Fungal endophytes of Catharanthus roseus enhance vindoline content by modulating structural and regulatory genes related to terpenoid indole alkaloid biosynthesis View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-05-25

AUTHORS

Shiv S. Pandey, Sucheta Singh, C. S. Vivek Babu, Karuna Shanker, N. K. Srivastava, Ashutosh K. Shukla, Alok Kalra

ABSTRACT

Not much is known about the mechanism of endophyte-mediated induction of secondary metabolite production in Catharanthus roseus. In the present study two fungal endophytes, Curvularia sp. CATDLF5 and Choanephora infundibulifera CATDLF6 were isolated from the leaves of the plant that were found to enhance vindoline content by 229-403%. The isolated endophytes did not affect the primary metabolism of the plant as the maximum quantum efficiency of PSII, net CO2 assimilation, plant biomass and starch content of endophyte-inoculated plants was similar to endophyte-free control plants. Expression of terpenoid indole alkaloid (TIA) pathway genes, geraniol 10-hydroxylase (G10H), tryptophan decarboxylase (TDC), strictosidine synthase (STR), 16-hydoxytabersonine-O-methyltransferase (16OMT), desacetoxyvindoline-4-hydroxylase (D4H), deacetylvindoline-4-O-acetyltransferase (DAT) were upregulated in endophyte-inoculated plants. Endophyte inoculation upregulated the expression of the gene for transcriptional activator octadecanoid-responsive Catharanthus AP2-domain protein (ORCA3) and downregulated the expression of Cys2/His2-type zinc finger protein family transcriptional repressors (ZCTs). The gene for the vacuolar class III peroxidase (PRX1), responsible for coupling vindoline and catharanthine, was upregulated in endophyte-inoculated plants. These endophytes may enhance vindoline production by modulating the expression of key structural and regulatory genes of vindoline biosynthesis without affecting the primary metabolism of the host plant. More... »

PAGES

26583

References to SciGraph publications

  • 2013-02-17. In Vitro α-Glucosidase Inhibition and Antioxidative Potential of an Endophyte Species (Streptomyces sp. Loyola UGC) Isolated from Datura stramonium L in CURRENT MICROBIOLOGY
  • 2012-08-07. CrMPK3, a mitogen activated protein kinase from Catharanthus roseus and its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids in BMC PLANT BIOLOGY
  • 2013-05-22. Colonization of Madagascar periwinkle (Catharanthus roseus), by endophytes encoding gfp marker in ARCHIVES OF MICROBIOLOGY
  • 2011-11-19. Endophytic Methylobacterium extorquens expresses a heterologous β-1,4-endoglucanase A (EglA) in Catharanthus roseus seedlings, a model host plant for Xylella fastidiosa in WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
  • 2004. Chlorophyll a Fluorescence, A Signature of Photosynthesis in NONE
  • 2008-06-05. Analyzing real-time PCR data by the comparative CT method in NATURE PROTOCOLS
  • 2007-05-04. Transcription factors involved in terpenoid indole alkaloid biosynthesis in Catharanthus roseus in PHYTOCHEMISTRY REVIEWS
  • 2013-10-08. The ORCA2 transcription factor plays a key role in regulation of the terpenoid indole alkaloid pathway in BMC PLANT BIOLOGY
  • 2011-09-09. An endophytic fungus from Azadirachta indica A. Juss. that produces azadirachtin in WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
  • 2001-03. Catharanthus roseus G-box binding factors 1 and 2 act as repressors of strictosidine synthase gene expression in cell cultures in PLANT MOLECULAR BIOLOGY
  • 2004. Chlorophyll Fluorescence as a Tool to Monitor Plant Response to the Environment in CHLOROPHYLL A FLUORESCENCE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/srep26583

    DOI

    http://dx.doi.org/10.1038/srep26583

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1045979555

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27220774


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Plant Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Catharanthus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Endophytes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Regulation, Plant", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mucorales", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plant Leaves", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Vinblastine", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Microbial Technology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Microbial Technology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pandey", 
            "givenName": "Shiv S.", 
            "id": "sg:person.01342510575.14", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342510575.14"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Microbial Technology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Microbial Technology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Singh", 
            "givenName": "Sucheta", 
            "id": "sg:person.0617104475.73", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617104475.73"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "CSIR, -Cent, ral Institute of Medicinal and Aromatic Plants, Research Centre, Allalasandra, GKVK Post, Bangalore-560065, India", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "CSIR, -Cent, ral Institute of Medicinal and Aromatic Plants, Research Centre, Allalasandra, GKVK Post, Bangalore-560065, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Babu", 
            "givenName": "C. S. Vivek", 
            "id": "sg:person.0665217675.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665217675.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Analytical Chemistry Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Analytical Chemistry Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shanker", 
            "givenName": "Karuna", 
            "id": "sg:person.0650767015.23", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0650767015.23"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Plant Physiology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Plant Physiology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Srivastava", 
            "givenName": "N. K.", 
            "id": "sg:person.01106572753.82", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106572753.82"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shukla", 
            "givenName": "Ashutosh K.", 
            "id": "sg:person.0746716663.99", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746716663.99"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Microbial Technology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Microbial Technology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kalra", 
            "givenName": "Alok", 
            "id": "sg:person.01157615463.85", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01157615463.85"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/s00203-013-0897-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024562742", 
              "https://doi.org/10.1007/s00203-013-0897-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00284-013-0329-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047085038", 
              "https://doi.org/10.1007/s00284-013-0329-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2008.73", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005355981", 
              "https://doi.org/10.1038/nprot.2008.73"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2229-13-155", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035150405", 
              "https://doi.org/10.1186/1471-2229-13-155"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1010650906695", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036417841", 
              "https://doi.org/10.1023/a:1010650906695"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11274-011-0949-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010409010", 
              "https://doi.org/10.1007/s11274-011-0949-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11101-006-9051-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032454225", 
              "https://doi.org/10.1007/s11101-006-9051-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-4020-3218-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005627479", 
              "https://doi.org/10.1007/978-1-4020-3218-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-4020-3218-9_22", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044610722", 
              "https://doi.org/10.1007/978-1-4020-3218-9_22"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11274-011-0876-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031074869", 
              "https://doi.org/10.1007/s11274-011-0876-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2229-12-134", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009173528", 
              "https://doi.org/10.1186/1471-2229-12-134"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2016-05-25", 
        "datePublishedReg": "2016-05-25", 
        "description": "Not much is known about the mechanism of endophyte-mediated induction of secondary metabolite production in Catharanthus roseus. In the present study two fungal endophytes, Curvularia sp. CATDLF5 and Choanephora infundibulifera CATDLF6 were isolated from the leaves of the plant that were found to enhance vindoline content by 229-403%. The isolated endophytes did not affect the primary metabolism of the plant as the maximum quantum efficiency of PSII, net CO2 assimilation, plant biomass and starch content of endophyte-inoculated plants was similar to endophyte-free control plants. Expression of terpenoid indole alkaloid (TIA) pathway genes, geraniol 10-hydroxylase (G10H), tryptophan decarboxylase (TDC), strictosidine synthase (STR), 16-hydoxytabersonine-O-methyltransferase (16OMT), desacetoxyvindoline-4-hydroxylase (D4H), deacetylvindoline-4-O-acetyltransferase (DAT) were upregulated in endophyte-inoculated plants. Endophyte inoculation upregulated the expression of the gene for transcriptional activator octadecanoid-responsive Catharanthus AP2-domain protein (ORCA3) and downregulated the expression of Cys2/His2-type zinc finger protein family transcriptional repressors (ZCTs). The gene for the vacuolar class III peroxidase (PRX1), responsible for coupling vindoline and catharanthine, was upregulated in endophyte-inoculated plants. These endophytes may enhance vindoline production by modulating the expression of key structural and regulatory genes of vindoline biosynthesis without affecting the primary metabolism of the host plant.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/srep26583", 
        "inLanguage": "en", 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1045337", 
            "issn": [
              "2045-2322"
            ], 
            "name": "Scientific Reports", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "6"
          }
        ], 
        "keywords": [
          "endophyte-inoculated plants", 
          "primary metabolism", 
          "fungal endophytes", 
          "regulatory genes", 
          "strictosidine synthase", 
          "terpenoid indole alkaloid pathway genes", 
          "tryptophan decarboxylase", 
          "vacuolar class III peroxidase", 
          "terpenoid indole alkaloid biosynthesis", 
          "AP2 domain protein", 
          "class III peroxidases", 
          "net CO2 assimilation", 
          "indole alkaloid biosynthesis", 
          "vindoline content", 
          "secondary metabolite production", 
          "host plants", 
          "CO2 assimilation", 
          "transcriptional repressor", 
          "endophyte inoculation", 
          "alkaloid biosynthesis", 
          "vindoline biosynthesis", 
          "control plants", 
          "maximum quantum efficiency", 
          "plant biomass", 
          "pathway genes", 
          "endophytes", 
          "metabolite production", 
          "Catharanthus roseus", 
          "genes", 
          "vindoline production", 
          "Curvularia sp", 
          "plants", 
          "biosynthesis", 
          "expression", 
          "starch content", 
          "metabolism", 
          "repressor", 
          "PSII", 
          "methyltransferase", 
          "leaves", 
          "roseus", 
          "protein", 
          "sp", 
          "synthase", 
          "vindoline", 
          "biomass", 
          "catharanthine", 
          "production", 
          "decarboxylase", 
          "acetyltransferase", 
          "assimilation", 
          "inoculation", 
          "induction", 
          "peroxidase", 
          "mechanism", 
          "content", 
          "geraniol", 
          "efficiency", 
          "quantum efficiency", 
          "endophyte-mediated induction", 
          "present study two fungal endophytes", 
          "study two fungal endophytes", 
          "two fungal endophytes", 
          "CATDLF5", 
          "Choanephora infundibulifera CATDLF6", 
          "infundibulifera CATDLF6", 
          "CATDLF6", 
          "endophyte-free control plants", 
          "indole alkaloid (TIA) pathway genes", 
          "alkaloid (TIA) pathway genes", 
          "transcriptional activator octadecanoid-responsive Catharanthus AP2-domain protein", 
          "activator octadecanoid-responsive Catharanthus AP2-domain protein", 
          "octadecanoid-responsive Catharanthus AP2-domain protein", 
          "Catharanthus AP2-domain protein", 
          "Cys2/His2-type zinc finger protein family transcriptional repressors", 
          "His2-type zinc finger protein family transcriptional repressors", 
          "zinc finger protein family transcriptional repressors", 
          "finger protein family transcriptional repressors", 
          "protein family transcriptional repressors", 
          "family transcriptional repressors", 
          "III peroxidase", 
          "Catharanthus roseus enhance vindoline content", 
          "roseus enhance vindoline content", 
          "enhance vindoline content"
        ], 
        "name": "Fungal endophytes of Catharanthus roseus enhance vindoline content by modulating structural and regulatory genes related to terpenoid indole alkaloid biosynthesis", 
        "pagination": "26583", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1045979555"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/srep26583"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "27220774"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/srep26583", 
          "https://app.dimensions.ai/details/publication/pub.1045979555"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2021-11-01T18:27", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20211101/entities/gbq_results/article/article_709.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/srep26583"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/srep26583'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/srep26583'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/srep26583'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/srep26583'


     

    This table displays all metadata directly associated to this object as RDF triples.

    272 TRIPLES      22 PREDICATES      129 URIs      108 LITERALS      13 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/srep26583 schema:about N1b01d602831047ddb0561f1be7cb2fb9
    2 N397b5e8d33984f39af0a7fb460646a73
    3 N4893563351754e7485dcbf19a5dadc45
    4 N865c49aa0d224a2aad44bb66029d622f
    5 N97461a35c8514bcfbd8478e8d5411e0c
    6 Nf4156fab80cf4dd585144cef2f561ef0
    7 anzsrc-for:06
    8 anzsrc-for:0601
    9 anzsrc-for:0604
    10 anzsrc-for:0607
    11 schema:author N5e29ebddbb204ebfa47bbf6047c580df
    12 schema:citation sg:pub.10.1007/978-1-4020-3218-9
    13 sg:pub.10.1007/978-1-4020-3218-9_22
    14 sg:pub.10.1007/s00203-013-0897-3
    15 sg:pub.10.1007/s00284-013-0329-2
    16 sg:pub.10.1007/s11101-006-9051-z
    17 sg:pub.10.1007/s11274-011-0876-2
    18 sg:pub.10.1007/s11274-011-0949-2
    19 sg:pub.10.1023/a:1010650906695
    20 sg:pub.10.1038/nprot.2008.73
    21 sg:pub.10.1186/1471-2229-12-134
    22 sg:pub.10.1186/1471-2229-13-155
    23 schema:datePublished 2016-05-25
    24 schema:datePublishedReg 2016-05-25
    25 schema:description Not much is known about the mechanism of endophyte-mediated induction of secondary metabolite production in Catharanthus roseus. In the present study two fungal endophytes, Curvularia sp. CATDLF5 and Choanephora infundibulifera CATDLF6 were isolated from the leaves of the plant that were found to enhance vindoline content by 229-403%. The isolated endophytes did not affect the primary metabolism of the plant as the maximum quantum efficiency of PSII, net CO2 assimilation, plant biomass and starch content of endophyte-inoculated plants was similar to endophyte-free control plants. Expression of terpenoid indole alkaloid (TIA) pathway genes, geraniol 10-hydroxylase (G10H), tryptophan decarboxylase (TDC), strictosidine synthase (STR), 16-hydoxytabersonine-O-methyltransferase (16OMT), desacetoxyvindoline-4-hydroxylase (D4H), deacetylvindoline-4-O-acetyltransferase (DAT) were upregulated in endophyte-inoculated plants. Endophyte inoculation upregulated the expression of the gene for transcriptional activator octadecanoid-responsive Catharanthus AP2-domain protein (ORCA3) and downregulated the expression of Cys2/His2-type zinc finger protein family transcriptional repressors (ZCTs). The gene for the vacuolar class III peroxidase (PRX1), responsible for coupling vindoline and catharanthine, was upregulated in endophyte-inoculated plants. These endophytes may enhance vindoline production by modulating the expression of key structural and regulatory genes of vindoline biosynthesis without affecting the primary metabolism of the host plant.
    26 schema:genre article
    27 schema:inLanguage en
    28 schema:isAccessibleForFree true
    29 schema:isPartOf N8006cdf51053494bb31a6bbd6f8d73dd
    30 N9f9c9ce57961420faa28f953b0fd8538
    31 sg:journal.1045337
    32 schema:keywords AP2 domain protein
    33 CATDLF5
    34 CATDLF6
    35 CO2 assimilation
    36 Catharanthus AP2-domain protein
    37 Catharanthus roseus
    38 Catharanthus roseus enhance vindoline content
    39 Choanephora infundibulifera CATDLF6
    40 Curvularia sp
    41 Cys2/His2-type zinc finger protein family transcriptional repressors
    42 His2-type zinc finger protein family transcriptional repressors
    43 III peroxidase
    44 PSII
    45 acetyltransferase
    46 activator octadecanoid-responsive Catharanthus AP2-domain protein
    47 alkaloid (TIA) pathway genes
    48 alkaloid biosynthesis
    49 assimilation
    50 biomass
    51 biosynthesis
    52 catharanthine
    53 class III peroxidases
    54 content
    55 control plants
    56 decarboxylase
    57 efficiency
    58 endophyte inoculation
    59 endophyte-free control plants
    60 endophyte-inoculated plants
    61 endophyte-mediated induction
    62 endophytes
    63 enhance vindoline content
    64 expression
    65 family transcriptional repressors
    66 finger protein family transcriptional repressors
    67 fungal endophytes
    68 genes
    69 geraniol
    70 host plants
    71 indole alkaloid (TIA) pathway genes
    72 indole alkaloid biosynthesis
    73 induction
    74 infundibulifera CATDLF6
    75 inoculation
    76 leaves
    77 maximum quantum efficiency
    78 mechanism
    79 metabolism
    80 metabolite production
    81 methyltransferase
    82 net CO2 assimilation
    83 octadecanoid-responsive Catharanthus AP2-domain protein
    84 pathway genes
    85 peroxidase
    86 plant biomass
    87 plants
    88 present study two fungal endophytes
    89 primary metabolism
    90 production
    91 protein
    92 protein family transcriptional repressors
    93 quantum efficiency
    94 regulatory genes
    95 repressor
    96 roseus
    97 roseus enhance vindoline content
    98 secondary metabolite production
    99 sp
    100 starch content
    101 strictosidine synthase
    102 study two fungal endophytes
    103 synthase
    104 terpenoid indole alkaloid biosynthesis
    105 terpenoid indole alkaloid pathway genes
    106 transcriptional activator octadecanoid-responsive Catharanthus AP2-domain protein
    107 transcriptional repressor
    108 tryptophan decarboxylase
    109 two fungal endophytes
    110 vacuolar class III peroxidase
    111 vindoline
    112 vindoline biosynthesis
    113 vindoline content
    114 vindoline production
    115 zinc finger protein family transcriptional repressors
    116 schema:name Fungal endophytes of Catharanthus roseus enhance vindoline content by modulating structural and regulatory genes related to terpenoid indole alkaloid biosynthesis
    117 schema:pagination 26583
    118 schema:productId N6c8b340918eb4779acddc0b5d2da0bca
    119 Ne8a8e99708244942a7d84212fbcb24fe
    120 Nef491fad7b9c4233b48867491394ca3d
    121 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045979555
    122 https://doi.org/10.1038/srep26583
    123 schema:sdDatePublished 2021-11-01T18:27
    124 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    125 schema:sdPublisher N167f6c40842741419c8492680af7b297
    126 schema:url https://doi.org/10.1038/srep26583
    127 sgo:license sg:explorer/license/
    128 sgo:sdDataset articles
    129 rdf:type schema:ScholarlyArticle
    130 N06edb8198ff345d9a03faea96fbe46ed rdf:first sg:person.0665217675.01
    131 rdf:rest Nb75a4c66bf8141be966d25bce3c71912
    132 N167f6c40842741419c8492680af7b297 schema:name Springer Nature - SN SciGraph project
    133 rdf:type schema:Organization
    134 N1b01d602831047ddb0561f1be7cb2fb9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    135 schema:name Plant Leaves
    136 rdf:type schema:DefinedTerm
    137 N397b5e8d33984f39af0a7fb460646a73 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    138 schema:name Gene Expression Regulation, Plant
    139 rdf:type schema:DefinedTerm
    140 N4893563351754e7485dcbf19a5dadc45 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Mucorales
    142 rdf:type schema:DefinedTerm
    143 N5e29ebddbb204ebfa47bbf6047c580df rdf:first sg:person.01342510575.14
    144 rdf:rest N6cb3d267d3ed44bb8d63085a82a8e54f
    145 N6c8b340918eb4779acddc0b5d2da0bca schema:name doi
    146 schema:value 10.1038/srep26583
    147 rdf:type schema:PropertyValue
    148 N6cb3d267d3ed44bb8d63085a82a8e54f rdf:first sg:person.0617104475.73
    149 rdf:rest N06edb8198ff345d9a03faea96fbe46ed
    150 N8006cdf51053494bb31a6bbd6f8d73dd schema:volumeNumber 6
    151 rdf:type schema:PublicationVolume
    152 N865c49aa0d224a2aad44bb66029d622f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Vinblastine
    154 rdf:type schema:DefinedTerm
    155 N97461a35c8514bcfbd8478e8d5411e0c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name Catharanthus
    157 rdf:type schema:DefinedTerm
    158 N995e0302bdcd41c696e539e03b38978f rdf:first sg:person.01106572753.82
    159 rdf:rest Nb4bedc30129645aa89aff26e1f8098be
    160 N9f9c9ce57961420faa28f953b0fd8538 schema:issueNumber 1
    161 rdf:type schema:PublicationIssue
    162 Nb0f10eaa2faa4393aaf950ae6a41bb8d rdf:first sg:person.01157615463.85
    163 rdf:rest rdf:nil
    164 Nb4bedc30129645aa89aff26e1f8098be rdf:first sg:person.0746716663.99
    165 rdf:rest Nb0f10eaa2faa4393aaf950ae6a41bb8d
    166 Nb75a4c66bf8141be966d25bce3c71912 rdf:first sg:person.0650767015.23
    167 rdf:rest N995e0302bdcd41c696e539e03b38978f
    168 Ne8a8e99708244942a7d84212fbcb24fe schema:name pubmed_id
    169 schema:value 27220774
    170 rdf:type schema:PropertyValue
    171 Nef491fad7b9c4233b48867491394ca3d schema:name dimensions_id
    172 schema:value pub.1045979555
    173 rdf:type schema:PropertyValue
    174 Nf4156fab80cf4dd585144cef2f561ef0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    175 schema:name Endophytes
    176 rdf:type schema:DefinedTerm
    177 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    178 schema:name Biological Sciences
    179 rdf:type schema:DefinedTerm
    180 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    181 schema:name Biochemistry and Cell Biology
    182 rdf:type schema:DefinedTerm
    183 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    184 schema:name Genetics
    185 rdf:type schema:DefinedTerm
    186 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
    187 schema:name Plant Biology
    188 rdf:type schema:DefinedTerm
    189 sg:journal.1045337 schema:issn 2045-2322
    190 schema:name Scientific Reports
    191 schema:publisher Springer Nature
    192 rdf:type schema:Periodical
    193 sg:person.01106572753.82 schema:affiliation grid-institutes:grid.417631.6
    194 schema:familyName Srivastava
    195 schema:givenName N. K.
    196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106572753.82
    197 rdf:type schema:Person
    198 sg:person.01157615463.85 schema:affiliation grid-institutes:grid.417631.6
    199 schema:familyName Kalra
    200 schema:givenName Alok
    201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01157615463.85
    202 rdf:type schema:Person
    203 sg:person.01342510575.14 schema:affiliation grid-institutes:grid.417631.6
    204 schema:familyName Pandey
    205 schema:givenName Shiv S.
    206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342510575.14
    207 rdf:type schema:Person
    208 sg:person.0617104475.73 schema:affiliation grid-institutes:grid.417631.6
    209 schema:familyName Singh
    210 schema:givenName Sucheta
    211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617104475.73
    212 rdf:type schema:Person
    213 sg:person.0650767015.23 schema:affiliation grid-institutes:grid.417631.6
    214 schema:familyName Shanker
    215 schema:givenName Karuna
    216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0650767015.23
    217 rdf:type schema:Person
    218 sg:person.0665217675.01 schema:affiliation grid-institutes:None
    219 schema:familyName Babu
    220 schema:givenName C. S. Vivek
    221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665217675.01
    222 rdf:type schema:Person
    223 sg:person.0746716663.99 schema:affiliation grid-institutes:grid.417631.6
    224 schema:familyName Shukla
    225 schema:givenName Ashutosh K.
    226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746716663.99
    227 rdf:type schema:Person
    228 sg:pub.10.1007/978-1-4020-3218-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005627479
    229 https://doi.org/10.1007/978-1-4020-3218-9
    230 rdf:type schema:CreativeWork
    231 sg:pub.10.1007/978-1-4020-3218-9_22 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044610722
    232 https://doi.org/10.1007/978-1-4020-3218-9_22
    233 rdf:type schema:CreativeWork
    234 sg:pub.10.1007/s00203-013-0897-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024562742
    235 https://doi.org/10.1007/s00203-013-0897-3
    236 rdf:type schema:CreativeWork
    237 sg:pub.10.1007/s00284-013-0329-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047085038
    238 https://doi.org/10.1007/s00284-013-0329-2
    239 rdf:type schema:CreativeWork
    240 sg:pub.10.1007/s11101-006-9051-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1032454225
    241 https://doi.org/10.1007/s11101-006-9051-z
    242 rdf:type schema:CreativeWork
    243 sg:pub.10.1007/s11274-011-0876-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031074869
    244 https://doi.org/10.1007/s11274-011-0876-2
    245 rdf:type schema:CreativeWork
    246 sg:pub.10.1007/s11274-011-0949-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010409010
    247 https://doi.org/10.1007/s11274-011-0949-2
    248 rdf:type schema:CreativeWork
    249 sg:pub.10.1023/a:1010650906695 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036417841
    250 https://doi.org/10.1023/a:1010650906695
    251 rdf:type schema:CreativeWork
    252 sg:pub.10.1038/nprot.2008.73 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005355981
    253 https://doi.org/10.1038/nprot.2008.73
    254 rdf:type schema:CreativeWork
    255 sg:pub.10.1186/1471-2229-12-134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009173528
    256 https://doi.org/10.1186/1471-2229-12-134
    257 rdf:type schema:CreativeWork
    258 sg:pub.10.1186/1471-2229-13-155 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035150405
    259 https://doi.org/10.1186/1471-2229-13-155
    260 rdf:type schema:CreativeWork
    261 grid-institutes:None schema:alternateName CSIR, -Cent, ral Institute of Medicinal and Aromatic Plants, Research Centre, Allalasandra, GKVK Post, Bangalore-560065, India
    262 schema:name CSIR, -Cent, ral Institute of Medicinal and Aromatic Plants, Research Centre, Allalasandra, GKVK Post, Bangalore-560065, India
    263 rdf:type schema:Organization
    264 grid-institutes:grid.417631.6 schema:alternateName Analytical Chemistry Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India
    265 Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India
    266 Microbial Technology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India
    267 Plant Physiology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India
    268 schema:name Analytical Chemistry Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India
    269 Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India
    270 Microbial Technology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India
    271 Plant Physiology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India
    272 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...