Exploring miniature insect brains using micro-CT scanning techniques View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-04

AUTHORS

Dylan B Smith, Galina Bernhardt, Nigel E Raine, Richard L Abel, Dan Sykes, Farah Ahmed, Inti Pedroso, Richard J Gill

ABSTRACT

The capacity to explore soft tissue structures in detail is important in understanding animal physiology and how this determines features such as movement, behaviour and the impact of trauma on regular function. Here we use advances in micro-computed tomography (micro-CT) technology to explore the brain of an important insect pollinator and model organism, the bumblebee (Bombus terrestris). Here we present a method for accurate imaging and exploration of insect brains that keeps brain tissue free from trauma and in its natural stereo-geometry, and showcase our 3D reconstructions and analyses of 19 individual brains at high resolution. Development of this protocol allows relatively rapid and cost effective brain reconstructions, making it an accessible methodology to the wider scientific community. The protocol describes the necessary steps for sample preparation, tissue staining, micro-CT scanning and 3D reconstruction, followed by a method for image analysis using the freeware SPIERS. These image analysis methods describe how to virtually extract key composite structures from the insect brain, and we demonstrate the application and precision of this method by calculating structural volumes and investigating the allometric relationships between bumblebee brain structures. More... »

PAGES

21768

References to SciGraph publications

  • 2009-07. Olfactory learning and memory in the bumblebee Bombus occidentalis in THE SCIENCE OF NATURE
  • 2006-12. Fast-Find: A novel computational approach to analyzing combinatorial motifs in BMC BIOINFORMATICS
  • 2002-07. Memory use in insect visual navigation in NATURE REVIEWS NEUROSCIENCE
  • 2001-04. The concepts of ‘sameness’ and ‘difference’ in an insect in NATURE
  • 2013-07-17. Neuroscience: Solving the brain in NATURE
  • 1996-08. Symmetry perception in an insect in NATURE
  • 2009-12. MicroCT for comparative morphology: simple staining methods allow high-contrast 3D imaging of diverse non-mineralized animal tissues in BMC PHYSIOLOGY
  • 2010-07. 3D visualization of the microstructure of Quedius beesoni Cameron using micro-CT in ANALYTICAL AND BIOANALYTICAL CHEMISTRY
  • 1999-02. A digital three-dimensional atlas of the honeybee antennal lobe based on optical sections acquired by confocal microscopy in CELL AND TISSUE RESEARCH
  • 2012-11. The honeybee as a model for understanding the basis of cognition in NATURE REVIEWS NEUROSCIENCE
  • 2008-10. Evidence for counting in insects in ANIMAL COGNITION
  • 2015-12. Bumblebee learning and memory is impaired by chronic exposure to a neonicotinoid pesticide in SCIENTIFIC REPORTS
  • 2003-08. Desert ant navigation: how miniature brains solve complex tasks in JOURNAL OF COMPARATIVE PHYSIOLOGY A
  • 2006-12. The Virtual Insect Brain protocol: creating and comparing standardized neuroanatomy in BMC BIOINFORMATICS
  • 2013-05. Inter-tegular span and head width as estimators of fresh and dry body mass in bumblebees (Bombus spp.) in INSECTES SOCIAUX
  • 2007-05. Noninvasive detection of macrophages using a nanoparticulate contrast agent for computed tomography in NATURE MEDICINE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/srep21768

    DOI

    http://dx.doi.org/10.1038/srep21768

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1023767045

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26908205


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