Ultrastructural analysis of the decellularized cornea after interlamellar keratoplasty and microkeratome-assisted anterior lamellar keratoplasty in a rabbit model View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-09

AUTHORS

Yoshihide Hashimoto, Shinya Hattori, Shuji Sasaki, Takako Honda, Tsuyoshi Kimura, Seiichi Funamoto, Hisatoshi Kobayashi, Akio Kishida

ABSTRACT

The decellularized cornea has received considerable attention for use as an artificial cornea. The decellularized cornea is free from cellular components and other immunogens, but maintains the integrity of the extracellular matrix. However, the ultrastructure of the decellularized cornea has yet to be demonstrated in detail. We investigated the influence of high hydrostatic pressure (HHP) on the decellularization of the corneal ultrastructure and its involvement in transparency, and assessed the in vivo behaviour of the decellularized cornea using two animal transplantation models, in relation to remodelling of collagen fibrils. Decellularized corneas were prepared by the HHP method. The decellularized corneas were executed by haematoxylin and eosin and Masson's trichrome staining to demonstrate the complete removal of corneal cells. Transmission electron microscopy revealed that the ultrastructure of the decellularized cornea prepared by the HHP method was better maintained than that of the decellularized cornea prepared by the detergent method. The decellularized cornea after interlamellar keratoplasty and microkeratome-assisted anterior lamellar keratoplasty using a rabbit model was stable and remained transparent without ultrastructural alterations. We conclude that the superior properties of the decellularized cornea prepared by the HHP method were attributed to the preservation of the corneal ultrastructure. More... »

PAGES

27734

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep27734

DOI

http://dx.doi.org/10.1038/srep27734

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005356206

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27291975


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1113", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Ophthalmology and Optometry", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cornea", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Corneal Transplantation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Histocytological Preparation Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Electron, Transmission", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rabbits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Swine", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "National Institute for Materials Science", 
          "id": "https://www.grid.ac/institutes/grid.21941.3f", 
          "name": [
            "Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan.", 
            "World Premier International Research Center Initiative, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Ibaraki, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hashimoto", 
        "givenName": "Yoshihide", 
        "id": "sg:person.01142040277.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142040277.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute for Materials Science", 
          "id": "https://www.grid.ac/institutes/grid.21941.3f", 
          "name": [
            "World Premier International Research Center Initiative, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Ibaraki, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hattori", 
        "givenName": "Shinya", 
        "id": "sg:person.01153467462.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153467462.93"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tokyo Medical and Dental University", 
          "id": "https://www.grid.ac/institutes/grid.265073.5", 
          "name": [
            "World Premier International Research Center Initiative, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Ibaraki, Japan.", 
            "Department of Ophthalmology, Tokyo Medical and Dental University, Tokyo, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sasaki", 
        "givenName": "Shuji", 
        "id": "sg:person.0654677262.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654677262.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute for Materials Science", 
          "id": "https://www.grid.ac/institutes/grid.21941.3f", 
          "name": [
            "World Premier International Research Center Initiative, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Ibaraki, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Honda", 
        "givenName": "Takako", 
        "id": "sg:person.01105354262.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105354262.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute for Materials Science", 
          "id": "https://www.grid.ac/institutes/grid.21941.3f", 
          "name": [
            "Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan.", 
            "World Premier International Research Center Initiative, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Ibaraki, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kimura", 
        "givenName": "Tsuyoshi", 
        "id": "sg:person.01163650522.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163650522.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute for Materials Science", 
          "id": "https://www.grid.ac/institutes/grid.21941.3f", 
          "name": [
            "Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan.", 
            "World Premier International Research Center Initiative, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Ibaraki, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Funamoto", 
        "givenName": "Seiichi", 
        "id": "sg:person.01173116213.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173116213.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute for Materials Science", 
          "id": "https://www.grid.ac/institutes/grid.21941.3f", 
          "name": [
            "World Premier International Research Center Initiative, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Ibaraki, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kobayashi", 
        "givenName": "Hisatoshi", 
        "id": "sg:person.01070110657.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070110657.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute for Materials Science", 
          "id": "https://www.grid.ac/institutes/grid.21941.3f", 
          "name": [
            "Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan.", 
            "World Premier International Research Center Initiative, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Ibaraki, Japan."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kishida", 
        "givenName": "Akio", 
        "id": "sg:person.0714140254.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714140254.17"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1001/jamaophthalmol.2015.4776", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004758265"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1001/archopht.123.10.1319", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005493938"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bip.1969.360080306", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005670323"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bip.1969.360080306", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005670323"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/eye.1997.127", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006692578", 
          "https://doi.org/10.1038/eye.1997.127"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/eye.1997.127", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006692578", 
          "https://doi.org/10.1038/eye.1997.127"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2003.12.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014229349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0142-9612(01)00168-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015165227"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/bjophthalmol-2011-300539", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016057192"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m110.153965", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017478999"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/bjo.2008.142539", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019208500"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0131989", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020766193"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/icu.0000000000000049", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022116576"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/icu.0000000000000049", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022116576"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biomaterials.2006.02.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022430375"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.exer.2012.12.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027232041"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/jfb4030114", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027972971"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1113/jphysiol.1957.sp005758", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028212683"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1001/archopht.122.4.615", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028316715"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biomaterials.2010.01.122", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035794774"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/02713683.2015.1062114", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039805800"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/02713683.2015.1062114", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039805800"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1203/pdr.0b013e31816bdf54", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042453350", 
          "https://doi.org/10.1203/pdr.0b013e31816bdf54"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1203/pdr.0b013e31816bdf54", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042453350", 
          "https://doi.org/10.1203/pdr.0b013e31816bdf54"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00003226-200005000-00020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042530837"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00003226-200005000-00020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042530837"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/ijms13033229", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047795056"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/ico.0b013e3181a186dc", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051080957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/ico.0b013e3181a186dc", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051080957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/02713680600954278", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058314000"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077973833", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-09", 
    "datePublishedReg": "2016-09-01", 
    "description": "The decellularized cornea has received considerable attention for use as an artificial cornea. The decellularized cornea is free from cellular components and other immunogens, but maintains the integrity of the extracellular matrix. However, the ultrastructure of the decellularized cornea has yet to be demonstrated in detail. We investigated the influence of high hydrostatic pressure (HHP) on the decellularization of the corneal ultrastructure and its involvement in transparency, and assessed the in vivo behaviour of the decellularized cornea using two animal transplantation models, in relation to remodelling of collagen fibrils. Decellularized corneas were prepared by the HHP method. The decellularized corneas were executed by haematoxylin and eosin and Masson's trichrome staining to demonstrate the complete removal of corneal cells. Transmission electron microscopy revealed that the ultrastructure of the decellularized cornea prepared by the HHP method was better maintained than that of the decellularized cornea prepared by the detergent method. The decellularized cornea after interlamellar keratoplasty and microkeratome-assisted anterior lamellar keratoplasty using a rabbit model was stable and remained transparent without ultrastructural alterations. We conclude that the superior properties of the decellularized cornea prepared by the HHP method were attributed to the preservation of the corneal ultrastructure.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/srep27734", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "6"
      }
    ], 
    "name": "Ultrastructural analysis of the decellularized cornea after interlamellar keratoplasty and microkeratome-assisted anterior lamellar keratoplasty in a rabbit model", 
    "pagination": "27734", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "1984ac6dec46bd3879c19b5e54a100651f1ffcaae303038bdfe6dede8686b816"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "27291975"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/srep27734"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1005356206"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/srep27734", 
      "https://app.dimensions.ai/details/publication/pub.1005356206"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T02:18", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000549.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/srep/2016/160613/srep27734/full/srep27734.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/srep27734'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/srep27734'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/srep27734'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/srep27734'


 

This table displays all metadata directly associated to this object as RDF triples.

231 TRIPLES      21 PREDICATES      62 URIs      30 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/srep27734 schema:about N17c3413101df430ab1084c177f9c2744
2 N1e484df4ae914c2aab695a295b97280c
3 N349d357f92bd4467bf8ad08837c8cbb2
4 N36b03997e09949a28e253dd46c482ce4
5 N5eb5b5d16ef145fe956d9ff18663af21
6 N8464aad7cb95425ca58f851f0353bcd0
7 Na80f9d7d0f394283a62ce1a0e537c8d6
8 Naa251665c3d94d70ba1c535ac6f5d6e0
9 Nc6ad9f4c083543dd8a6eb384a83992b0
10 anzsrc-for:11
11 anzsrc-for:1113
12 schema:author N2a4ef5e4adf945b99725dc2a1a2959ae
13 schema:citation sg:pub.10.1038/eye.1997.127
14 sg:pub.10.1203/pdr.0b013e31816bdf54
15 https://app.dimensions.ai/details/publication/pub.1077973833
16 https://doi.org/10.1001/archopht.122.4.615
17 https://doi.org/10.1001/archopht.123.10.1319
18 https://doi.org/10.1001/jamaophthalmol.2015.4776
19 https://doi.org/10.1002/bip.1969.360080306
20 https://doi.org/10.1016/j.biomaterials.2006.02.014
21 https://doi.org/10.1016/j.biomaterials.2010.01.122
22 https://doi.org/10.1016/j.exer.2012.12.012
23 https://doi.org/10.1016/j.jmb.2003.12.001
24 https://doi.org/10.1016/s0142-9612(01)00168-5
25 https://doi.org/10.1074/jbc.m110.153965
26 https://doi.org/10.1080/02713680600954278
27 https://doi.org/10.1097/00003226-200005000-00020
28 https://doi.org/10.1097/ico.0b013e3181a186dc
29 https://doi.org/10.1097/icu.0000000000000049
30 https://doi.org/10.1113/jphysiol.1957.sp005758
31 https://doi.org/10.1136/bjo.2008.142539
32 https://doi.org/10.1136/bjophthalmol-2011-300539
33 https://doi.org/10.1371/journal.pone.0131989
34 https://doi.org/10.3109/02713683.2015.1062114
35 https://doi.org/10.3390/ijms13033229
36 https://doi.org/10.3390/jfb4030114
37 schema:datePublished 2016-09
38 schema:datePublishedReg 2016-09-01
39 schema:description The decellularized cornea has received considerable attention for use as an artificial cornea. The decellularized cornea is free from cellular components and other immunogens, but maintains the integrity of the extracellular matrix. However, the ultrastructure of the decellularized cornea has yet to be demonstrated in detail. We investigated the influence of high hydrostatic pressure (HHP) on the decellularization of the corneal ultrastructure and its involvement in transparency, and assessed the in vivo behaviour of the decellularized cornea using two animal transplantation models, in relation to remodelling of collagen fibrils. Decellularized corneas were prepared by the HHP method. The decellularized corneas were executed by haematoxylin and eosin and Masson's trichrome staining to demonstrate the complete removal of corneal cells. Transmission electron microscopy revealed that the ultrastructure of the decellularized cornea prepared by the HHP method was better maintained than that of the decellularized cornea prepared by the detergent method. The decellularized cornea after interlamellar keratoplasty and microkeratome-assisted anterior lamellar keratoplasty using a rabbit model was stable and remained transparent without ultrastructural alterations. We conclude that the superior properties of the decellularized cornea prepared by the HHP method were attributed to the preservation of the corneal ultrastructure.
40 schema:genre research_article
41 schema:inLanguage en
42 schema:isAccessibleForFree true
43 schema:isPartOf N01abf4612d764db6a0c5b10bdc6a7e4f
44 N813e12bd99f34e128e68145af7a50a45
45 sg:journal.1045337
46 schema:name Ultrastructural analysis of the decellularized cornea after interlamellar keratoplasty and microkeratome-assisted anterior lamellar keratoplasty in a rabbit model
47 schema:pagination 27734
48 schema:productId N0f2026cbd55f4ce89b42d2526fcbc28f
49 N2edb2c8bde90476d8c99f8ebe615544e
50 N93f713e75444427ab63e08e7be69d426
51 Na8689223723e4c5bb4fb69db5a88f1b4
52 Nac6dc4d3a1f746f699b4fa1fc2e6ed6d
53 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005356206
54 https://doi.org/10.1038/srep27734
55 schema:sdDatePublished 2019-04-11T02:18
56 schema:sdLicense https://scigraph.springernature.com/explorer/license/
57 schema:sdPublisher N93eee3eb411143758408790724a41ba3
58 schema:url http://www.nature.com/srep/2016/160613/srep27734/full/srep27734.html
59 sgo:license sg:explorer/license/
60 sgo:sdDataset articles
61 rdf:type schema:ScholarlyArticle
62 N01abf4612d764db6a0c5b10bdc6a7e4f schema:issueNumber 1
63 rdf:type schema:PublicationIssue
64 N0f2026cbd55f4ce89b42d2526fcbc28f schema:name readcube_id
65 schema:value 1984ac6dec46bd3879c19b5e54a100651f1ffcaae303038bdfe6dede8686b816
66 rdf:type schema:PropertyValue
67 N17c3413101df430ab1084c177f9c2744 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Rabbits
69 rdf:type schema:DefinedTerm
70 N1e484df4ae914c2aab695a295b97280c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Histocytological Preparation Techniques
72 rdf:type schema:DefinedTerm
73 N2a4ef5e4adf945b99725dc2a1a2959ae rdf:first sg:person.01142040277.11
74 rdf:rest Na1da3c210fe94abeae02ae961570749e
75 N2edb2c8bde90476d8c99f8ebe615544e schema:name nlm_unique_id
76 schema:value 101563288
77 rdf:type schema:PropertyValue
78 N349d357f92bd4467bf8ad08837c8cbb2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Cornea
80 rdf:type schema:DefinedTerm
81 N36b03997e09949a28e253dd46c482ce4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Swine
83 rdf:type schema:DefinedTerm
84 N4813b522b6aa45a4a879a877856b0630 rdf:first sg:person.01070110657.12
85 rdf:rest N798fb64c178b43998a5ab80ed80b303f
86 N5b4f127a7d8144208d3995942d2bd76b rdf:first sg:person.01173116213.44
87 rdf:rest N4813b522b6aa45a4a879a877856b0630
88 N5eb5b5d16ef145fe956d9ff18663af21 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Models, Animal
90 rdf:type schema:DefinedTerm
91 N798fb64c178b43998a5ab80ed80b303f rdf:first sg:person.0714140254.17
92 rdf:rest rdf:nil
93 N813e12bd99f34e128e68145af7a50a45 schema:volumeNumber 6
94 rdf:type schema:PublicationVolume
95 N8464aad7cb95425ca58f851f0353bcd0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Animals
97 rdf:type schema:DefinedTerm
98 N93eee3eb411143758408790724a41ba3 schema:name Springer Nature - SN SciGraph project
99 rdf:type schema:Organization
100 N93f713e75444427ab63e08e7be69d426 schema:name pubmed_id
101 schema:value 27291975
102 rdf:type schema:PropertyValue
103 Na1da3c210fe94abeae02ae961570749e rdf:first sg:person.01153467462.93
104 rdf:rest Nc94301099cee4d48a9bcc87e972f7240
105 Na80f9d7d0f394283a62ce1a0e537c8d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Microscopy, Electron, Transmission
107 rdf:type schema:DefinedTerm
108 Na8689223723e4c5bb4fb69db5a88f1b4 schema:name dimensions_id
109 schema:value pub.1005356206
110 rdf:type schema:PropertyValue
111 Naa251665c3d94d70ba1c535ac6f5d6e0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Corneal Transplantation
113 rdf:type schema:DefinedTerm
114 Nac6dc4d3a1f746f699b4fa1fc2e6ed6d schema:name doi
115 schema:value 10.1038/srep27734
116 rdf:type schema:PropertyValue
117 Nc6ad9f4c083543dd8a6eb384a83992b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Humans
119 rdf:type schema:DefinedTerm
120 Nc94301099cee4d48a9bcc87e972f7240 rdf:first sg:person.0654677262.49
121 rdf:rest Nec0acdb628314c97ac9822aa0253fff0
122 Nd72474b72bf048c79b6f3fb63bdc19b4 rdf:first sg:person.01163650522.33
123 rdf:rest N5b4f127a7d8144208d3995942d2bd76b
124 Nec0acdb628314c97ac9822aa0253fff0 rdf:first sg:person.01105354262.30
125 rdf:rest Nd72474b72bf048c79b6f3fb63bdc19b4
126 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
127 schema:name Medical and Health Sciences
128 rdf:type schema:DefinedTerm
129 anzsrc-for:1113 schema:inDefinedTermSet anzsrc-for:
130 schema:name Ophthalmology and Optometry
131 rdf:type schema:DefinedTerm
132 sg:journal.1045337 schema:issn 2045-2322
133 schema:name Scientific Reports
134 rdf:type schema:Periodical
135 sg:person.01070110657.12 schema:affiliation https://www.grid.ac/institutes/grid.21941.3f
136 schema:familyName Kobayashi
137 schema:givenName Hisatoshi
138 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070110657.12
139 rdf:type schema:Person
140 sg:person.01105354262.30 schema:affiliation https://www.grid.ac/institutes/grid.21941.3f
141 schema:familyName Honda
142 schema:givenName Takako
143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105354262.30
144 rdf:type schema:Person
145 sg:person.01142040277.11 schema:affiliation https://www.grid.ac/institutes/grid.21941.3f
146 schema:familyName Hashimoto
147 schema:givenName Yoshihide
148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142040277.11
149 rdf:type schema:Person
150 sg:person.01153467462.93 schema:affiliation https://www.grid.ac/institutes/grid.21941.3f
151 schema:familyName Hattori
152 schema:givenName Shinya
153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153467462.93
154 rdf:type schema:Person
155 sg:person.01163650522.33 schema:affiliation https://www.grid.ac/institutes/grid.21941.3f
156 schema:familyName Kimura
157 schema:givenName Tsuyoshi
158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163650522.33
159 rdf:type schema:Person
160 sg:person.01173116213.44 schema:affiliation https://www.grid.ac/institutes/grid.21941.3f
161 schema:familyName Funamoto
162 schema:givenName Seiichi
163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173116213.44
164 rdf:type schema:Person
165 sg:person.0654677262.49 schema:affiliation https://www.grid.ac/institutes/grid.265073.5
166 schema:familyName Sasaki
167 schema:givenName Shuji
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654677262.49
169 rdf:type schema:Person
170 sg:person.0714140254.17 schema:affiliation https://www.grid.ac/institutes/grid.21941.3f
171 schema:familyName Kishida
172 schema:givenName Akio
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714140254.17
174 rdf:type schema:Person
175 sg:pub.10.1038/eye.1997.127 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006692578
176 https://doi.org/10.1038/eye.1997.127
177 rdf:type schema:CreativeWork
178 sg:pub.10.1203/pdr.0b013e31816bdf54 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042453350
179 https://doi.org/10.1203/pdr.0b013e31816bdf54
180 rdf:type schema:CreativeWork
181 https://app.dimensions.ai/details/publication/pub.1077973833 schema:CreativeWork
182 https://doi.org/10.1001/archopht.122.4.615 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028316715
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1001/archopht.123.10.1319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005493938
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1001/jamaophthalmol.2015.4776 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004758265
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1002/bip.1969.360080306 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005670323
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/j.biomaterials.2006.02.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022430375
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/j.biomaterials.2010.01.122 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035794774
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/j.exer.2012.12.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027232041
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/j.jmb.2003.12.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014229349
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/s0142-9612(01)00168-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015165227
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1074/jbc.m110.153965 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017478999
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1080/02713680600954278 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058314000
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1097/00003226-200005000-00020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042530837
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1097/ico.0b013e3181a186dc schema:sameAs https://app.dimensions.ai/details/publication/pub.1051080957
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1097/icu.0000000000000049 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022116576
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1113/jphysiol.1957.sp005758 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028212683
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1136/bjo.2008.142539 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019208500
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1136/bjophthalmol-2011-300539 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016057192
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1371/journal.pone.0131989 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020766193
217 rdf:type schema:CreativeWork
218 https://doi.org/10.3109/02713683.2015.1062114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039805800
219 rdf:type schema:CreativeWork
220 https://doi.org/10.3390/ijms13033229 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047795056
221 rdf:type schema:CreativeWork
222 https://doi.org/10.3390/jfb4030114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027972971
223 rdf:type schema:CreativeWork
224 https://www.grid.ac/institutes/grid.21941.3f schema:alternateName National Institute for Materials Science
225 schema:name Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan.
226 World Premier International Research Center Initiative, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Ibaraki, Japan.
227 rdf:type schema:Organization
228 https://www.grid.ac/institutes/grid.265073.5 schema:alternateName Tokyo Medical and Dental University
229 schema:name Department of Ophthalmology, Tokyo Medical and Dental University, Tokyo, Japan.
230 World Premier International Research Center Initiative, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Ibaraki, Japan.
231 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...