717b4306ba5a3a49cb67bab4
8 Na06d920799a64cd7bb117634c1b27dc8
9 Nbedb8a0054874965a67f4ad1630ef090
10 Nc13b6c2707bf431da891f78127c43a7d
11 Nd41d8ab8458e4934bf22e22057b66b8e
12 Nd659f02fda8448a98ebd0f328e1f249a
13 Ne9c59e35cb894c2e8282d64050c732d7
14 anzsrc-for:06
15 anzsrc-for:0604
16 schema:author N7ab08700f6d545588794e76f21f3c573
17 schema:citation sg:pub.10.1007/bf00261153
18 sg:pub.10.1007/bf00330058
19 sg:pub.10.1007/s00203-011-0725-6
20 sg:pub.10.1038/nature06475
21 sg:pub.10.1038/nature08756
22 sg:pub.10.1038/ncomms4080
23 sg:pub.10.1038/ng1914
24 sg:pub.10.1186/gb-2003-4-1-203
25 https://app.dimensions.ai/details/publication/pub.1074270780
26 https://app.dimensions.ai/details/publication/pub.1075788131
27 https://app.dimensions.ai/details/publication/pub.1082424111
28 https://doi.org/10.1002/emmm.201201662
29 https://doi.org/10.1016/0022-2836(85)90228-1
30 https://doi.org/10.1016/j.mib.2014.06.010
31 https://doi.org/10.1016/s1473-3099(11)70165-7
32 https://doi.org/10.1046/j.1365-2958.2000.01957.x
33 https://doi.org/10.1056/nejmoa1106483
34 https://doi.org/10.1056/nejmoa1106920
35 https://doi.org/10.1074/jbc.m306413200
36 https://doi.org/10.1093/bioinformatics/btp472
37 https://doi.org/10.1093/bioinformatics/btr064
38 https://doi.org/10.1093/bioinformatics/btu533
39 https://doi.org/10.1093/infdis/jiu399
40 https://doi.org/10.1093/nar/gkg694
41 https://doi.org/10.1093/nar/gkj472
42 https://doi.org/10.1093/nar/gkn757
43 https://doi.org/10.1093/nar/gkq025
44 https://doi.org/10.1093/nar/gkq1110
45 https://doi.org/10.1101/gr.092759.109
46 https://doi.org/10.1105/tpc.111.089441
47 https://doi.org/10.1111/j.1365-2958.1991.tb01828.x
48 https://doi.org/10.1111/j.1365-2958.1995.mmi_17010123.x
49 https://doi.org/10.1111/j.1365-2958.2007.05985.x
50 https://doi.org/10.1111/j.1574-695x.2009.00645.x
51 https://doi.org/10.1111/j.1574-695x.2010.00645.x
52 https://doi.org/10.1128/iai.00676-12
53 https://doi.org/10.1128/iai.01310-12
54 https://doi.org/10.1128/iai.69.1.186-193.2001
55 https://doi.org/10.1128/jb.01223-12
56 https://doi.org/10.1128/jb.02096-14
57 https://doi.org/10.1128/jb.176.15.4691-4699.1994
58 https://doi.org/10.1128/mbio.01442-14
59 https://doi.org/10.1146/annurev-genet-120213-092340
60 https://doi.org/10.1146/annurev.ge.06.120172.001025
61 https://doi.org/10.1172/jci16172
62 https://doi.org/10.1371/journal.pcbi.1000502
63 https://doi.org/10.1371/journal.pgen.1003495
64 https://doi.org/10.1371/journal.pone.0022751
65 https://doi.org/10.1371/journal.pone.0048228
66 https://doi.org/10.1371/journal.pone.0066717
67 https://doi.org/10.1371/journal.pone.0101582
68 https://doi.org/10.1371/journal.ppat.1004153
69 schema:datePublished 2016-12
70 schema:datePublishedReg 2016-12-01
71 schema:description Escherichia coli O104:H4 (E. coli O104:H4), which caused a massive outbreak of acute gastroenteritis and hemolytic uremic syndrome in 2011, carries an aggregative adherence fimbriae I (AAF/I) encoding virulence plasmid, pAA. The importance of pAA in host-pathogen interaction and disease severity has been demonstrated, however, not much is known about its transcriptional organization and gene regulation. Here, we analyzed the pAA primary transcriptome using differential RNA sequencing, which allows for the high-throughput mapping of transcription start site (TSS) and non-coding RNA candidates. We identified 248 TSS candidates in the 74-kb pAA and only 21% of them could be assigned as TSS of annotated genes. We detected TSS for the majority of pAA-encoded virulence factors. Interestingly, we mapped TSS, which could allow for the transcriptional uncoupling of the AAF/I operon, and potentially regulatory antisense RNA candidates against the genes encoding dispersin and the serine protease SepA. Moreover, a computational search for transcription factor binding sites suggested for AggR-mediated activation of SepA expression, which was additionally experimentally validated. This work advances our understanding of the molecular basis of E. coli O104:H4 pathogenicity and provides a valuable resource for further characterization of pAA virulence gene regulation.
72 schema:genre research_article
73 schema:inLanguage en
74 schema:isAccessibleForFree true
75 schema:isPartOf N2597e4e6d646459083012e65199c895b
76 N919c7390a6a84ba59a76c98afc9235bc
77 sg:journal.1045337
78 schema:name The primary transcriptome of the Escherichia coli O104:H4 pAA plasmid and novel insights into its virulence gene expression and regulation
79 schema:pagination 35307
80 schema:productId N314ebded7e3048eea82bc2990b623581
81 N3730b2ff78014cd2842bbe108d81e82b
82 N77dab8b506bc42ac9c8a01cbfac4911e
83 N99afabac22014528b527c247a188d0cf
84 Nc11b7223651444aa8641f4b5044d7b3e
85 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050851125
86 https://doi.org/10.1038/srep35307
87 schema:sdDatePublished 2019-04-10T21:44
88 schema:sdLicense https://scigraph.springernature.com/explorer/license/
89 schema:sdPublisher Nf9367ad5797d4729bc4cf4e2d061990f
90 schema:url http://www.nature.com/srep/2016/161017/srep35307/full/srep35307.html
91 sgo:license sg:explorer/license/
92 sgo:sdDataset articles
93 rdf:type schema:ScholarlyArticle
94 N01edfe38c91848cfb87c4d42288c2924 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Computational Biology
96 rdf:type schema:DefinedTerm
97 N0c713a87ec734aa98bdd75f4f696e0e9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name RNA, Antisense
99 rdf:type schema:DefinedTerm
100 N237e3d372a5648628fae50af9cf02f1f rdf:first sg:person.01120245034.27
101 rdf:rest rdf:nil
102 N2597e4e6d646459083012e65199c895b schema:volumeNumber 6
103 rdf:type schema:PublicationVolume
104 N275aa04f79c34c52bfede7206b36fa66 rdf:first sg:person.01146220117.41
105 rdf:rest N45b5b267fc484565bd18fe24b777f9ee
106 N314ebded7e3048eea82bc2990b623581 schema:name doi
107 schema:value 10.1038/srep35307
108 rdf:type schema:PropertyValue
109 N3730b2ff78014cd2842bbe108d81e82b schema:name readcube_id
110 schema:value f23c3e548bb87c94329553d4f2b34afcc77a62b9734ddec53aff55127b390acc
111 rdf:type schema:PropertyValue
112 N3ca8584209604606ab9522eb06b952f0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Peptide Hydrolases
114 rdf:type schema:DefinedTerm
115 N45b5b267fc484565bd18fe24b777f9ee rdf:first sg:person.012615335621.24
116 rdf:rest N515bb4ae47d54fc7a30caf411e1e81d3
117 N4815b55f457041c88f20b3c7b0f7de7a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Trans-Activators
119 rdf:type schema:DefinedTerm
120 N515bb4ae47d54fc7a30caf411e1e81d3 rdf:first N92894a08f0ac4d60967999da20813626
121 rdf:rest Nd942b9d24623455d87416baa8778119b
122 N7632b019efa24c3995f8e4fd788a9571 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Sequence Analysis, RNA
124 rdf:type schema:DefinedTerm
125 N77dab8b506bc42ac9c8a01cbfac4911e schema:name dimensions_id
126 schema:value pub.1050851125
127 rdf:type schema:PropertyValue
128 N7ab08700f6d545588794e76f21f3c573 rdf:first sg:person.016605031135.12
129 rdf:rest Ne81a104f376544b09ce3e789f4943151
130 N7d044475c83a4c758425fa0bd31d0916 rdf:first sg:person.0601074636.51
131 rdf:rest N237e3d372a5648628fae50af9cf02f1f
132 N919c7390a6a84ba59a76c98afc9235bc schema:issueNumber 1
133 rdf:type schema:PublicationIssue
134 N92894a08f0ac4d60967999da20813626 schema:affiliation https://www.grid.ac/institutes/grid.5949.1
135 schema:familyName Bertling
136 schema:givenName Christian
137 rdf:type schema:Person
138 N98244f5653c7482898a8d96a959630e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Escherichia coli O104
140 rdf:type schema:DefinedTerm
141 N99afabac22014528b527c247a188d0cf schema:name nlm_unique_id
142 schema:value 101563288
143 rdf:type schema:PropertyValue
144 N9f7e9c08717b4306ba5a3a49cb67bab4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Transcriptome
146 rdf:type schema:DefinedTerm
147 Na06d920799a64cd7bb117634c1b27dc8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Plasmids
149 rdf:type schema:DefinedTerm
150 Nbedb8a0054874965a67f4ad1630ef090 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Operon
152 rdf:type schema:DefinedTerm
153 Nc11b7223651444aa8641f4b5044d7b3e schema:name pubmed_id
154 schema:value 27748404
155 rdf:type schema:PropertyValue
156 Nc13b6c2707bf431da891f78127c43a7d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Virulence
158 rdf:type schema:DefinedTerm
159 Nc1baf7158b92410883e3b12a61f42a6d rdf:first sg:person.01042743205.16
160 rdf:rest Nc6cf5ab907ae42a7bb59b7b858bc5a33
161 Nc6cf5ab907ae42a7bb59b7b858bc5a33 rdf:first sg:person.01333146377.00
162 rdf:rest N7d044475c83a4c758425fa0bd31d0916
163 Nd41d8ab8458e4934bf22e22057b66b8e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Gene Expression Regulation, Bacterial
165 rdf:type schema:DefinedTerm
166 Nd659f02fda8448a98ebd0f328e1f249a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Escherichia coli Proteins
168 rdf:type schema:DefinedTerm
169 Nd942b9d24623455d87416baa8778119b rdf:first sg:person.01235076504.09
170 rdf:rest Nc1baf7158b92410883e3b12a61f42a6d
171 Ne81a104f376544b09ce3e789f4943151 rdf:first sg:person.012017755221.45
172 rdf:rest N275aa04f79c34c52bfede7206b36fa66
173 Ne9c59e35cb894c2e8282d64050c732d7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
174 schema:name Fimbriae, Bacterial
175 rdf:type schema:DefinedTerm
176 Nf9367ad5797d4729bc4cf4e2d061990f schema:name Springer Nature - SN SciGraph project
177 rdf:type schema:Organization
178 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
179 schema:name Biological Sciences
180 rdf:type schema:DefinedTerm
181 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
182 schema:name Genetics
183 rdf:type schema:DefinedTerm
184 sg:grant.3776294 http://pending.schema.org/fundedItem sg:pub.10.1038/srep35307
185 rdf:type schema:MonetaryGrant
186 sg:grant.3789564 http://pending.schema.org/fundedItem sg:pub.10.1038/srep35307
187 rdf:type schema:MonetaryGrant
188 sg:journal.1045337 schema:issn 2045-2322
189 schema:name Scientific Reports
190 rdf:type schema:Periodical
191 sg:person.01042743205.16 schema:affiliation https://www.grid.ac/institutes/grid.8379.5
192 schema:familyName Vogel
193 schema:givenName Jörg
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042743205.16
195 rdf:type schema:Person
196 sg:person.01120245034.27 schema:affiliation https://www.grid.ac/institutes/grid.5949.1
197 schema:familyName Mellmann
198 schema:givenName Alexander
199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01120245034.27
200 rdf:type schema:Person
201 sg:person.01146220117.41 schema:affiliation https://www.grid.ac/institutes/grid.8379.5
202 schema:familyName Förstner
203 schema:givenName Konrad U
204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01146220117.41
205 rdf:type schema:Person
206 sg:person.012017755221.45 schema:affiliation https://www.grid.ac/institutes/grid.5949.1
207 schema:familyName Knödler
208 schema:givenName Michael
209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012017755221.45
210 rdf:type schema:Person
211 sg:person.01235076504.09 schema:affiliation https://www.grid.ac/institutes/grid.8379.5
212 schema:familyName Sharma
213 schema:givenName Cynthia M
214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235076504.09
215 rdf:type schema:Person
216 sg:person.012615335621.24 schema:affiliation https://www.grid.ac/institutes/grid.5949.1
217 schema:familyName Berger
218 schema:givenName Michael
219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012615335621.24
220 rdf:type schema:Person
221 sg:person.01333146377.00 schema:affiliation https://www.grid.ac/institutes/grid.5949.1
222 schema:familyName Karch
223 schema:givenName Helge
224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01333146377.00
225 rdf:type schema:Person
226 sg:person.016605031135.12 schema:affiliation https://www.grid.ac/institutes/grid.5949.1
227 schema:familyName Berger
228 schema:givenName Petya
229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016605031135.12
230 rdf:type schema:Person
231 sg:person.0601074636.51 schema:affiliation https://www.grid.ac/institutes/grid.5949.1
232 schema:familyName Dobrindt
233 schema:givenName Ulrich
234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601074636.51
235 rdf:type schema:Person
236 sg:pub.10.1007/bf00261153 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014218131
237 https://doi.org/10.1007/bf00261153
238 rdf:type schema:CreativeWork
239 sg:pub.10.1007/bf00330058 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012932753
240 https://doi.org/10.1007/bf00330058
241 rdf:type schema:CreativeWork
242 sg:pub.10.1007/s00203-011-0725-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046599734
243 https://doi.org/10.1007/s00203-011-0725-6
244 rdf:type schema:CreativeWork
245 sg:pub.10.1038/nature06475 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006278851
246 https://doi.org/10.1038/nature06475
247 rdf:type schema:CreativeWork
248 sg:pub.10.1038/nature08756 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050182699
249 https://doi.org/10.1038/nature08756
250 rdf:type schema:CreativeWork
251 sg:pub.10.1038/ncomms4080 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027077596
252 https://doi.org/10.1038/ncomms4080
253 rdf:type schema:CreativeWork
254 sg:pub.10.1038/ng1914 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013995598
255 https://doi.org/10.1038/ng1914
256 rdf:type schema:CreativeWork
257 sg:pub.10.1186/gb-2003-4-1-203 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040835857
258 https://doi.org/10.1186/gb-2003-4-1-203
259 rdf:type schema:CreativeWork
260 https://app.dimensions.ai/details/publication/pub.1074270780 schema:CreativeWork
261 https://app.dimensions.ai/details/publication/pub.1075788131 schema:CreativeWork
262 https://app.dimensions.ai/details/publication/pub.1082424111 schema:CreativeWork
263 https://doi.org/10.1002/emmm.201201662 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050681879
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1016/0022-2836(85)90228-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001967292
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1016/j.mib.2014.06.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026715543
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1016/s1473-3099(11)70165-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021936354
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1046/j.1365-2958.2000.01957.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1041558710
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1056/nejmoa1106483 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047284952
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1056/nejmoa1106920 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002495373
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1074/jbc.m306413200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044660459
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1093/bioinformatics/btp472 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038248406
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1093/bioinformatics/btr064 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034194664
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1093/bioinformatics/btu533 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035816218
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1093/infdis/jiu399 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044818786
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1093/nar/gkg694 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011438227
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1093/nar/gkj472 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032807085
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1093/nar/gkn757 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051916274
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1093/nar/gkq025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031012343
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1093/nar/gkq1110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025521671
296 rdf:type schema:CreativeWork
297 https://doi.org/10.1101/gr.092759.109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020163602
298 rdf:type schema:CreativeWork
299 https://doi.org/10.1105/tpc.111.089441 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030069403
300 rdf:type schema:CreativeWork
301 https://doi.org/10.1111/j.1365-2958.1991.tb01828.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1052789397
302 rdf:type schema:CreativeWork
303 https://doi.org/10.1111/j.1365-2958.1995.mmi_17010123.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1032348989
304 rdf:type schema:CreativeWork
305 https://doi.org/10.1111/j.1365-2958.2007.05985.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1020347015
306 rdf:type schema:CreativeWork
307 https://doi.org/10.1111/j.1574-695x.2009.00645.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024932067
308 rdf:type schema:CreativeWork
309 https://doi.org/10.1111/j.1574-695x.2010.00645.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1078045504
310 rdf:type schema:CreativeWork
311 https://doi.org/10.1128/iai.00676-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009533271
312 rdf:type schema:CreativeWork
313 https://doi.org/10.1128/iai.01310-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013315515
314 rdf:type schema:CreativeWork
315 https://doi.org/10.1128/iai.69.1.186-193.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034793857
316 rdf:type schema:CreativeWork
317 https://doi.org/10.1128/jb.01223-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022564448
318 rdf:type schema:CreativeWork
319 https://doi.org/10.1128/jb.02096-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005636901
320 rdf:type schema:CreativeWork
321 https://doi.org/10.1128/jb.176.15.4691-4699.1994 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062722906
322 rdf:type schema:CreativeWork
323 https://doi.org/10.1128/mbio.01442-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043195533
324 rdf:type schema:CreativeWork
325 https://doi.org/10.1146/annurev-genet-120213-092340 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025359932
326 rdf:type schema:CreativeWork
327 https://doi.org/10.1146/annurev.ge.06.120172.001025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008595197
328 rdf:type schema:CreativeWork
329 https://doi.org/10.1172/jci16172 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044912289
330 rdf:type schema:CreativeWork
331 https://doi.org/10.1371/journal.pcbi.1000502 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026790846
332 rdf:type schema:CreativeWork
333 https://doi.org/10.1371/journal.pgen.1003495 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000952529
334 rdf:type schema:CreativeWork
335 https://doi.org/10.1371/journal.pone.0022751 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021924349
336 rdf:type schema:CreativeWork
337 https://doi.org/10.1371/journal.pone.0048228 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005907292
338 rdf:type schema:CreativeWork
339 https://doi.org/10.1371/journal.pone.0066717 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028158685
340 rdf:type schema:CreativeWork
341 https://doi.org/10.1371/journal.pone.0101582 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025094496
342 rdf:type schema:CreativeWork
343 https://doi.org/10.1371/journal.ppat.1004153 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023060866
344 rdf:type schema:CreativeWork
345 https://www.grid.ac/institutes/grid.5949.1 schema:alternateName University of Münster
346 schema:name Institute of Hygiene, University of Münster, Münster, Germany.
347 rdf:type schema:Organization
348 https://www.grid.ac/institutes/grid.8379.5 schema:alternateName University of Würzburg
349 schema:name Core Unit Systems Medicine, University of Würzburg, Würzburg, Germany.
350 Institute for Molecular Infection Biology, University of Würzburg, Würzburg, Germany.
351 Research Center for Infectious Diseases, University of Würzburg, Würzburg, Germany.
352 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...