nagZ Triggers Gonococcal Biofilm Disassembly View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-04

AUTHORS

Senthil V Bhoopalan, Andrzej Piekarowicz, Jonathan D Lenz, Joseph P Dillard, Daniel C Stein

ABSTRACT

Bacterial-bacterial interactions play a critical role in promoting biofilm formation. Here we show that NagZ, a protein associated with peptidoglycan recycling, has moonlighting activity that allows it to modulate biofilm accumulation by Neisseria gonorrhoeae. We characterize the biochemical properties of NagZ and demonstrate its ability to function as a dispersing agent for biofilms formed on abiotic surfaces. We extend these observations to cell culture and tissue explant models and show that in nagZ mutants, the biofilms formed in cell culture and on human tissues contain significantly more biomass than those formed by a wild-type strain. Our results demonstrate that an enzyme thought to be restricted to peptidoglycan recycling is able to disperse preformed biofilms. More... »

PAGES

22372

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep22372

DOI

http://dx.doi.org/10.1038/srep22372

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043695785

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26927542


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