Hepatitis B virus efficiently infects non-adherent hepatoma cells via human sodium taurocholate cotransporting polypeptide View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-12

AUTHORS

Kaori Okuyama-Dobashi, Hirotake Kasai, Tomohisa Tanaka, Atsuya Yamashita, Jun Yasumoto, Wenjia Chen, Toru Okamoto, Shinya Maekawa, Koichi Watashi, Takaji Wakita, Akihide Ryo, Tetsuro Suzuki, Yoshiharu Matsuura, Nobuyuki Enomoto, Kohji Moriishi

ABSTRACT

Sodium taurocholate cotransporting polypeptide (NTCP) has been reported as a functional receptor for hepatitis B virus (HBV) infection. However, HBV could not efficiently infect HepG2 cells expressing NTCP (NTCP-HepG2 cells) under adherent monolayer-cell conditions. In this study, NTCP was mainly detected in the basolateral membrane region, but not the apical site, of monolayer NTCP-HepG2 cells. We hypothesized that non-adherent cell conditions of infection would enhance HBV infectivity. Non-adherent NTCP-HepG2 cells were prepared by treatment with trypsin and EDTA, which did not degrade NTCP in the membrane fraction. HBV successfully infected NTCP-HepG2 cells at a viral dose 10 times lower in non-adherent phase than in adherent phase. Efficient infection of non-adherent NTCP-HepG2 cells with blood-borne or cell-culture-derived HBV was observed and was remarkably impaired in the presence of the myristoylated preS1 peptide. HBV could also efficiently infect HepaRG cells under non-adherent cell conditions. We screened several compounds using our culture system and identified proscillaridin A as a potent anti-HBV agent with an IC50 value of 7.2 nM. In conclusion, non-adherent host cell conditions of infection augmented HBV infectivity in an NTCP-dependent manner, thus providing a novel strategy to identify anti-HBV drugs and investigate the mechanism of HBV infection. More... »

PAGES

17047

References to SciGraph publications

  • 2011-05. Hepatitis B therapy in NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/srep17047

    DOI

    http://dx.doi.org/10.1038/srep17047

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1039304659

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26592202


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