The CagA toxin of Helicobacter pylori: abundant production but relatively low amount translocated View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-03-17

AUTHORS

Luisa F. Jiménez-Soto, Rainer Haas

ABSTRACT

CagA is one of the most studied pathogenicity factors of the bacterial pathogen Helicobacter pylori. It is injected into host cells via the H. pylori cag-Type IV secretion system. Due to its association with gastric cancer, CagA is classified as oncogenic protein. At the same time CagA represents the 4th most abundant protein produced by H. pylori, suggesting that high amounts of toxin are required to cause the physiological changes or damage observed in cells. We were able to quantify the injection of CagA into gastric AGS epithelial cells in vitro by the adaptation of a novel protease-based approach to remove the tightly adherent extracellular bacteria. After one hour of infection only 1.5% of the total CagA available was injected by the adherent bacteria, whereas after 3 hours 7.5% was found within the host cell. Thus, our data show that only a surprisingly small amount of the CagA available in the infection is finally injected under in vitro infection conditions. More... »

PAGES

23227

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep23227

DOI

http://dx.doi.org/10.1038/srep23227

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019904918

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26983895


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