A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-12-10

AUTHORS

Alessio Polacchini, Giuliana Metelli, Ruggiero Francavilla, Gabriele Baj, Marina Florean, Luca Giovanni Mascaretti, Enrico Tongiorgi

ABSTRACT

Brain-Derived Neurotrophic Factor (BDNF) has attracted increasing interest as potential biomarker to support the diagnosis or monitor the efficacy of therapies in brain disorders. Circulating BDNF can be measured in serum, plasma or whole blood. However, the use of BDNF as biomarker is limited by the poor reproducibility of results, likely due to the variety of methods used for sample collection and BDNF analysis. To overcome these limitations, using sera from 40 healthy adults, we compared the performance of five ELISA kits (Aviscera-Bioscience, Biosensis, Millipore-ChemiKine(TM), Promega-Emax(®), R&D-System-Quantikine(®)) and one multiplexing assay (Millipore-Milliplex(®)). All kits showed 100% sample recovery and comparable range. However, they exhibited very different inter-assay variations from 5% to 20%. Inter-assay variations were higher than those declared by the manufacturers with only one exception which also had the best overall performance. Dot-blot analysis revealed that two kits selectively recognize mature BDNF, while the others reacted with both pro-BDNF and mature BDNF. In conclusion, we identified two assays to obtain reliable measurements of human serum BDNF, suitable for future clinical applications. More... »

PAGES

17989

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep17989

DOI

http://dx.doi.org/10.1038/srep17989

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038360337

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26656852


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