Rho kinase inhibitor enables cell-based therapy for corneal endothelial dysfunction View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-05-18

AUTHORS

Naoki Okumura, Yuji Sakamoto, Keita Fujii, Junji Kitano, Shinichiro Nakano, Yuki Tsujimoto, Shin-ichiro Nakamura, Morio Ueno, Michio Hagiya, Junji Hamuro, Akifumi Matsuyama, Shingo Suzuki, Takashi Shiina, Shigeru Kinoshita, Noriko Koizumi

ABSTRACT

The corneal endothelium maintains corneal transparency; consequently, its dysfunction causes severe vision loss. Tissue engineering-based therapy, as an alternative to conventional donor corneal transplantation, is anticipated to provide a less invasive and more effective therapeutic modality. We conducted a preclinical study for cell-based therapy in a primate model and demonstrated regeneration of the corneal endothelium following injection of cultured monkey corneal endothelial cells (MCECs) or human CECs (HCECs), in combination with a Rho kinase (ROCK) inhibitor, Y-27632, into the anterior chamber. We also evaluated the safety and efficacy of Good Manufacturing Practice (GMP)-grade HCECs, similar to those planned for use as transplant material for human patients in a clinical trial and we showed that the corneal endothelium was regenerated without adverse effect. We also showed that CEC engraftment is impaired by limited substrate adhesion, which is due to actomyosin contraction induced by dissociation-induced activation of ROCK/MLC signaling. Inclusion of a ROCK inhibitor improves efficiency of engraftment of CECs and enables cell-based therapy for treating corneal endothelial dysfunction as a clinically relevant therapy. More... »

PAGES

26113

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep26113

DOI

http://dx.doi.org/10.1038/srep26113

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1039075043

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27189516


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1113", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Ophthalmology and Optometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Transplantation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Corneal Diseases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disease Models, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endothelial Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Macaca fascicularis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Kinase Inhibitors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Treatment Outcome", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "rho-Associated Kinases", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan", 
          "id": "http://www.grid.ac/institutes/grid.255178.c", 
          "name": [
            "Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Okumura", 
        "givenName": "Naoki", 
        "id": "sg:person.0726111404.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0726111404.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Research Laboratory, Senju Pharmaceutical Co., Ltd., Kobe, Japan", 
          "id": "http://www.grid.ac/institutes/grid.480342.9", 
          "name": [
            "Research Laboratory, Senju Pharmaceutical Co., Ltd., Kobe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sakamoto", 
        "givenName": "Yuji", 
        "id": "sg:person.0714270771.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714270771.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan", 
          "id": "http://www.grid.ac/institutes/grid.255178.c", 
          "name": [
            "Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fujii", 
        "givenName": "Keita", 
        "id": "sg:person.01364370713.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01364370713.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan", 
          "id": "http://www.grid.ac/institutes/grid.255178.c", 
          "name": [
            "Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kitano", 
        "givenName": "Junji", 
        "id": "sg:person.0640764613.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640764613.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan", 
          "id": "http://www.grid.ac/institutes/grid.255178.c", 
          "name": [
            "Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nakano", 
        "givenName": "Shinichiro", 
        "id": "sg:person.01065054405.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065054405.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan", 
          "id": "http://www.grid.ac/institutes/grid.255178.c", 
          "name": [
            "Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tsujimoto", 
        "givenName": "Yuki", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Research Center of Animal Life Science, Shiga University of Medical Science, Otsu, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410827.8", 
          "name": [
            "Research Center of Animal Life Science, Shiga University of Medical Science, Otsu, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nakamura", 
        "givenName": "Shin-ichiro", 
        "id": "sg:person.01106026375.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106026375.46"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan", 
          "id": "http://www.grid.ac/institutes/grid.272458.e", 
          "name": [
            "Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ueno", 
        "givenName": "Morio", 
        "id": "sg:person.01074315450.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074315450.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan", 
          "id": "http://www.grid.ac/institutes/grid.272458.e", 
          "name": [
            "Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hagiya", 
        "givenName": "Michio", 
        "id": "sg:person.0725032047.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725032047.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan", 
          "id": "http://www.grid.ac/institutes/grid.272458.e", 
          "name": [
            "Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hamuro", 
        "givenName": "Junji", 
        "id": "sg:person.01236565401.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01236565401.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Platform of Therapeutics for Rare Disease, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Platform of Therapeutics for Rare Disease, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matsuyama", 
        "givenName": "Akifumi", 
        "id": "sg:person.0602331675.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602331675.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Japan", 
          "id": "http://www.grid.ac/institutes/grid.265061.6", 
          "name": [
            "Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Suzuki", 
        "givenName": "Shingo", 
        "id": "sg:person.01137256500.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137256500.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Japan", 
          "id": "http://www.grid.ac/institutes/grid.265061.6", 
          "name": [
            "Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shiina", 
        "givenName": "Takashi", 
        "id": "sg:person.01144706557.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144706557.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan", 
          "id": "http://www.grid.ac/institutes/grid.272458.e", 
          "name": [
            "Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan", 
            "Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kinoshita", 
        "givenName": "Shigeru", 
        "id": "sg:person.01016754101.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016754101.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan", 
          "id": "http://www.grid.ac/institutes/grid.255178.c", 
          "name": [
            "Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Koizumi", 
        "givenName": "Noriko", 
        "id": "sg:person.01351425661.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01351425661.69"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/srep09167", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043554413", 
          "https://doi.org/10.1038/srep09167"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-05-18", 
    "datePublishedReg": "2016-05-18", 
    "description": "The corneal endothelium maintains corneal transparency; consequently, its dysfunction causes severe vision loss. Tissue engineering-based therapy, as an alternative to conventional donor corneal transplantation, is anticipated to provide a less invasive and more effective therapeutic modality. We conducted a preclinical study for cell-based therapy in a primate model and demonstrated regeneration of the corneal endothelium following injection of cultured monkey corneal endothelial cells (MCECs) or human CECs (HCECs), in combination with a Rho kinase (ROCK) inhibitor, Y-27632, into the anterior chamber. We also evaluated the safety and efficacy of Good Manufacturing Practice (GMP)-grade HCECs, similar to those planned for use as transplant material for human patients in a clinical trial and we showed that the corneal endothelium was regenerated without adverse effect. We also showed that CEC engraftment is impaired by limited substrate adhesion, which is due to actomyosin contraction induced by dissociation-induced activation of ROCK/MLC signaling. Inclusion of a ROCK inhibitor improves efficiency of engraftment of CECs and enables cell-based therapy for treating corneal endothelial dysfunction as a clinically relevant therapy.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/srep26113", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.5875707", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.9342073", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.9341907", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "6"
      }
    ], 
    "keywords": [
      "corneal endothelial dysfunction", 
      "monkey corneal endothelial cells", 
      "cell-based therapies", 
      "Rho-kinase inhibitor", 
      "human CECs", 
      "corneal endothelium", 
      "endothelial dysfunction", 
      "kinase inhibitors", 
      "donor corneal transplantation", 
      "severe vision loss", 
      "effective therapeutic modality", 
      "ROCK/MLC", 
      "efficiency of engraftment", 
      "corneal endothelial cells", 
      "anterior chamber", 
      "corneal transplantation", 
      "vision loss", 
      "primate model", 
      "clinical trials", 
      "preclinical studies", 
      "therapeutic modalities", 
      "tissue engineering-based therapies", 
      "human patients", 
      "therapy", 
      "relevant therapies", 
      "corneal transparency", 
      "dysfunction", 
      "endothelial cells", 
      "adverse effects", 
      "endothelium", 
      "transplant material", 
      "engraftment", 
      "ROCK inhibitor", 
      "inhibitors", 
      "good manufacturing practices", 
      "transplantation", 
      "patients", 
      "trials", 
      "efficacy", 
      "modalities", 
      "injection", 
      "contraction", 
      "activation", 
      "safety", 
      "cells", 
      "manufacturing practices", 
      "MLC", 
      "actomyosin contraction", 
      "study", 
      "regeneration", 
      "loss", 
      "effect", 
      "use", 
      "practice", 
      "combination", 
      "adhesion", 
      "inclusion", 
      "alternative", 
      "chamber", 
      "CEC", 
      "model", 
      "substrate adhesion", 
      "materials", 
      "efficiency", 
      "transparency"
    ], 
    "name": "Rho kinase inhibitor enables cell-based therapy for corneal endothelial dysfunction", 
    "pagination": "26113", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1039075043"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/srep26113"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "27189516"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/srep26113", 
      "https://app.dimensions.ai/details/publication/pub.1039075043"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-08-04T17:04", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_704.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/srep26113"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/srep26113'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/srep26113'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/srep26113'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/srep26113'


 

This table displays all metadata directly associated to this object as RDF triples.

293 TRIPLES      21 PREDICATES      102 URIs      93 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/srep26113 schema:about N1f36e3013b4e45029532aa6a07baeca1
2 N258dac628015495bab8193ccbe2fe2eb
3 N3b172357898f429f8b5c25cda500d3fc
4 N5ee16df66aa346bebef910780dc0de32
5 N6d8371013ab548d6af5050617604d24a
6 N753051f119cf4409a0ab565a93ff0fd5
7 N8fdcb95338d9469ca61a44db5c99c084
8 Nba4c8fbb26be4eb39d886c98aacb7fd7
9 Nbc81cae0aef04de895a034d6a46287b7
10 Ne0b51c6ad3194a16945a450540178858
11 Nf50c7f27e9d641c7ab6401bc253245d8
12 anzsrc-for:11
13 anzsrc-for:1113
14 schema:author N4ad2e482680c42f49bbeac3e6e6047fe
15 schema:citation sg:pub.10.1038/srep09167
16 schema:datePublished 2016-05-18
17 schema:datePublishedReg 2016-05-18
18 schema:description The corneal endothelium maintains corneal transparency; consequently, its dysfunction causes severe vision loss. Tissue engineering-based therapy, as an alternative to conventional donor corneal transplantation, is anticipated to provide a less invasive and more effective therapeutic modality. We conducted a preclinical study for cell-based therapy in a primate model and demonstrated regeneration of the corneal endothelium following injection of cultured monkey corneal endothelial cells (MCECs) or human CECs (HCECs), in combination with a Rho kinase (ROCK) inhibitor, Y-27632, into the anterior chamber. We also evaluated the safety and efficacy of Good Manufacturing Practice (GMP)-grade HCECs, similar to those planned for use as transplant material for human patients in a clinical trial and we showed that the corneal endothelium was regenerated without adverse effect. We also showed that CEC engraftment is impaired by limited substrate adhesion, which is due to actomyosin contraction induced by dissociation-induced activation of ROCK/MLC signaling. Inclusion of a ROCK inhibitor improves efficiency of engraftment of CECs and enables cell-based therapy for treating corneal endothelial dysfunction as a clinically relevant therapy.
19 schema:genre article
20 schema:isAccessibleForFree true
21 schema:isPartOf Naf7ec66982a04d78a0b27b08a3c205f1
22 Nc61f618e41a8418eaebb38ee2fb55743
23 sg:journal.1045337
24 schema:keywords CEC
25 MLC
26 ROCK inhibitor
27 ROCK/MLC
28 Rho-kinase inhibitor
29 activation
30 actomyosin contraction
31 adhesion
32 adverse effects
33 alternative
34 anterior chamber
35 cell-based therapies
36 cells
37 chamber
38 clinical trials
39 combination
40 contraction
41 corneal endothelial cells
42 corneal endothelial dysfunction
43 corneal endothelium
44 corneal transparency
45 corneal transplantation
46 donor corneal transplantation
47 dysfunction
48 effect
49 effective therapeutic modality
50 efficacy
51 efficiency
52 efficiency of engraftment
53 endothelial cells
54 endothelial dysfunction
55 endothelium
56 engraftment
57 good manufacturing practices
58 human CECs
59 human patients
60 inclusion
61 inhibitors
62 injection
63 kinase inhibitors
64 loss
65 manufacturing practices
66 materials
67 modalities
68 model
69 monkey corneal endothelial cells
70 patients
71 practice
72 preclinical studies
73 primate model
74 regeneration
75 relevant therapies
76 safety
77 severe vision loss
78 study
79 substrate adhesion
80 therapeutic modalities
81 therapy
82 tissue engineering-based therapies
83 transparency
84 transplant material
85 transplantation
86 trials
87 use
88 vision loss
89 schema:name Rho kinase inhibitor enables cell-based therapy for corneal endothelial dysfunction
90 schema:pagination 26113
91 schema:productId N32c1af976c5b4173989c5d2d4cfdd9ad
92 N6643f245b04042df982a620853f2f3e7
93 N6bfb68e66c3c4eeab069d102f56ce606
94 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039075043
95 https://doi.org/10.1038/srep26113
96 schema:sdDatePublished 2022-08-04T17:04
97 schema:sdLicense https://scigraph.springernature.com/explorer/license/
98 schema:sdPublisher N40f6a381dba24b7da731f01a264a00c9
99 schema:url https://doi.org/10.1038/srep26113
100 sgo:license sg:explorer/license/
101 sgo:sdDataset articles
102 rdf:type schema:ScholarlyArticle
103 N09ee9a4aedaf4c82bf0220de01025928 rdf:first N20769befb0884615a0e9dfed0459e039
104 rdf:rest N623c055791e049de834f18d6e4f3860f
105 N0bb507144843412d970264def9586b3d rdf:first sg:person.01065054405.31
106 rdf:rest N09ee9a4aedaf4c82bf0220de01025928
107 N131c699a7aea4eb4a9072d4cf2fb4ee1 rdf:first sg:person.01236565401.72
108 rdf:rest N6b1ba20389664aa39e61b2cebc088017
109 N1f36e3013b4e45029532aa6a07baeca1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Treatment Outcome
111 rdf:type schema:DefinedTerm
112 N20769befb0884615a0e9dfed0459e039 schema:affiliation grid-institutes:grid.255178.c
113 schema:familyName Tsujimoto
114 schema:givenName Yuki
115 rdf:type schema:Person
116 N258dac628015495bab8193ccbe2fe2eb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Cells, Cultured
118 rdf:type schema:DefinedTerm
119 N32c1af976c5b4173989c5d2d4cfdd9ad schema:name pubmed_id
120 schema:value 27189516
121 rdf:type schema:PropertyValue
122 N3a73e6473bbb4d41a60fb416de7b76ed rdf:first sg:person.01016754101.20
123 rdf:rest Nae0ef82a1aeb44efa715fb383e5463f9
124 N3b172357898f429f8b5c25cda500d3fc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Corneal Diseases
126 rdf:type schema:DefinedTerm
127 N40f6a381dba24b7da731f01a264a00c9 schema:name Springer Nature - SN SciGraph project
128 rdf:type schema:Organization
129 N4ad2e482680c42f49bbeac3e6e6047fe rdf:first sg:person.0726111404.15
130 rdf:rest N8e8be12ac69c47a8b392d49624787cd4
131 N5622f44faf0f4a15af5fc11461442342 rdf:first sg:person.01074315450.48
132 rdf:rest Ndca8b42f6c41451a8e4ada99bed3f29b
133 N5ee16df66aa346bebef910780dc0de32 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Cell Transplantation
135 rdf:type schema:DefinedTerm
136 N623c055791e049de834f18d6e4f3860f rdf:first sg:person.01106026375.46
137 rdf:rest N5622f44faf0f4a15af5fc11461442342
138 N6643f245b04042df982a620853f2f3e7 schema:name doi
139 schema:value 10.1038/srep26113
140 rdf:type schema:PropertyValue
141 N6b1ba20389664aa39e61b2cebc088017 rdf:first sg:person.0602331675.13
142 rdf:rest Neb1cfacdb0164d84818e667828136337
143 N6bfb68e66c3c4eeab069d102f56ce606 schema:name dimensions_id
144 schema:value pub.1039075043
145 rdf:type schema:PropertyValue
146 N6d8371013ab548d6af5050617604d24a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Endothelial Cells
148 rdf:type schema:DefinedTerm
149 N753051f119cf4409a0ab565a93ff0fd5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Animals
151 rdf:type schema:DefinedTerm
152 N8e8be12ac69c47a8b392d49624787cd4 rdf:first sg:person.0714270771.62
153 rdf:rest Nf31a57932bd64ba1b769bf2ed25a8009
154 N8fdcb95338d9469ca61a44db5c99c084 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Macaca fascicularis
156 rdf:type schema:DefinedTerm
157 N928ea5c787344b5e9be73a7d5112bb40 rdf:first sg:person.0640764613.03
158 rdf:rest N0bb507144843412d970264def9586b3d
159 Nae0ef82a1aeb44efa715fb383e5463f9 rdf:first sg:person.01351425661.69
160 rdf:rest rdf:nil
161 Naf7ec66982a04d78a0b27b08a3c205f1 schema:volumeNumber 6
162 rdf:type schema:PublicationVolume
163 Nba4c8fbb26be4eb39d886c98aacb7fd7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name rho-Associated Kinases
165 rdf:type schema:DefinedTerm
166 Nbc81cae0aef04de895a034d6a46287b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Protein Kinase Inhibitors
168 rdf:type schema:DefinedTerm
169 Nc61f618e41a8418eaebb38ee2fb55743 schema:issueNumber 1
170 rdf:type schema:PublicationIssue
171 Ndca8b42f6c41451a8e4ada99bed3f29b rdf:first sg:person.0725032047.17
172 rdf:rest N131c699a7aea4eb4a9072d4cf2fb4ee1
173 Ne0b51c6ad3194a16945a450540178858 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
174 schema:name Disease Models, Animal
175 rdf:type schema:DefinedTerm
176 Neb1cfacdb0164d84818e667828136337 rdf:first sg:person.01137256500.26
177 rdf:rest Nec857b6e72c54b41b0dee005ae527393
178 Nec857b6e72c54b41b0dee005ae527393 rdf:first sg:person.01144706557.29
179 rdf:rest N3a73e6473bbb4d41a60fb416de7b76ed
180 Nf31a57932bd64ba1b769bf2ed25a8009 rdf:first sg:person.01364370713.09
181 rdf:rest N928ea5c787344b5e9be73a7d5112bb40
182 Nf50c7f27e9d641c7ab6401bc253245d8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
183 schema:name Humans
184 rdf:type schema:DefinedTerm
185 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
186 schema:name Medical and Health Sciences
187 rdf:type schema:DefinedTerm
188 anzsrc-for:1113 schema:inDefinedTermSet anzsrc-for:
189 schema:name Ophthalmology and Optometry
190 rdf:type schema:DefinedTerm
191 sg:grant.5875707 http://pending.schema.org/fundedItem sg:pub.10.1038/srep26113
192 rdf:type schema:MonetaryGrant
193 sg:grant.9341907 http://pending.schema.org/fundedItem sg:pub.10.1038/srep26113
194 rdf:type schema:MonetaryGrant
195 sg:grant.9342073 http://pending.schema.org/fundedItem sg:pub.10.1038/srep26113
196 rdf:type schema:MonetaryGrant
197 sg:journal.1045337 schema:issn 2045-2322
198 schema:name Scientific Reports
199 schema:publisher Springer Nature
200 rdf:type schema:Periodical
201 sg:person.01016754101.20 schema:affiliation grid-institutes:grid.272458.e
202 schema:familyName Kinoshita
203 schema:givenName Shigeru
204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016754101.20
205 rdf:type schema:Person
206 sg:person.01065054405.31 schema:affiliation grid-institutes:grid.255178.c
207 schema:familyName Nakano
208 schema:givenName Shinichiro
209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065054405.31
210 rdf:type schema:Person
211 sg:person.01074315450.48 schema:affiliation grid-institutes:grid.272458.e
212 schema:familyName Ueno
213 schema:givenName Morio
214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074315450.48
215 rdf:type schema:Person
216 sg:person.01106026375.46 schema:affiliation grid-institutes:grid.410827.8
217 schema:familyName Nakamura
218 schema:givenName Shin-ichiro
219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106026375.46
220 rdf:type schema:Person
221 sg:person.01137256500.26 schema:affiliation grid-institutes:grid.265061.6
222 schema:familyName Suzuki
223 schema:givenName Shingo
224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137256500.26
225 rdf:type schema:Person
226 sg:person.01144706557.29 schema:affiliation grid-institutes:grid.265061.6
227 schema:familyName Shiina
228 schema:givenName Takashi
229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144706557.29
230 rdf:type schema:Person
231 sg:person.01236565401.72 schema:affiliation grid-institutes:grid.272458.e
232 schema:familyName Hamuro
233 schema:givenName Junji
234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01236565401.72
235 rdf:type schema:Person
236 sg:person.01351425661.69 schema:affiliation grid-institutes:grid.255178.c
237 schema:familyName Koizumi
238 schema:givenName Noriko
239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01351425661.69
240 rdf:type schema:Person
241 sg:person.01364370713.09 schema:affiliation grid-institutes:grid.255178.c
242 schema:familyName Fujii
243 schema:givenName Keita
244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01364370713.09
245 rdf:type schema:Person
246 sg:person.0602331675.13 schema:affiliation grid-institutes:None
247 schema:familyName Matsuyama
248 schema:givenName Akifumi
249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602331675.13
250 rdf:type schema:Person
251 sg:person.0640764613.03 schema:affiliation grid-institutes:grid.255178.c
252 schema:familyName Kitano
253 schema:givenName Junji
254 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0640764613.03
255 rdf:type schema:Person
256 sg:person.0714270771.62 schema:affiliation grid-institutes:grid.480342.9
257 schema:familyName Sakamoto
258 schema:givenName Yuji
259 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714270771.62
260 rdf:type schema:Person
261 sg:person.0725032047.17 schema:affiliation grid-institutes:grid.272458.e
262 schema:familyName Hagiya
263 schema:givenName Michio
264 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725032047.17
265 rdf:type schema:Person
266 sg:person.0726111404.15 schema:affiliation grid-institutes:grid.255178.c
267 schema:familyName Okumura
268 schema:givenName Naoki
269 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0726111404.15
270 rdf:type schema:Person
271 sg:pub.10.1038/srep09167 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043554413
272 https://doi.org/10.1038/srep09167
273 rdf:type schema:CreativeWork
274 grid-institutes:None schema:alternateName Platform of Therapeutics for Rare Disease, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan
275 schema:name Platform of Therapeutics for Rare Disease, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan
276 rdf:type schema:Organization
277 grid-institutes:grid.255178.c schema:alternateName Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan
278 schema:name Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan
279 rdf:type schema:Organization
280 grid-institutes:grid.265061.6 schema:alternateName Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Japan
281 schema:name Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Japan
282 rdf:type schema:Organization
283 grid-institutes:grid.272458.e schema:alternateName Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
284 Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
285 schema:name Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
286 Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
287 rdf:type schema:Organization
288 grid-institutes:grid.410827.8 schema:alternateName Research Center of Animal Life Science, Shiga University of Medical Science, Otsu, Japan
289 schema:name Research Center of Animal Life Science, Shiga University of Medical Science, Otsu, Japan
290 rdf:type schema:Organization
291 grid-institutes:grid.480342.9 schema:alternateName Research Laboratory, Senju Pharmaceutical Co., Ltd., Kobe, Japan
292 schema:name Research Laboratory, Senju Pharmaceutical Co., Ltd., Kobe, Japan
293 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...