Constructive remodeling of a synthetic endothelial extracellular matrix View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-11

AUTHORS

Sewoon Han, Yoojin Shin, Hyo Eun Jeong, Jessie S Jeon, Roger D Kamm, Dongeun Huh, Lydia L Sohn, Seok Chung

ABSTRACT

The construction of well-controllable in vitro models of physiological and pathological vascular endothelium remains a fundamental challenge in tissue engineering and drug development. Here, we present an approach for forming a synthetic endothelial extracellular matrix (ECM) that closely resembles that of the native structure by locally depositing basement membrane materials onto type 1 collagen nanofibers only in a region adjacent to the endothelial cell (EC) monolayer. Culturing the EC monolayer on this synthetic endothelial ECM remarkably enhanced its physiological properties, reducing its vascular permeability, and promoting a stabilized, quiescent phenotype. We demonstrated that the EC monolayer on the synthetic endothelial ECM neither creates non-physiological barriers to cell-cell or cell-ECM interactions, nor hinders molecular diffusion of growth factors and other molecules. The synthetic endothelial ECM and vascular endothelium on it may help us enter in a new phase of research in which various models of the biological barrier behavior can be tested experimentally. More... »

PAGES

18290

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep18290

DOI

http://dx.doi.org/10.1038/srep18290

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045651193

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26687334


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