A simple and predictive phenotypic High Content Imaging assay for Plasmodium falciparum mature gametocytes to identify malaria transmission blocking compounds View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-12

AUTHORS

Leonardo Lucantoni, Francesco Silvestrini, Michele Signore, Giulia Siciliano, Maarten Eldering, Koen J. Dechering, Vicky M. Avery, Pietro Alano

ABSTRACT

Plasmodium falciparum gametocytes, specifically the mature stages, are the only malaria parasite stage in humans transmissible to the mosquito vector. Anti-malarial drugs capable of killing these forms are considered essential for the eradication of malaria and tools allowing the screening of large compound libraries with high predictive power are needed to identify new candidates. As gametocytes are not a replicative stage it is difficult to apply the same drug screening methods used for asexual stages. Here we propose an assay, based on high content imaging, combining "classic" gametocyte viability readout based on gametocyte counts with a functional viability readout, based on gametocyte activation and the discrimination of the typical gamete spherical morphology. This simple and rapid assay has been miniaturized to a 384-well format using acridine orange staining of wild type P. falciparum 3D7A sexual forms, and was validated by screening reference antimalarial drugs and the MMV Malaria Box. The assay demonstrated excellent robustness and ability to identify quality hits with high likelihood of confirmation of transmission reducing activity in subsequent mosquito membrane feeding assays. More... »

PAGES

16414

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/srep16414

DOI

http://dx.doi.org/10.1038/srep16414

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1039452888

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26553647


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antimalarials", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Evaluation, Preclinical", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "High-Throughput Screening Assays", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Malaria, Falciparum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Confocal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phenotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmodium falciparum", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Griffith University", 
          "id": "https://www.grid.ac/institutes/grid.1022.1", 
          "name": [
            "Discovery Biology, Eskitis Institute for Drug Discovery, Griffith University, 4111 Nathan, Queensland, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lucantoni", 
        "givenName": "Leonardo", 
        "id": "sg:person.01357364703.87", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01357364703.87"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Istituto Superiore di Sanit\u00e0", 
          "id": "https://www.grid.ac/institutes/grid.416651.1", 
          "name": [
            "Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanit\u00e0, Viale Regina Elena n. 299, 00161 Roma, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Silvestrini", 
        "givenName": "Francesco", 
        "id": "sg:person.0724473766.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724473766.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Istituto Superiore di Sanit\u00e0", 
          "id": "https://www.grid.ac/institutes/grid.416651.1", 
          "name": [
            "Dipartimento di Ematologia, Oncologia e Medicina Molecolare, Istituto Superiore di Sanit\u00e0, Viale Regina Elena n. 299, 00161 Roma, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Signore", 
        "givenName": "Michele", 
        "id": "sg:person.01301566760.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01301566760.86"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Istituto Superiore di Sanit\u00e0", 
          "id": "https://www.grid.ac/institutes/grid.416651.1", 
          "name": [
            "Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanit\u00e0, Viale Regina Elena n. 299, 00161 Roma, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Siciliano", 
        "givenName": "Giulia", 
        "id": "sg:person.01111220620.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111220620.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "TropIQ Health Sciences", 
          "id": "https://www.grid.ac/institutes/grid.475691.8", 
          "name": [
            "TropIQ Health Sciences, Geert Grooteplein 28, huispost 268, 6525 GA Nijmegen, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Eldering", 
        "givenName": "Maarten", 
        "id": "sg:person.01150403140.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150403140.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "TropIQ Health Sciences", 
          "id": "https://www.grid.ac/institutes/grid.475691.8", 
          "name": [
            "TropIQ Health Sciences, Geert Grooteplein 28, huispost 268, 6525 GA Nijmegen, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dechering", 
        "givenName": "Koen J.", 
        "id": "sg:person.0750244570.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750244570.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Griffith University", 
          "id": "https://www.grid.ac/institutes/grid.1022.1", 
          "name": [
            "Discovery Biology, Eskitis Institute for Drug Discovery, Griffith University, 4111 Nathan, Queensland, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Avery", 
        "givenName": "Vicky M.", 
        "id": "sg:person.01001000051.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001000051.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Istituto Superiore di Sanit\u00e0", 
          "id": "https://www.grid.ac/institutes/grid.416651.1", 
          "name": [
            "Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanit\u00e0, Viale Regina Elena n. 299, 00161 Roma, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Alano", 
        "givenName": "Pietro", 
        "id": "sg:person.01272465174.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272465174.13"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1371/journal.pone.0093825", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005650784"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.03666-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006596920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.04684-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008164211"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/644645", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009646480"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa0808859", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011738279"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pmed.1001169", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018462267"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jac/dkt165", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020302042"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2875-12-408", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020375561", 
          "https://doi.org/10.1186/1475-2875-12-408"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0105817", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020586455"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.01500-13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021098123"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0035019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021371514"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1098/rspb.1978.0051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021626314"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1260403", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022075703"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jac/dkv003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024993976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2875-9-49", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025444168", 
          "https://doi.org/10.1186/1475-2875-9-49"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2010/976827", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025595177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0031182013001236", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025615017"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep03743", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026373964", 
          "https://doi.org/10.1038/srep03743"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/ec.00186-09", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027379745"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0014470", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028288618"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.00325-13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028605117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.03772-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030742443"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.00870-13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031076048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pbio.0060139", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034167239"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1469-0691.2011.03664.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035587988"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molbiopara.2011.02.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036364762"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2875-9-136", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036677225", 
          "https://doi.org/10.1186/1475-2875-9-136"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0062906", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038088492"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/108705719900400206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038278323"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/108705719900400206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038278323"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.00709-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043125528"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac502098w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044451958"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2008.06267.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046683454"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1112037108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047538572"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.febslet.2011.04.042", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047864135"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/32667", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047956353", 
          "https://doi.org/10.1038/32667"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/32667", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047956353", 
          "https://doi.org/10.1038/32667"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/cmr.00051-10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048527307"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0035-9203(01)90016-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049542735"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/infdis/jiu271", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050347263"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.04332-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051759496"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4269/ajtmh.2012.11-0302", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052099330"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4269/ajtmh.2012.11-0302", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052099330"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4269/ajtmh.2012.11-0302", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052099330"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.781840", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062649809"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2015-12", 
    "datePublishedReg": "2015-12-01", 
    "description": "Plasmodium falciparum gametocytes, specifically the mature stages, are the only malaria parasite stage in humans transmissible to the mosquito vector. Anti-malarial drugs capable of killing these forms are considered essential for the eradication of malaria and tools allowing the screening of large compound libraries with high predictive power are needed to identify new candidates. As gametocytes are not a replicative stage it is difficult to apply the same drug screening methods used for asexual stages. Here we propose an assay, based on high content imaging, combining \"classic\" gametocyte viability readout based on gametocyte counts with a functional viability readout, based on gametocyte activation and the discrimination of the typical gamete spherical morphology. This simple and rapid assay has been miniaturized to a 384-well format using acridine orange staining of wild type P. falciparum 3D7A sexual forms, and was validated by screening reference antimalarial drugs and the MMV Malaria Box. The assay demonstrated excellent robustness and ability to identify quality hits with high likelihood of confirmation of transmission reducing activity in subsequent mosquito membrane feeding assays. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/srep16414", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.6585936", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6581083", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "5"
      }
    ], 
    "name": "A simple and predictive phenotypic High Content Imaging assay for Plasmodium falciparum mature gametocytes to identify malaria transmission blocking compounds", 
    "pagination": "16414", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "bbef83f87e7e9ea241a5bf17b3e8f6ad58ce6814bc395e34e77f2042a45a4857"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "26553647"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/srep16414"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1039452888"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/srep16414", 
      "https://app.dimensions.ai/details/publication/pub.1039452888"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:38", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000425.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/srep16414"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/srep16414'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/srep16414'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/srep16414'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/srep16414'


 

This table displays all metadata directly associated to this object as RDF triples.

288 TRIPLES      21 PREDICATES      78 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/srep16414 schema:about N2fee06c0348d4509ba00ab5ebc7c99be
2 N42431899b36a41b6aa7e55a8a34f371c
3 N4f68231a01f2448586c8ef671864cabb
4 N56823347b8204f9792ce2ef127f381b8
5 Nc0f68cba6059485dbb9bd0b06fbec9f0
6 Nc107c22561c54ba1bab92878795f52b4
7 Ne8d1ec5da9854537825f6c851a57a15d
8 Nf050953572e34c3388adf6038d6fc72c
9 anzsrc-for:11
10 anzsrc-for:1108
11 schema:author N448a8a6f141944648d19cdacbeabd22e
12 schema:citation sg:pub.10.1038/32667
13 sg:pub.10.1038/srep03743
14 sg:pub.10.1186/1475-2875-12-408
15 sg:pub.10.1186/1475-2875-9-136
16 sg:pub.10.1186/1475-2875-9-49
17 https://doi.org/10.1016/j.febslet.2011.04.042
18 https://doi.org/10.1016/j.molbiopara.2011.02.005
19 https://doi.org/10.1016/s0035-9203(01)90016-1
20 https://doi.org/10.1017/s0031182013001236
21 https://doi.org/10.1021/ac502098w
22 https://doi.org/10.1056/nejmoa0808859
23 https://doi.org/10.1073/pnas.1112037108
24 https://doi.org/10.1086/644645
25 https://doi.org/10.1093/infdis/jiu271
26 https://doi.org/10.1093/jac/dkt165
27 https://doi.org/10.1093/jac/dkv003
28 https://doi.org/10.1098/rspb.1978.0051
29 https://doi.org/10.1111/j.1365-2958.2008.06267.x
30 https://doi.org/10.1111/j.1469-0691.2011.03664.x
31 https://doi.org/10.1126/science.1260403
32 https://doi.org/10.1126/science.781840
33 https://doi.org/10.1128/aac.00325-13
34 https://doi.org/10.1128/aac.00709-12
35 https://doi.org/10.1128/aac.00870-13
36 https://doi.org/10.1128/aac.01500-13
37 https://doi.org/10.1128/aac.03666-14
38 https://doi.org/10.1128/aac.03772-14
39 https://doi.org/10.1128/aac.04332-14
40 https://doi.org/10.1128/aac.04684-14
41 https://doi.org/10.1128/cmr.00051-10
42 https://doi.org/10.1128/ec.00186-09
43 https://doi.org/10.1155/2010/976827
44 https://doi.org/10.1177/108705719900400206
45 https://doi.org/10.1371/journal.pbio.0060139
46 https://doi.org/10.1371/journal.pmed.1001169
47 https://doi.org/10.1371/journal.pone.0014470
48 https://doi.org/10.1371/journal.pone.0035019
49 https://doi.org/10.1371/journal.pone.0062906
50 https://doi.org/10.1371/journal.pone.0093825
51 https://doi.org/10.1371/journal.pone.0105817
52 https://doi.org/10.4269/ajtmh.2012.11-0302
53 schema:datePublished 2015-12
54 schema:datePublishedReg 2015-12-01
55 schema:description Plasmodium falciparum gametocytes, specifically the mature stages, are the only malaria parasite stage in humans transmissible to the mosquito vector. Anti-malarial drugs capable of killing these forms are considered essential for the eradication of malaria and tools allowing the screening of large compound libraries with high predictive power are needed to identify new candidates. As gametocytes are not a replicative stage it is difficult to apply the same drug screening methods used for asexual stages. Here we propose an assay, based on high content imaging, combining "classic" gametocyte viability readout based on gametocyte counts with a functional viability readout, based on gametocyte activation and the discrimination of the typical gamete spherical morphology. This simple and rapid assay has been miniaturized to a 384-well format using acridine orange staining of wild type P. falciparum 3D7A sexual forms, and was validated by screening reference antimalarial drugs and the MMV Malaria Box. The assay demonstrated excellent robustness and ability to identify quality hits with high likelihood of confirmation of transmission reducing activity in subsequent mosquito membrane feeding assays.
56 schema:genre research_article
57 schema:inLanguage en
58 schema:isAccessibleForFree true
59 schema:isPartOf N2a7097fc27404b379adbca0729d3afae
60 N9464be28b6604155a33d0dd9f87a49c2
61 sg:journal.1045337
62 schema:name A simple and predictive phenotypic High Content Imaging assay for Plasmodium falciparum mature gametocytes to identify malaria transmission blocking compounds
63 schema:pagination 16414
64 schema:productId N3d3928c57d504c09bf6618ae3a297225
65 N573d1b080db345aaa0c6a60474546278
66 N71a28edd2219465e9160925f73629473
67 Nce8840a3ecd84d2898b900858e0ed844
68 Nddf2ccd8aff14b2ebf602de69de7c39f
69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039452888
70 https://doi.org/10.1038/srep16414
71 schema:sdDatePublished 2019-04-10T15:38
72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
73 schema:sdPublisher Nfdc7c83d44914a6b891f8a47c2049c00
74 schema:url http://www.nature.com/articles/srep16414
75 sgo:license sg:explorer/license/
76 sgo:sdDataset articles
77 rdf:type schema:ScholarlyArticle
78 N18b68957dbd8406eba32ff0f5d974e34 rdf:first sg:person.01001000051.25
79 rdf:rest N5046ddccc312466aa978cfae3adc2692
80 N2a7097fc27404b379adbca0729d3afae schema:volumeNumber 5
81 rdf:type schema:PublicationVolume
82 N2fee06c0348d4509ba00ab5ebc7c99be schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Phenotype
84 rdf:type schema:DefinedTerm
85 N3d3928c57d504c09bf6618ae3a297225 schema:name dimensions_id
86 schema:value pub.1039452888
87 rdf:type schema:PropertyValue
88 N42431899b36a41b6aa7e55a8a34f371c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Humans
90 rdf:type schema:DefinedTerm
91 N448a8a6f141944648d19cdacbeabd22e rdf:first sg:person.01357364703.87
92 rdf:rest Ne0f9f068d3a44865bb68fffe917a1edd
93 N4f68231a01f2448586c8ef671864cabb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Microscopy, Confocal
95 rdf:type schema:DefinedTerm
96 N5046ddccc312466aa978cfae3adc2692 rdf:first sg:person.01272465174.13
97 rdf:rest rdf:nil
98 N56823347b8204f9792ce2ef127f381b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Malaria, Falciparum
100 rdf:type schema:DefinedTerm
101 N573d1b080db345aaa0c6a60474546278 schema:name pubmed_id
102 schema:value 26553647
103 rdf:type schema:PropertyValue
104 N71a28edd2219465e9160925f73629473 schema:name readcube_id
105 schema:value bbef83f87e7e9ea241a5bf17b3e8f6ad58ce6814bc395e34e77f2042a45a4857
106 rdf:type schema:PropertyValue
107 N9464be28b6604155a33d0dd9f87a49c2 schema:issueNumber 1
108 rdf:type schema:PublicationIssue
109 N972341e5de104a64894249934b38b1a8 rdf:first sg:person.0750244570.01
110 rdf:rest N18b68957dbd8406eba32ff0f5d974e34
111 Na41f7fd0bfb34056bf045acc811b3ffa rdf:first sg:person.01150403140.20
112 rdf:rest N972341e5de104a64894249934b38b1a8
113 Nb5771b00350f4c81a81692366e795921 rdf:first sg:person.01111220620.19
114 rdf:rest Na41f7fd0bfb34056bf045acc811b3ffa
115 Nbb8a2fce072d4577936b4013bd982caf rdf:first sg:person.01301566760.86
116 rdf:rest Nb5771b00350f4c81a81692366e795921
117 Nc0f68cba6059485dbb9bd0b06fbec9f0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Drug Evaluation, Preclinical
119 rdf:type schema:DefinedTerm
120 Nc107c22561c54ba1bab92878795f52b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Antimalarials
122 rdf:type schema:DefinedTerm
123 Nce8840a3ecd84d2898b900858e0ed844 schema:name doi
124 schema:value 10.1038/srep16414
125 rdf:type schema:PropertyValue
126 Nddf2ccd8aff14b2ebf602de69de7c39f schema:name nlm_unique_id
127 schema:value 101563288
128 rdf:type schema:PropertyValue
129 Ne0f9f068d3a44865bb68fffe917a1edd rdf:first sg:person.0724473766.47
130 rdf:rest Nbb8a2fce072d4577936b4013bd982caf
131 Ne8d1ec5da9854537825f6c851a57a15d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Plasmodium falciparum
133 rdf:type schema:DefinedTerm
134 Nf050953572e34c3388adf6038d6fc72c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name High-Throughput Screening Assays
136 rdf:type schema:DefinedTerm
137 Nfdc7c83d44914a6b891f8a47c2049c00 schema:name Springer Nature - SN SciGraph project
138 rdf:type schema:Organization
139 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
140 schema:name Medical and Health Sciences
141 rdf:type schema:DefinedTerm
142 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
143 schema:name Medical Microbiology
144 rdf:type schema:DefinedTerm
145 sg:grant.6581083 http://pending.schema.org/fundedItem sg:pub.10.1038/srep16414
146 rdf:type schema:MonetaryGrant
147 sg:grant.6585936 http://pending.schema.org/fundedItem sg:pub.10.1038/srep16414
148 rdf:type schema:MonetaryGrant
149 sg:journal.1045337 schema:issn 2045-2322
150 schema:name Scientific Reports
151 rdf:type schema:Periodical
152 sg:person.01001000051.25 schema:affiliation https://www.grid.ac/institutes/grid.1022.1
153 schema:familyName Avery
154 schema:givenName Vicky M.
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001000051.25
156 rdf:type schema:Person
157 sg:person.01111220620.19 schema:affiliation https://www.grid.ac/institutes/grid.416651.1
158 schema:familyName Siciliano
159 schema:givenName Giulia
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111220620.19
161 rdf:type schema:Person
162 sg:person.01150403140.20 schema:affiliation https://www.grid.ac/institutes/grid.475691.8
163 schema:familyName Eldering
164 schema:givenName Maarten
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150403140.20
166 rdf:type schema:Person
167 sg:person.01272465174.13 schema:affiliation https://www.grid.ac/institutes/grid.416651.1
168 schema:familyName Alano
169 schema:givenName Pietro
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272465174.13
171 rdf:type schema:Person
172 sg:person.01301566760.86 schema:affiliation https://www.grid.ac/institutes/grid.416651.1
173 schema:familyName Signore
174 schema:givenName Michele
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01301566760.86
176 rdf:type schema:Person
177 sg:person.01357364703.87 schema:affiliation https://www.grid.ac/institutes/grid.1022.1
178 schema:familyName Lucantoni
179 schema:givenName Leonardo
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01357364703.87
181 rdf:type schema:Person
182 sg:person.0724473766.47 schema:affiliation https://www.grid.ac/institutes/grid.416651.1
183 schema:familyName Silvestrini
184 schema:givenName Francesco
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724473766.47
186 rdf:type schema:Person
187 sg:person.0750244570.01 schema:affiliation https://www.grid.ac/institutes/grid.475691.8
188 schema:familyName Dechering
189 schema:givenName Koen J.
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750244570.01
191 rdf:type schema:Person
192 sg:pub.10.1038/32667 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047956353
193 https://doi.org/10.1038/32667
194 rdf:type schema:CreativeWork
195 sg:pub.10.1038/srep03743 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026373964
196 https://doi.org/10.1038/srep03743
197 rdf:type schema:CreativeWork
198 sg:pub.10.1186/1475-2875-12-408 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020375561
199 https://doi.org/10.1186/1475-2875-12-408
200 rdf:type schema:CreativeWork
201 sg:pub.10.1186/1475-2875-9-136 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036677225
202 https://doi.org/10.1186/1475-2875-9-136
203 rdf:type schema:CreativeWork
204 sg:pub.10.1186/1475-2875-9-49 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025444168
205 https://doi.org/10.1186/1475-2875-9-49
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/j.febslet.2011.04.042 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047864135
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/j.molbiopara.2011.02.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036364762
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/s0035-9203(01)90016-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049542735
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1017/s0031182013001236 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025615017
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1021/ac502098w schema:sameAs https://app.dimensions.ai/details/publication/pub.1044451958
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1056/nejmoa0808859 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011738279
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1073/pnas.1112037108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047538572
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1086/644645 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009646480
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1093/infdis/jiu271 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050347263
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1093/jac/dkt165 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020302042
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1093/jac/dkv003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024993976
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1098/rspb.1978.0051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021626314
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1111/j.1365-2958.2008.06267.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1046683454
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1111/j.1469-0691.2011.03664.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1035587988
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1126/science.1260403 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022075703
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1126/science.781840 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062649809
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1128/aac.00325-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028605117
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1128/aac.00709-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043125528
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1128/aac.00870-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031076048
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1128/aac.01500-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021098123
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1128/aac.03666-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006596920
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1128/aac.03772-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030742443
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1128/aac.04332-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051759496
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1128/aac.04684-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008164211
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1128/cmr.00051-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048527307
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1128/ec.00186-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027379745
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1155/2010/976827 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025595177
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1177/108705719900400206 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038278323
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1371/journal.pbio.0060139 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034167239
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1371/journal.pmed.1001169 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018462267
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1371/journal.pone.0014470 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028288618
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1371/journal.pone.0035019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021371514
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1371/journal.pone.0062906 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038088492
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1371/journal.pone.0093825 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005650784
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1371/journal.pone.0105817 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020586455
276 rdf:type schema:CreativeWork
277 https://doi.org/10.4269/ajtmh.2012.11-0302 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052099330
278 rdf:type schema:CreativeWork
279 https://www.grid.ac/institutes/grid.1022.1 schema:alternateName Griffith University
280 schema:name Discovery Biology, Eskitis Institute for Drug Discovery, Griffith University, 4111 Nathan, Queensland, Australia
281 rdf:type schema:Organization
282 https://www.grid.ac/institutes/grid.416651.1 schema:alternateName Istituto Superiore di Sanità
283 schema:name Dipartimento di Ematologia, Oncologia e Medicina Molecolare, Istituto Superiore di Sanità, Viale Regina Elena n. 299, 00161 Roma, Italy
284 Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanità, Viale Regina Elena n. 299, 00161 Roma, Italy
285 rdf:type schema:Organization
286 https://www.grid.ac/institutes/grid.475691.8 schema:alternateName TropIQ Health Sciences
287 schema:name TropIQ Health Sciences, Geert Grooteplein 28, huispost 268, 6525 GA Nijmegen, The Netherlands
288 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...