Quantitative and combinatory determination of in situ phosphorylation of tau and its FTDP-17 mutants View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-12

AUTHORS

Taeko Kimura, Tomohisa Hosokawa, Masato Taoka, Koji Tsutsumi, Kanae Ando, Koichi Ishiguro, Masato Hosokawa, Masato Hasegawa, Shin-Ichi Hisanaga

ABSTRACT

Tau is hyperphosphorylated in the brains of patients with tauopathies, such as Alzheimer's disease and frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17). However, neither the mechanism of hyperphosphorylation nor its contribution to pathogenesis is known. We applied Phos-tag SDS-PAGE, a phosphoaffinity electrophoresis, to the analysis of tau phosphorylation in vitro by Cdk5, in cultured cells and in mouse brain. Here, we found that Cdk5-p25 phosphorylated tau in vitro at Ser404, Ser235, Thr205 and Ser202 in this order. In contrast in cultured cells, Ser404 was preferentially phosphorylated by Cdk5-p35, whereas Thr205 was not phosphorylated. Ser202 and Ser235 were phosphorylated by endogenous kinases. Tau exhibited ~12 phosphorylation isotypes in COS-7 cells with different combinations of phosphorylation at Thr181, Ser202, Thr231, Ser235 and Ser404. These phosphorylation sites were similar to tau phosphorylated in mouse brains. FTDP-17 tau with a mutation in the C-terminal region had different banding patterns, indicating a different phosphorylation pattern. In particular, it was clear that the R406W mutation causes loss of Ser404 phosphorylation. These results demonstrate the usefulness of the Phos-tag technique in the quantitative analysis of site-specific in vivo phosphorylation of tau and provide detailed information on in situ combinatory phosphorylation of tau. More... »

PAGES

33479

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep33479

DOI

http://dx.doi.org/10.1038/srep33479

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010905409

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27641626


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Neurosciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Alanine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Brain", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "COS Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cercopithecus aethiops", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cyclin-Dependent Kinase 5", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Frontotemporal Dementia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Biological", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphorylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "tau Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Tokyo Metropolitan University", 
          "id": "https://www.grid.ac/institutes/grid.265074.2", 
          "name": [
            "Laboratory of Molecular Neuroscience, Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kimura", 
        "givenName": "Taeko", 
        "id": "sg:person.01351675054.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01351675054.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tokyo Metropolitan University", 
          "id": "https://www.grid.ac/institutes/grid.265074.2", 
          "name": [
            "Laboratory of Molecular Neuroscience, Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hosokawa", 
        "givenName": "Tomohisa", 
        "id": "sg:person.01235562547.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235562547.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tokyo Metropolitan University", 
          "id": "https://www.grid.ac/institutes/grid.265074.2", 
          "name": [
            "Department of Chemistry, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Taoka", 
        "givenName": "Masato", 
        "id": "sg:person.0604703670.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604703670.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tokyo Metropolitan University", 
          "id": "https://www.grid.ac/institutes/grid.265074.2", 
          "name": [
            "Laboratory of Molecular Neuroscience, Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tsutsumi", 
        "givenName": "Koji", 
        "id": "sg:person.0733225101.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0733225101.93"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tokyo Metropolitan University", 
          "id": "https://www.grid.ac/institutes/grid.265074.2", 
          "name": [
            "Laboratory of Molecular Neuroscience, Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ando", 
        "givenName": "Kanae", 
        "id": "sg:person.01041350337.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01041350337.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Juntendo University", 
          "id": "https://www.grid.ac/institutes/grid.258269.2", 
          "name": [
            "Juntendo University, Tokyo, 113-0033, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ishiguro", 
        "givenName": "Koichi", 
        "id": "sg:person.0604005564.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604005564.91"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tokyo Metropolitan Institute of Medical Science", 
          "id": "https://www.grid.ac/institutes/grid.272456.0", 
          "name": [
            "Tokyo Metropolitan Institute of Medical Science, Setagaya, Tokyo 156-8506, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hosokawa", 
        "givenName": "Masato", 
        "id": "sg:person.012763007552.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012763007552.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tokyo Metropolitan Institute of Medical Science", 
          "id": "https://www.grid.ac/institutes/grid.272456.0", 
          "name": [
            "Tokyo Metropolitan Institute of Medical Science, Setagaya, Tokyo 156-8506, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hasegawa", 
        "givenName": "Masato", 
        "id": "sg:person.01155272521.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01155272521.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tokyo Metropolitan University", 
          "id": "https://www.grid.ac/institutes/grid.265074.2", 
          "name": [
            "Laboratory of Molecular Neuroscience, Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hisanaga", 
        "givenName": "Shin-Ichi", 
        "id": "sg:person.0601061521.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601061521.94"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1074/jbc.m311203200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001054866"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m112.433326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003298421"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m504792200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005689319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m504792200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005689319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35012636", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007843170", 
          "https://doi.org/10.1038/35012636"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35012636", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007843170", 
          "https://doi.org/10.1038/35012636"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(98)01217-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007977315"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0002-9440(10)64448-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008686884"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.brainresbull.2010.11.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010291926"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1474-4422(13)70090-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011761558"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.2010.07050.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011962295"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.2010.07050.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011962295"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(93)81066-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013362544"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuropharm.2004.09.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014206937"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jnr.490390607", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014264304"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0002-9440(10)63979-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015154077"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/45159", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015651802", 
          "https://doi.org/10.1038/45159"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/45159", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015651802", 
          "https://doi.org/10.1038/45159"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m405131200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016723000"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.2004.02155.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017399751"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fnmol.2014.00065", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017470178"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1531-8249(199910)46:4<617::aid-ana10>3.0.co;2-i", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017688405"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.270.2.823", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018099614"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2004-6-1-204", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019256706", 
          "https://doi.org/10.1186/gb-2004-6-1-204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbadis.2004.09.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020494720"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.201007161", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021128292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrn.2015.1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021522996", 
          "https://doi.org/10.1038/nrn.2015.1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0306-4522(01)00434-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024111046"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.5927-11.2012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024820593"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0521543", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024978712"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0521543", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024978712"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m511697200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025394766"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m907757199", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025729215"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1630428100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028428842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0197-4580(00)00202-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028807541"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrn2194", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032680057", 
          "https://doi.org/10.1038/nrn2194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ana.410420406", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032890278"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.40.25326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033442523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molmed.2009.01.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034678960"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.6.10.1397", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035387984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/exnr.2002.8014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036224473"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/exnr.2002.8014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036224473"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m603469200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036330376"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ajpath.2015.10.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037564738"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-cellbio-092910-154023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040572995"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.96.20.11156", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041137594"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbadis.2009.05.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041375780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/mcp.m900578-mcp200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043285866"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2007.04.187", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047933970"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/brain/122.4.741", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048045956"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/mcp.t500024-mcp200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048316396"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrn.2015.3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048832183", 
          "https://doi.org/10.1038/nrn.2015.3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.147553", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048941635"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.2007.04505.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050614006"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fnins.2014.00458", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050657871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1373/49.10.1717", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051829735"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1471-4159.2000.0742583.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052133256"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi051635j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055201040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi051635j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055201040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi100631f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055203256"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi100631f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055203256"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.jbchem.a022174", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059960975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci20640", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063416553"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3233/jad-2012-129031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078579926"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082594101", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082719512", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082793947", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083066897", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-12", 
    "datePublishedReg": "2016-12-01", 
    "description": "Tau is hyperphosphorylated in the brains of patients with tauopathies, such as Alzheimer's disease and frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17). However, neither the mechanism of hyperphosphorylation nor its contribution to pathogenesis is known. We applied Phos-tag SDS-PAGE, a phosphoaffinity electrophoresis, to the analysis of tau phosphorylation in vitro by Cdk5, in cultured cells and in mouse brain. Here, we found that Cdk5-p25 phosphorylated tau in vitro at Ser404, Ser235, Thr205 and Ser202 in this order. In contrast in cultured cells, Ser404 was preferentially phosphorylated by Cdk5-p35, whereas Thr205 was not phosphorylated. Ser202 and Ser235 were phosphorylated by endogenous kinases. Tau exhibited ~12 phosphorylation isotypes in COS-7 cells with different combinations of phosphorylation at Thr181, Ser202, Thr231, Ser235 and Ser404. These phosphorylation sites were similar to tau phosphorylated in mouse brains. FTDP-17 tau with a mutation in the C-terminal region had different banding patterns, indicating a different phosphorylation pattern. In particular, it was clear that the R406W mutation causes loss of Ser404 phosphorylation. These results demonstrate the usefulness of the Phos-tag technique in the quantitative analysis of site-specific in vivo phosphorylation of tau and provide detailed information on in situ combinatory phosphorylation of tau.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/srep33479", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.5884407", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6126566", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5909703", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "6"
      }
    ], 
    "name": "Quantitative and combinatory determination of in situ phosphorylation of tau and its FTDP-17 mutants", 
    "pagination": "33479", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "33f4ccadc0a3988fc158924f440ee034841852942b9e8e577599f0139f2333fe"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "27641626"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/srep33479"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1010905409"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/srep33479", 
      "https://app.dimensions.ai/details/publication/pub.1010905409"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T13:29", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8659_00000582.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/srep/2016/160919/srep33479/full/srep33479.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/srep33479'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/srep33479'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/srep33479'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/srep33479'


 

This table displays all metadata directly associated to this object as RDF triples.

375 TRIPLES      21 PREDICATES      103 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/srep33479 schema:about N13f477c27c7c4bf1ab9950ab3350599a
2 N2f05668cd64c4fe7a8adbc7b912d1a7a
3 N2fc70f56f65f456e8038c6ba083cde6a
4 N57da675c50cb4db9ac2ebabb0e838d7c
5 N842c2cf286a447f49d2ef4077543c09c
6 N8abbabe267374735b90a717cb2fe9521
7 Na50ccbd63c3341f6b827135e70966ca1
8 Na9c9cbe4fd594f6d935c68128a589f1d
9 Nb3ae064b49b0426abb8bdae9db96bcc6
10 Nc3c2fe5050cd4a8e8dee1a0367d7d177
11 Nca7f044a492b42168c28cd7c5ca702e5
12 Nd4600f727b144f5e94a48b53728680fa
13 Nd74301de35104a78b9d7c6d707e2ae36
14 Nf293ea4d58054717b0f9c0cad10c0921
15 anzsrc-for:11
16 anzsrc-for:1109
17 schema:author N603317172737454fa854837e0a116ea2
18 schema:citation sg:pub.10.1038/35012636
19 sg:pub.10.1038/45159
20 sg:pub.10.1038/nrn.2015.1
21 sg:pub.10.1038/nrn.2015.3
22 sg:pub.10.1038/nrn2194
23 sg:pub.10.1186/gb-2004-6-1-204
24 https://app.dimensions.ai/details/publication/pub.1082594101
25 https://app.dimensions.ai/details/publication/pub.1082719512
26 https://app.dimensions.ai/details/publication/pub.1082793947
27 https://app.dimensions.ai/details/publication/pub.1083066897
28 https://doi.org/10.1002/1531-8249(199910)46:4<617::aid-ana10>3.0.co;2-i
29 https://doi.org/10.1002/ana.410420406
30 https://doi.org/10.1002/jnr.490390607
31 https://doi.org/10.1006/exnr.2002.8014
32 https://doi.org/10.1016/0014-5793(93)81066-9
33 https://doi.org/10.1016/j.ajpath.2015.10.009
34 https://doi.org/10.1016/j.bbadis.2004.09.008
35 https://doi.org/10.1016/j.bbadis.2009.05.001
36 https://doi.org/10.1016/j.bbrc.2007.04.187
37 https://doi.org/10.1016/j.brainresbull.2010.11.016
38 https://doi.org/10.1016/j.molmed.2009.01.003
39 https://doi.org/10.1016/j.neuropharm.2004.09.014
40 https://doi.org/10.1016/s0002-9440(10)63979-x
41 https://doi.org/10.1016/s0002-9440(10)64448-3
42 https://doi.org/10.1016/s0014-5793(98)01217-4
43 https://doi.org/10.1016/s0197-4580(00)00202-5
44 https://doi.org/10.1016/s0306-4522(01)00434-1
45 https://doi.org/10.1016/s1474-4422(13)70090-5
46 https://doi.org/10.1021/bi051635j
47 https://doi.org/10.1021/bi0521543
48 https://doi.org/10.1021/bi100631f
49 https://doi.org/10.1046/j.1471-4159.2000.0742583.x
50 https://doi.org/10.1073/pnas.1630428100
51 https://doi.org/10.1073/pnas.96.20.11156
52 https://doi.org/10.1074/jbc.270.2.823
53 https://doi.org/10.1074/jbc.272.40.25326
54 https://doi.org/10.1074/jbc.m112.433326
55 https://doi.org/10.1074/jbc.m311203200
56 https://doi.org/10.1074/jbc.m405131200
57 https://doi.org/10.1074/jbc.m504792200
58 https://doi.org/10.1074/jbc.m511697200
59 https://doi.org/10.1074/jbc.m603469200
60 https://doi.org/10.1074/jbc.m907757199
61 https://doi.org/10.1074/mcp.m900578-mcp200
62 https://doi.org/10.1074/mcp.t500024-mcp200
63 https://doi.org/10.1083/jcb.201007161
64 https://doi.org/10.1091/mbc.6.10.1397
65 https://doi.org/10.1093/brain/122.4.741
66 https://doi.org/10.1093/oxfordjournals.jbchem.a022174
67 https://doi.org/10.1111/j.1471-4159.2004.02155.x
68 https://doi.org/10.1111/j.1471-4159.2007.04505.x
69 https://doi.org/10.1111/j.1471-4159.2010.07050.x
70 https://doi.org/10.1146/annurev-cellbio-092910-154023
71 https://doi.org/10.1172/jci20640
72 https://doi.org/10.1242/jcs.147553
73 https://doi.org/10.1373/49.10.1717
74 https://doi.org/10.1523/jneurosci.5927-11.2012
75 https://doi.org/10.3233/jad-2012-129031
76 https://doi.org/10.3389/fnins.2014.00458
77 https://doi.org/10.3389/fnmol.2014.00065
78 schema:datePublished 2016-12
79 schema:datePublishedReg 2016-12-01
80 schema:description Tau is hyperphosphorylated in the brains of patients with tauopathies, such as Alzheimer's disease and frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17). However, neither the mechanism of hyperphosphorylation nor its contribution to pathogenesis is known. We applied Phos-tag SDS-PAGE, a phosphoaffinity electrophoresis, to the analysis of tau phosphorylation in vitro by Cdk5, in cultured cells and in mouse brain. Here, we found that Cdk5-p25 phosphorylated tau in vitro at Ser404, Ser235, Thr205 and Ser202 in this order. In contrast in cultured cells, Ser404 was preferentially phosphorylated by Cdk5-p35, whereas Thr205 was not phosphorylated. Ser202 and Ser235 were phosphorylated by endogenous kinases. Tau exhibited ~12 phosphorylation isotypes in COS-7 cells with different combinations of phosphorylation at Thr181, Ser202, Thr231, Ser235 and Ser404. These phosphorylation sites were similar to tau phosphorylated in mouse brains. FTDP-17 tau with a mutation in the C-terminal region had different banding patterns, indicating a different phosphorylation pattern. In particular, it was clear that the R406W mutation causes loss of Ser404 phosphorylation. These results demonstrate the usefulness of the Phos-tag technique in the quantitative analysis of site-specific in vivo phosphorylation of tau and provide detailed information on in situ combinatory phosphorylation of tau.
81 schema:genre research_article
82 schema:inLanguage en
83 schema:isAccessibleForFree true
84 schema:isPartOf N485a642dbc174fe5aed7f983554b15dc
85 Nd3b15af44f5844e6a9bdf44d58f8d2cc
86 sg:journal.1045337
87 schema:name Quantitative and combinatory determination of in situ phosphorylation of tau and its FTDP-17 mutants
88 schema:pagination 33479
89 schema:productId N2cd22513a1bc439689c47ecfe5cb77a4
90 N579e5c27c7ec48fbab4c500c146ab087
91 N6939876bcfb243b484d8c50b65373b50
92 N892a391cbc7c40ceb0d6f8b604580c93
93 Nd8dd4aad4ced457bb0554a0c882b3237
94 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010905409
95 https://doi.org/10.1038/srep33479
96 schema:sdDatePublished 2019-04-10T13:29
97 schema:sdLicense https://scigraph.springernature.com/explorer/license/
98 schema:sdPublisher N033ffcd471c840338db25876097038b3
99 schema:url http://www.nature.com/srep/2016/160919/srep33479/full/srep33479.html
100 sgo:license sg:explorer/license/
101 sgo:sdDataset articles
102 rdf:type schema:ScholarlyArticle
103 N033ffcd471c840338db25876097038b3 schema:name Springer Nature - SN SciGraph project
104 rdf:type schema:Organization
105 N10e034e045994b1988657413a7071700 rdf:first sg:person.0601061521.94
106 rdf:rest rdf:nil
107 N13f477c27c7c4bf1ab9950ab3350599a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Alanine
109 rdf:type schema:DefinedTerm
110 N2cd22513a1bc439689c47ecfe5cb77a4 schema:name dimensions_id
111 schema:value pub.1010905409
112 rdf:type schema:PropertyValue
113 N2f05668cd64c4fe7a8adbc7b912d1a7a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Mutant Proteins
115 rdf:type schema:DefinedTerm
116 N2fc70f56f65f456e8038c6ba083cde6a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Phosphorylation
118 rdf:type schema:DefinedTerm
119 N485a642dbc174fe5aed7f983554b15dc schema:volumeNumber 6
120 rdf:type schema:PublicationVolume
121 N512d569f6510448b945a3bccb369093b rdf:first sg:person.01155272521.20
122 rdf:rest N10e034e045994b1988657413a7071700
123 N579e5c27c7ec48fbab4c500c146ab087 schema:name nlm_unique_id
124 schema:value 101563288
125 rdf:type schema:PropertyValue
126 N57da675c50cb4db9ac2ebabb0e838d7c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Brain
128 rdf:type schema:DefinedTerm
129 N603317172737454fa854837e0a116ea2 rdf:first sg:person.01351675054.00
130 rdf:rest N9cfc655b72c64eb7ba4ffcf808028844
131 N6939876bcfb243b484d8c50b65373b50 schema:name pubmed_id
132 schema:value 27641626
133 rdf:type schema:PropertyValue
134 N8142cd9631e046f38d5aba96461b6528 rdf:first sg:person.0604005564.91
135 rdf:rest N86dfa22ad94e49f886a17ef5b19514c4
136 N842c2cf286a447f49d2ef4077543c09c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Amino Acid Sequence
138 rdf:type schema:DefinedTerm
139 N86dfa22ad94e49f886a17ef5b19514c4 rdf:first sg:person.012763007552.19
140 rdf:rest N512d569f6510448b945a3bccb369093b
141 N892a391cbc7c40ceb0d6f8b604580c93 schema:name doi
142 schema:value 10.1038/srep33479
143 rdf:type schema:PropertyValue
144 N8abbabe267374735b90a717cb2fe9521 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Animals
146 rdf:type schema:DefinedTerm
147 N9c1f4f1e970444b39e408d7a5ac42268 rdf:first sg:person.01041350337.03
148 rdf:rest N8142cd9631e046f38d5aba96461b6528
149 N9cfc655b72c64eb7ba4ffcf808028844 rdf:first sg:person.01235562547.99
150 rdf:rest Nd7711cfa831e42b880fa21739d100da4
151 Na50ccbd63c3341f6b827135e70966ca1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name tau Proteins
153 rdf:type schema:DefinedTerm
154 Na9c9cbe4fd594f6d935c68128a589f1d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Frontotemporal Dementia
156 rdf:type schema:DefinedTerm
157 Nb3ae064b49b0426abb8bdae9db96bcc6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Mutation
159 rdf:type schema:DefinedTerm
160 Nc3c2fe5050cd4a8e8dee1a0367d7d177 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name COS Cells
162 rdf:type schema:DefinedTerm
163 Nca7f044a492b42168c28cd7c5ca702e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Cyclin-Dependent Kinase 5
165 rdf:type schema:DefinedTerm
166 Nd3b15af44f5844e6a9bdf44d58f8d2cc schema:issueNumber 1
167 rdf:type schema:PublicationIssue
168 Nd4600f727b144f5e94a48b53728680fa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Models, Biological
170 rdf:type schema:DefinedTerm
171 Nd74301de35104a78b9d7c6d707e2ae36 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Cercopithecus aethiops
173 rdf:type schema:DefinedTerm
174 Nd7711cfa831e42b880fa21739d100da4 rdf:first sg:person.0604703670.06
175 rdf:rest Nfd35e892232048a5ac34f9e166580c2c
176 Nd8dd4aad4ced457bb0554a0c882b3237 schema:name readcube_id
177 schema:value 33f4ccadc0a3988fc158924f440ee034841852942b9e8e577599f0139f2333fe
178 rdf:type schema:PropertyValue
179 Nf293ea4d58054717b0f9c0cad10c0921 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
180 schema:name Mice
181 rdf:type schema:DefinedTerm
182 Nfd35e892232048a5ac34f9e166580c2c rdf:first sg:person.0733225101.93
183 rdf:rest N9c1f4f1e970444b39e408d7a5ac42268
184 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
185 schema:name Medical and Health Sciences
186 rdf:type schema:DefinedTerm
187 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
188 schema:name Neurosciences
189 rdf:type schema:DefinedTerm
190 sg:grant.5884407 http://pending.schema.org/fundedItem sg:pub.10.1038/srep33479
191 rdf:type schema:MonetaryGrant
192 sg:grant.5909703 http://pending.schema.org/fundedItem sg:pub.10.1038/srep33479
193 rdf:type schema:MonetaryGrant
194 sg:grant.6126566 http://pending.schema.org/fundedItem sg:pub.10.1038/srep33479
195 rdf:type schema:MonetaryGrant
196 sg:journal.1045337 schema:issn 2045-2322
197 schema:name Scientific Reports
198 rdf:type schema:Periodical
199 sg:person.01041350337.03 schema:affiliation https://www.grid.ac/institutes/grid.265074.2
200 schema:familyName Ando
201 schema:givenName Kanae
202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01041350337.03
203 rdf:type schema:Person
204 sg:person.01155272521.20 schema:affiliation https://www.grid.ac/institutes/grid.272456.0
205 schema:familyName Hasegawa
206 schema:givenName Masato
207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01155272521.20
208 rdf:type schema:Person
209 sg:person.01235562547.99 schema:affiliation https://www.grid.ac/institutes/grid.265074.2
210 schema:familyName Hosokawa
211 schema:givenName Tomohisa
212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235562547.99
213 rdf:type schema:Person
214 sg:person.012763007552.19 schema:affiliation https://www.grid.ac/institutes/grid.272456.0
215 schema:familyName Hosokawa
216 schema:givenName Masato
217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012763007552.19
218 rdf:type schema:Person
219 sg:person.01351675054.00 schema:affiliation https://www.grid.ac/institutes/grid.265074.2
220 schema:familyName Kimura
221 schema:givenName Taeko
222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01351675054.00
223 rdf:type schema:Person
224 sg:person.0601061521.94 schema:affiliation https://www.grid.ac/institutes/grid.265074.2
225 schema:familyName Hisanaga
226 schema:givenName Shin-Ichi
227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601061521.94
228 rdf:type schema:Person
229 sg:person.0604005564.91 schema:affiliation https://www.grid.ac/institutes/grid.258269.2
230 schema:familyName Ishiguro
231 schema:givenName Koichi
232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604005564.91
233 rdf:type schema:Person
234 sg:person.0604703670.06 schema:affiliation https://www.grid.ac/institutes/grid.265074.2
235 schema:familyName Taoka
236 schema:givenName Masato
237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604703670.06
238 rdf:type schema:Person
239 sg:person.0733225101.93 schema:affiliation https://www.grid.ac/institutes/grid.265074.2
240 schema:familyName Tsutsumi
241 schema:givenName Koji
242 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0733225101.93
243 rdf:type schema:Person
244 sg:pub.10.1038/35012636 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007843170
245 https://doi.org/10.1038/35012636
246 rdf:type schema:CreativeWork
247 sg:pub.10.1038/45159 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015651802
248 https://doi.org/10.1038/45159
249 rdf:type schema:CreativeWork
250 sg:pub.10.1038/nrn.2015.1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021522996
251 https://doi.org/10.1038/nrn.2015.1
252 rdf:type schema:CreativeWork
253 sg:pub.10.1038/nrn.2015.3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048832183
254 https://doi.org/10.1038/nrn.2015.3
255 rdf:type schema:CreativeWork
256 sg:pub.10.1038/nrn2194 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032680057
257 https://doi.org/10.1038/nrn2194
258 rdf:type schema:CreativeWork
259 sg:pub.10.1186/gb-2004-6-1-204 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019256706
260 https://doi.org/10.1186/gb-2004-6-1-204
261 rdf:type schema:CreativeWork
262 https://app.dimensions.ai/details/publication/pub.1082594101 schema:CreativeWork
263 https://app.dimensions.ai/details/publication/pub.1082719512 schema:CreativeWork
264 https://app.dimensions.ai/details/publication/pub.1082793947 schema:CreativeWork
265 https://app.dimensions.ai/details/publication/pub.1083066897 schema:CreativeWork
266 https://doi.org/10.1002/1531-8249(199910)46:4<617::aid-ana10>3.0.co;2-i schema:sameAs https://app.dimensions.ai/details/publication/pub.1017688405
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1002/ana.410420406 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032890278
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1002/jnr.490390607 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014264304
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1006/exnr.2002.8014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036224473
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1016/0014-5793(93)81066-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013362544
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1016/j.ajpath.2015.10.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037564738
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1016/j.bbadis.2004.09.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020494720
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1016/j.bbadis.2009.05.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041375780
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1016/j.bbrc.2007.04.187 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047933970
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1016/j.brainresbull.2010.11.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010291926
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1016/j.molmed.2009.01.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034678960
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1016/j.neuropharm.2004.09.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014206937
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1016/s0002-9440(10)63979-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1015154077
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1016/s0002-9440(10)64448-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008686884
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1016/s0014-5793(98)01217-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007977315
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1016/s0197-4580(00)00202-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028807541
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1016/s0306-4522(01)00434-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024111046
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1016/s1474-4422(13)70090-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011761558
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1021/bi051635j schema:sameAs https://app.dimensions.ai/details/publication/pub.1055201040
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1021/bi0521543 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024978712
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1021/bi100631f schema:sameAs https://app.dimensions.ai/details/publication/pub.1055203256
307 rdf:type schema:CreativeWork
308 https://doi.org/10.1046/j.1471-4159.2000.0742583.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1052133256
309 rdf:type schema:CreativeWork
310 https://doi.org/10.1073/pnas.1630428100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028428842
311 rdf:type schema:CreativeWork
312 https://doi.org/10.1073/pnas.96.20.11156 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041137594
313 rdf:type schema:CreativeWork
314 https://doi.org/10.1074/jbc.270.2.823 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018099614
315 rdf:type schema:CreativeWork
316 https://doi.org/10.1074/jbc.272.40.25326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033442523
317 rdf:type schema:CreativeWork
318 https://doi.org/10.1074/jbc.m112.433326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003298421
319 rdf:type schema:CreativeWork
320 https://doi.org/10.1074/jbc.m311203200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001054866
321 rdf:type schema:CreativeWork
322 https://doi.org/10.1074/jbc.m405131200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016723000
323 rdf:type schema:CreativeWork
324 https://doi.org/10.1074/jbc.m504792200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005689319
325 rdf:type schema:CreativeWork
326 https://doi.org/10.1074/jbc.m511697200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025394766
327 rdf:type schema:CreativeWork
328 https://doi.org/10.1074/jbc.m603469200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036330376
329 rdf:type schema:CreativeWork
330 https://doi.org/10.1074/jbc.m907757199 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025729215
331 rdf:type schema:CreativeWork
332 https://doi.org/10.1074/mcp.m900578-mcp200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043285866
333 rdf:type schema:CreativeWork
334 https://doi.org/10.1074/mcp.t500024-mcp200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048316396
335 rdf:type schema:CreativeWork
336 https://doi.org/10.1083/jcb.201007161 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021128292
337 rdf:type schema:CreativeWork
338 https://doi.org/10.1091/mbc.6.10.1397 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035387984
339 rdf:type schema:CreativeWork
340 https://doi.org/10.1093/brain/122.4.741 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048045956
341 rdf:type schema:CreativeWork
342 https://doi.org/10.1093/oxfordjournals.jbchem.a022174 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059960975
343 rdf:type schema:CreativeWork
344 https://doi.org/10.1111/j.1471-4159.2004.02155.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017399751
345 rdf:type schema:CreativeWork
346 https://doi.org/10.1111/j.1471-4159.2007.04505.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1050614006
347 rdf:type schema:CreativeWork
348 https://doi.org/10.1111/j.1471-4159.2010.07050.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1011962295
349 rdf:type schema:CreativeWork
350 https://doi.org/10.1146/annurev-cellbio-092910-154023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040572995
351 rdf:type schema:CreativeWork
352 https://doi.org/10.1172/jci20640 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063416553
353 rdf:type schema:CreativeWork
354 https://doi.org/10.1242/jcs.147553 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048941635
355 rdf:type schema:CreativeWork
356 https://doi.org/10.1373/49.10.1717 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051829735
357 rdf:type schema:CreativeWork
358 https://doi.org/10.1523/jneurosci.5927-11.2012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024820593
359 rdf:type schema:CreativeWork
360 https://doi.org/10.3233/jad-2012-129031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078579926
361 rdf:type schema:CreativeWork
362 https://doi.org/10.3389/fnins.2014.00458 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050657871
363 rdf:type schema:CreativeWork
364 https://doi.org/10.3389/fnmol.2014.00065 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017470178
365 rdf:type schema:CreativeWork
366 https://www.grid.ac/institutes/grid.258269.2 schema:alternateName Juntendo University
367 schema:name Juntendo University, Tokyo, 113-0033, Japan.
368 rdf:type schema:Organization
369 https://www.grid.ac/institutes/grid.265074.2 schema:alternateName Tokyo Metropolitan University
370 schema:name Department of Chemistry, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan.
371 Laboratory of Molecular Neuroscience, Department of Biological Sciences, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan.
372 rdf:type schema:Organization
373 https://www.grid.ac/institutes/grid.272456.0 schema:alternateName Tokyo Metropolitan Institute of Medical Science
374 schema:name Tokyo Metropolitan Institute of Medical Science, Setagaya, Tokyo 156-8506, Japan.
375 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...