Structural basis for disruption of claudin assembly in tight junctions by an enterotoxin View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-12

AUTHORS

Takehiro Shinoda, Naoko Shinya, Kaori Ito, Noboru Ohsawa, Takaho Terada, Kunio Hirata, Yoshiaki Kawano, Masaki Yamamoto, Tomomi Kimura-Someya, Shigeyuki Yokoyama, Mikako Shirouzu

ABSTRACT

The food-poisoning bacterium Clostridium perfringens produces an enterotoxin (~35 kDa) that specifically targets human claudin-4, among the 26 human claudin proteins, and causes diarrhea by fluid accumulation in the intestinal cavity. The C-terminal domain of the Clostridium perfringens enterotoxin (C-CPE, ~15 kDa) binds tightly to claudin-4, and disrupts the intestinal tight junction barriers. In this study, we determined the 3.5-Å resolution crystal structure of the cell-free synthesized human claudin-4•C-CPE complex, which is significantly different from the structure of the off-target complex of an engineered C-CPE with mouse claudin-19. The claudin-4•C-CPE complex structure demonstrated the mechanism underlying claudin assembly disruption. A comparison of the present C-CPE-bound structure of claudin-4 with the enterotoxin-free claudin-15 structure revealed sophisticated C-CPE-induced conformation changes of the extracellular segments, induced on the foundation of the rigid four-transmembrane-helix bundle structure. These conformation changes provide a mechanistic model for the disruption of the lateral assembly of claudin molecules. Furthermore, the present novel structural mechanism for selecting a specific member of the claudin family can be used as the foundation to develop novel medically important technologies to selectively regulate the tight junctions formed by claudin family members in different organs. More... »

PAGES

33632

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep33632

DOI

http://dx.doi.org/10.1038/srep33632

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009437358

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27647526


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Claudins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enterotoxins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrophobic and Hydrophilic Interactions", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Biological", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Conformation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Structure-Activity Relationship", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tight Junctions", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "RIKEN", 
          "id": "https://www.grid.ac/institutes/grid.7597.c", 
          "name": [
            "RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.", 
            "Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shinoda", 
        "givenName": "Takehiro", 
        "id": "sg:person.0762157770.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762157770.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "RIKEN", 
          "id": "https://www.grid.ac/institutes/grid.7597.c", 
          "name": [
            "RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.", 
            "Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shinya", 
        "givenName": "Naoko", 
        "id": "sg:person.01117656406.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01117656406.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "RIKEN", 
          "id": "https://www.grid.ac/institutes/grid.7597.c", 
          "name": [
            "RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.", 
            "Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ito", 
        "givenName": "Kaori", 
        "id": "sg:person.01303771126.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303771126.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "RIKEN", 
          "id": "https://www.grid.ac/institutes/grid.7597.c", 
          "name": [
            "RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.", 
            "Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ohsawa", 
        "givenName": "Noboru", 
        "id": "sg:person.01071370106.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071370106.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "RIKEN", 
          "id": "https://www.grid.ac/institutes/grid.7597.c", 
          "name": [
            "RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.", 
            "RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Terada", 
        "givenName": "Takaho", 
        "id": "sg:person.013626202062.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013626202062.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "RIKEN SPring-8 Center, 1-1-1, Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hirata", 
        "givenName": "Kunio", 
        "id": "sg:person.01256470305.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256470305.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "RIKEN SPring-8 Center, 1-1-1, Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kawano", 
        "givenName": "Yoshiaki", 
        "id": "sg:person.0756317004.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0756317004.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "RIKEN SPring-8 Center, 1-1-1, Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yamamoto", 
        "givenName": "Masaki", 
        "id": "sg:person.012771421304.81", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012771421304.81"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "RIKEN", 
          "id": "https://www.grid.ac/institutes/grid.7597.c", 
          "name": [
            "RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.", 
            "Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kimura-Someya", 
        "givenName": "Tomomi", 
        "id": "sg:person.01104104440.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01104104440.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "RIKEN", 
          "id": "https://www.grid.ac/institutes/grid.7597.c", 
          "name": [
            "RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.", 
            "RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yokoyama", 
        "givenName": "Shigeyuki", 
        "id": "sg:person.0723444525.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0723444525.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "RIKEN", 
          "id": "https://www.grid.ac/institutes/grid.7597.c", 
          "name": [
            "RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.", 
            "Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shirouzu", 
        "givenName": "Mikako", 
        "id": "sg:person.01274360652.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274360652.26"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1107/s0907444910007493", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000235100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444910007493", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000235100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.181342398", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006228920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcc.20084", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008225564"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2011.05.161", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011189595"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0021889807021206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012753217"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00018-014-1761-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014596807", 
          "https://doi.org/10.1007/s00018-014-1761-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/toxins2061336", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015107442"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444909042073", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018458004"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444909042073", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018458004"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444998003254", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019779527"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444909052925", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020777046"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444909052925", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020777046"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1085/jgp.200810154", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022094485"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/b:jsfg.0000029204.57846.7d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022949189", 
          "https://doi.org/10.1023/b:jsfg.0000029204.57846.7d"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81553-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025140525"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.136.6.1239", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027081156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(01)00200-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029078331"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep30442", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029400865", 
          "https://doi.org/10.1038/srep30442"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamem.2007.10.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033419088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/j.gastro.2004.07.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036459927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bcp.2007.12.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038183210"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m111.228478", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040355978"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1088/1742-6596/425/1/012002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044156712"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.141.7.1539", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044261156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200302070", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045853086"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m109.043752", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046559787"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200110122", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047702118"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.147.1.195", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048718676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.147.1.195", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048718676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gku340", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052806377"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mbio.00594-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053721695"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/508617", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058786372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1248571", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062469262"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1261833", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062470874"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.285.5424.103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062565757"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/tisb.25247", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072311074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.3463362", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1098526006"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-12", 
    "datePublishedReg": "2016-12-01", 
    "description": "The food-poisoning bacterium Clostridium perfringens produces an enterotoxin (~35\u2009kDa) that specifically targets human claudin-4, among the 26 human claudin proteins, and causes diarrhea by fluid accumulation in the intestinal cavity. The C-terminal domain of the Clostridium perfringens enterotoxin (C-CPE, ~15\u2009kDa) binds tightly to claudin-4, and disrupts the intestinal tight junction barriers. In this study, we determined the 3.5-\u00c5 resolution crystal structure of the cell-free synthesized human claudin-4\u2022C-CPE complex, which is significantly different from the structure of the off-target complex of an engineered C-CPE with mouse claudin-19. The claudin-4\u2022C-CPE complex structure demonstrated the mechanism underlying claudin assembly disruption. A comparison of the present C-CPE-bound structure of claudin-4 with the enterotoxin-free claudin-15 structure revealed sophisticated C-CPE-induced conformation changes of the extracellular segments, induced on the foundation of the rigid four-transmembrane-helix bundle structure. These conformation changes provide a mechanistic model for the disruption of the lateral assembly of claudin molecules. Furthermore, the present novel structural mechanism for selecting a specific member of the claudin family can be used as the foundation to develop novel medically important technologies to selectively regulate the tight junctions formed by claudin family members in different organs.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/srep33632", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.6129713", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "6"
      }
    ], 
    "name": "Structural basis for disruption of claudin assembly in tight junctions by an enterotoxin", 
    "pagination": "33632", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "2cf4e9e1f99737a20066b935313905294cc5639e208612867fec5f918545d379"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "27647526"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/srep33632"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1009437358"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/srep33632", 
      "https://app.dimensions.ai/details/publication/pub.1009437358"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T19:15", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8678_00000549.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/srep/2016/160920/srep33632/full/srep33632.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/srep33632'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/srep33632'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/srep33632'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/srep33632'


 

This table displays all metadata directly associated to this object as RDF triples.

313 TRIPLES      21 PREDICATES      78 URIs      36 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/srep33632 schema:about N10fc1b7b6616437297ddb6da91bfaa7b
2 N141b3dc83cf14d6d9f3186d81987ee6e
3 N2b727aeb13fc42be8fe53d621ac37efe
4 N2dd8eeccc65d4ba0aeaca13145a4121b
5 N34e971cb7d0f43bfb27db569393f0058
6 N3b44396437ea4c98a3a13533fd9aedf0
7 N5cfd70adb9a840028077537b7d6f35ce
8 N6bcd4c212e8d497a98452729ded6504a
9 N8320ae7e4a6146f7abc971aef170d137
10 N87dc3b912e5b4c068b21abc97de27e92
11 N9b929912503c4e26bc4f820902baa83b
12 Na26856750c0645f39247b1e14a76e7b0
13 Na464570c565540ae9561e3da166171e2
14 Nba191a14d8514833bcffda6bff72acde
15 Ncd0c1e273a424df79d20351816e3c8f7
16 anzsrc-for:06
17 anzsrc-for:0601
18 schema:author N78a00dc4d7674ff89d406b9db49b36ac
19 schema:citation sg:pub.10.1007/s00018-014-1761-6
20 sg:pub.10.1023/b:jsfg.0000029204.57846.7d
21 sg:pub.10.1038/srep30442
22 https://doi.org/10.1002/jcc.20084
23 https://doi.org/10.1016/j.bbamem.2007.10.018
24 https://doi.org/10.1016/j.bbrc.2011.05.161
25 https://doi.org/10.1016/j.bcp.2007.12.016
26 https://doi.org/10.1016/s0092-8674(00)81553-6
27 https://doi.org/10.1016/s0092-8674(01)00200-8
28 https://doi.org/10.1053/j.gastro.2004.07.022
29 https://doi.org/10.1063/1.3463362
30 https://doi.org/10.1073/pnas.181342398
31 https://doi.org/10.1074/jbc.m109.043752
32 https://doi.org/10.1074/jbc.m111.228478
33 https://doi.org/10.1083/jcb.136.6.1239
34 https://doi.org/10.1083/jcb.141.7.1539
35 https://doi.org/10.1083/jcb.147.1.195
36 https://doi.org/10.1083/jcb.200110122
37 https://doi.org/10.1083/jcb.200302070
38 https://doi.org/10.1085/jgp.200810154
39 https://doi.org/10.1086/508617
40 https://doi.org/10.1088/1742-6596/425/1/012002
41 https://doi.org/10.1093/nar/gku340
42 https://doi.org/10.1107/s0021889807021206
43 https://doi.org/10.1107/s0907444909042073
44 https://doi.org/10.1107/s0907444909052925
45 https://doi.org/10.1107/s0907444910007493
46 https://doi.org/10.1107/s0907444998003254
47 https://doi.org/10.1126/science.1248571
48 https://doi.org/10.1126/science.1261833
49 https://doi.org/10.1126/science.285.5424.103
50 https://doi.org/10.1128/mbio.00594-12
51 https://doi.org/10.3390/toxins2061336
52 https://doi.org/10.4161/tisb.25247
53 schema:datePublished 2016-12
54 schema:datePublishedReg 2016-12-01
55 schema:description The food-poisoning bacterium Clostridium perfringens produces an enterotoxin (~35 kDa) that specifically targets human claudin-4, among the 26 human claudin proteins, and causes diarrhea by fluid accumulation in the intestinal cavity. The C-terminal domain of the Clostridium perfringens enterotoxin (C-CPE, ~15 kDa) binds tightly to claudin-4, and disrupts the intestinal tight junction barriers. In this study, we determined the 3.5-Å resolution crystal structure of the cell-free synthesized human claudin-4•C-CPE complex, which is significantly different from the structure of the off-target complex of an engineered C-CPE with mouse claudin-19. The claudin-4•C-CPE complex structure demonstrated the mechanism underlying claudin assembly disruption. A comparison of the present C-CPE-bound structure of claudin-4 with the enterotoxin-free claudin-15 structure revealed sophisticated C-CPE-induced conformation changes of the extracellular segments, induced on the foundation of the rigid four-transmembrane-helix bundle structure. These conformation changes provide a mechanistic model for the disruption of the lateral assembly of claudin molecules. Furthermore, the present novel structural mechanism for selecting a specific member of the claudin family can be used as the foundation to develop novel medically important technologies to selectively regulate the tight junctions formed by claudin family members in different organs.
56 schema:genre research_article
57 schema:inLanguage en
58 schema:isAccessibleForFree true
59 schema:isPartOf N916fcdb9e3424e249421d12e7c3a3e3d
60 Nd296e3a0b0a64ab9ab5f25e732292c60
61 sg:journal.1045337
62 schema:name Structural basis for disruption of claudin assembly in tight junctions by an enterotoxin
63 schema:pagination 33632
64 schema:productId N29fbd21abf8f40d1a9040cc4aec841f3
65 N34d9820a7051466184613dfa9a6b49eb
66 N67960e34414a4add99552cba8bb5d38c
67 Ncd8a8240133f424da571765dac0f28bd
68 Nd81545b5b5e9451b90f5ea420de1d986
69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009437358
70 https://doi.org/10.1038/srep33632
71 schema:sdDatePublished 2019-04-10T19:15
72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
73 schema:sdPublisher Nb5425e55d59649818b3a608c1e220758
74 schema:url http://www.nature.com/srep/2016/160920/srep33632/full/srep33632.html
75 sgo:license sg:explorer/license/
76 sgo:sdDataset articles
77 rdf:type schema:ScholarlyArticle
78 N0fc241e44acf461facac24612254c932 rdf:first sg:person.01274360652.26
79 rdf:rest rdf:nil
80 N10fc1b7b6616437297ddb6da91bfaa7b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Tight Junctions
82 rdf:type schema:DefinedTerm
83 N141b3dc83cf14d6d9f3186d81987ee6e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Mice
85 rdf:type schema:DefinedTerm
86 N1cfc2dbe4d774839b89aded8faa7d6ef schema:name RIKEN SPring-8 Center, 1-1-1, Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
87 rdf:type schema:Organization
88 N2039cc274d524d78aef66f7728f67f8c rdf:first sg:person.01071370106.37
89 rdf:rest N34f8fbbfd11c4e1d8580e5a42239c031
90 N29fbd21abf8f40d1a9040cc4aec841f3 schema:name dimensions_id
91 schema:value pub.1009437358
92 rdf:type schema:PropertyValue
93 N2b727aeb13fc42be8fe53d621ac37efe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Binding Sites
95 rdf:type schema:DefinedTerm
96 N2cd87b6b907342a09be7771de42ed6b7 schema:name RIKEN SPring-8 Center, 1-1-1, Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
97 rdf:type schema:Organization
98 N2dd8eeccc65d4ba0aeaca13145a4121b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Models, Molecular
100 rdf:type schema:DefinedTerm
101 N2efdfed5ca2246e68ac0ddc37d129afd rdf:first sg:person.012771421304.81
102 rdf:rest N7799c7fb46c14472a9765fc9cf5fca76
103 N34d9820a7051466184613dfa9a6b49eb schema:name doi
104 schema:value 10.1038/srep33632
105 rdf:type schema:PropertyValue
106 N34e971cb7d0f43bfb27db569393f0058 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Hydrophobic and Hydrophilic Interactions
108 rdf:type schema:DefinedTerm
109 N34f8fbbfd11c4e1d8580e5a42239c031 rdf:first sg:person.013626202062.24
110 rdf:rest N8e0c2c2ba75642cfa59564c393364d7c
111 N3b44396437ea4c98a3a13533fd9aedf0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Claudins
113 rdf:type schema:DefinedTerm
114 N44b083d4b9fc4139a8082c7666257c4e schema:name RIKEN SPring-8 Center, 1-1-1, Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
115 rdf:type schema:Organization
116 N52f96f0e853746469d72b4006ab9920c rdf:first sg:person.0723444525.34
117 rdf:rest N0fc241e44acf461facac24612254c932
118 N5cfd70adb9a840028077537b7d6f35ce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Models, Biological
120 rdf:type schema:DefinedTerm
121 N67960e34414a4add99552cba8bb5d38c schema:name nlm_unique_id
122 schema:value 101563288
123 rdf:type schema:PropertyValue
124 N6bcd4c212e8d497a98452729ded6504a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Enterotoxins
126 rdf:type schema:DefinedTerm
127 N7799c7fb46c14472a9765fc9cf5fca76 rdf:first sg:person.01104104440.76
128 rdf:rest N52f96f0e853746469d72b4006ab9920c
129 N78a00dc4d7674ff89d406b9db49b36ac rdf:first sg:person.0762157770.83
130 rdf:rest Ne84a05d5a5d54becbd7c287e85e3b68b
131 N8320ae7e4a6146f7abc971aef170d137 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Protein Conformation
133 rdf:type schema:DefinedTerm
134 N87dc3b912e5b4c068b21abc97de27e92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Humans
136 rdf:type schema:DefinedTerm
137 N8e0c2c2ba75642cfa59564c393364d7c rdf:first sg:person.01256470305.03
138 rdf:rest N9a8385b553624bf8858bf63fd469c4a0
139 N916fcdb9e3424e249421d12e7c3a3e3d schema:issueNumber 1
140 rdf:type schema:PublicationIssue
141 N9a8385b553624bf8858bf63fd469c4a0 rdf:first sg:person.0756317004.75
142 rdf:rest N2efdfed5ca2246e68ac0ddc37d129afd
143 N9b929912503c4e26bc4f820902baa83b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Structure-Activity Relationship
145 rdf:type schema:DefinedTerm
146 Na26856750c0645f39247b1e14a76e7b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Mutation
148 rdf:type schema:DefinedTerm
149 Na464570c565540ae9561e3da166171e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Animals
151 rdf:type schema:DefinedTerm
152 Nb5425e55d59649818b3a608c1e220758 schema:name Springer Nature - SN SciGraph project
153 rdf:type schema:Organization
154 Nba191a14d8514833bcffda6bff72acde schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Amino Acid Sequence
156 rdf:type schema:DefinedTerm
157 Nc26ea677ed174a6281d5c3e1dbc7d6cf rdf:first sg:person.01303771126.15
158 rdf:rest N2039cc274d524d78aef66f7728f67f8c
159 Ncd0c1e273a424df79d20351816e3c8f7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Protein Binding
161 rdf:type schema:DefinedTerm
162 Ncd8a8240133f424da571765dac0f28bd schema:name pubmed_id
163 schema:value 27647526
164 rdf:type schema:PropertyValue
165 Nd296e3a0b0a64ab9ab5f25e732292c60 schema:volumeNumber 6
166 rdf:type schema:PublicationVolume
167 Nd81545b5b5e9451b90f5ea420de1d986 schema:name readcube_id
168 schema:value 2cf4e9e1f99737a20066b935313905294cc5639e208612867fec5f918545d379
169 rdf:type schema:PropertyValue
170 Ne84a05d5a5d54becbd7c287e85e3b68b rdf:first sg:person.01117656406.29
171 rdf:rest Nc26ea677ed174a6281d5c3e1dbc7d6cf
172 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
173 schema:name Biological Sciences
174 rdf:type schema:DefinedTerm
175 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
176 schema:name Biochemistry and Cell Biology
177 rdf:type schema:DefinedTerm
178 sg:grant.6129713 http://pending.schema.org/fundedItem sg:pub.10.1038/srep33632
179 rdf:type schema:MonetaryGrant
180 sg:journal.1045337 schema:issn 2045-2322
181 schema:name Scientific Reports
182 rdf:type schema:Periodical
183 sg:person.01071370106.37 schema:affiliation https://www.grid.ac/institutes/grid.7597.c
184 schema:familyName Ohsawa
185 schema:givenName Noboru
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071370106.37
187 rdf:type schema:Person
188 sg:person.01104104440.76 schema:affiliation https://www.grid.ac/institutes/grid.7597.c
189 schema:familyName Kimura-Someya
190 schema:givenName Tomomi
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01104104440.76
192 rdf:type schema:Person
193 sg:person.01117656406.29 schema:affiliation https://www.grid.ac/institutes/grid.7597.c
194 schema:familyName Shinya
195 schema:givenName Naoko
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01117656406.29
197 rdf:type schema:Person
198 sg:person.01256470305.03 schema:affiliation N1cfc2dbe4d774839b89aded8faa7d6ef
199 schema:familyName Hirata
200 schema:givenName Kunio
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01256470305.03
202 rdf:type schema:Person
203 sg:person.01274360652.26 schema:affiliation https://www.grid.ac/institutes/grid.7597.c
204 schema:familyName Shirouzu
205 schema:givenName Mikako
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274360652.26
207 rdf:type schema:Person
208 sg:person.012771421304.81 schema:affiliation N2cd87b6b907342a09be7771de42ed6b7
209 schema:familyName Yamamoto
210 schema:givenName Masaki
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012771421304.81
212 rdf:type schema:Person
213 sg:person.01303771126.15 schema:affiliation https://www.grid.ac/institutes/grid.7597.c
214 schema:familyName Ito
215 schema:givenName Kaori
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303771126.15
217 rdf:type schema:Person
218 sg:person.013626202062.24 schema:affiliation https://www.grid.ac/institutes/grid.7597.c
219 schema:familyName Terada
220 schema:givenName Takaho
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013626202062.24
222 rdf:type schema:Person
223 sg:person.0723444525.34 schema:affiliation https://www.grid.ac/institutes/grid.7597.c
224 schema:familyName Yokoyama
225 schema:givenName Shigeyuki
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0723444525.34
227 rdf:type schema:Person
228 sg:person.0756317004.75 schema:affiliation N44b083d4b9fc4139a8082c7666257c4e
229 schema:familyName Kawano
230 schema:givenName Yoshiaki
231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0756317004.75
232 rdf:type schema:Person
233 sg:person.0762157770.83 schema:affiliation https://www.grid.ac/institutes/grid.7597.c
234 schema:familyName Shinoda
235 schema:givenName Takehiro
236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762157770.83
237 rdf:type schema:Person
238 sg:pub.10.1007/s00018-014-1761-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014596807
239 https://doi.org/10.1007/s00018-014-1761-6
240 rdf:type schema:CreativeWork
241 sg:pub.10.1023/b:jsfg.0000029204.57846.7d schema:sameAs https://app.dimensions.ai/details/publication/pub.1022949189
242 https://doi.org/10.1023/b:jsfg.0000029204.57846.7d
243 rdf:type schema:CreativeWork
244 sg:pub.10.1038/srep30442 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029400865
245 https://doi.org/10.1038/srep30442
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1002/jcc.20084 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008225564
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1016/j.bbamem.2007.10.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033419088
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1016/j.bbrc.2011.05.161 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011189595
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1016/j.bcp.2007.12.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038183210
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1016/s0092-8674(00)81553-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025140525
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1016/s0092-8674(01)00200-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029078331
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1053/j.gastro.2004.07.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036459927
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1063/1.3463362 schema:sameAs https://app.dimensions.ai/details/publication/pub.1098526006
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1073/pnas.181342398 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006228920
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1074/jbc.m109.043752 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046559787
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1074/jbc.m111.228478 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040355978
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1083/jcb.136.6.1239 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027081156
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1083/jcb.141.7.1539 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044261156
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1083/jcb.147.1.195 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048718676
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1083/jcb.200110122 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047702118
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1083/jcb.200302070 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045853086
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1085/jgp.200810154 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022094485
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1086/508617 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058786372
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1088/1742-6596/425/1/012002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044156712
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1093/nar/gku340 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052806377
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1107/s0021889807021206 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012753217
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1107/s0907444909042073 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018458004
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1107/s0907444909052925 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020777046
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1107/s0907444910007493 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000235100
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1107/s0907444998003254 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019779527
296 rdf:type schema:CreativeWork
297 https://doi.org/10.1126/science.1248571 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062469262
298 rdf:type schema:CreativeWork
299 https://doi.org/10.1126/science.1261833 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062470874
300 rdf:type schema:CreativeWork
301 https://doi.org/10.1126/science.285.5424.103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062565757
302 rdf:type schema:CreativeWork
303 https://doi.org/10.1128/mbio.00594-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053721695
304 rdf:type schema:CreativeWork
305 https://doi.org/10.3390/toxins2061336 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015107442
306 rdf:type schema:CreativeWork
307 https://doi.org/10.4161/tisb.25247 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072311074
308 rdf:type schema:CreativeWork
309 https://www.grid.ac/institutes/grid.7597.c schema:alternateName RIKEN
310 schema:name Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
311 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
312 RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
313 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...