Visualization of Neuregulin 1 ectodomain shedding reveals its local processing in vitro and in vivo View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-09

AUTHORS

Aosa Kamezaki, Fuminori Sato, Kazuhiro Aoki, Kazuhide Asakawa, Koichi Kawakami, Fumio Matsuzaki, Atsuko Sehara-Fujisawa

ABSTRACT

Neuregulin1 (NRG1) plays diverse developmental roles and is likely involved in several neurological disorders including schizophrenia. The transmembrane NRG1 protein is proteolytically cleaved and released as a soluble ligand for ErbB receptors. Such post-translational processing, referred to as 'ectodomain shedding', is thought to be crucial for NRG1 function. However, little is known regarding the regulatory mechanism of NRG1 cleavage in vivo. Here, we developed a fluorescent probe, NRG1 Cleavage Indicating SenSOR (N-CISSOR), by fusing mCherry and GFP to the extracellular and intracellular domains of NRG1, respectively. N-CISSOR mimicked the subcellular localization and biochemical properties of NRG1 including cleavage dynamics and ErbB phosphorylation in cultured cells. mCherry/GFP ratio imaging of phorbol-12-myristate-13-acetate-stimulated N-CISSOR-expressing HEK293T cells enabled to monitor rapid ectodomain shedding of NRG1 at the subcellular level. Utilizing N-CISSOR in zebrafish embryos revealed preferential axonal NRG1 ectodomain shedding in developing motor neurons, demonstrating that NRG1 ectodomain shedding is spatially regulated at the subcellular level. Thus, N-CISSOR will be a valuable tool for elucidating the spatiotemporal regulation of NRG1 ectodomain shedding, both in vitro and in vivo. More... »

PAGES

28873

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep28873

DOI

http://dx.doi.org/10.1038/srep28873

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045039393

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27364328


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals, Genetically Modified", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryo, Nonmammalian", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Green Fluorescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HEK293 Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Luminescent Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Fluorescence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neuregulin-1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phorbol Esters", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Processing, Post-Translational", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteolysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Time-Lapse Imaging", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Zebrafish", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Department of Animal Development and Physiology, Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kamezaki", 
        "givenName": "Aosa", 
        "id": "sg:person.01102076224.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102076224.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Department of Growth Regulation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sato", 
        "givenName": "Fuminori", 
        "id": "sg:person.01155276151.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01155276151.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Imaging Platform for Spatio-Temporal Information, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Aoki", 
        "givenName": "Kazuhiro", 
        "id": "sg:person.01342174731.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342174731.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Genetics", 
          "id": "https://www.grid.ac/institutes/grid.288127.6", 
          "name": [
            "Division of Molecular and Developmental Biology, National Institute of Genetics, and Department of Genetics, SOKENDAI, Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Asakawa", 
        "givenName": "Kazuhide", 
        "id": "sg:person.0727330114.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727330114.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Genetics", 
          "id": "https://www.grid.ac/institutes/grid.288127.6", 
          "name": [
            "Division of Molecular and Developmental Biology, National Institute of Genetics, and Department of Genetics, SOKENDAI, Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kawakami", 
        "givenName": "Koichi", 
        "id": "sg:person.01030362062.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030362062.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "RIKEN Center for Developmental Biology", 
          "id": "https://www.grid.ac/institutes/grid.474692.a", 
          "name": [
            "Laboratory of Cell Asymmetry, RIKEN Center of Developmental Biology, Kobe 650-0047, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matsuzaki", 
        "givenName": "Fumio", 
        "id": "sg:person.013344041721.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013344041721.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Department of Growth Regulation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sehara-Fujisawa", 
        "givenName": "Atsuko", 
        "id": "sg:person.0760217243.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760217243.47"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1096/fj.08-129072", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000067088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1104030108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000636511"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.08-113852", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001569864"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200212085", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005349501"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200310024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005561323"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m007913200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010343476"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/mcne.1996.0019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010406883"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.024802", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010667842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.069997", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013101237"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.1692-04.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013217337"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrn2392", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014121366", 
          "https://doi.org/10.1038/nrn2392"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m109.002915", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015451045"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamcr.2006.11.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015948409"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.00500-15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016145410"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0076853", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018375583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0076853", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018375583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0076853", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018375583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200307137", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018718593"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1548", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019859884", 
          "https://doi.org/10.1038/nrm1548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1548", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019859884", 
          "https://doi.org/10.1038/nrm1548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.3372-12.2013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021033622"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.2004.02719.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021793232"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.11.9.2999", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024254257"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1440-169x.2008.01044.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024798046"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/mcne.1998.0676", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025825814"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m111.251538", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027913388"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1097-2765(00)80416-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033101991"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3630211", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033825333"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3630211", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033825333"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.1659-11.2011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034085318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-4827(02)00102-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034409221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-4827(02)00102-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034409221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0036431", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034941595"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/emboj.2013.146", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035742170"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0704963105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040413964"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nn1797", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040504515", 
          "https://doi.org/10.1038/nn1797"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nn1797", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040504515", 
          "https://doi.org/10.1038/nn1797"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7554/elife.05871", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042974041"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m709872200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044381389"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1307478110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046597739"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1247/csf.13018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046711999"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1000887108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047556443"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/glia.20330", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049400520"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/glia.20330", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049400520"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.00443-10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049941322"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/0264-6021:3630211", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056716094"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jb/mvt030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059746587"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1132341", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062454712"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/scisignal.2005680", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062683757"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074863981", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-09", 
    "datePublishedReg": "2016-09-01", 
    "description": "Neuregulin1 (NRG1) plays diverse developmental roles and is likely involved in several neurological disorders including schizophrenia. The transmembrane NRG1 protein is proteolytically cleaved and released as a soluble ligand for ErbB receptors. Such post-translational processing, referred to as 'ectodomain shedding', is thought to be crucial for NRG1 function. However, little is known regarding the regulatory mechanism of NRG1 cleavage in vivo. Here, we developed a fluorescent probe, NRG1 Cleavage Indicating SenSOR (N-CISSOR), by fusing mCherry and GFP to the extracellular and intracellular domains of NRG1, respectively. N-CISSOR mimicked the subcellular localization and biochemical properties of NRG1 including cleavage dynamics and ErbB phosphorylation in cultured cells. mCherry/GFP ratio imaging of phorbol-12-myristate-13-acetate-stimulated N-CISSOR-expressing HEK293T cells enabled to monitor rapid ectodomain shedding of NRG1 at the subcellular level. Utilizing N-CISSOR in zebrafish embryos revealed preferential axonal NRG1 ectodomain shedding in developing motor neurons, demonstrating that NRG1 ectodomain shedding is spatially regulated at the subcellular level. Thus, N-CISSOR will be a valuable tool for elucidating the spatiotemporal regulation of NRG1 ectodomain shedding, both in vitro and in vivo.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/srep28873", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.5914328", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5827203", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5855044", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6036305", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5871380", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6140372", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6155475", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5903485", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6536528", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.5895942", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "6"
      }
    ], 
    "name": "Visualization of Neuregulin 1 ectodomain shedding reveals its local processing in vitro and in vivo", 
    "pagination": "28873", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d68978332221250c15a00aeaf82e763709244f42927bf2e6144dfcfa0b36afd5"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "27364328"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/srep28873"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1045039393"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/srep28873", 
      "https://app.dimensions.ai/details/publication/pub.1045039393"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:53", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8684_00000551.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/srep/2016/160701/srep28873/full/srep28873.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/srep28873'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/srep28873'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/srep28873'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/srep28873'


 

This table displays all metadata directly associated to this object as RDF triples.

337 TRIPLES      21 PREDICATES      89 URIs      38 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/srep28873 schema:about N001006502e72460ea555e22fc739c387
2 N09f8f693708449d3a99e631017b0dcdd
3 N0d8a8e352c564403a706501d8e5e3a8a
4 N1ac0f5bd50814ef4ba53a7084ab8902a
5 N30cad30df4f748f59663a96a74ea8fba
6 N4d0be28996184f7288067c307523899e
7 N7018e8af2c1c409699985b5ba512ffd2
8 N88de5d2066984c9db26203b65d49b95c
9 N8b1924443325406eb83deac9a49b999c
10 N8d3ec6e59fc444ea817536ba16205462
11 Na65fa11fca6342958244ab162b987bdf
12 Na89e5c86740344929f94b17ab0cda5a3
13 Ndb7b4ba4486c4aa087373a1b9f4d30c5
14 Ndceae77c4b3441368d9878d12742eccc
15 Ne022b7eaa16a4a7ba985ffb6c5f9cf57
16 Nfc77c4596827474c813a3e8f4f6fadd0
17 Nffebaf230b2b4197b9a93be44878bac9
18 anzsrc-for:06
19 anzsrc-for:0601
20 schema:author Nd8d71e026e064d8eada3464697c3ddae
21 schema:citation sg:pub.10.1038/nn1797
22 sg:pub.10.1038/nrm1548
23 sg:pub.10.1038/nrn2392
24 https://app.dimensions.ai/details/publication/pub.1074863981
25 https://doi.org/10.1002/glia.20330
26 https://doi.org/10.1006/mcne.1996.0019
27 https://doi.org/10.1006/mcne.1998.0676
28 https://doi.org/10.1016/j.bbamcr.2006.11.002
29 https://doi.org/10.1016/s0014-4827(02)00102-7
30 https://doi.org/10.1016/s1097-2765(00)80416-5
31 https://doi.org/10.1038/emboj.2013.146
32 https://doi.org/10.1042/0264-6021:3630211
33 https://doi.org/10.1042/bj3630211
34 https://doi.org/10.1073/pnas.0704963105
35 https://doi.org/10.1073/pnas.1000887108
36 https://doi.org/10.1073/pnas.1104030108
37 https://doi.org/10.1073/pnas.1307478110
38 https://doi.org/10.1074/jbc.m007913200
39 https://doi.org/10.1074/jbc.m109.002915
40 https://doi.org/10.1074/jbc.m111.251538
41 https://doi.org/10.1074/jbc.m709872200
42 https://doi.org/10.1083/jcb.200212085
43 https://doi.org/10.1083/jcb.200307137
44 https://doi.org/10.1083/jcb.200310024
45 https://doi.org/10.1091/mbc.11.9.2999
46 https://doi.org/10.1093/jb/mvt030
47 https://doi.org/10.1096/fj.08-113852
48 https://doi.org/10.1096/fj.08-129072
49 https://doi.org/10.1111/j.1440-169x.2008.01044.x
50 https://doi.org/10.1111/j.1471-4159.2004.02719.x
51 https://doi.org/10.1126/science.1132341
52 https://doi.org/10.1126/scisignal.2005680
53 https://doi.org/10.1128/mcb.00443-10
54 https://doi.org/10.1128/mcb.00500-15
55 https://doi.org/10.1242/dev.024802
56 https://doi.org/10.1242/jcs.069997
57 https://doi.org/10.1247/csf.13018
58 https://doi.org/10.1371/journal.pone.0036431
59 https://doi.org/10.1371/journal.pone.0076853
60 https://doi.org/10.1523/jneurosci.1659-11.2011
61 https://doi.org/10.1523/jneurosci.1692-04.2004
62 https://doi.org/10.1523/jneurosci.3372-12.2013
63 https://doi.org/10.7554/elife.05871
64 schema:datePublished 2016-09
65 schema:datePublishedReg 2016-09-01
66 schema:description Neuregulin1 (NRG1) plays diverse developmental roles and is likely involved in several neurological disorders including schizophrenia. The transmembrane NRG1 protein is proteolytically cleaved and released as a soluble ligand for ErbB receptors. Such post-translational processing, referred to as 'ectodomain shedding', is thought to be crucial for NRG1 function. However, little is known regarding the regulatory mechanism of NRG1 cleavage in vivo. Here, we developed a fluorescent probe, NRG1 Cleavage Indicating SenSOR (N-CISSOR), by fusing mCherry and GFP to the extracellular and intracellular domains of NRG1, respectively. N-CISSOR mimicked the subcellular localization and biochemical properties of NRG1 including cleavage dynamics and ErbB phosphorylation in cultured cells. mCherry/GFP ratio imaging of phorbol-12-myristate-13-acetate-stimulated N-CISSOR-expressing HEK293T cells enabled to monitor rapid ectodomain shedding of NRG1 at the subcellular level. Utilizing N-CISSOR in zebrafish embryos revealed preferential axonal NRG1 ectodomain shedding in developing motor neurons, demonstrating that NRG1 ectodomain shedding is spatially regulated at the subcellular level. Thus, N-CISSOR will be a valuable tool for elucidating the spatiotemporal regulation of NRG1 ectodomain shedding, both in vitro and in vivo.
67 schema:genre research_article
68 schema:inLanguage en
69 schema:isAccessibleForFree true
70 schema:isPartOf N413b411013a44ebd8d1755c9d3286e38
71 Nc0caaf08b4054a7aa4fbe9f419876c04
72 sg:journal.1045337
73 schema:name Visualization of Neuregulin 1 ectodomain shedding reveals its local processing in vitro and in vivo
74 schema:pagination 28873
75 schema:productId N0c53dc2d0ca340e380759d1752d88cf3
76 N28a20172142e4e279a1436a7a4681317
77 N784cd0c429c94fdfa0fd5bf93a1dba7c
78 Nb4b5454e788d48ff9e1063b14baa3be1
79 Ncd2869b89cd540599e1ab0114f37407e
80 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045039393
81 https://doi.org/10.1038/srep28873
82 schema:sdDatePublished 2019-04-10T20:53
83 schema:sdLicense https://scigraph.springernature.com/explorer/license/
84 schema:sdPublisher N5207b522434648e5ba07a1575ce0a39f
85 schema:url http://www.nature.com/srep/2016/160701/srep28873/full/srep28873.html
86 sgo:license sg:explorer/license/
87 sgo:sdDataset articles
88 rdf:type schema:ScholarlyArticle
89 N001006502e72460ea555e22fc739c387 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Phorbol Esters
91 rdf:type schema:DefinedTerm
92 N01dc2364b49c4fc885f5d01bec46690d rdf:first sg:person.01155276151.24
93 rdf:rest N5fc9455b8c6048ab8a646e029acb701d
94 N09f8f693708449d3a99e631017b0dcdd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Embryo, Nonmammalian
96 rdf:type schema:DefinedTerm
97 N0c53dc2d0ca340e380759d1752d88cf3 schema:name nlm_unique_id
98 schema:value 101563288
99 rdf:type schema:PropertyValue
100 N0d8a8e352c564403a706501d8e5e3a8a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Time-Lapse Imaging
102 rdf:type schema:DefinedTerm
103 N1ac0f5bd50814ef4ba53a7084ab8902a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Binding Sites
105 rdf:type schema:DefinedTerm
106 N28a20172142e4e279a1436a7a4681317 schema:name readcube_id
107 schema:value d68978332221250c15a00aeaf82e763709244f42927bf2e6144dfcfa0b36afd5
108 rdf:type schema:PropertyValue
109 N30cad30df4f748f59663a96a74ea8fba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Proteolysis
111 rdf:type schema:DefinedTerm
112 N3bb0f545ae9e445d88973273c00b77d5 rdf:first sg:person.0760217243.47
113 rdf:rest rdf:nil
114 N413b411013a44ebd8d1755c9d3286e38 schema:volumeNumber 6
115 rdf:type schema:PublicationVolume
116 N4d0be28996184f7288067c307523899e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Luminescent Proteins
118 rdf:type schema:DefinedTerm
119 N5207b522434648e5ba07a1575ce0a39f schema:name Springer Nature - SN SciGraph project
120 rdf:type schema:Organization
121 N5fc9455b8c6048ab8a646e029acb701d rdf:first sg:person.01342174731.24
122 rdf:rest Nb0e0b45b80f940e3a510a1c8c3dfbedd
123 N7018e8af2c1c409699985b5ba512ffd2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Zebrafish
125 rdf:type schema:DefinedTerm
126 N784cd0c429c94fdfa0fd5bf93a1dba7c schema:name dimensions_id
127 schema:value pub.1045039393
128 rdf:type schema:PropertyValue
129 N88de5d2066984c9db26203b65d49b95c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Microscopy, Fluorescence
131 rdf:type schema:DefinedTerm
132 N8b1924443325406eb83deac9a49b999c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Protein Processing, Post-Translational
134 rdf:type schema:DefinedTerm
135 N8d3ec6e59fc444ea817536ba16205462 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Green Fluorescent Proteins
137 rdf:type schema:DefinedTerm
138 N935c47a813f64bfcbf266171b7c93bc1 rdf:first sg:person.013344041721.29
139 rdf:rest N3bb0f545ae9e445d88973273c00b77d5
140 Na65fa11fca6342958244ab162b987bdf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Neuregulin-1
142 rdf:type schema:DefinedTerm
143 Na89e5c86740344929f94b17ab0cda5a3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Animals, Genetically Modified
145 rdf:type schema:DefinedTerm
146 Nb0e0b45b80f940e3a510a1c8c3dfbedd rdf:first sg:person.0727330114.39
147 rdf:rest Nf79496ad225a4211b95959b90408c93e
148 Nb4b5454e788d48ff9e1063b14baa3be1 schema:name pubmed_id
149 schema:value 27364328
150 rdf:type schema:PropertyValue
151 Nc0caaf08b4054a7aa4fbe9f419876c04 schema:issueNumber 1
152 rdf:type schema:PublicationIssue
153 Ncd2869b89cd540599e1ab0114f37407e schema:name doi
154 schema:value 10.1038/srep28873
155 rdf:type schema:PropertyValue
156 Nd8d71e026e064d8eada3464697c3ddae rdf:first sg:person.01102076224.23
157 rdf:rest N01dc2364b49c4fc885f5d01bec46690d
158 Ndb7b4ba4486c4aa087373a1b9f4d30c5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Animals
160 rdf:type schema:DefinedTerm
161 Ndceae77c4b3441368d9878d12742eccc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name HEK293 Cells
163 rdf:type schema:DefinedTerm
164 Ne022b7eaa16a4a7ba985ffb6c5f9cf57 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Cell Line
166 rdf:type schema:DefinedTerm
167 Nf79496ad225a4211b95959b90408c93e rdf:first sg:person.01030362062.32
168 rdf:rest N935c47a813f64bfcbf266171b7c93bc1
169 Nfc77c4596827474c813a3e8f4f6fadd0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Mice
171 rdf:type schema:DefinedTerm
172 Nffebaf230b2b4197b9a93be44878bac9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Humans
174 rdf:type schema:DefinedTerm
175 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
176 schema:name Biological Sciences
177 rdf:type schema:DefinedTerm
178 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
179 schema:name Biochemistry and Cell Biology
180 rdf:type schema:DefinedTerm
181 sg:grant.5827203 http://pending.schema.org/fundedItem sg:pub.10.1038/srep28873
182 rdf:type schema:MonetaryGrant
183 sg:grant.5855044 http://pending.schema.org/fundedItem sg:pub.10.1038/srep28873
184 rdf:type schema:MonetaryGrant
185 sg:grant.5871380 http://pending.schema.org/fundedItem sg:pub.10.1038/srep28873
186 rdf:type schema:MonetaryGrant
187 sg:grant.5895942 http://pending.schema.org/fundedItem sg:pub.10.1038/srep28873
188 rdf:type schema:MonetaryGrant
189 sg:grant.5903485 http://pending.schema.org/fundedItem sg:pub.10.1038/srep28873
190 rdf:type schema:MonetaryGrant
191 sg:grant.5914328 http://pending.schema.org/fundedItem sg:pub.10.1038/srep28873
192 rdf:type schema:MonetaryGrant
193 sg:grant.6036305 http://pending.schema.org/fundedItem sg:pub.10.1038/srep28873
194 rdf:type schema:MonetaryGrant
195 sg:grant.6140372 http://pending.schema.org/fundedItem sg:pub.10.1038/srep28873
196 rdf:type schema:MonetaryGrant
197 sg:grant.6155475 http://pending.schema.org/fundedItem sg:pub.10.1038/srep28873
198 rdf:type schema:MonetaryGrant
199 sg:grant.6536528 http://pending.schema.org/fundedItem sg:pub.10.1038/srep28873
200 rdf:type schema:MonetaryGrant
201 sg:journal.1045337 schema:issn 2045-2322
202 schema:name Scientific Reports
203 rdf:type schema:Periodical
204 sg:person.01030362062.32 schema:affiliation https://www.grid.ac/institutes/grid.288127.6
205 schema:familyName Kawakami
206 schema:givenName Koichi
207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030362062.32
208 rdf:type schema:Person
209 sg:person.01102076224.23 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
210 schema:familyName Kamezaki
211 schema:givenName Aosa
212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102076224.23
213 rdf:type schema:Person
214 sg:person.01155276151.24 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
215 schema:familyName Sato
216 schema:givenName Fuminori
217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01155276151.24
218 rdf:type schema:Person
219 sg:person.013344041721.29 schema:affiliation https://www.grid.ac/institutes/grid.474692.a
220 schema:familyName Matsuzaki
221 schema:givenName Fumio
222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013344041721.29
223 rdf:type schema:Person
224 sg:person.01342174731.24 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
225 schema:familyName Aoki
226 schema:givenName Kazuhiro
227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342174731.24
228 rdf:type schema:Person
229 sg:person.0727330114.39 schema:affiliation https://www.grid.ac/institutes/grid.288127.6
230 schema:familyName Asakawa
231 schema:givenName Kazuhide
232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727330114.39
233 rdf:type schema:Person
234 sg:person.0760217243.47 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
235 schema:familyName Sehara-Fujisawa
236 schema:givenName Atsuko
237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760217243.47
238 rdf:type schema:Person
239 sg:pub.10.1038/nn1797 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040504515
240 https://doi.org/10.1038/nn1797
241 rdf:type schema:CreativeWork
242 sg:pub.10.1038/nrm1548 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019859884
243 https://doi.org/10.1038/nrm1548
244 rdf:type schema:CreativeWork
245 sg:pub.10.1038/nrn2392 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014121366
246 https://doi.org/10.1038/nrn2392
247 rdf:type schema:CreativeWork
248 https://app.dimensions.ai/details/publication/pub.1074863981 schema:CreativeWork
249 https://doi.org/10.1002/glia.20330 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049400520
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1006/mcne.1996.0019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010406883
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1006/mcne.1998.0676 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025825814
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1016/j.bbamcr.2006.11.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015948409
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1016/s0014-4827(02)00102-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034409221
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1016/s1097-2765(00)80416-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033101991
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1038/emboj.2013.146 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035742170
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1042/0264-6021:3630211 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056716094
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1042/bj3630211 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033825333
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1073/pnas.0704963105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040413964
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1073/pnas.1000887108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047556443
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1073/pnas.1104030108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000636511
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1073/pnas.1307478110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046597739
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1074/jbc.m007913200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010343476
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1074/jbc.m109.002915 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015451045
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1074/jbc.m111.251538 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027913388
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1074/jbc.m709872200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044381389
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1083/jcb.200212085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005349501
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1083/jcb.200307137 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018718593
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1083/jcb.200310024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005561323
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1091/mbc.11.9.2999 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024254257
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1093/jb/mvt030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059746587
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1096/fj.08-113852 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001569864
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1096/fj.08-129072 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000067088
296 rdf:type schema:CreativeWork
297 https://doi.org/10.1111/j.1440-169x.2008.01044.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024798046
298 rdf:type schema:CreativeWork
299 https://doi.org/10.1111/j.1471-4159.2004.02719.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1021793232
300 rdf:type schema:CreativeWork
301 https://doi.org/10.1126/science.1132341 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062454712
302 rdf:type schema:CreativeWork
303 https://doi.org/10.1126/scisignal.2005680 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062683757
304 rdf:type schema:CreativeWork
305 https://doi.org/10.1128/mcb.00443-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049941322
306 rdf:type schema:CreativeWork
307 https://doi.org/10.1128/mcb.00500-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016145410
308 rdf:type schema:CreativeWork
309 https://doi.org/10.1242/dev.024802 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010667842
310 rdf:type schema:CreativeWork
311 https://doi.org/10.1242/jcs.069997 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013101237
312 rdf:type schema:CreativeWork
313 https://doi.org/10.1247/csf.13018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046711999
314 rdf:type schema:CreativeWork
315 https://doi.org/10.1371/journal.pone.0036431 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034941595
316 rdf:type schema:CreativeWork
317 https://doi.org/10.1371/journal.pone.0076853 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018375583
318 rdf:type schema:CreativeWork
319 https://doi.org/10.1523/jneurosci.1659-11.2011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034085318
320 rdf:type schema:CreativeWork
321 https://doi.org/10.1523/jneurosci.1692-04.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013217337
322 rdf:type schema:CreativeWork
323 https://doi.org/10.1523/jneurosci.3372-12.2013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021033622
324 rdf:type schema:CreativeWork
325 https://doi.org/10.7554/elife.05871 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042974041
326 rdf:type schema:CreativeWork
327 https://www.grid.ac/institutes/grid.258799.8 schema:alternateName Kyoto University
328 schema:name Department of Animal Development and Physiology, Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.
329 Department of Growth Regulation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
330 Imaging Platform for Spatio-Temporal Information, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
331 rdf:type schema:Organization
332 https://www.grid.ac/institutes/grid.288127.6 schema:alternateName National Institute of Genetics
333 schema:name Division of Molecular and Developmental Biology, National Institute of Genetics, and Department of Genetics, SOKENDAI, Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan.
334 rdf:type schema:Organization
335 https://www.grid.ac/institutes/grid.474692.a schema:alternateName RIKEN Center for Developmental Biology
336 schema:name Laboratory of Cell Asymmetry, RIKEN Center of Developmental Biology, Kobe 650-0047, Japan.
337 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...