Genetic characterization of cysteine-rich type-b avenin-like protein coding genes in common wheat View Full Text


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Article Info

DATE

2016-08-09

AUTHORS

X. Y. Chen, X. Y. Cao, Y. J. Zhang, S. Islam, J. J. Zhang, R. C. Yang, J. J. Liu, G. Y. Li, R. Appels, G. Keeble-Gagnere, W. Q. Ji, Z. H. He, W. J. Ma

ABSTRACT

The wheat avenin-like proteins (ALP) are considered atypical gluten constituents and have shown positive effects on dough properties revealed using a transgenic approach. However, to date the genetic architecture of ALP genes is unclear, making it impossible to be utilized in wheat breeding. In the current study, three genes of type-b ALPs were identified and mapped to chromosomes 7AS, 4AL and 7DS. The coding gene sequence of both TaALP-7A and TaALP-7D was 855 bp long, encoding two identical homologous 284 amino acid long proteins. TaALP-4A was 858 bp long, encoding a 285 amino acid protein variant. Three alleles were identified for TaALP-7A and four for TaALP-4A. TaALP-7A alleles were of two types: type-1, which includes TaALP-7A1 andTaALP-7A2, encodes mature proteins, while type-2, represented byTaALP-7A3, contains a stop codon in the coding region and thus does not encode a mature protein. Dough quality testing of 102 wheat cultivars established a highly significant association of the type-1 TaALP-7A allele with better wheat processing quality. This allelic effects were confirmed among a range of commercial wheat cultivars. Our research makes the ALP be the first of such genetic variation source that can be readily utilized in wheat breeding. More... »

PAGES

30692

References to SciGraph publications

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    http://scigraph.springernature.com/pub.10.1038/srep30692

    DOI

    http://dx.doi.org/10.1038/srep30692

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1003756132

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27503660


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