CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-04

AUTHORS

Shu Su, Bian Hu, Jie Shao, Bin Shen, Juan Du, Yinan Du, Jiankui Zhou, Lixia Yu, Lianru Zhang, Fangjun Chen, Huizi Sha, Lei Cheng, Fanyan Meng, Zhengyun Zou, Xingxu Huang, Baorui Liu

ABSTRACT

Strategies that enhance the function of T cells are critical for immunotherapy. One negative regulator of T-cell activity is ligand PD-L1, which is expressed on dentritic cells (DCs) or some tumor cells, and functions through binding of programmed death-1 (PD-1) receptor on activated T cells. Here we described for the first time a non-viral mediated approach to reprogram primary human T cells by disruption of PD-1. We showed that the gene knockout of PD-1 by electroporation of plasmids encoding sgRNA and Cas9 was technically feasible. The disruption of inhibitory checkpoint gene PD-1 resulted in significant reduction of PD-1 expression but didn't affect the viability of primary human T cells during the prolonged in vitro culture. Cellular immune response of the gene modified T cells was characterized by up-regulated IFN-γ production and enhanced cytotoxicity. These results suggest that we have demonstrated an approach for efficient checkpoint inhibitor disruption in T cells, providing a new strategy for targeting checkpoint inhibitors, which could potentialy be useful to improve the efficacy of T-cell based adoptive therapies. More... »

PAGES

20070

References to SciGraph publications

  • 2013-08. Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems in NATURE BIOTECHNOLOGY
  • 2012-10. siRNA-mediated silencing of PD-1 ligands enhances tumor-specific human T-cell effector functions in GENE THERAPY
  • 2014-11. PD-1 blockade induces responses by inhibiting adaptive immune resistance in NATURE
  • 2010-08. RNA interference targeting programmed death receptor-1 improves immune functions of tumor-specific T cells in CANCER IMMUNOLOGY, IMMUNOTHERAPY
  • 2014-03. Interference with PD-L1/PD-1 co-stimulation during antigen presentation enhances the multifunctionality of antigen-specific T cells in GENE THERAPY
  • 2003-11. Ex vivo identification, isolation and analysis of tumor-cytolytic T cells in NATURE MEDICINE
  • 2005-02. Recent developments in therapeutic cancer vaccines in NATURE REVIEWS CLINICAL ONCOLOGY
  • 2013-05. Generation of gene-modified mice via Cas9/RNA-mediated gene targeting in CELL RESEARCH
  • 2001-10. Regulation of tumor growth by IFN-γ in cancer immunotherapy in IMMUNOLOGIC RESEARCH
  • 2013-04. Persistent antigen at vaccination sites induces tumor-specific CD8+ T cell sequestration, dysfunction and deletion in NATURE MEDICINE
  • 2004-09. Cancer immunotherapy: moving beyond current vaccines in NATURE MEDICINE
  • 2013-10. Cas9 as a versatile tool for engineering biology in NATURE METHODS
  • 2014-01. Generating rats with conditional alleles using CRISPR/Cas9 in CELL RESEARCH
  • 2014-04. Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects in NATURE METHODS
  • 2012-04. The blockade of immune checkpoints in cancer immunotherapy in NATURE REVIEWS CANCER
  • 2012-05. Editing T cell specificity towards leukemia by zinc finger nucleases and lentiviral gene transfer in NATURE MEDICINE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/srep20070

    DOI

    http://dx.doi.org/10.1038/srep20070

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024675113

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26818188


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Immunology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "CRISPR-Cas Systems", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Cultured", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cytokines", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cytotoxicity, Immunologic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Editing", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Knockout Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Order", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Targeting", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Transfer Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Loci", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Vectors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Lymphocyte Activation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Neoplasms", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Phenotype", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Programmed Cell Death 1 Receptor", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA, Guide", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "T-Lymphocyte Subsets", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transfection", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Nanjing Drum Tower Hospital", 
              "id": "https://www.grid.ac/institutes/grid.428392.6", 
              "name": [
                "The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Su", 
            "givenName": "Shu", 
            "id": "sg:person.01335571301.60", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335571301.60"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Model Animal Research Center", 
              "id": "https://www.grid.ac/institutes/grid.452564.4", 
              "name": [
                "MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, National Resource Center for Mutant Mice, Nanjing 210061, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hu", 
            "givenName": "Bian", 
            "id": "sg:person.0714167670.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714167670.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Nanjing Drum Tower Hospital", 
              "id": "https://www.grid.ac/institutes/grid.428392.6", 
              "name": [
                "The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shao", 
            "givenName": "Jie", 
            "id": "sg:person.0660300401.82", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0660300401.82"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Nanjing Medical University", 
              "id": "https://www.grid.ac/institutes/grid.89957.3a", 
              "name": [
                "State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shen", 
            "givenName": "Bin", 
            "id": "sg:person.0674155231.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0674155231.70"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Nanjing Drum Tower Hospital", 
              "id": "https://www.grid.ac/institutes/grid.428392.6", 
              "name": [
                "The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Du", 
            "givenName": "Juan", 
            "id": "sg:person.0774527001.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0774527001.50"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Model Animal Research Center", 
              "id": "https://www.grid.ac/institutes/grid.452564.4", 
              "name": [
                "MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, National Resource Center for Mutant Mice, Nanjing 210061, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Du", 
            "givenName": "Yinan", 
            "id": "sg:person.01010403631.18", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010403631.18"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Model Animal Research Center", 
              "id": "https://www.grid.ac/institutes/grid.452564.4", 
              "name": [
                "MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, National Resource Center for Mutant Mice, Nanjing 210061, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhou", 
            "givenName": "Jiankui", 
            "id": "sg:person.01024251730.25", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01024251730.25"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Nanjing Drum Tower Hospital", 
              "id": "https://www.grid.ac/institutes/grid.428392.6", 
              "name": [
                "The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yu", 
            "givenName": "Lixia", 
            "id": "sg:person.0715553130.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715553130.19"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Nanjing Drum Tower Hospital", 
              "id": "https://www.grid.ac/institutes/grid.428392.6", 
              "name": [
                "The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Lianru", 
            "id": "sg:person.01225204001.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01225204001.22"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Nanjing Drum Tower Hospital", 
              "id": "https://www.grid.ac/institutes/grid.428392.6", 
              "name": [
                "The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chen", 
            "givenName": "Fangjun", 
            "id": "sg:person.01273317201.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01273317201.05"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Nanjing Drum Tower Hospital", 
              "id": "https://www.grid.ac/institutes/grid.428392.6", 
              "name": [
                "The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sha", 
            "givenName": "Huizi", 
            "id": "sg:person.01322162704.14", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322162704.14"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Nanjing Drum Tower Hospital", 
              "id": "https://www.grid.ac/institutes/grid.428392.6", 
              "name": [
                "The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Cheng", 
            "givenName": "Lei", 
            "id": "sg:person.0616026301.65", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616026301.65"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Nanjing Drum Tower Hospital", 
              "id": "https://www.grid.ac/institutes/grid.428392.6", 
              "name": [
                "The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Meng", 
            "givenName": "Fanyan", 
            "id": "sg:person.01163153766.89", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163153766.89"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Nanjing Drum Tower Hospital", 
              "id": "https://www.grid.ac/institutes/grid.428392.6", 
              "name": [
                "The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zou", 
            "givenName": "Zhengyun", 
            "id": "sg:person.01045237730.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01045237730.27"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "ShanghaiTech University", 
              "id": "https://www.grid.ac/institutes/grid.440637.2", 
              "name": [
                "MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, National Resource Center for Mutant Mice, Nanjing 210061, China", 
                "School of Life Science and Technology, ShanghaiTech University, 100 Haike Rd., Pudong New Area, Shanghai 201210, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Huang", 
            "givenName": "Xingxu", 
            "id": "sg:person.0730336324.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0730336324.63"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Nanjing Drum Tower Hospital", 
              "id": "https://www.grid.ac/institutes/grid.428392.6", 
              "name": [
                "The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Liu", 
            "givenName": "Baorui", 
            "id": "sg:person.01232700601.24", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01232700601.24"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1158/0008-5472.can-14-1258", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001080038"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/0008-5472.can-14-1258", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001080038"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.18632/oncotarget.2980", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001783473"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm1100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001965372", 
              "https://doi.org/10.1038/nm1100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm1100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001965372", 
              "https://doi.org/10.1038/nm1100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.immunol.26.021607.090331", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005008646"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm.3105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008050192", 
              "https://doi.org/10.1038/nm.3105"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm942", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008593772", 
              "https://doi.org/10.1038/nm942"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm942", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008593772", 
              "https://doi.org/10.1038/nm942"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1385/ir:24:2:201", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008620959", 
              "https://doi.org/10.1385/ir:24:2:201"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2649", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008703730", 
              "https://doi.org/10.1038/nmeth.2649"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.7555/jbr.28.20140066", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013110661"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/intimm/dxm091", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015532387"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/gt.2013.80", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017529500", 
              "https://doi.org/10.1038/gt.2013.80"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/gt.2013.80", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017529500", 
              "https://doi.org/10.1038/gt.2013.80"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2013.157", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017624741", 
              "https://doi.org/10.1038/cr.2013.157"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0064138", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017756173"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pmed.0010028", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018097004"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature13954", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020455470", 
              "https://doi.org/10.1038/nature13954"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/ijc.21775", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022291558"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/ijc.21775", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022291558"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncponc0098", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026358991", 
              "https://doi.org/10.1038/ncponc0098"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncponc0098", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026358991", 
              "https://doi.org/10.1038/ncponc0098"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/gt.2011.185", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029720470", 
              "https://doi.org/10.1038/gt.2011.185"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2857", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035757498", 
              "https://doi.org/10.1038/nmeth.2857"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3389/fimmu.2013.00221", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038614191"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/cr.2013.46", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038811201", 
              "https://doi.org/10.1038/cr.2013.46"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0060298", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040501714"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00262-010-0842-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041143302", 
              "https://doi.org/10.1007/s00262-010-0842-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00262-010-0842-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041143302", 
              "https://doi.org/10.1007/s00262-010-0842-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1100213108", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041154581"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1225829", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041850060"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1056/nejmoa1200690", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042896583"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/01.cji.0000162780.96310.e4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044545822"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/01.cji.0000162780.96310.e4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044545822"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/01.cji.0000162780.96310.e4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044545822"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2014.01.027", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046173829"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/1078-0432.ccr-13-0458", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046840369"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/1078-0432.ccr-07-4079", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047225303"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gks1446", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047402217"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1056/nejmoa1200694", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047413837"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc3239", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047946992", 
              "https://doi.org/10.1038/nrc3239"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2652", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048853216", 
              "https://doi.org/10.1038/nbt.2652"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm.2700", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050105684", 
              "https://doi.org/10.1038/nm.2700"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/intimm/dxp072", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051487407"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2016-04", 
        "datePublishedReg": "2016-04-01", 
        "description": "Strategies that enhance the function of T cells are critical for immunotherapy. One negative regulator of T-cell activity is ligand PD-L1, which is expressed on dentritic cells (DCs) or some tumor cells, and functions through binding of programmed death-1 (PD-1) receptor on activated T cells. Here we described for the first time a non-viral mediated approach to reprogram primary human T cells by disruption of PD-1. We showed that the gene knockout of PD-1 by electroporation of plasmids encoding sgRNA and Cas9 was technically feasible. The disruption of inhibitory checkpoint gene PD-1 resulted in significant reduction of PD-1 expression but didn't affect the viability of primary human T cells during the prolonged in vitro culture. Cellular immune response of the gene modified T cells was characterized by up-regulated IFN-\u03b3 production and enhanced cytotoxicity. These results suggest that we have demonstrated an approach for efficient checkpoint inhibitor disruption in T cells, providing a new strategy for targeting checkpoint inhibitors, which could potentialy be useful to improve the efficacy of T-cell based adoptive therapies. ", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/srep20070", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.7023314", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.6993090", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.5008637", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1045337", 
            "issn": [
              "2045-2322"
            ], 
            "name": "Scientific Reports", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "6"
          }
        ], 
        "name": "CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients", 
        "pagination": "20070", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "758d912c4e6d2de905a726dbfe7d0fa82837e727bd7439e79da28965256b1725"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "26818188"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101563288"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/srep20070"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1024675113"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/srep20070", 
          "https://app.dimensions.ai/details/publication/pub.1024675113"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T20:53", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8684_00000550.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/srep20070"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/srep20070'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/srep20070'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/srep20070'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/srep20070'


     

    This table displays all metadata directly associated to this object as RDF triples.

    393 TRIPLES      21 PREDICATES      85 URIs      41 LITERALS      29 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/srep20070 schema:about N05730a16f1404a48b431681e9e9d542d
    2 N0a11c411286a4ffb9c87ebdfb2d3f47a
    3 N0b2bd466893045cf8643e433438d4af3
    4 N0ca8e2c848954372b1334839583582a2
    5 N21f03633271c4f6b980b106f473d3d25
    6 N327176a79c9d480aafe335878e91db9a
    7 N4385d1fd7c6843e099b63f8022443cf4
    8 N47d5ad89c7454d3bb1a37dc4acf6f1ff
    9 N5384420a983744458c63661a0d2216a4
    10 N808864eea685412bb537869253197493
    11 N818b5b75694a44ad8a179f545c63ab4a
    12 N916da39f08634e6fa8fa409efb123df3
    13 N9790e51124ad4d6da2aa589d9708a606
    14 N9b8693c0fde1495c8ce0b7687451c1ab
    15 Nabf90713426a47fd96bf9d272e504a02
    16 Nb9fc69a7f29b40ebb1b19c04c979b39c
    17 Nbf59366f196a47b19cddca3857eadf4d
    18 Nc7c2d2e26b2748c7ab9e2e78d79f2ee6
    19 Nc7d3fd32562d4abe862e8ab4616e0786
    20 Nd3572d088fc94f788dd51ba9f0fe0aa3
    21 anzsrc-for:11
    22 anzsrc-for:1107
    23 schema:author N8a9eaf3a6d804315ac51c179dec83d59
    24 schema:citation sg:pub.10.1007/s00262-010-0842-0
    25 sg:pub.10.1038/cr.2013.157
    26 sg:pub.10.1038/cr.2013.46
    27 sg:pub.10.1038/gt.2011.185
    28 sg:pub.10.1038/gt.2013.80
    29 sg:pub.10.1038/nature13954
    30 sg:pub.10.1038/nbt.2652
    31 sg:pub.10.1038/ncponc0098
    32 sg:pub.10.1038/nm.2700
    33 sg:pub.10.1038/nm.3105
    34 sg:pub.10.1038/nm1100
    35 sg:pub.10.1038/nm942
    36 sg:pub.10.1038/nmeth.2649
    37 sg:pub.10.1038/nmeth.2857
    38 sg:pub.10.1038/nrc3239
    39 sg:pub.10.1385/ir:24:2:201
    40 https://doi.org/10.1002/ijc.21775
    41 https://doi.org/10.1016/j.cell.2014.01.027
    42 https://doi.org/10.1056/nejmoa1200690
    43 https://doi.org/10.1056/nejmoa1200694
    44 https://doi.org/10.1073/pnas.1100213108
    45 https://doi.org/10.1093/intimm/dxm091
    46 https://doi.org/10.1093/intimm/dxp072
    47 https://doi.org/10.1093/nar/gks1446
    48 https://doi.org/10.1097/01.cji.0000162780.96310.e4
    49 https://doi.org/10.1126/science.1225829
    50 https://doi.org/10.1146/annurev.immunol.26.021607.090331
    51 https://doi.org/10.1158/0008-5472.can-14-1258
    52 https://doi.org/10.1158/1078-0432.ccr-07-4079
    53 https://doi.org/10.1158/1078-0432.ccr-13-0458
    54 https://doi.org/10.1371/journal.pmed.0010028
    55 https://doi.org/10.1371/journal.pone.0060298
    56 https://doi.org/10.1371/journal.pone.0064138
    57 https://doi.org/10.18632/oncotarget.2980
    58 https://doi.org/10.3389/fimmu.2013.00221
    59 https://doi.org/10.7555/jbr.28.20140066
    60 schema:datePublished 2016-04
    61 schema:datePublishedReg 2016-04-01
    62 schema:description Strategies that enhance the function of T cells are critical for immunotherapy. One negative regulator of T-cell activity is ligand PD-L1, which is expressed on dentritic cells (DCs) or some tumor cells, and functions through binding of programmed death-1 (PD-1) receptor on activated T cells. Here we described for the first time a non-viral mediated approach to reprogram primary human T cells by disruption of PD-1. We showed that the gene knockout of PD-1 by electroporation of plasmids encoding sgRNA and Cas9 was technically feasible. The disruption of inhibitory checkpoint gene PD-1 resulted in significant reduction of PD-1 expression but didn't affect the viability of primary human T cells during the prolonged in vitro culture. Cellular immune response of the gene modified T cells was characterized by up-regulated IFN-γ production and enhanced cytotoxicity. These results suggest that we have demonstrated an approach for efficient checkpoint inhibitor disruption in T cells, providing a new strategy for targeting checkpoint inhibitors, which could potentialy be useful to improve the efficacy of T-cell based adoptive therapies.
    63 schema:genre research_article
    64 schema:inLanguage en
    65 schema:isAccessibleForFree true
    66 schema:isPartOf N54d37da35b2745209393ed5b67c3dc96
    67 Ncacaf2f484dd457c88f46065939995a2
    68 sg:journal.1045337
    69 schema:name CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients
    70 schema:pagination 20070
    71 schema:productId N140ff897309b45e48faf9ccbb8b7d83e
    72 N3aad959bfa4b43fd8c8809ddcce21c67
    73 N6ce8190bc9dc446eada9d70f1424dfbe
    74 Nb7c800864b394498978ad8d04a8806d1
    75 Nd737c4746483458da9c8528ebf773029
    76 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024675113
    77 https://doi.org/10.1038/srep20070
    78 schema:sdDatePublished 2019-04-10T20:53
    79 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    80 schema:sdPublisher N90831e27b819422da08f7ce3cef77836
    81 schema:url http://www.nature.com/articles/srep20070
    82 sgo:license sg:explorer/license/
    83 sgo:sdDataset articles
    84 rdf:type schema:ScholarlyArticle
    85 N05730a16f1404a48b431681e9e9d542d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    86 schema:name Humans
    87 rdf:type schema:DefinedTerm
    88 N0a11c411286a4ffb9c87ebdfb2d3f47a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    89 schema:name Gene Order
    90 rdf:type schema:DefinedTerm
    91 N0b2bd466893045cf8643e433438d4af3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    92 schema:name CRISPR-Cas Systems
    93 rdf:type schema:DefinedTerm
    94 N0ca8e2c848954372b1334839583582a2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    95 schema:name Lymphocyte Activation
    96 rdf:type schema:DefinedTerm
    97 N140ff897309b45e48faf9ccbb8b7d83e schema:name doi
    98 schema:value 10.1038/srep20070
    99 rdf:type schema:PropertyValue
    100 N1a57d4f8427a4bed8072c1bd8298f9de rdf:first sg:person.01163153766.89
    101 rdf:rest Na5972ba8ca7243579cd40a15afb53175
    102 N1b9c8cbeb9e0441289ed31e6daf6e51e rdf:first sg:person.0715553130.19
    103 rdf:rest Nb47f081a7a624fe1b734c8401d4b220b
    104 N21f03633271c4f6b980b106f473d3d25 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Programmed Cell Death 1 Receptor
    106 rdf:type schema:DefinedTerm
    107 N300dadea62f9482ab0a1f2eb0d88b092 rdf:first sg:person.01024251730.25
    108 rdf:rest N1b9c8cbeb9e0441289ed31e6daf6e51e
    109 N327176a79c9d480aafe335878e91db9a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Cytotoxicity, Immunologic
    111 rdf:type schema:DefinedTerm
    112 N383dcbebf0964b8e8ff30c64efdb6ec8 rdf:first sg:person.0730336324.63
    113 rdf:rest N74793c90c5df4bb6bd5b98c2f50a0f46
    114 N3aad959bfa4b43fd8c8809ddcce21c67 schema:name dimensions_id
    115 schema:value pub.1024675113
    116 rdf:type schema:PropertyValue
    117 N4385d1fd7c6843e099b63f8022443cf4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name Phenotype
    119 rdf:type schema:DefinedTerm
    120 N47d5ad89c7454d3bb1a37dc4acf6f1ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name Cells, Cultured
    122 rdf:type schema:DefinedTerm
    123 N5384420a983744458c63661a0d2216a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    124 schema:name Base Sequence
    125 rdf:type schema:DefinedTerm
    126 N54d37da35b2745209393ed5b67c3dc96 schema:issueNumber 1
    127 rdf:type schema:PublicationIssue
    128 N557eac1b6e9e4794972418831439e42c rdf:first sg:person.0714167670.35
    129 rdf:rest Nc30df37fe2e740bba74597fabdac154a
    130 N6ce8190bc9dc446eada9d70f1424dfbe schema:name readcube_id
    131 schema:value 758d912c4e6d2de905a726dbfe7d0fa82837e727bd7439e79da28965256b1725
    132 rdf:type schema:PropertyValue
    133 N74793c90c5df4bb6bd5b98c2f50a0f46 rdf:first sg:person.01232700601.24
    134 rdf:rest rdf:nil
    135 N77cea6cc7e664f48a527a7b468645cd1 rdf:first sg:person.0774527001.50
    136 rdf:rest Nc6fd0630b313412594752c73228b1b1c
    137 N7e24d55d38434e4bbe8afa84ead2f933 rdf:first sg:person.0674155231.70
    138 rdf:rest N77cea6cc7e664f48a527a7b468645cd1
    139 N808864eea685412bb537869253197493 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    140 schema:name Genetic Loci
    141 rdf:type schema:DefinedTerm
    142 N818b5b75694a44ad8a179f545c63ab4a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    143 schema:name Gene Targeting
    144 rdf:type schema:DefinedTerm
    145 N8a9eaf3a6d804315ac51c179dec83d59 rdf:first sg:person.01335571301.60
    146 rdf:rest N557eac1b6e9e4794972418831439e42c
    147 N90831e27b819422da08f7ce3cef77836 schema:name Springer Nature - SN SciGraph project
    148 rdf:type schema:Organization
    149 N916da39f08634e6fa8fa409efb123df3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name T-Lymphocyte Subsets
    151 rdf:type schema:DefinedTerm
    152 N9790e51124ad4d6da2aa589d9708a606 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Cytokines
    154 rdf:type schema:DefinedTerm
    155 N9b8693c0fde1495c8ce0b7687451c1ab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name Neoplasms
    157 rdf:type schema:DefinedTerm
    158 Na5972ba8ca7243579cd40a15afb53175 rdf:first sg:person.01045237730.27
    159 rdf:rest N383dcbebf0964b8e8ff30c64efdb6ec8
    160 Nabf90713426a47fd96bf9d272e504a02 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    161 schema:name Gene Knockout Techniques
    162 rdf:type schema:DefinedTerm
    163 Nacb5c9aa734644a6bb61134ad5c7288f rdf:first sg:person.0616026301.65
    164 rdf:rest N1a57d4f8427a4bed8072c1bd8298f9de
    165 Nb47f081a7a624fe1b734c8401d4b220b rdf:first sg:person.01225204001.22
    166 rdf:rest Ne75520daa3e3439d941624a1f4cdfecc
    167 Nb7c800864b394498978ad8d04a8806d1 schema:name nlm_unique_id
    168 schema:value 101563288
    169 rdf:type schema:PropertyValue
    170 Nb9fc69a7f29b40ebb1b19c04c979b39c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    171 schema:name Gene Editing
    172 rdf:type schema:DefinedTerm
    173 Nbf59366f196a47b19cddca3857eadf4d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    174 schema:name Transfection
    175 rdf:type schema:DefinedTerm
    176 Nc30df37fe2e740bba74597fabdac154a rdf:first sg:person.0660300401.82
    177 rdf:rest N7e24d55d38434e4bbe8afa84ead2f933
    178 Nc6fd0630b313412594752c73228b1b1c rdf:first sg:person.01010403631.18
    179 rdf:rest N300dadea62f9482ab0a1f2eb0d88b092
    180 Nc7c2d2e26b2748c7ab9e2e78d79f2ee6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    181 schema:name RNA, Guide
    182 rdf:type schema:DefinedTerm
    183 Nc7d3fd32562d4abe862e8ab4616e0786 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    184 schema:name Genetic Vectors
    185 rdf:type schema:DefinedTerm
    186 Ncacaf2f484dd457c88f46065939995a2 schema:volumeNumber 6
    187 rdf:type schema:PublicationVolume
    188 Nd3572d088fc94f788dd51ba9f0fe0aa3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    189 schema:name Gene Transfer Techniques
    190 rdf:type schema:DefinedTerm
    191 Nd737c4746483458da9c8528ebf773029 schema:name pubmed_id
    192 schema:value 26818188
    193 rdf:type schema:PropertyValue
    194 Ne75520daa3e3439d941624a1f4cdfecc rdf:first sg:person.01273317201.05
    195 rdf:rest Nfe90120020764b79b8ec507507181db0
    196 Nfe90120020764b79b8ec507507181db0 rdf:first sg:person.01322162704.14
    197 rdf:rest Nacb5c9aa734644a6bb61134ad5c7288f
    198 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    199 schema:name Medical and Health Sciences
    200 rdf:type schema:DefinedTerm
    201 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
    202 schema:name Immunology
    203 rdf:type schema:DefinedTerm
    204 sg:grant.5008637 http://pending.schema.org/fundedItem sg:pub.10.1038/srep20070
    205 rdf:type schema:MonetaryGrant
    206 sg:grant.6993090 http://pending.schema.org/fundedItem sg:pub.10.1038/srep20070
    207 rdf:type schema:MonetaryGrant
    208 sg:grant.7023314 http://pending.schema.org/fundedItem sg:pub.10.1038/srep20070
    209 rdf:type schema:MonetaryGrant
    210 sg:journal.1045337 schema:issn 2045-2322
    211 schema:name Scientific Reports
    212 rdf:type schema:Periodical
    213 sg:person.01010403631.18 schema:affiliation https://www.grid.ac/institutes/grid.452564.4
    214 schema:familyName Du
    215 schema:givenName Yinan
    216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010403631.18
    217 rdf:type schema:Person
    218 sg:person.01024251730.25 schema:affiliation https://www.grid.ac/institutes/grid.452564.4
    219 schema:familyName Zhou
    220 schema:givenName Jiankui
    221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01024251730.25
    222 rdf:type schema:Person
    223 sg:person.01045237730.27 schema:affiliation https://www.grid.ac/institutes/grid.428392.6
    224 schema:familyName Zou
    225 schema:givenName Zhengyun
    226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01045237730.27
    227 rdf:type schema:Person
    228 sg:person.01163153766.89 schema:affiliation https://www.grid.ac/institutes/grid.428392.6
    229 schema:familyName Meng
    230 schema:givenName Fanyan
    231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163153766.89
    232 rdf:type schema:Person
    233 sg:person.01225204001.22 schema:affiliation https://www.grid.ac/institutes/grid.428392.6
    234 schema:familyName Zhang
    235 schema:givenName Lianru
    236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01225204001.22
    237 rdf:type schema:Person
    238 sg:person.01232700601.24 schema:affiliation https://www.grid.ac/institutes/grid.428392.6
    239 schema:familyName Liu
    240 schema:givenName Baorui
    241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01232700601.24
    242 rdf:type schema:Person
    243 sg:person.01273317201.05 schema:affiliation https://www.grid.ac/institutes/grid.428392.6
    244 schema:familyName Chen
    245 schema:givenName Fangjun
    246 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01273317201.05
    247 rdf:type schema:Person
    248 sg:person.01322162704.14 schema:affiliation https://www.grid.ac/institutes/grid.428392.6
    249 schema:familyName Sha
    250 schema:givenName Huizi
    251 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322162704.14
    252 rdf:type schema:Person
    253 sg:person.01335571301.60 schema:affiliation https://www.grid.ac/institutes/grid.428392.6
    254 schema:familyName Su
    255 schema:givenName Shu
    256 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01335571301.60
    257 rdf:type schema:Person
    258 sg:person.0616026301.65 schema:affiliation https://www.grid.ac/institutes/grid.428392.6
    259 schema:familyName Cheng
    260 schema:givenName Lei
    261 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616026301.65
    262 rdf:type schema:Person
    263 sg:person.0660300401.82 schema:affiliation https://www.grid.ac/institutes/grid.428392.6
    264 schema:familyName Shao
    265 schema:givenName Jie
    266 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0660300401.82
    267 rdf:type schema:Person
    268 sg:person.0674155231.70 schema:affiliation https://www.grid.ac/institutes/grid.89957.3a
    269 schema:familyName Shen
    270 schema:givenName Bin
    271 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0674155231.70
    272 rdf:type schema:Person
    273 sg:person.0714167670.35 schema:affiliation https://www.grid.ac/institutes/grid.452564.4
    274 schema:familyName Hu
    275 schema:givenName Bian
    276 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714167670.35
    277 rdf:type schema:Person
    278 sg:person.0715553130.19 schema:affiliation https://www.grid.ac/institutes/grid.428392.6
    279 schema:familyName Yu
    280 schema:givenName Lixia
    281 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715553130.19
    282 rdf:type schema:Person
    283 sg:person.0730336324.63 schema:affiliation https://www.grid.ac/institutes/grid.440637.2
    284 schema:familyName Huang
    285 schema:givenName Xingxu
    286 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0730336324.63
    287 rdf:type schema:Person
    288 sg:person.0774527001.50 schema:affiliation https://www.grid.ac/institutes/grid.428392.6
    289 schema:familyName Du
    290 schema:givenName Juan
    291 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0774527001.50
    292 rdf:type schema:Person
    293 sg:pub.10.1007/s00262-010-0842-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041143302
    294 https://doi.org/10.1007/s00262-010-0842-0
    295 rdf:type schema:CreativeWork
    296 sg:pub.10.1038/cr.2013.157 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017624741
    297 https://doi.org/10.1038/cr.2013.157
    298 rdf:type schema:CreativeWork
    299 sg:pub.10.1038/cr.2013.46 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038811201
    300 https://doi.org/10.1038/cr.2013.46
    301 rdf:type schema:CreativeWork
    302 sg:pub.10.1038/gt.2011.185 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029720470
    303 https://doi.org/10.1038/gt.2011.185
    304 rdf:type schema:CreativeWork
    305 sg:pub.10.1038/gt.2013.80 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017529500
    306 https://doi.org/10.1038/gt.2013.80
    307 rdf:type schema:CreativeWork
    308 sg:pub.10.1038/nature13954 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020455470
    309 https://doi.org/10.1038/nature13954
    310 rdf:type schema:CreativeWork
    311 sg:pub.10.1038/nbt.2652 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048853216
    312 https://doi.org/10.1038/nbt.2652
    313 rdf:type schema:CreativeWork
    314 sg:pub.10.1038/ncponc0098 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026358991
    315 https://doi.org/10.1038/ncponc0098
    316 rdf:type schema:CreativeWork
    317 sg:pub.10.1038/nm.2700 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050105684
    318 https://doi.org/10.1038/nm.2700
    319 rdf:type schema:CreativeWork
    320 sg:pub.10.1038/nm.3105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008050192
    321 https://doi.org/10.1038/nm.3105
    322 rdf:type schema:CreativeWork
    323 sg:pub.10.1038/nm1100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001965372
    324 https://doi.org/10.1038/nm1100
    325 rdf:type schema:CreativeWork
    326 sg:pub.10.1038/nm942 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008593772
    327 https://doi.org/10.1038/nm942
    328 rdf:type schema:CreativeWork
    329 sg:pub.10.1038/nmeth.2649 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008703730
    330 https://doi.org/10.1038/nmeth.2649
    331 rdf:type schema:CreativeWork
    332 sg:pub.10.1038/nmeth.2857 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035757498
    333 https://doi.org/10.1038/nmeth.2857
    334 rdf:type schema:CreativeWork
    335 sg:pub.10.1038/nrc3239 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047946992
    336 https://doi.org/10.1038/nrc3239
    337 rdf:type schema:CreativeWork
    338 sg:pub.10.1385/ir:24:2:201 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008620959
    339 https://doi.org/10.1385/ir:24:2:201
    340 rdf:type schema:CreativeWork
    341 https://doi.org/10.1002/ijc.21775 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022291558
    342 rdf:type schema:CreativeWork
    343 https://doi.org/10.1016/j.cell.2014.01.027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046173829
    344 rdf:type schema:CreativeWork
    345 https://doi.org/10.1056/nejmoa1200690 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042896583
    346 rdf:type schema:CreativeWork
    347 https://doi.org/10.1056/nejmoa1200694 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047413837
    348 rdf:type schema:CreativeWork
    349 https://doi.org/10.1073/pnas.1100213108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041154581
    350 rdf:type schema:CreativeWork
    351 https://doi.org/10.1093/intimm/dxm091 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015532387
    352 rdf:type schema:CreativeWork
    353 https://doi.org/10.1093/intimm/dxp072 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051487407
    354 rdf:type schema:CreativeWork
    355 https://doi.org/10.1093/nar/gks1446 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047402217
    356 rdf:type schema:CreativeWork
    357 https://doi.org/10.1097/01.cji.0000162780.96310.e4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044545822
    358 rdf:type schema:CreativeWork
    359 https://doi.org/10.1126/science.1225829 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041850060
    360 rdf:type schema:CreativeWork
    361 https://doi.org/10.1146/annurev.immunol.26.021607.090331 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005008646
    362 rdf:type schema:CreativeWork
    363 https://doi.org/10.1158/0008-5472.can-14-1258 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001080038
    364 rdf:type schema:CreativeWork
    365 https://doi.org/10.1158/1078-0432.ccr-07-4079 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047225303
    366 rdf:type schema:CreativeWork
    367 https://doi.org/10.1158/1078-0432.ccr-13-0458 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046840369
    368 rdf:type schema:CreativeWork
    369 https://doi.org/10.1371/journal.pmed.0010028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018097004
    370 rdf:type schema:CreativeWork
    371 https://doi.org/10.1371/journal.pone.0060298 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040501714
    372 rdf:type schema:CreativeWork
    373 https://doi.org/10.1371/journal.pone.0064138 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017756173
    374 rdf:type schema:CreativeWork
    375 https://doi.org/10.18632/oncotarget.2980 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001783473
    376 rdf:type schema:CreativeWork
    377 https://doi.org/10.3389/fimmu.2013.00221 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038614191
    378 rdf:type schema:CreativeWork
    379 https://doi.org/10.7555/jbr.28.20140066 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013110661
    380 rdf:type schema:CreativeWork
    381 https://www.grid.ac/institutes/grid.428392.6 schema:alternateName Nanjing Drum Tower Hospital
    382 schema:name The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China
    383 rdf:type schema:Organization
    384 https://www.grid.ac/institutes/grid.440637.2 schema:alternateName ShanghaiTech University
    385 schema:name MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, National Resource Center for Mutant Mice, Nanjing 210061, China
    386 School of Life Science and Technology, ShanghaiTech University, 100 Haike Rd., Pudong New Area, Shanghai 201210, China
    387 rdf:type schema:Organization
    388 https://www.grid.ac/institutes/grid.452564.4 schema:alternateName Model Animal Research Center
    389 schema:name MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, National Resource Center for Mutant Mice, Nanjing 210061, China
    390 rdf:type schema:Organization
    391 https://www.grid.ac/institutes/grid.89957.3a schema:alternateName Nanjing Medical University
    392 schema:name State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China
    393 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...